FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l02n01 ah8335b-062415.3410000000e289.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l02n01 ah8335b-062415.3410000000e289.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12571578
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA360990.2871477232213808No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC294120.233956310019315No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG150250.11951562484836828TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGG36150.038.0904716
CCTCGGG37450.036.94597615
GGCGGTC47850.036.9140838
TCGGGGA48600.036.2955217
CTGACCC49800.035.24525540
CCAGGCG51150.034.31011635
GTCTCCC51850.033.9819642
CCCAGGC52350.033.7377334
CAGGCGG52950.033.3138736
CCTGACC53100.033.222839
ACGTCCG24100.033.04829832
CCAGGGA53300.033.02045
AGGCGGT54300.032.73423837
CGTCCGC24850.031.77924733
GCGGTCT55900.031.75995639
TAAGCCG55900.031.59592416
TGACCCT56550.031.19743341
AGTCACG26800.030.38920828
ACATCCC59200.030.21400534
CGGTCTC58800.030.00365340
Produced by FastQC (version 0.11.2)