FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l02n01 ah8335a-062415.3410000000e296.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l02n01 ah8335a-062415.3410000000e296.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15887398
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG4838353.0454011412063826TruSeq Adapter, Index 19 (97% over 40bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC253970.159856258400526No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA185600.11682215048681982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG588250.043.8553611
GATCGGA596000.043.677281
CACACGT593050.043.50419212
ACACGTC592400.043.4721813
ACGTCTG592500.043.3988215
CACGTCT593800.043.3227514
AGCACAC596600.043.23778510
AGTCACG583050.043.2014928
CGTCTGA595250.043.18281616
GAGCACA600050.043.067939
TCGGAAG605150.042.991513
GTCACGT585000.042.84596329
AGAGCAC605200.042.794378
TCACGTG587900.042.6384430
CACGTGA587050.042.60846731
CGATCTC580250.042.56593340
GAACTCC597450.042.55076221
GATCTCG587950.042.4983741
CAGTCAC596300.042.47168427
TCCAGTC596500.042.4191925