FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l02n01 ah8225b-062415.3410000000e253.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l02n01 ah8225b-062415.3410000000e253.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25736989
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA439362617.071251030957818No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC334906913.012668265118348No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG9852823.828272219411525TruSeq Adapter, Index 15 (97% over 40bp)
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC8595893.3398972972323997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGT3518200.044.5994761
TCCAGGG4629650.044.5263564
CCAGGGA4642850.044.4938055
CTTCCAG4639250.044.3956642
AGGGATT4657800.044.391997
TTCCAGG4642100.044.3889963
CAGGGAT4657500.044.374566
ACTTCCA4647900.044.3487171
GGGATTT4673500.044.2476778
GCACACG1195700.044.24028811
TCGTTGG3549300.044.179944
ATAAGCC4677750.044.1478915
TAAGCCG4676800.044.14765516
AGCCGAT4681100.044.08355718
CGTTGGA3566950.044.0805855
TATAAGC4694550.044.0780814
ATTCGTT3559450.044.074452
AAGCCGA4683500.044.06624617
TCGGAAG1214550.044.0600933
CCGATGA4680350.044.0103320
Produced by FastQC (version 0.11.2)