FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l02n01 ah8225a-062415.3410000000e26f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l02n01 ah8225a-062415.3410000000e26f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36937023
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC5660171.5323839173503506TruSeq Adapter, Index 4 (100% over 51bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC448180.12133625387189433No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA422610.1144136602454399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG706700.042.9864711
ACGTCTG718300.042.2528115
CACACGT720700.042.16703812
GATCGGA726400.042.160281
ACACGTC720800.042.12997413
CACGTCT722900.041.99033714
CGTCTGA728800.041.65642516
AGCACAC730450.041.58263410
GAGCACA736000.041.336329
ACTGACC706450.041.22075732
CAGTCAC723900.040.93762627
GTATGCC704500.040.88469345
TCCAGTC730050.040.7423925
GAACTCC740100.040.71197521
AGAGCAC748950.040.6846588
CCAGTCA731900.040.53873426
TCGGAAG755000.040.5254973
CGTATGC718000.040.06988544
AGTCACT735250.040.06699428
GTCTGAA758350.040.045117