FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l02n01 ah8148b-062415.3410000000e239.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l02n01 ah8148b-062415.3410000000e239.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29434284
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG27887789.474590922612556TruSeq Adapter, Index 13 (97% over 40bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA1537680.5224112127205133No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC1326810.45077026504194906No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC313120.10637935001238691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3328200.044.4415051
GCACACG3310200.044.17634611
TCGGAAG3344900.044.1032643
ATCGGAA3349350.044.086552
CACACGT3323950.044.00510812
ACACGTC3326950.043.9809813
GAGCACA3336300.043.9791079
AGCACAC3328250.043.97123710
CACGTCT3327750.043.91075514
ACGTCTG3327750.043.8937815
AGAGCAC3347200.043.8688248
CGTCTGA3338300.043.71416
CGGAAGA3375650.043.6575134
GTCTGAA3351200.043.53901317
TCGTATG3303050.043.4718345
GAACTCC3321800.043.4686821
TCTGAAC3355500.043.3618518
TCCAGTC3311200.043.28652225
ACTCCAG3323350.043.17091823
TGAACTC3354150.043.15793220
Produced by FastQC (version 0.11.2)