FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l02n01 ah8148a-062415.3410000000e246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l02n01 ah8148a-062415.3410000000e246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17502039
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG332154418.97804021577143TruSeq Adapter, Index 14 (97% over 44bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC200680.11466092607838435No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA188580.10774744588330537No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTCTG176490.10083967930822231TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTCTCTCGTATG175420.10022832196865748TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3845450.044.8018261
TCGGAAG3851100.044.579633
GCACACG3840100.044.52207611
ATCGGAA3862850.044.5123182
GAGCACA3841350.044.492369
AGAGCAC3847100.044.4662138
AGCACAC3842350.044.4585310
CACACGT3849200.044.41448612
ACACGTC3849700.044.3894313
CACGTCT3847150.044.33875314
CGGAAGA3865500.044.3204084
ACGTCTG3848000.044.31070715
CGTCTGA3843450.044.22604416
GAAGAGC3879750.044.144076
GGAAGAG3890350.044.100945
TCGTATG3780550.044.06815345
GTCTGAA3853250.044.04991517
TCTGAAC3851300.044.01846718
GAACTCC3830950.044.0161921
AAGAGCA3891750.043.9842537
Produced by FastQC (version 0.11.2)