FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l02n01 ah3337b-062415.3410000000e22c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l02n01 ah3337b-062415.3410000000e22c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10243676
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC178225817.39861744943905TruSeq Adapter, Index 5 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA1119121.0924984351320757No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC937440.9151402289568705No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC236020.230405569250726No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGCTCTCGTATGCC137080.13381914851660673TruSeq Adapter, Index 5 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2069350.044.8092271
GCACACG2056800.044.63186311
TCGGAAG2072550.044.6314163
ATCGGAA2076000.044.5902022
AGCACAC2059550.044.56244310
CACACGT2060050.044.5439812
GAGCACA2064100.044.5372359
CGGAAGA2074800.044.5222744
ACACGTC2060850.044.51577413
AGAGCAC2070300.044.4820988
CACGTCT2064650.044.44538514
ACGTCTG2064500.044.4355415
CGTCTGA2064800.044.33753216
GTCTGAA2067200.044.23489817
GAAGAGC2091500.044.174286
GAACTCC2051950.044.1649821
TCTGAAC2068750.044.10713218
GTATGCC2019250.044.08508745
GGAAGAG2102550.044.041695
TCCAGTC2048300.043.9880425
Produced by FastQC (version 0.11.2)