FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l02n01 ah3337a-062415.3410000000e21f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l02n01 ah3337a-062415.3410000000e21f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19874129
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG18839079.479192773680799TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTATG477640.24033254488787908TruSeq Adapter, Index 16 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTCTG236960.11923038237298347TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2340750.044.5497931
GCACACG2317300.044.47883611
AGCACAC2328900.044.3075310
GAGCACA2333900.044.2897269
TCGGAAG2352400.044.2258643
ACGTCTG2327650.044.19176515
CACGTCT2330350.044.18304414
AGAGCAC2346200.044.1591768
CACACGT2335700.044.13615812
CGTCTGA2336400.044.10692616
ACACGTC2336750.044.09321613
ATCGGAA2364100.044.0624242
CGTCCCG2228350.043.86641735
CGGAAGA2371950.043.8622974
GTCCCGA2148650.043.81298436
GTCTGAA2353450.043.8084217
GAACTCC2335800.043.77282721
CCCGTCC2260000.043.75197633
TCACCCG2288000.043.68363630
TCCAGTC2317050.043.68162525
Produced by FastQC (version 0.11.2)