##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_53115004-1.3420000003588a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4822136 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.181252457417212 33.0 33.0 33.0 22.0 33.0 2 29.422564191470336 33.0 33.0 33.0 15.0 33.0 3 29.14042511451357 33.0 33.0 33.0 15.0 33.0 4 32.36428420932135 37.0 37.0 37.0 15.0 37.0 5 32.516987077925634 37.0 37.0 37.0 15.0 37.0 6 33.31840723695889 37.0 37.0 37.0 15.0 37.0 7 32.53035210952159 37.0 37.0 37.0 15.0 37.0 8 32.89106134708768 37.0 33.0 37.0 15.0 37.0 9 32.07967340614201 37.0 33.0 37.0 6.0 37.0 10-11 32.19179436249828 37.0 33.0 37.0 15.0 37.0 12-13 32.154700634739456 37.0 33.0 37.0 10.5 37.0 14-15 33.57026657896003 37.0 33.0 40.0 15.0 40.0 16-17 33.47263225259512 37.0 33.0 40.0 10.5 40.0 18-19 33.55240032632842 37.0 33.0 40.0 10.5 40.0 20-21 33.58445748937815 37.0 33.0 40.0 10.5 40.0 22-23 34.66872263660751 37.0 33.0 40.0 18.5 40.0 24-25 33.792784152085304 37.0 33.0 40.0 15.0 40.0 26-27 33.54367981326118 37.0 33.0 40.0 10.5 40.0 28-29 33.19974250000415 37.0 33.0 40.0 6.0 40.0 30-31 33.28165205211964 37.0 33.0 40.0 10.5 40.0 32-33 32.989484017041406 37.0 33.0 40.0 6.0 40.0 34-35 33.10566116343463 37.0 33.0 40.0 10.5 40.0 36-37 32.91482363832127 37.0 33.0 40.0 6.0 40.0 38-39 33.19282647772688 37.0 33.0 40.0 10.5 40.0 40-41 32.73783526636329 37.0 33.0 40.0 6.0 40.0 42-43 32.53946715729295 37.0 33.0 40.0 6.0 40.0 44-45 32.44594698283085 37.0 33.0 40.0 6.0 40.0 46-47 32.39573064716549 37.0 33.0 40.0 6.0 40.0 48-49 32.32896697646022 37.0 33.0 40.0 6.0 40.0 50-51 31.48035310493109 37.0 30.0 38.5 10.5 38.5 52-53 31.882001876346912 37.0 33.0 38.5 10.5 40.0 54-55 32.48673056919175 37.0 33.0 40.0 6.0 40.0 56-57 32.877673504023946 37.0 33.0 40.0 10.5 40.0 58-59 32.214335721763135 37.0 33.0 40.0 6.0 40.0 60-61 32.025050828097754 37.0 33.0 40.0 6.0 40.0 62-63 31.620810051811063 37.0 33.0 40.0 6.0 40.0 64-65 31.448816147035256 37.0 33.0 37.0 6.0 40.0 66-67 31.350372220941093 37.0 33.0 37.0 6.0 40.0 68-69 31.56746968563309 37.0 33.0 37.0 10.5 40.0 70-71 31.155178327612497 37.0 30.0 37.0 10.5 40.0 72-73 31.638075637020606 37.0 27.0 37.0 15.0 37.0 74-75 31.90858376868674 37.0 27.0 37.0 18.5 37.0 76-77 31.57929743167758 37.0 27.0 37.0 22.0 37.0 78-79 31.00189304076036 33.0 27.0 37.0 15.0 37.0 80-81 30.32197536527381 33.0 27.0 37.0 15.0 37.0 82-83 29.93734571152701 33.0 27.0 37.0 6.0 37.0 84-85 29.631457408086376 33.0 27.0 37.0 6.0 37.0 86-87 29.4642219754897 33.0 27.0 37.0 6.0 37.0 88-89 29.346943657333597 33.0 27.0 37.0 6.0 37.0 90-91 29.214325352914145 33.0 27.0 37.0 6.0 37.0 92-93 29.043570732969787 33.0 27.0 37.0 6.0 37.0 94-95 28.906086327718672 33.0 27.0 37.0 6.0 37.0 96-97 28.76544460794967 33.0 27.0 37.0 6.0 37.0 98-99 28.62093966242346 33.0 27.0 37.0 6.0 37.0 100-101 27.487562047192363 33.0 21.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.020676428051174867 2306 2 0.10134008877267675 2306 3 0.11845890187300157 2306 4 0.324182135203543 2306 5 0.17419811224431214 2306 6 -0.28098005671814974 2306 7 0.4837327908653606 2306 8 -0.08912910550510134 2306 9 0.35438935200013333 2306 10-11 0.29343676098203986 2306 12-13 0.3104898079914946 2306 14-15 0.6116699746379908 2306 16-17 0.5168461679066993 2306 18-19 0.5808213632044712 2306 20-21 0.5810918296572183 2306 22-23 0.10944948393174059 2306 24-25 0.422621188972542 2306 26-27 -0.03623562798826896 2306 28-29 0.24871159936606801 2306 30-31 0.21799640373520646 2306 32-33 0.3773720574214465 2306 34-35 0.2390258213730263 2306 36-37 0.30903103137683985 2306 38-39 0.02767963558856934 2306 40-41 0.3087972382200732 2306 42-43 0.4107120780949174 2306 44-45 0.5279542201105656 2306 46-47 0.4135417675525943 2306 48-49 0.4743890918043263 2306 50-51 0.40621736161906696 2306 52-53 0.23864479740858258 2306 54-55 0.3979782790746782 2306 56-57 0.26431967510863785 2306 58-59 0.5594696727002386 2306 60-61 0.38980305194093035 2306 62-63 0.4238285051119668 2306 64-65 0.30725692841954455 2306 66-67 0.3664141034664432 2306 68-69 0.10228393090465815 2306 70-71 0.32537371489195266 2306 72-73 -0.12655460618609382 2306 74-75 -0.08531179036715741 2306 76-77 0.1730568796686427 2306 78-79 0.40771858915670833 2306 80-81 0.6537213141577567 2306 82-83 0.5956938699564027 2306 84-85 0.7455933516270647 2306 86-87 0.6979627544002973 2306 88-89 0.6042249791647052 2306 90-91 0.43803742186673134 2306 92-93 0.34814100188857466 2306 94-95 0.3748675065693341 2306 96-97 0.3623324091324669 2306 98-99 0.5003728192404111 2306 100-101 0.4773592033113694 2307 1 -0.2522775704216187 2307 2 -0.20468941363974125 2307 3 -0.27410894337545955 2307 4 -0.22323120418106868 2307 5 -0.293491352968978 2307 6 -0.6155171111454081 2307 7 0.02341398131284933 2307 8 -0.6343612344168434 2307 9 -0.1987402176127162 2307 10-11 -0.2116750328868804 2307 12-13 -0.2645525115061744 2307 14-15 -0.015855354157388035 2307 16-17 -0.011681456597152362 2307 18-19 -0.002797374850395329 2307 20-21 -0.12977767758390968 2307 22-23 -0.24824500317328813 2307 24-25 -0.021662104902169688 2307 26-27 -0.5481757433664498 2307 28-29 -0.29550145022080443 2307 30-31 -0.28085413999836106 2307 32-33 -0.14652009432015944 2307 34-35 -0.4016926455713019 2307 36-37 -0.20205675711453353 2307 38-39 -0.49343996826907954 2307 40-41 -0.2989569716075522 2307 42-43 -0.27003564379536726 2307 44-45 -0.19614428247232496 2307 46-47 -0.11144833991438219 2307 48-49 -0.16905174180142524 2307 50-51 -0.22477659693868546 2307 52-53 -0.3682980786436083 2307 54-55 -0.26382482978634414 2307 56-57 -0.44262622595246626 2307 58-59 0.03962061970236164 2307 60-61 -0.29127732669218176 2307 62-63 -0.04807123534980562 2307 64-65 -0.0383788459954566 2307 66-67 -0.09514307499749108 2307 68-69 -0.2008359087340601 2307 70-71 -0.21614439101144 2307 72-73 -0.626022590990182 2307 74-75 -0.4723089544919823 2307 76-77 -0.13822968300619465 2307 78-79 0.03214422619201329 2307 80-81 0.14282487397127852 2307 82-83 -0.11167251676657841 2307 84-85 0.01827363862408049 2307 86-87 0.09686559026604513 2307 88-89 -0.04858900003284461 2307 90-91 -0.07463430275304717 2307 92-93 -0.04115342562095137 2307 94-95 -0.07326499334420689 2307 96-97 -0.018703319992216194 2307 98-99 -0.08323343240697056 2307 100-101 -0.18047327508785216 2201 1 -0.08052886193450348 2201 2 -0.11532816888387387 2201 3 -0.20224645724622903 2201 4 -0.09732211156540416 2201 5 -0.16306651645612646 2201 6 -0.2528674009068794 2201 7 0.25938903806851954 2201 8 -0.3289795458330005 2201 9 0.07563573197133167 2201 10-11 -0.11936094792027774 2201 12-13 -0.029932160457057932 2201 14-15 0.32587877566891876 2201 16-17 0.12670218317152404 2201 18-19 0.2323917334397052 2201 20-21 0.08585919999297431 2201 22-23 0.1422850316008848 2201 24-25 0.21095768577175988 2201 26-27 -0.16298407243817792 2201 28-29 0.054751072231532305 2201 30-31 0.10850122858889932 2201 32-33 0.16830930339175865 2201 34-35 -0.0183278256950814 2201 36-37 0.03483796805059569 2201 38-39 -0.017591326847117728 2201 40-41 0.22856113471896577 2201 42-43 0.16566889965295672 2201 44-45 0.09965501659593201 2201 46-47 0.1436776567532263 2201 48-49 0.126526393298235 2201 50-51 0.09428984139851693 2201 52-53 0.019038612109529396 2201 54-55 0.2305376718409562 2201 56-57 0.17909236389628802 2201 58-59 0.21561920383386024 2201 60-61 0.22344099135103335 2201 62-63 0.24674491871791915 2201 64-65 0.17701211436170539 2201 66-67 0.07756594612624568 2201 68-69 -0.006367688459636867 2201 70-71 0.16243798724076441 2201 72-73 -0.22272655808503572 2201 74-75 -0.002475615032182077 2201 76-77 0.13247449067134554 2201 78-79 0.48839658746445025 2201 80-81 0.5033666287768348 2201 82-83 0.34936302829084553 2201 84-85 0.3720324787434848 2201 86-87 0.22286225787598468 2201 88-89 0.21435391617604793 2201 90-91 0.16392618087746058 2201 92-93 0.3806756763616548 2201 94-95 0.273333346782227 2201 96-97 0.321379065114062 2201 98-99 0.3141845110021215 2201 100-101 0.1466932390283553 1101 1 0.24125640507045887 1101 2 0.13870884904116565 1101 3 0.1600051344218656 1101 4 0.27845116178460216 1101 5 0.16536882807127284 1101 6 0.16380487127107557 1101 7 0.5851490285355894 1101 8 0.16411505848009966 1101 9 0.47606913505069315 1101 10-11 0.3574838535760918 1101 12-13 0.3673917486450833 1101 14-15 0.6092144740130223 1101 16-17 0.5242553834351753 1101 18-19 0.5474732447045625 1101 20-21 0.4336911585697081 1101 22-23 0.38704530468078957 1101 24-25 0.5437239315140516 1101 26-27 0.29213317306982134 1101 28-29 0.39765941114858805 1101 30-31 0.43847410742183257 1101 32-33 0.4240683423084306 1101 34-35 0.4476841568676946 1101 36-37 0.35260374673296724 1101 38-39 0.2099155786277933 1101 40-41 0.3677514865525495 1101 42-43 0.4008116603391443 1101 44-45 0.44991239869776933 1101 46-47 0.3938337989635059 1101 48-49 0.3534002523390001 1101 50-51 0.270141623598537 1101 52-53 0.4034534842797086 1101 54-55 0.613381138948327 1101 56-57 0.5863308972980477 1101 58-59 0.6188271567352004 1101 60-61 0.4628593904376075 1101 62-63 0.5290302670523168 1101 64-65 0.592730617706831 1101 66-67 0.38452311059163335 1101 68-69 0.3781616708316129 1101 70-71 0.42938962780159073 1101 72-73 0.1092130034902965 1101 74-75 0.24817721905489876 1101 76-77 0.550532572008219 1101 78-79 0.5250102818513369 1101 80-81 0.5084815092740698 1101 82-83 0.41523479780299866 1101 84-85 0.48990655669629746 1101 86-87 0.3883169852710573 1101 88-89 0.4670989726895023 1101 90-91 0.44228612632613107 1101 92-93 0.591506126322983 1101 94-95 0.5272142819193739 1101 96-97 0.44330804712864946 1101 98-99 0.4869782193920962 1101 100-101 0.4296867040717878 2304 1 0.0657644208717123 2304 2 -0.2081379339230942 2304 3 -0.18480632607099068 2304 4 -0.19777826868037351 2304 5 -0.13734305862452345 2304 6 0.04881144366655832 2304 7 0.19650264117566962 2304 8 -0.03916205709206366 2304 9 0.039222363546578265 2304 10-11 0.0051287857835760065 2304 12-13 0.012185932963241441 2304 14-15 0.10089551600415092 2304 16-17 0.0250527949313053 2304 18-19 0.07318910291501624 2304 20-21 0.07670035304729339 2304 22-23 0.07731278742466685 2304 24-25 0.005078983835360873 2304 26-27 0.014926242028984404 2304 28-29 -0.012386333468860755 2304 30-31 0.06531800959111678 2304 32-33 0.031690315539812275 2304 34-35 -0.008915112662400304 2304 36-37 0.060927625443333966 2304 38-39 0.016790507718120296 2304 40-41 0.13331558660423326 2304 42-43 0.0774223699630312 2304 44-45 0.04122160182776469 2304 46-47 -0.041263938917673215 2304 48-49 -0.009766714010567057 2304 50-51 0.04575251400627778 2304 52-53 0.024454787585156623 2304 54-55 0.2153176964227015 2304 56-57 0.3611045521917049 2304 58-59 0.1697803761202792 2304 60-61 0.05625326630585903 2304 62-63 0.1351106353585827 2304 64-65 0.2458048313400525 2304 66-67 0.30367333480944936 2304 68-69 0.2554087553696931 2304 70-71 0.260493460763044 2304 72-73 0.35760299580054067 2304 74-75 0.5688960440114421 2304 76-77 0.4889063725603755 2304 78-79 0.40239918573391975 2304 80-81 0.2734150146242378 2304 82-83 0.22554479913237202 2304 84-85 0.27024046103155897 2304 86-87 0.1315542438773427 2304 88-89 0.12308943612647383 2304 90-91 0.08087936724481892 2304 92-93 0.15904142931213272 2304 94-95 0.02048559865611921 2304 96-97 -0.0013018651553693417 2304 98-99 0.14084389956913057 2304 100-101 0.31349751735272946 2202 1 -0.07395874952451464 2202 2 -0.34702996383513707 2202 3 -0.27605050866715075 2202 4 -0.3448961874690539 2202 5 -0.38840975438035485 2202 6 -0.1871868624991464 2202 7 -0.10422152707853627 2202 8 -0.19584211610838764 2202 9 -0.3067883037973438 2202 10-11 -0.24330195117858722 2202 12-13 -0.27274057739809265 2202 14-15 -0.24696903829796923 2202 16-17 -0.21142119379089053 2202 18-19 -0.179637946712063 2202 20-21 -0.13650563018539685 2202 22-23 -0.07398472416377189 2202 24-25 -0.16632298988341887 2202 26-27 -0.16865357862446473 2202 28-29 -0.29165459112743264 2202 30-31 -0.16831151077764872 2202 32-33 -0.2922676003021749 2202 34-35 -0.24857875673465202 2202 36-37 -0.2246857816054515 2202 38-39 -0.275659475745357 2202 40-41 -0.23373406880764236 2202 42-43 -0.289330812930892 2202 44-45 -0.24328908071327504 2202 46-47 -0.20383803128934375 2202 48-49 -0.11497607872997406 2202 50-51 -0.16397896457506533 2202 52-53 -0.28412142697337117 2202 54-55 -0.10933983403077718 2202 56-57 0.038618663613853244 2202 58-59 -0.13974907099325407 2202 60-61 -0.2004383901692961 2202 62-63 -0.033460328468162714 2202 64-65 -0.09996107231127382 2202 66-67 0.013155381093159946 2202 68-69 0.016654523359729723 2202 70-71 0.11599524114362225 2202 72-73 0.15821466677219576 2202 74-75 0.39098751595756553 2202 76-77 0.232308152580341 2202 78-79 0.0954390168553445 2202 80-81 -0.0062834308397548 2202 82-83 -0.026625200682172334 2202 84-85 -0.054224336527425976 2202 86-87 -0.0029956935826689346 2202 88-89 -0.03958865344686657 2202 90-91 -0.09718914907378817 2202 92-93 -0.10029854971070051 2202 94-95 -0.1388166065224432 2202 96-97 -0.07036808104299652 2202 98-99 -0.12543427047164002 2202 100-101 0.0233300953808957 1102 1 -0.04819498414241963 1102 2 -0.31415306310658764 1102 3 -0.25113116595521134 1102 4 -0.19615789943229345 1102 5 -0.2739303623281586 1102 6 0.054380224362482465 1102 7 -0.13463078702312714 1102 8 0.22337969906651267 1102 9 -0.17137976147220613 1102 10-11 -0.19966580906653775 1102 12-13 -0.15633228702444057 1102 14-15 -0.2606158205807887 1102 16-17 -0.20348326687507523 1102 18-19 -0.2070689004497197 1102 20-21 -0.22682995137873974 1102 22-23 -0.015211890525655747 1102 24-25 -0.02382679269585708 1102 26-27 0.05868472658950452 1102 28-29 -0.10863455171115532 1102 30-31 0.0032058449130758504 1102 32-33 -0.12219609801221054 1102 34-35 -0.02385569649713659 1102 36-37 -0.03889698656983853 1102 38-39 -0.004385682665713375 1102 40-41 -0.14510678209112626 1102 42-43 -0.11547296207417901 1102 44-45 0.05364381658895212 1102 46-47 -0.054268103624814046 1102 48-49 0.05817279834538169 1102 50-51 0.001257751404285301 1102 52-53 0.057038257479511856 1102 54-55 0.14848557072514268 1102 56-57 0.20309079387077134 1102 58-59 0.06347468104083731 1102 60-61 0.11411727213354084 1102 62-63 0.16931450480301535 1102 64-65 0.03750753718761146 1102 66-67 0.20902551679600023 1102 68-69 0.14322259320307396 1102 70-71 0.1982463006666606 1102 72-73 0.320718065834523 1102 74-75 0.42421686342605724 1102 76-77 0.2526226040094919 1102 78-79 0.3126401411924036 1102 80-81 0.1191777644945482 1102 82-83 0.09052504828755303 1102 84-85 0.2110720146273124 1102 86-87 0.1093150895698507 1102 88-89 0.17750158941096927 1102 90-91 0.10138442889121535 1102 92-93 0.04358928708180798 1102 94-95 0.1228223678324376 1102 96-97 0.048943671965798075 1102 98-99 0.17811196804680662 1102 100-101 0.08201170717416417 2305 1 0.041671311373420394 2305 2 -0.2063588450946625 2305 3 -0.17225230964560723 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0.16050083930167602 1213 66-67 0.12772607146598958 1213 68-69 0.19647284507753326 1213 70-71 0.20311951475280665 1213 72-73 0.5380579522452393 1213 74-75 0.32789149960505526 1213 76-77 0.08916410510563821 1213 78-79 0.05456297475695493 1213 80-81 -0.016281789803198166 1213 82-83 0.10756562633418199 1213 84-85 0.11189977619232394 1213 86-87 0.23268004830781663 1213 88-89 0.13287834234376206 1213 90-91 0.1497858348506611 1213 92-93 0.09387880616334598 1213 94-95 0.25285723112279257 1213 96-97 0.20658305160257484 1213 98-99 0.03795750745307913 1213 100-101 0.20907373761421155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2331.0 3 918.0 4 996.0 5 700.0 6 1713.0 7 10757.0 8 66388.0 9 176465.0 10 190747.0 11 110884.0 12 57203.0 13 34558.0 14 23206.0 15 17917.0 16 15586.0 17 14549.0 18 14647.0 19 15179.0 20 16282.0 21 17454.0 22 19264.0 23 21382.0 24 23745.0 25 27320.0 26 32771.0 27 39896.0 28 48728.0 29 59923.0 30 71577.0 31 91666.0 32 125978.0 33 179546.0 34 272623.0 35 458683.0 36 880590.0 37 1353006.0 38 326856.0 39 102.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94807228837978 22.322210225510673 22.37782819123678 13.351889294872768 2 31.44371004149602 27.125087987988927 29.66402884654375 11.767173123971308 3 35.23999473628737 21.62618784344322 28.434317826377725 14.699499593891682 4 26.425713446537475 18.336642875241875 34.27586509374564 20.96177858447501 5 36.062951140276404 16.293475974246807 29.677141339062917 17.96643154641387 6 33.359997190945286 16.39156145004568 34.94042289986281 15.308018459146226 7 37.3350198455969 18.919302880571255 27.229446670280623 16.51623060355123 8 26.571462929797455 33.3119963313095 23.989318760479414 16.127221978413626 9 33.820663557338285 27.65389815381811 21.975916513681646 16.54952177516196 10-11 27.672326458971046 31.459563280082225 25.6020763540976 15.266033906849128 12-13 30.93314895695411 23.976100568330015 25.65499784861921 19.435752626096665 14-15 27.036441180068156 24.10252091800615 27.67043468708083 21.190603214844863 16-17 29.097780411772572 25.055495935351026 28.4684388541735 17.3782847987029 18-19 29.294659853097997 25.331656932997138 28.78857340283107 16.585109811073792 20-21 27.43244963298373 28.11392817980236 27.729033996892444 16.72458819032147 22-23 31.37551834685648 25.05523321324683 26.83906297219802 16.730185467698668 24-25 26.111905350322058 30.234298829287926 26.66307955565705 16.990716264732963 26-27 22.2887961156473 33.97607350966022 26.523894016818687 17.21123635787379 28-29 24.3494648175871 31.536045727630956 26.273202232775 17.84128722200694 30-31 28.67272499059139 27.236241459846212 26.425171879385505 17.66586167017689 32-33 24.903392326394336 26.4616292670383 28.610320408342442 20.024657998224924 34-35 19.78917667190063 26.39268830641499 35.42278843209877 18.39534658958561 36-37 21.542247868642907 26.994107222584457 29.704474840852985 21.759170067919648 38-39 19.99817319067853 26.357529471344687 26.25751270131834 27.386784636658447 40-41 22.157339860386344 25.844183636515528 28.009226290579463 23.989250212518662 42-43 25.565223344585636 26.00979412485688 30.3821489973851 18.04283353317238 44-45 22.702312911413053 28.424828038472445 31.29723727663622 17.575621773478282 46-47 24.948860488916615 29.97368422147872 27.636010289914726 17.441444999689935 48-49 20.57164611228143 29.314014885139215 30.519009281857006 19.595329720722344 50-51 19.117636347858696 26.673098305223007 32.29078636561992 21.91847898129837 52-53 26.35873166803817 26.181375756711965 27.17194930250897 20.28794327274089 54-55 29.472829234279036 26.214734084456463 26.466911562392497 17.845525118872 56-57 30.023778046047177 26.01586229540123 25.941925222747525 18.01843443580406 58-59 27.409688436757772 25.99282800357518 26.509360711973358 20.088122847693693 60-61 21.96742790527836 26.42071694468414 25.80156987458247 25.81028527545503 62-63 24.91321051223892 26.560172588997183 26.4528348112989 22.073782087465002 64-65 21.630758142792736 28.360635875451944 30.144720893191707 19.863885088563613 66-67 19.860175196548994 28.162539442939217 28.56428117323605 23.41300418727574 68-69 19.294565006333254 26.49975034105149 35.16251544312981 19.043169209485438 70-71 18.63997174991437 32.28110914860639 31.213849030494245 17.865070070984995 72-73 18.344282610083535 38.115648568663936 25.883624414055472 17.656444407197057 74-75 18.9710034252753 38.139143426450154 25.1150500535183 17.774803094756248 76-77 19.29014839079549 37.22064566142877 25.465685005440854 18.02352094233489 78-79 18.931859845337833 36.55681708292217 26.38501875307395 18.126304318666044 80-81 18.257578562185245 36.49451488341969 26.25359237387227 18.994314180522792 82-83 17.87648558293245 35.57850660709235 26.635276290679403 19.909731519295793 84-85 18.21161684605968 34.547995128359005 26.934371174529346 20.306016851051968 86-87 18.433257705594325 34.02790619886053 27.744310290205416 19.79452580533973 88-89 18.9500281149971 34.05502093490739 27.359792704163453 19.635158245932065 90-91 19.360750996047635 33.26039332687867 26.979773059125506 20.399082617948192 92-93 19.05547011240021 33.70551539900567 26.71818213014466 20.52083235844946 94-95 18.680168810418113 34.07291007890034 26.62248560925084 20.624435501430703 96-97 19.280482581990867 34.33503430686039 26.31344616349106 20.071036947657674 98-99 19.94644323125006 32.99770731015778 26.145286620553538 20.91056283803862 100-101 20.061948518035642 33.1244217678938 25.56883477812269 21.244794935947876 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1307.0 1 1042.0 2 592.0 3 556.0 4 663.5 5 622.0 6 752.5 7 993.0 8 1229.0 9 1509.0 10 1710.0 11 1744.5 12 1729.0 13 1619.5 14 1443.5 15 1328.0 16 1285.0 17 1275.0 18 1320.5 19 1457.0 20 1681.5 21 2016.0 22 2456.5 23 3205.0 24 4299.0 25 5594.5 26 7358.5 27 9947.5 28 13034.0 29 16443.5 30 20499.5 31 25923.0 32 33787.5 33 48871.0 34 74516.5 35 116843.5 36 170863.0 37 232992.0 38 301892.0 39 338708.0 40 336248.5 41 303352.5 42 269547.0 43 273038.5 44 269342.0 45 251514.0 46 252012.0 47 233908.5 48 187828.0 49 150753.0 50 127973.0 51 114045.0 52 102260.5 53 89873.0 54 77885.5 55 65008.0 56 53169.5 57 43442.5 58 35515.5 59 28670.0 60 22854.0 61 18217.0 62 14288.0 63 10946.0 64 8499.5 65 6531.0 66 4990.0 67 3844.5 68 2911.5 69 2200.5 70 1644.0 71 1259.5 72 987.0 73 741.0 74 552.0 75 416.5 76 320.0 77 239.0 78 182.0 79 145.5 80 107.0 81 87.5 82 74.5 83 61.5 84 48.0 85 39.0 86 27.5 87 18.5 88 15.5 89 11.5 90 8.5 91 6.0 92 4.5 93 4.0 94 3.5 95 2.5 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2973163759794415 2 0.2745878589902898 3 0.2455965572103317 4 0.23431524950768703 5 0.2394789363054049 6 0.18931444488500532 7 0.15482765313960453 8 0.1521939654957886 9 0.13075118578157066 10-11 0.11647328072041103 12-13 0.1373665114380847 14-15 0.14074675620928154 16-17 0.1341314305527675 18-19 0.1216058609711547 20-21 0.1272672525204598 22-23 0.15659035746814273 24-25 0.5638787458503867 26-27 0.21823316472202361 28-29 0.19267395195821935 30-31 0.2911676485275405 32-33 0.3231348099680308 34-35 0.33235271672138655 36-37 0.32296890838416836 38-39 0.3306003812418397 40-41 0.33361771629833753 42-43 0.3442872619104895 44-45 0.32522931746429384 46-47 0.3453656222055952 48-49 0.3415602546257509 50-51 0.34683799876237414 52-53 0.331409151463169 54-55 0.3235184573807126 56-57 0.3322593970804639 58-59 0.3248353012026206 60-61 0.30182682529070104 62-63 0.28929088686009685 64-65 0.2993175638347819 66-67 0.31504710775473777 68-69 0.30230379234430554 70-71 0.28317326595517006 72-73 0.27885982477474713 74-75 0.2702847866588582 76-77 0.2725970399839407 78-79 0.2818460532842707 80-81 0.2910328534906523 82-83 0.31402059170458896 84-85 0.3052796520048377 86-87 0.30727047101118676 88-89 0.31299407565444026 90-91 0.30609879107515836 92-93 0.2876526087194554 94-95 0.2833806429349981 96-97 0.30063440765668986 98-99 0.3090020687927508 100-101 0.3093753473564412 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4822136.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.006205296697686 #Duplication Level Percentage of deduplicated Percentage of total 1 91.69453547152214 44.01906694431219 2 3.6830212777976974 3.5361575114812425 3 1.2813172173756504 1.8453353216258666 4 0.7875373147430096 1.5122671204145173 5 0.39189840674352927 0.94067776847893 6 0.32689875666549784 0.9415901294231465 7 0.23856120882240375 0.8016692856589677 8 0.16094287672200866 0.6181005424766284 9 0.13566216667763092 0.5861363242069053 >10 0.9866612245034496 9.671761755360967 >50 0.1433953303636439 4.872996912476782 >100 0.13565532761105137 13.482538475476685 >500 0.022521540396927723 7.530049930950746 >1k 0.011087101193862395 8.458090498573675 >5k 2.1770006898765964E-4 0.7234751956544285 >10k+ 8.707879273762332E-5 0.46008628342835467 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 11835 0.24543065562646926 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 10177 0.21104755237098247 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 9278 0.1924043618844429 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7787 0.16148445419208418 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6420 0.13313602104959296 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6127 0.1270598755406318 No Hit TTGTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA 5001 0.10370922761199601 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 4935 0.10234053954513103 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0067397518444108585 0.0 0.0 2 0.007175243502049714 0.0 0.0 3 0.007755899045568188 0.0 0.0 4 0.008336554589086663 0.0 0.0 5 0.009145324810415965 0.0 0.0 6 0.009912619635779663 0.0 0.0 7 0.011053193024833808 0.0 0.0 8 0.012214504111870757 0.0 0.0 9 0.013375815198907704 0.0 0.0 10-11 0.01984597696954213 0.0 0.0 12-13 0.02418015584794788 0.0 0.0 14-15 0.0290327771759237 0.0 2.073769798280264E-5 16-17 0.03293146439669059 0.0 2.073769798280264E-5 18-19 0.03737970061400176 0.0 3.1106546974203964E-5 20-21 0.04230490388491739 0.0 4.147539596560528E-5 22-23 0.04747895953162665 0.0 4.147539596560528E-5 24-25 0.05342031000369961 0.0 4.147539596560528E-5 26-27 0.059651988247531804 0.0 4.147539596560528E-5 28-29 0.06652653512883087 0.0 4.147539596560528E-5 30-31 0.10319078516242594 0.0 4.147539596560528E-5 32-33 0.1121805772379709 0.0 5.1844244957006604E-5 34-35 0.11951135347489161 0.0 6.221309394840793E-5 36-37 0.12745389180230504 0.0 6.221309394840793E-5 38-39 0.1358630283343315 0.0 6.221309394840793E-5 40-41 0.14595191840296498 0.0 9.331964092261188E-5 42-43 0.15616523465949528 0.0 1.0368848991401321E-4 44-45 0.16693846876156127 0.0 1.0368848991401321E-4 46-47 0.1783442026521027 0.0 1.2442618789681585E-4 48-49 0.19112899345850054 0.0 1.2442618789681585E-4 50-51 0.2048573495231159 0.0 1.2442618789681585E-4 52-53 0.21878271371856786 0.0 1.2442618789681585E-4 54-55 0.23357906122929756 0.0 1.451638858796185E-4 56-57 0.2496818837129438 0.0 1.451638858796185E-4 58-59 0.2674126154882401 0.0 1.451638858796185E-4 60-61 0.2857965847499946 0.0 1.451638858796185E-4 62-63 0.30595362718927877 0.0 1.451638858796185E-4 64-65 0.32750009539341074 0.0 1.555327348710198E-4 66-67 0.3514521365635478 0.0 1.6590158386242112E-4 68-69 0.3761922102570313 0.0 1.6590158386242112E-4 70-71 0.40393925015802123 0.0 1.8663928184522378E-4 72-73 0.43371858446132583 0.0 1.8663928184522378E-4 74-75 0.4666500488580164 0.0 2.0737697982802641E-4 76-77 0.5036461020593364 0.0 2.1774582881942773E-4 78-79 0.5436283837701799 0.0 2.2811467781082905E-4 80-81 0.5888676719196638 0.0 2.2811467781082905E-4 82-83 0.6400275728432379 0.0 2.2811467781082905E-4 84-85 0.6979479633092056 0.0 2.3848352680223036E-4 86-87 0.7609801963279343 0.0 2.488523757936317E-4 88-89 0.8311669351507298 0.0 2.488523757936317E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 8950 0.0 88.00882 2 GAGCGGC 5785 0.0 86.96714 9 CGGGAGA 8325 0.0 85.35471 4 AGAGCGG 6310 0.0 84.39576 8 CGGAAAG 4365 0.0 83.95045 5 GTTCGGA 860 0.0 81.70828 2 ATTCGGA 1095 0.0 81.51669 2 TCGGAGA 1655 0.0 81.166794 4 TCGGGAG 7995 0.0 80.74655 3 AAGCGGC 2645 0.0 80.67334 9 GGATCGG 2785 0.0 80.30521 1 GAGAGCG 7305 0.0 80.04195 7 ACCGGAA 1555 0.0 77.84187 3 ATCCGGA 1565 0.0 77.36218 2 GAACCGG 1255 0.0 76.806915 1 CGGAGAG 3405 0.0 75.27774 5 AACCGGA 1235 0.0 74.967064 2 CCGGAAA 2350 0.0 74.532974 4 GATCGGG 10925 0.0 74.36637 1 TCGGGAA 3340 0.0 73.7606 3 >>END_MODULE