FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_53115004-1.3420000003588a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_53115004-1.3420000003588a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4822136
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT118350.24543065562646926Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC101770.21104755237098247No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG92780.1924043618844429No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA77870.16148445419208418No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC64200.13313602104959296No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG61270.1270598755406318No Hit
TTGTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTA50010.10370922761199601No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA49350.10234053954513103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA89500.088.008822
GAGCGGC57850.086.967149
CGGGAGA83250.085.354714
AGAGCGG63100.084.395768
CGGAAAG43650.083.950455
GTTCGGA8600.081.708282
ATTCGGA10950.081.516692
TCGGAGA16550.081.1667944
TCGGGAG79950.080.746553
AAGCGGC26450.080.673349
GGATCGG27850.080.305211
GAGAGCG73050.080.041957
ACCGGAA15550.077.841873
ATCCGGA15650.077.362182
GAACCGG12550.076.8069151
CGGAGAG34050.075.277745
AACCGGA12350.074.9670642
CCGGAAA23500.074.5329744
GATCGGG109250.074.366371
TCGGGAA33400.073.76063
Produced by FastQC (version 0.11.2)