FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_52370180-2.342000000358cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_52370180-2.342000000358cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5395423
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC95170.17639024780818854No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC88110.163305082845219No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG83830.1553724332642686No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG71560.13263093551701138No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA68380.1267370510152772No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA55380.1026425546245401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC47800.087.472399
CGGGAGA66650.084.516344
AGAGCGG50900.084.287888
TCGGGAG70950.081.941153
CGGAAAG33850.080.752115
AAGCGGC17850.080.470149
ATTCGGA13550.079.880922
TCGGAGA20100.079.565344
GTTCGGA12650.079.559642
GAGAGCG64100.078.98927
CGGAGAG34550.077.605155
ATCCGGA14050.077.376072
CCGGAGA15700.073.7531054
TTCGGAG15200.073.68633
ATCGGGA89400.073.599222
GGATCGG31500.073.406511
GTCCGGA7700.072.750972
GATCGGG95550.069.916471
TCCGGAG13750.069.376393
GAACCGG7700.069.062681
Produced by FastQC (version 0.11.2)