FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_52260024-1.3420000003582d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_52260024-1.3420000003582d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6664533
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA78360.11757763072071216No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC72880.10935499906745154No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG70570.10588888973916102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC20150.074.354419
CGGGAGA29350.073.567034
CGGAAAG14950.068.8808445
AGAGCGG23250.067.4998868
ATTCGGA5050.062.0345732
ACCGGAA5850.061.6520423
AAGCGGC10200.059.963129
GATCCGG7550.059.1089971
GAACCGG5100.058.646631
GAGAGCG29900.057.247117
TCGGGAA12100.056.0841873
TCGGGAG34950.055.8064463
AACCGGA5450.055.7397042
GGTCCGG5500.050.065431
CGGAGAG18850.049.091155
TTGTGTT693750.048.9574361
TCGGAGA9850.047.6956634
TCGGAAA13500.046.4002234
GTTCGGA4600.045.402112
GGATCGG16500.043.735321
Produced by FastQC (version 0.11.2)