##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_52150223-1.3420000003573b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3271564 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.498661190794373 33.0 33.0 33.0 27.0 33.0 2 31.233195193491554 33.0 33.0 33.0 27.0 33.0 3 31.147376912082418 33.0 33.0 33.0 27.0 33.0 4 34.71092786202563 37.0 37.0 37.0 33.0 37.0 5 34.760734621116995 37.0 37.0 37.0 33.0 37.0 6 35.02405241040677 37.0 37.0 37.0 33.0 37.0 7 34.75361784149722 37.0 37.0 37.0 33.0 37.0 8 34.830423919568744 37.0 37.0 37.0 33.0 37.0 9 34.53283719957794 37.0 37.0 37.0 33.0 37.0 10-11 34.53784031123952 37.0 37.0 37.0 33.0 37.0 12-13 34.497852708979565 37.0 37.0 37.0 33.0 37.0 14-15 36.11905192745733 40.0 37.0 40.0 27.0 40.0 16-17 36.07769326841841 40.0 37.0 40.0 27.0 40.0 18-19 36.06659032193777 40.0 37.0 40.0 27.0 40.0 20-21 36.04304225746462 40.0 37.0 40.0 27.0 40.0 22-23 36.36952891644486 40.0 37.0 40.0 27.0 40.0 24-25 35.91006808975768 40.0 37.0 40.0 27.0 40.0 26-27 35.820195019874284 40.0 37.0 40.0 27.0 40.0 28-29 35.66611397484506 40.0 37.0 40.0 27.0 40.0 30-31 35.655606309398195 40.0 37.0 40.0 27.0 40.0 32-33 35.539493190412905 40.0 37.0 40.0 27.0 40.0 34-35 35.49526999930309 38.5 37.0 40.0 27.0 40.0 36-37 35.39667449574577 37.0 37.0 40.0 27.0 40.0 38-39 35.26800453850207 37.0 37.0 40.0 27.0 40.0 40-41 35.232362717036864 37.0 37.0 40.0 24.5 40.0 42-43 35.11940023181573 37.0 37.0 40.0 22.0 40.0 44-45 34.97916501098557 37.0 37.0 40.0 22.0 40.0 46-47 34.85521787133004 37.0 37.0 40.0 22.0 40.0 48-49 34.7451191540193 37.0 37.0 40.0 22.0 40.0 50-51 33.76173475438659 37.0 33.0 38.5 22.0 38.5 52-53 34.178437285652976 37.0 35.0 38.5 22.0 40.0 54-55 34.857385947516235 37.0 37.0 40.0 22.0 40.0 56-57 35.041063998748 37.0 37.0 40.0 22.0 40.0 58-59 34.73321154652638 37.0 37.0 40.0 22.0 40.0 60-61 34.564830154629405 37.0 33.0 40.0 22.0 40.0 62-63 34.302495381413905 37.0 33.0 40.0 22.0 40.0 64-65 34.13326378453853 37.0 33.0 40.0 22.0 40.0 66-67 33.92248615646828 37.0 33.0 40.0 22.0 40.0 68-69 33.850083935389925 37.0 33.0 40.0 22.0 40.0 70-71 33.53153491724447 37.0 33.0 37.0 22.0 40.0 72-73 33.522407325670535 37.0 33.0 37.0 22.0 40.0 74-75 33.442764378138406 37.0 33.0 37.0 22.0 40.0 76-77 33.148851741858024 37.0 33.0 37.0 22.0 40.0 78-79 32.76968737276728 37.0 33.0 37.0 22.0 37.0 80-81 32.32992492275866 37.0 33.0 37.0 22.0 37.0 82-83 32.00006220266515 37.0 33.0 37.0 15.0 37.0 84-85 31.685846280250058 37.0 33.0 37.0 15.0 37.0 86-87 31.50444435749996 33.0 33.0 37.0 15.0 37.0 88-89 31.33525861025491 33.0 33.0 37.0 15.0 37.0 90-91 31.155591331852285 33.0 33.0 37.0 15.0 37.0 92-93 30.97445762943962 33.0 33.0 37.0 15.0 37.0 94-95 30.807070716024505 33.0 30.0 37.0 15.0 37.0 96-97 30.65454855842649 33.0 27.0 37.0 15.0 37.0 98-99 30.489877471447905 33.0 27.0 37.0 15.0 37.0 100-101 29.216360737555494 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.033309577678203084 2306 2 0.10379077863657216 2306 3 0.0331282702646547 2306 4 0.15826347709936073 2306 5 0.11713913498293493 2306 6 -0.10850075868862774 2306 7 0.10036276878327755 2306 8 0.053292824904154656 2306 9 0.15215639805667536 2306 10-11 0.11780803747907953 2306 12-13 0.17802222630211162 2306 14-15 0.31844324568367455 2306 16-17 0.31971235792853747 2306 18-19 0.3005281300733955 2306 20-21 0.2947100390286792 2306 22-23 0.07603677374191875 2306 24-25 0.3103947004425862 2306 26-27 0.08861719654422018 2306 28-29 0.14874021437339735 2306 30-31 0.13180926300567108 2306 32-33 0.20861188390613705 2306 34-35 0.06915645110272806 2306 36-37 -0.040305052135018116 2306 38-39 -0.030790290275398036 2306 40-41 0.04578598045110738 2306 42-43 0.19017554366084255 2306 44-45 0.13517683509038392 2306 46-47 0.11985501704047152 2306 48-49 0.08667533572278785 2306 50-51 0.0762864177703122 2306 52-53 0.09643868012130241 2306 54-55 0.19561443727022976 2306 56-57 0.09007433002575027 2306 58-59 0.2602163917537652 2306 60-61 0.15795927829393008 2306 62-63 0.29581379364042704 2306 64-65 0.15106630417552225 2306 66-67 0.26859842493733765 2306 68-69 0.012886944678065504 2306 70-71 0.28333432079909215 2306 72-73 0.024910328884310218 2306 74-75 0.002970446138270688 2306 76-77 0.08284979523464386 2306 78-79 0.09520963201978816 2306 80-81 0.21805652740938086 2306 82-83 0.11264602782146937 2306 84-85 0.22405946780929042 2306 86-87 0.31639668395722254 2306 88-89 0.2665628741769588 2306 90-91 0.10218014519538698 2306 92-93 0.15538107350032604 2306 94-95 -0.1044808684033427 2306 96-97 -0.011470670338670885 2306 98-99 0.052254611334621615 2306 100-101 0.0934755854967797 2307 1 0.07763220222404854 2307 2 0.1080453930985037 2307 3 0.05091565591014202 2307 4 0.13424030184827984 2307 5 0.0738265242089966 2307 6 0.11407474661957195 2307 7 0.1910521631295552 2307 8 0.05660194462057433 2307 9 0.27636285382766346 2307 10-11 0.22976065756250819 2307 12-13 0.15876971542612495 2307 14-15 0.3336901037319038 2307 16-17 0.31621973390648606 2307 18-19 0.33520608001699514 2307 20-21 0.32206314606987263 2307 22-23 0.2020706638541725 2307 24-25 0.2766902184618161 2307 26-27 0.13374717130118796 2307 28-29 0.07844676413439089 2307 30-31 0.0845250148362382 2307 32-33 0.15422534775131425 2307 34-35 0.1279949807409153 2307 36-37 0.09182990856820084 2307 38-39 0.04763217691760957 2307 40-41 0.2577155506034643 2307 42-43 0.22525216789772884 2307 44-45 0.2307214848209398 2307 46-47 0.0884575422387428 2307 48-49 0.14243781849128823 2307 50-51 -0.047615129931855904 2307 52-53 -0.007435722370779274 2307 54-55 -0.022957876853340053 2307 56-57 -0.15634140569224542 2307 58-59 0.10526110414433276 2307 60-61 0.11738490981728233 2307 62-63 0.03981823802519813 2307 64-65 -0.03553609122702284 2307 66-67 -0.009014833024956204 2307 68-69 -0.03190757525676702 2307 70-71 0.003294889751948915 2307 72-73 -0.11948484540001658 2307 74-75 -0.09717568594902559 2307 76-77 -0.004487736319148894 2307 78-79 0.008896815362973598 2307 80-81 0.024410252099208662 2307 82-83 0.12752901025876184 2307 84-85 0.13289273848262084 2307 86-87 0.22196592774045243 2307 88-89 -0.13460853227164904 2307 90-91 -0.005250324575634124 2307 92-93 -0.04029963795929348 2307 94-95 -0.04494972190424562 2307 96-97 0.04946987874201625 2307 98-99 0.03135235199380304 2307 100-101 -0.04287628177142011 2201 1 0.1029714106074806 2201 2 0.10512986282880021 2201 3 0.10967837622452237 2201 4 0.183562984820405 2201 5 0.08258350144195248 2201 6 0.09457270843662968 2201 7 0.3436085675206897 2201 8 0.012329507501206649 2201 9 0.22800366440861808 2201 10-11 0.19558626828423087 2201 12-13 0.24901368641256738 2201 14-15 0.5466426463284577 2201 16-17 0.3972063464171143 2201 18-19 0.4640646364751504 2201 20-21 0.34949735723930786 2201 22-23 0.36185174247817997 2201 24-25 0.42714703782112906 2201 26-27 0.2747373762925349 2201 28-29 0.29532537494409183 2201 30-31 0.3672449992932627 2201 32-33 0.29404097039908805 2201 34-35 0.43325717073670944 2201 36-37 0.2905936797442834 2201 38-39 0.3050239503811554 2201 40-41 0.21194930134884515 2201 42-43 0.3374805323658947 2201 44-45 0.32411681899496614 2201 46-47 0.25656333957395105 2201 48-49 0.38456002148844703 2201 50-51 -0.01919084818360517 2201 52-53 0.16544141478866692 2201 54-55 0.3396220986102634 2201 56-57 0.35920248855686054 2201 58-59 0.42111530807993347 2201 60-61 0.30184840825809545 2201 62-63 0.3248226247618575 2201 64-65 0.20232767799397067 2201 66-67 0.3202723772549092 2201 68-69 0.21754927587278416 2201 70-71 0.1847231085657981 2201 72-73 0.07513016041637854 2201 74-75 0.1053333074720868 2201 76-77 0.17812086901628987 2201 78-79 0.30816634428366285 2201 80-81 0.45594783384647286 2201 82-83 0.4391757960882572 2201 84-85 0.31338679011658144 2201 86-87 0.20609027889444675 2201 88-89 0.2730503555745223 2201 90-91 0.1216995737596065 2201 92-93 0.17302467751428097 2201 94-95 0.26646428583791604 2201 96-97 0.31408754837064734 2201 98-99 0.35404937634446654 2201 100-101 0.21733815511333532 1101 1 0.007601534711380253 1101 2 0.009700151390685363 1101 3 -0.08852037450908767 1101 4 -0.011394175424662478 1101 5 -0.08797621517691567 1101 6 0.09015684543609126 1101 7 0.12953494886157557 1101 8 0.07015806570897354 1101 9 0.028392794883508543 1101 10-11 0.030409755928438642 1101 12-13 0.07761402837268605 1101 14-15 0.2734467751803109 1101 16-17 0.1442937267522666 1101 18-19 0.13835036215811414 1101 20-21 0.12309961144362802 1101 22-23 0.2054259288269975 1101 24-25 0.14749441306028643 1101 26-27 0.15780875460922772 1101 28-29 -0.007977746426810484 1101 30-31 0.020106858473305067 1101 32-33 -0.022867755966494485 1101 34-35 0.050951188728262764 1101 36-37 0.030166713261664313 1101 38-39 -0.014468674140346138 1101 40-41 -0.04707676550641082 1101 42-43 0.10046596689908682 1101 44-45 0.017223066473768256 1101 46-47 0.1056134752759803 1101 48-49 0.03926830576909879 1101 50-51 0.03507824321867048 1101 52-53 -0.03218640514823079 1101 54-55 0.17957673329681967 1101 56-57 0.227837295789179 1101 58-59 0.24007689216607275 1101 60-61 0.12339139735257021 1101 62-63 0.053145329807037456 1101 64-65 0.21567773536130375 1101 66-67 0.1398884125818185 1101 68-69 0.12731578262896193 1101 70-71 0.2292292833444165 1101 72-73 0.05697605696970243 1101 74-75 0.07542850083314079 1101 76-77 0.25373030647743633 1101 78-79 0.17733019743327816 1101 80-81 0.18497560102837696 1101 82-83 0.1537512853560017 1101 84-85 0.3232795997293607 1101 86-87 0.23463208174930017 1101 88-89 0.3270257541914283 1101 90-91 0.2270253359023542 1101 92-93 0.14629688884144443 1101 94-95 0.27304669408883697 1101 96-97 0.14010025245347535 1101 98-99 0.03734159767414624 1101 100-101 0.03041740679010374 2304 1 0.11460859617193364 2304 2 0.04168693601435436 2304 3 0.08858960753057943 2304 4 0.13594771288778418 2304 5 0.13213242221964094 2304 6 0.15692346708281946 2304 7 0.25580828062278727 2304 8 0.1189706053098405 2304 9 0.17982846044015588 2304 10-11 0.08895324185316156 2304 12-13 0.15211956832609985 2304 14-15 0.2681255198148378 2304 16-17 0.1832401411167197 2304 18-19 0.2789928550240006 2304 20-21 0.30873658400766857 2304 22-23 0.23231372913856774 2304 24-25 0.23839605342959658 2304 26-27 0.23379270226224236 2304 28-29 0.2626764152971859 2304 30-31 0.1970361057113692 2304 32-33 0.14862299648578414 2304 34-35 0.21107731005818664 2304 36-37 0.26090446259827615 2304 38-39 0.21322769853897938 2304 40-41 0.20669752501273564 2304 42-43 0.26151787024199535 2304 44-45 0.15303574184905955 2304 46-47 0.2241339688755204 2304 48-49 0.23179962618718974 2304 50-51 0.18435782000744183 2304 52-53 0.21399359956814124 2304 54-55 0.2837296289839628 2304 56-57 0.37011325014039187 2304 58-59 0.336111832639709 2304 60-61 0.23662495137887873 2304 62-63 0.26716220692404846 2304 64-65 0.3431676773356003 2304 66-67 0.4683160019428243 2304 68-69 0.3564686038783762 2304 70-71 0.39112382175904514 2304 72-73 0.27673520923713824 2304 74-75 0.424765275571481 2304 76-77 0.35475592437011727 2304 78-79 0.3842543501532987 2304 80-81 0.32705980379436284 2304 82-83 0.2833718583954905 2304 84-85 0.30389943970611455 2304 86-87 0.32654704289876335 2304 88-89 0.20122316661693063 2304 90-91 0.18475095963096066 2304 92-93 0.1979338191248594 2304 94-95 0.0306389700729639 2304 96-97 0.11253171912681381 2304 98-99 0.13665291470978147 2304 100-101 0.17553807332337712 2202 1 0.16366344076923767 2202 2 0.06405416324200175 2202 3 0.09950102904852542 2202 4 0.14062495952758525 2202 5 0.06612939962136721 2202 6 0.1720418653694864 2202 7 0.23984983211773425 2202 8 0.22053557798513168 2202 9 0.2358627112788909 2202 10-11 0.24575989051434988 2202 12-13 0.19267137713752192 2202 14-15 0.33051355707772956 2202 16-17 0.27026828873346176 2202 18-19 0.24868075724584315 2202 20-21 0.3628458406489372 2202 22-23 0.3607121740565873 2202 24-25 0.32957097641400424 2202 26-27 0.391119997432412 2202 28-29 0.23714554839700952 2202 30-31 0.3258386187407254 2202 32-33 0.24473100557995053 2202 34-35 0.3016997384758824 2202 36-37 0.2326438281163874 2202 38-39 0.18820316960010786 2202 40-41 0.2820377651325785 2202 42-43 0.21425792415291056 2202 44-45 0.25638552451738406 2202 46-47 0.36124506650597255 2202 48-49 0.3766661544719554 2202 50-51 0.2010341356025691 2202 52-53 0.19133479447034318 2202 54-55 0.40002548227453616 2202 56-57 0.3415540483595336 2202 58-59 0.29220516393336027 2202 60-61 0.25646075255204437 2202 62-63 0.38638663088961067 2202 64-65 0.3715485827435714 2202 66-67 0.38648851687118224 2202 68-69 0.26545951498339093 2202 70-71 0.4168952305113933 2202 72-73 0.39428256591428124 2202 74-75 0.47741380752767526 2202 76-77 0.3400938830349034 2202 78-79 0.2963202802484517 2202 80-81 0.2746648034778616 2202 82-83 0.31227756440693355 2202 84-85 0.3894615216591504 2202 86-87 0.35101202190410064 2202 88-89 0.31503704735881044 2202 90-91 0.4031136397735935 2202 92-93 0.41956287035604944 2202 94-95 0.4080683240914915 2202 96-97 0.4395876002695971 2202 98-99 0.3664928304146322 2202 100-101 0.3552063523941271 1102 1 0.0749792805902807 1102 2 -0.07098594036511585 1102 3 0.031408783393693795 1102 4 0.11350403059559255 1102 5 0.05641446636260383 1102 6 0.09653582407380412 1102 7 0.1594253961436891 1102 8 0.21348146387013145 1102 9 0.10093975435531632 1102 10-11 0.0542957041392782 1102 12-13 0.10523786082382003 1102 14-15 0.18691880218803192 1102 16-17 0.24231526514223134 1102 18-19 0.21952477261031333 1102 20-21 0.20632492054710383 1102 22-23 0.29753773440332054 1102 24-25 0.19626540900314637 1102 26-27 0.3558138119109131 1102 28-29 0.22166275983500583 1102 30-31 0.27956597308000397 1102 32-33 0.2131167066204398 1102 34-35 0.17091164740757847 1102 36-37 0.27057933857276595 1102 38-39 0.34115141019231743 1102 40-41 0.21115636879120103 1102 42-43 0.24231131713882093 1102 44-45 0.266442773336351 1102 46-47 0.4088875630842921 1102 48-49 0.41523861322778544 1102 50-51 0.35900732137424995 1102 52-53 0.4233523370390344 1102 54-55 0.5338902926470226 1102 56-57 0.5919203524994003 1102 58-59 0.4318328258562758 1102 60-61 0.4594497590127702 1102 62-63 0.5824386699188793 1102 64-65 0.460322810473194 1102 66-67 0.5707914499533473 1102 68-69 0.37587644532820974 1102 70-71 0.3815958147731209 1102 72-73 0.560592592551906 1102 74-75 0.6616984916289823 1102 76-77 0.5938203543589822 1102 78-79 0.5113165274338485 1102 80-81 0.44589940590027055 1102 82-83 0.45717064598282064 1102 84-85 0.5265661148098957 1102 86-87 0.5219549349712516 1102 88-89 0.42685708886052254 1102 90-91 0.2664835201250355 1102 92-93 0.33513925852953363 1102 94-95 0.37822800267395706 1102 96-97 0.46178218738640453 1102 98-99 0.5314150514922069 1102 100-101 0.34047618712311234 2305 1 0.013991597845947723 2305 2 -0.07076875760159851 2305 3 0.041680837420784655 2305 4 -0.020935937172339436 2305 5 -0.12620400568375345 2305 6 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0.3172266035144631 1213 70-71 0.3664672261208253 1213 72-73 0.40657085440681584 1213 74-75 0.37259457821895836 1213 76-77 0.39133179906631455 1213 78-79 0.3813303970593722 1213 80-81 0.4359428277997708 1213 82-83 0.3092054922838017 1213 84-85 0.33271461951922277 1213 86-87 0.38123172011896855 1213 88-89 0.28992563019291495 1213 90-91 0.38721144883697534 1213 92-93 0.4363639555280763 1213 94-95 0.5373573890563428 1213 96-97 0.5135204231337767 1213 98-99 0.39589883853453145 1213 100-101 0.5037615659995858 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1615.0 3 385.0 4 375.0 5 386.0 6 1107.0 7 4221.0 8 17573.0 9 42478.0 10 49661.0 11 31768.0 12 18318.0 13 12501.0 14 9843.0 15 8586.0 16 8198.0 17 8658.0 18 9262.0 19 10205.0 20 10990.0 21 13056.0 22 14658.0 23 16449.0 24 18365.0 25 21742.0 26 25670.0 27 30584.0 28 36899.0 29 45106.0 30 55879.0 31 70669.0 32 93493.0 33 130696.0 34 192932.0 35 310872.0 36 546235.0 37 938546.0 38 462139.0 39 1444.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.25566418842296 25.640180596734435 20.943065184114673 16.16109003072793 2 31.238138440036288 26.089547115219574 29.16601279945075 13.50630164529338 3 36.09217231490665 22.312164268535533 25.59723469573165 15.99842872082616 4 29.675220567352202 21.712461117124583 30.88126756105833 17.731050754464885 5 32.61693562347742 19.813510885987213 30.250571192999043 17.318982297536326 6 29.55227674319611 20.252985076912243 33.3836179836529 16.811120196238758 7 33.907046102236 20.692722886048404 26.972714840742434 18.42751617097316 8 28.99343417583723 29.321327418132302 24.17143511381211 17.51380329221836 9 30.656078891068745 26.990166162171096 24.257656957294884 18.096097989465278 10-11 26.87399558812346 29.90730224169659 27.793674616990394 15.425027553189551 12-13 27.974836333784957 27.117896759242893 27.53205718297146 17.37520972400069 14-15 26.06621681392227 27.25726546872585 28.368041579494307 18.30847613785757 16-17 26.253912942341344 27.64657225868308 28.750819699727824 17.348695099247752 18-19 25.76309710289273 27.818583027440603 29.1524431455724 17.265876724094266 20-21 24.7052207372676 28.870088701958206 28.818671712468852 17.606018848305343 22-23 26.090441184911928 28.18115789513966 28.027078015758516 17.701322904189897 24-25 23.757916144354326 30.106293507670383 28.24614943860773 17.889640909367564 26-27 22.661142882350564 31.054866758896527 28.255130949653033 18.028859409099876 28-29 23.35236932040393 30.367013667383414 28.11508202053216 18.165534991680495 30-31 24.675707682738143 28.60107561460955 28.47044818910412 18.25276851354819 32-33 24.679816551743173 28.48592321362583 28.559446649891978 18.274813584739018 34-35 22.8504429550126 28.502436053219622 28.370113862409458 20.27700712935832 36-37 21.207607218000657 28.438027748755484 28.67280934146155 21.681555691782307 38-39 20.829775126435507 29.370299735835243 29.650442878876003 20.149482258853247 40-41 21.395857671123096 28.644718516880523 31.006464280533276 18.952959531463108 42-43 22.340770119120467 28.40628254264764 30.45984151011688 18.793105828115007 44-45 21.564601911035567 29.216503018050034 30.362863685954128 18.856031384960275 46-47 22.647975144770545 29.8755980919949 28.467185189434012 19.00924157380054 48-49 20.872786586972396 29.7284477192694 29.707053936563373 19.69171175719483 50-51 20.454266948356448 28.536258612690613 30.632536703386027 20.376937735566912 52-53 23.052189670266344 28.438272332854847 28.61337725549754 19.89616074138127 54-55 23.79486583451426 28.392439910808832 28.699245383351208 19.1134488713257 56-57 24.14535252200574 28.322122868413018 28.41828705077441 19.114237558806828 58-59 23.225759758909746 28.643267782499276 28.291366048796736 19.839606409794243 60-61 21.35314380811012 28.45439941820706 28.245194942485245 21.947261831197572 62-63 22.41714536123922 28.550372790886563 28.297650872517515 20.7348309753567 64-65 21.156188897992845 29.247472374515493 29.66941394959542 19.92692477789624 66-67 20.38034949849877 29.221870080831703 29.000537692356872 21.397242728312655 68-69 20.143916103105123 28.743179199922736 31.29436346480931 19.818541232162826 70-71 20.000162463759853 30.30119248399732 30.077513605573547 19.621131446669278 72-73 19.888011549814706 32.26050533504578 28.249090390787124 19.60239272435239 74-75 19.77959650928194 32.439525815268404 28.26790471863001 19.512972956819645 76-77 19.879386791672108 32.41327870168928 28.06377349445745 19.643561012181166 78-79 19.80099731245215 32.4308169087912 28.177165115981445 19.5910206627752 80-81 19.76057000164472 32.10670715771567 28.227852893762652 19.904869946876957 82-83 19.844599051201453 31.998953138698084 28.062000314610337 20.094447495490126 84-85 19.848352334005057 31.649260349700253 28.302954998570446 20.19943231772424 86-87 20.094992379851778 31.273085231752617 28.327098062668725 20.30482432572688 88-89 20.151780482352784 31.330516189785246 28.25653136875338 20.261171959108594 90-91 20.221039077750312 31.065509168517956 28.24727702428928 20.466174729442447 92-93 20.269975523236983 31.088370632973167 28.127723057129156 20.51393078666069 94-95 20.160951645841394 30.96861586979672 28.320267596748472 20.550164887613413 96-97 20.28944005972451 30.85534358839143 28.202140485966808 20.65307586591725 98-99 20.31543803914276 30.86516262570064 28.166311728861142 20.653087606295454 100-101 20.470780579687837 30.784835650246972 28.03963257675527 20.704751193309924 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 899.0 1 721.0 2 403.0 3 283.5 4 321.0 5 387.5 6 519.5 7 669.0 8 830.0 9 993.5 10 1100.5 11 1215.0 12 1238.0 13 1141.5 14 1082.0 15 1061.5 16 1061.5 17 1159.0 18 1390.5 19 1901.0 20 2762.5 21 3997.5 22 5831.5 23 8305.0 24 11560.0 25 15632.5 26 20819.5 27 27141.5 28 34016.5 29 41904.5 30 50365.5 31 59338.0 32 69545.0 33 80925.5 34 92927.0 35 105334.0 36 118779.5 37 132020.5 38 143146.5 39 150480.0 40 153387.0 41 153118.0 42 150580.5 43 147926.5 44 144953.0 45 139918.5 46 134262.0 47 128144.0 48 120773.0 49 112264.0 50 103315.5 51 94222.0 52 84476.5 53 74338.5 54 64075.0 55 54130.5 56 44982.0 57 36959.5 58 30227.5 59 24421.5 60 19464.5 61 15139.5 62 11585.0 63 8813.5 64 6616.5 65 4973.5 66 3691.5 67 2700.5 68 2042.5 69 1590.5 70 1200.5 71 908.5 72 687.0 73 531.0 74 420.5 75 338.0 76 284.0 77 227.0 78 182.5 79 152.0 80 136.0 81 103.0 82 82.0 83 82.5 84 68.0 85 52.0 86 45.5 87 40.0 88 34.5 89 26.5 90 22.0 91 24.5 92 19.0 93 11.0 94 11.0 95 7.0 96 5.5 97 5.5 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2968305067545675 2 0.27277473404157765 3 0.24395059977429756 4 0.2321519615694512 5 0.2378373157303357 6 0.18810575003270605 7 0.15475778557289419 8 0.15136491292849535 9 0.1306408800194647 10-11 0.11595371510384636 12-13 0.13670831443309683 14-15 0.1396579739843084 16-17 0.133315441788698 18-19 0.12050811171659793 20-21 0.12720215774473614 22-23 0.1551704322458616 24-25 0.5624832648849297 26-27 0.22178994511493585 28-29 0.19394393629468965 30-31 0.29389613041346585 32-33 0.3270301299317391 34-35 0.3350690984495489 36-37 0.3258686059633863 38-39 0.3334643613880089 40-41 0.33717818144471573 42-43 0.3490379524900017 44-45 0.3290933632965762 46-47 0.34861002260692436 48-49 0.34451412229746997 50-51 0.3507955216526408 52-53 0.3346258853563617 54-55 0.32715239561261833 56-57 0.3360625071066927 58-59 0.32738164376426687 60-61 0.3032953046310572 62-63 0.28978494689390155 64-65 0.3012779208965498 66-67 0.31805888559722506 68-69 0.3038760666152336 70-71 0.28420657520378634 72-73 0.28041633909652997 74-75 0.27239265378883004 76-77 0.27466985209520584 78-79 0.2841607255734566 80-81 0.2936974486820371 82-83 0.3174934068231586 84-85 0.30739120494051164 86-87 0.31008104991985486 88-89 0.31608735149304734 90-91 0.30885839311106245 92-93 0.2900906110960996 94-95 0.28555149769345795 96-97 0.3032647382108374 98-99 0.3116093709308453 100-101 0.3135197721945834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3271564.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.95659347138937 #Duplication Level Percentage of deduplicated Percentage of total 1 97.78748504285842 91.87778978761418 2 1.5932895268799054 2.994001127185552 3 0.1949745696712341 0.5495743913957768 4 0.14641046122657353 0.5502491274169513 5 0.06663188267399821 0.3130252356317082 6 0.03335681118667544 0.18804554089010145 7 0.019084408213170395 0.12551741888888265 8 0.023407055627116955 0.17593977679353798 9 0.019343780880592455 0.16357281807577137 >10 0.1026788130007066 1.7655825387686352 >50 0.009847076809401804 0.6225519596416731 >100 0.003419544030678578 0.5465949230845685 >500 3.8463181450087656E-5 0.02753768561379094 >1k 3.256376003231883E-5 0.10001766899885359 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001895118053628173 0.0 0.0 2 0.0022313486760460748 0.0 0.0 3 0.002689844979343213 0.0 0.0 4 0.002934376341101687 0.0 0.0 5 0.003515138325278063 0.0 0.0 6 0.004218165990333676 0.0 0.0 7 0.005074025756488334 0.0 0.0 8 0.005991018363082612 0.0 0.0 9 0.006663479607918414 0.0 6.113284043961848E-5 10-11 0.009613139159130007 0.0 6.113284043961848E-5 12-13 0.012196001667703887 0.0 7.64160505495231E-5 14-15 0.015283210109904619 0.0 1.0698247076933233E-4 16-17 0.018294002501555832 0.0 1.2226568087923695E-4 18-19 0.021885556877383416 0.0 1.2226568087923695E-4 20-21 0.02590504113628833 0.0 1.2226568087923695E-4 22-23 0.029863392554753627 0.0 1.2226568087923695E-4 24-25 0.03476930300003302 0.0 1.2226568087923695E-4 26-27 0.04014899295871944 0.0 1.2226568087923695E-4 28-29 0.04707228713850623 0.0 1.2226568087923695E-4 30-31 0.07808192045150271 0.0 1.2226568087923695E-4 32-33 0.08644183638162053 0.0 1.2226568087923695E-4 34-35 0.09539779750602465 0.0 1.2226568087923695E-4 36-37 0.1051790519763636 0.0 1.2226568087923695E-4 38-39 0.11580088300274731 0.0 1.2226568087923695E-4 40-41 0.12685064391220835 0.0 1.2226568087923695E-4 42-43 0.13851173322606558 1.528321010990462E-5 1.2226568087923695E-4 44-45 0.1524653040564085 3.056642021980924E-5 1.2226568087923695E-4 46-47 0.16719831860235657 3.056642021980924E-5 1.2226568087923695E-4 48-49 0.18404041614347144 3.056642021980924E-5 1.2226568087923695E-4 50-51 0.20163139097997168 3.056642021980924E-5 1.528321010990462E-4 52-53 0.22119389992064958 3.056642021980924E-5 2.139649415386647E-4 54-55 0.2417803839386911 3.056642021980924E-5 2.445313617584739E-4 56-57 0.26369650723629434 3.056642021980924E-5 2.445313617584739E-4 58-59 0.2888679542873072 3.056642021980924E-5 2.7509778197828315E-4 60-61 0.3160415018627176 3.056642021980924E-5 3.056642021980924E-4 62-63 0.34614942577922975 3.056642021980924E-5 3.20947412307997E-4 64-65 0.37812190132915025 3.056642021980924E-5 3.3623062241790164E-4 66-67 0.41298290358984263 3.056642021980924E-5 3.3623062241790164E-4 68-69 0.449463926122185 3.056642021980924E-5 3.3623062241790164E-4 70-71 0.4900714153842015 3.056642021980924E-5 3.3623062241790164E-4 72-73 0.5359516121341352 3.056642021980924E-5 3.3623062241790164E-4 74-75 0.5861263909249521 3.056642021980924E-5 3.3623062241790164E-4 76-77 0.6411459473206087 3.056642021980924E-5 3.3623062241790164E-4 78-79 0.6997112084617633 3.056642021980924E-5 3.667970426377109E-4 80-81 0.7644050368569895 3.056642021980924E-5 3.667970426377109E-4 82-83 0.8374893476025533 3.056642021980924E-5 3.820802527476155E-4 84-85 0.9183680955041686 3.056642021980924E-5 3.9736346285752013E-4 86-87 1.0104035867860142 3.056642021980924E-5 3.9736346285752013E-4 88-89 1.110737861157538 3.056642021980924E-5 3.9736346285752013E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 2320 0.0 80.62108 2 GAGCGGC 1575 0.0 77.36553 9 GATCGGG 2590 0.0 73.8843 1 AGAGCGG 1970 0.0 66.43104 8 AAGCGGC 770 0.0 64.653725 9 TCGGGAG 2330 0.0 64.36333 3 CGGAAAG 1345 0.0 62.450848 5 ATCCGGA 520 0.0 62.079178 2 GAGAGCG 2175 0.0 61.915665 7 CGGGAGA 2580 0.0 61.250813 4 GTTCGGA 450 0.0 61.18654 2 GATCCGG 565 0.0 60.51053 1 GGTTCGG 560 0.0 57.659096 1 TCGGGAA 1005 0.0 55.721554 3 TCGGAAA 1065 0.0 51.68871 4 TCCGGAG 480 0.0 51.41258 3 GGATCGG 925 0.0 51.33401 1 TCGGAGA 720 0.0 50.751114 4 AAAGCGG 1105 0.0 50.20556 8 CGGGAAA 1485 0.0 47.934933 4 >>END_MODULE