FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_50331580-1.342000000357a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_50331580-1.342000000357a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141398
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA13600.077.849492
GAGCGGC9650.073.7089849
AGAGCGG12500.061.459518
GATCGGG17900.060.4908181
GTTCGGA3700.060.309532
AAGCGGC4050.059.713379
CGGGAGA16650.057.011184
TTGTGTT302650.056.615261
GAGAGCG16450.053.6205337
TCGGGAG17750.052.9453
TCGGAAA6200.052.8203964
GATCCGG3650.052.0444871
TCGGAGA5600.051.6994674
TTCGGAA4300.050.7747153
TTCGGAG4650.048.9943963
GGTTCGG5550.046.2070661
GGATCGG6000.044.3245541
AAAGCGG5600.044.0351458
TCGGGAA6500.043.8122943
CGGAAAG10850.042.868735
Produced by FastQC (version 0.11.2)