##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_50331580-1.342000000357a5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3141398 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.688793970073196 33.0 33.0 33.0 27.0 33.0 2 31.534805204561792 33.0 33.0 33.0 27.0 33.0 3 31.46884253443849 33.0 33.0 33.0 27.0 33.0 4 35.11751965207847 37.0 37.0 37.0 33.0 37.0 5 35.14905783985347 37.0 37.0 37.0 33.0 37.0 6 35.29429795269495 37.0 37.0 37.0 33.0 37.0 7 35.16446722128173 37.0 37.0 37.0 33.0 37.0 8 35.13737959978328 37.0 37.0 37.0 33.0 37.0 9 34.97350383491681 37.0 37.0 37.0 33.0 37.0 10-11 34.94304080539938 37.0 37.0 37.0 33.0 37.0 12-13 34.9097317500043 37.0 37.0 37.0 33.0 37.0 14-15 36.585613952768796 40.0 37.0 40.0 33.0 40.0 16-17 36.54748968452899 40.0 37.0 40.0 33.0 40.0 18-19 36.53710370351035 40.0 37.0 40.0 33.0 40.0 20-21 36.52298101036545 40.0 37.0 40.0 33.0 40.0 22-23 36.6948385718715 40.0 37.0 40.0 33.0 40.0 24-25 36.34639720914065 40.0 37.0 40.0 33.0 40.0 26-27 36.22995478446221 40.0 37.0 40.0 33.0 40.0 28-29 36.14615403715162 40.0 37.0 40.0 33.0 40.0 30-31 36.13928862245408 40.0 37.0 40.0 33.0 40.0 32-33 35.99358470337092 40.0 37.0 40.0 27.0 40.0 34-35 35.98820413713894 40.0 37.0 40.0 27.0 40.0 36-37 35.86121624830729 40.0 37.0 40.0 27.0 40.0 38-39 35.76903833898156 40.0 37.0 40.0 27.0 40.0 40-41 35.841575788868525 38.5 37.0 40.0 27.0 40.0 42-43 35.712204725412064 37.0 37.0 40.0 27.0 40.0 44-45 35.512024423521 37.0 37.0 40.0 27.0 40.0 46-47 35.375676211673905 37.0 37.0 40.0 27.0 40.0 48-49 35.25169637849136 37.0 37.0 40.0 27.0 40.0 50-51 34.17698219073164 37.0 35.0 38.5 24.5 38.5 52-53 34.60782046719327 37.0 35.0 38.5 27.0 40.0 54-55 35.347516456049185 37.0 37.0 40.0 27.0 40.0 56-57 35.48809638256598 37.0 37.0 40.0 27.0 40.0 58-59 35.250479404392564 37.0 37.0 40.0 27.0 40.0 60-61 35.021457803181896 37.0 37.0 40.0 27.0 40.0 62-63 34.774978687832615 37.0 33.0 40.0 27.0 40.0 64-65 34.573735165044354 37.0 33.0 40.0 27.0 40.0 66-67 34.327242839016264 37.0 33.0 40.0 27.0 40.0 68-69 34.17083922508387 37.0 33.0 38.5 27.0 40.0 70-71 33.87058421123334 37.0 33.0 37.0 22.0 40.0 72-73 33.729290112236654 37.0 33.0 37.0 22.0 40.0 74-75 33.568339955650316 37.0 33.0 37.0 27.0 40.0 76-77 33.31427424987219 37.0 33.0 37.0 22.0 38.5 78-79 33.01483129485662 37.0 33.0 37.0 22.0 37.0 80-81 32.67500982046847 37.0 33.0 37.0 22.0 37.0 82-83 32.39382466023089 37.0 33.0 37.0 22.0 37.0 84-85 32.098755394891064 37.0 33.0 37.0 22.0 37.0 86-87 31.938849518590132 35.0 33.0 37.0 22.0 37.0 88-89 31.79303147834181 33.0 33.0 37.0 18.5 37.0 90-91 31.629323791509385 33.0 33.0 37.0 15.0 37.0 92-93 31.448282261591814 33.0 33.0 37.0 15.0 37.0 94-95 31.291280824651956 33.0 33.0 37.0 15.0 37.0 96-97 31.149582447050644 33.0 33.0 37.0 15.0 37.0 98-99 30.992267455444996 33.0 33.0 37.0 15.0 37.0 100-101 29.67647350001496 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14774750511375245 2306 2 0.11223356999090228 2306 3 0.12319730832718534 2306 4 0.28649457212094376 2306 5 0.20067758636120203 2306 6 0.1883983924929069 2306 7 0.24807474835939303 2306 8 0.25143453497487656 2306 9 0.271986237246864 2306 10-11 0.2680489885545896 2306 12-13 0.27459477392356035 2306 14-15 0.45558497449797386 2306 16-17 0.3522385045344194 2306 18-19 0.2940607062750118 2306 20-21 0.3350678379087384 2306 22-23 0.3679988211193219 2306 24-25 0.538611396561059 2306 26-27 0.3359251981070628 2306 28-29 0.25521524684151586 2306 30-31 0.21834742281055242 2306 32-33 0.20822944310171465 2306 34-35 0.3385143100334176 2306 36-37 0.2643215491155928 2306 38-39 0.2917784416904112 2306 40-41 0.10121060381945313 2306 42-43 0.25360967623297626 2306 44-45 0.41296191816017114 2306 46-47 0.292619880998366 2306 48-49 0.3443564270380932 2306 50-51 0.29578897104624247 2306 52-53 0.23361573124157076 2306 54-55 0.31222459518996004 2306 56-57 0.2278938483802122 2306 58-59 0.31324507262621637 2306 60-61 0.21977779800437958 2306 62-63 0.2584247322796642 2306 64-65 0.20621547419113284 2306 66-67 0.1965613365004799 2306 68-69 0.053389560146946735 2306 70-71 0.15788109927444083 2306 72-73 0.003761348519226715 2306 74-75 0.07860170889296825 2306 76-77 0.1406027693067884 2306 78-79 0.16520515849921935 2306 80-81 0.2545755611242768 2306 82-83 0.3092834025743656 2306 84-85 0.34328072166578494 2306 86-87 0.2878865215269677 2306 88-89 0.23999333561012648 2306 90-91 0.13789500264148558 2306 92-93 0.15685417737563156 2306 94-95 -0.018359148814329984 2306 96-97 0.019892313040607235 2306 98-99 0.23342613680085478 2306 100-101 0.1645222895366345 2307 1 0.14323661712121805 2307 2 0.1502778731468588 2307 3 0.09539417183483678 2307 4 0.2158659547882067 2307 5 0.1915230665807499 2307 6 0.144377188472383 2307 7 0.2921597517234389 2307 8 0.2126877530257687 2307 9 0.345363199955635 2307 10-11 0.3596296876067484 2307 12-13 0.2787112893611976 2307 14-15 0.5078925470169935 2307 16-17 0.4928718090608655 2307 18-19 0.3961629803957507 2307 20-21 0.393067786800664 2307 22-23 0.36040404093464673 2307 24-25 0.3280218546111868 2307 26-27 0.24610969997740284 2307 28-29 0.23367284969094726 2307 30-31 0.24113004832847196 2307 32-33 0.2769744362681621 2307 34-35 0.24671297158493388 2307 36-37 0.4055160992110274 2307 38-39 0.30826662740687283 2307 40-41 0.048223019480403195 2307 42-43 0.17646160636034125 2307 44-45 0.37727398887590624 2307 46-47 0.3805450663208845 2307 48-49 0.3391301117297303 2307 50-51 0.15065403972229063 2307 52-53 0.21306256692275838 2307 54-55 0.2785989110834919 2307 56-57 0.09779180887641559 2307 58-59 0.26907963406832636 2307 60-61 0.2681847781327704 2307 62-63 0.4377740338569325 2307 64-65 0.4406175208466294 2307 66-67 0.4343659290584938 2307 68-69 0.39603919833435697 2307 70-71 0.3684688404363854 2307 72-73 0.24587797063210814 2307 74-75 0.3232871978523306 2307 76-77 0.2438548036397492 2307 78-79 0.1618117880399268 2307 80-81 0.33214947446452925 2307 82-83 0.28264623338554173 2307 84-85 0.3930950604310439 2307 86-87 0.37512697868013944 2307 88-89 0.2257336678229116 2307 90-91 0.3752318190988362 2307 92-93 0.4109579586701102 2307 94-95 0.3012428907752245 2307 96-97 0.17153220484926734 2307 98-99 0.2126012250094469 2307 100-101 0.26002776298864916 2201 1 0.17829034078243566 2201 2 0.17390083954061453 2201 3 0.20091092646954323 2201 4 0.2166094478767775 2201 5 0.2067125944626369 2201 6 0.1828017733105085 2201 7 0.1850141366265774 2201 8 0.07401372086879121 2201 9 0.19422578124152068 2201 10-11 0.21499424166353975 2201 12-13 0.29746293932321777 2201 14-15 0.4829612842101412 2201 16-17 0.3975709008858459 2201 18-19 0.46460654765403575 2201 20-21 0.38412072745949644 2201 22-23 0.4177553737447752 2201 24-25 0.5144922558639919 2201 26-27 0.34259588698925114 2201 28-29 0.3487526494865776 2201 30-31 0.41404696566964105 2201 32-33 0.3658173706593857 2201 34-35 0.40650831343909744 2201 36-37 0.4006115590543189 2201 38-39 0.33509074459862376 2201 40-41 0.3652357217186122 2201 42-43 0.3701110260226841 2201 44-45 0.4016162040183886 2201 46-47 0.3999890268762414 2201 48-49 0.4081465637591606 2201 50-51 0.29818174561510347 2201 52-53 0.338225318278198 2201 54-55 0.4288168407605397 2201 56-57 0.42695990476188683 2201 58-59 0.4361581551995144 2201 60-61 0.4691353358423811 2201 62-63 0.5174579954179777 2201 64-65 0.45354693749189323 2201 66-67 0.35735513044993894 2201 68-69 0.3849155360382497 2201 70-71 0.38075174135246215 2201 72-73 0.389769025548226 2201 74-75 0.4549249838233038 2201 76-77 0.2726310391582487 2201 78-79 0.5295957296667382 2201 80-81 0.6099949967145406 2201 82-83 0.48462582863077586 2201 84-85 0.4622937939785601 2201 86-87 0.3603424210675108 2201 88-89 0.48310888579748834 2201 90-91 0.47889612658171643 2201 92-93 0.4533783698900322 2201 94-95 0.60273382788181 2201 96-97 0.4607303637161415 2201 98-99 0.2737059408029303 2201 100-101 0.2790649573977255 1101 1 0.07980746447745446 1101 2 0.0735924418348155 1101 3 0.06785916919581325 1101 4 0.17360373511201743 1101 5 0.19310178720382964 1101 6 0.16021273246303025 1101 7 0.3451491996999607 1101 8 0.20786351944519765 1101 9 0.27110545050541646 1101 10-11 0.33144149947806056 1101 12-13 0.35539969289796147 1101 14-15 0.43978691164763717 1101 16-17 0.424556084333922 1101 18-19 0.4774766348899533 1101 20-21 0.4380675818735895 1101 22-23 0.45415238861234997 1101 24-25 0.43513340283053026 1101 26-27 0.3286131829554648 1101 28-29 0.36957602587285265 1101 30-31 0.4047459349008662 1101 32-33 0.2529925718447785 1101 34-35 0.20154946950328423 1101 36-37 0.2809719055145763 1101 38-39 0.22241939162279323 1101 40-41 0.20466575456539715 1101 42-43 0.2291548486789381 1101 44-45 0.3693745977439633 1101 46-47 0.37026625287052894 1101 48-49 0.3247543211570658 1101 50-51 0.246653065458311 1101 52-53 0.2097817001344282 1101 54-55 0.4140503873249344 1101 56-57 0.4294801201177805 1101 58-59 0.47072396264119476 1101 60-61 0.4173759106411197 1101 62-63 0.4517369198433343 1101 64-65 0.507500476827353 1101 66-67 0.4747510323933639 1101 68-69 0.44856052091803633 1101 70-71 0.38472565817237125 1101 72-73 0.290401818668208 1101 74-75 0.37291943979518294 1101 76-77 0.42470669955933005 1101 78-79 0.4303009811872869 1101 80-81 0.4005526451712811 1101 82-83 0.4918399345217779 1101 84-85 0.4517250869714431 1101 86-87 0.4934657146836976 1101 88-89 0.495167715564353 1101 90-91 0.3189239604428238 1101 92-93 0.3709047276897657 1101 94-95 0.4001527413223762 1101 96-97 0.33310038502490613 1101 98-99 0.4064851530640894 1101 100-101 0.43177729893051264 2304 1 0.1621620582719956 2304 2 0.1098611943473351 2304 3 0.20167210774421562 2304 4 0.16928077847349243 2304 5 0.07997628470045015 2304 6 0.15215040833921023 2304 7 0.2370789954094974 2304 8 0.05796619002224901 2304 9 0.2154534866308424 2304 10-11 0.18598033271089065 2304 12-13 0.2764104441861974 2304 14-15 0.3676138643300959 2304 16-17 0.29277714242331143 2304 18-19 0.373155362779805 2304 20-21 0.32716808369173833 2304 22-23 0.0876131465244967 2304 24-25 0.20105519388962279 2304 26-27 0.20962694926398484 2304 28-29 0.24275573592839805 2304 30-31 0.22047666047006942 2304 32-33 0.2375575809355226 2304 34-35 0.19554369756671974 2304 36-37 0.2207136130667493 2304 38-39 0.21446384946732167 2304 40-41 0.24197106305678062 2304 42-43 0.21400651310718644 2304 44-45 0.22084973067894964 2304 46-47 0.022849405759465924 2304 48-49 0.039617200547120035 2304 50-51 0.25949231234279324 2304 52-53 0.2755826619715833 2304 54-55 0.42242948843774997 2304 56-57 0.38776063349350665 2304 58-59 0.27547456107703994 2304 60-61 0.41470536213584097 2304 62-63 0.32254515655896654 2304 64-65 0.3266549349459851 2304 66-67 0.3768244013130655 2304 68-69 0.2562824561042376 2304 70-71 0.39314116222895024 2304 72-73 0.2944571066026427 2304 74-75 0.37987197378268434 2304 76-77 0.38889548208499747 2304 78-79 0.4512486625910128 2304 80-81 0.37195063812709606 2304 82-83 0.3051884800028972 2304 84-85 0.27796612756457506 2304 86-87 0.2689733287519651 2304 88-89 0.11742393290163378 2304 90-91 0.2986642232977772 2304 92-93 0.3779584761365733 2304 94-95 0.3422320888902597 2304 96-97 0.3000286675140309 2304 98-99 0.2795058322202486 2304 100-101 0.2724434699461149 2202 1 0.1676826820144477 2202 2 0.1563421634955553 2202 3 0.1328938129756061 2202 4 0.16798236298053126 2202 5 0.1417490043516878 2202 6 0.2093833895598678 2202 7 0.3412875802518869 2202 8 0.2862462872724194 2202 9 0.37340431661072415 2202 10-11 0.30579348244261695 2202 12-13 0.27409341702321655 2202 14-15 0.3896182866976332 2202 16-17 0.24770148011614168 2202 18-19 0.3901945772331956 2202 20-21 0.40982601041876165 2202 22-23 0.2939013968598445 2202 24-25 0.3322721833262463 2202 26-27 0.4318320548977894 2202 28-29 0.40726518847702664 2202 30-31 0.35883860295832903 2202 32-33 0.18784105259884143 2202 34-35 0.2116895309000526 2202 36-37 0.29107589090041586 2202 38-39 0.3231409118415698 2202 40-41 0.31508631708093304 2202 42-43 0.2916583616987296 2202 44-45 0.211031445970697 2202 46-47 0.18583041682587265 2202 48-49 0.22630032009775647 2202 50-51 0.17445186130321844 2202 52-53 0.14240914168318142 2202 54-55 0.3679510553391552 2202 56-57 0.3894929021051752 2202 58-59 0.17761029213002644 2202 60-61 0.23630946592523827 2202 62-63 0.3833343710123529 2202 64-65 0.35682030214920246 2202 66-67 0.27885586090395265 2202 68-69 0.3369864718654654 2202 70-71 0.3353538131343825 2202 72-73 0.26193541394670916 2202 74-75 0.14576420215291108 2202 76-77 0.2236891398650087 2202 78-79 0.1894083498814183 2202 80-81 0.20442831669852524 2202 82-83 0.3060478026051783 2202 84-85 0.48230308682011724 2202 86-87 0.4305495569545137 2202 88-89 0.4093861227071365 2202 90-91 0.33455770921327144 2202 92-93 0.3843054119179321 2202 94-95 0.26288622202238443 2202 96-97 0.35895985882858383 2202 98-99 0.31993853258913063 2202 100-101 0.45453707323250114 1102 1 0.06751768524264534 1102 2 0.026411390401985813 1102 3 0.12173979207601349 1102 4 0.09243177592680496 1102 5 0.17837828769179964 1102 6 0.1364865005859599 1102 7 0.18795416720996627 1102 8 0.29183286275246445 1102 9 0.17996772080498857 1102 10-11 0.20381770588172543 1102 12-13 0.15707872274549572 1102 14-15 0.1870046421819822 1102 16-17 0.1966266098418643 1102 18-19 0.1820756599946165 1102 20-21 0.17457977067127928 1102 22-23 0.24616950465773613 1102 24-25 0.19507975883019668 1102 26-27 0.2615873694677262 1102 28-29 0.2703474596934541 1102 30-31 0.27325370135857696 1102 32-33 0.12086760751407155 1102 34-35 0.10288223039071909 1102 36-37 0.23433732708971178 1102 38-39 0.1792194885650673 1102 40-41 0.07560157330418349 1102 42-43 0.11198375207153077 1102 44-45 0.047462254933130055 1102 46-47 0.11935794020040902 1102 48-49 0.2631490013192064 1102 50-51 0.1602645301857919 1102 52-53 0.11972597135390828 1102 54-55 0.23302345066185381 1102 56-57 0.2990952957539932 1102 58-59 0.22688155638790164 1102 60-61 0.28527524702952434 1102 62-63 0.3103597661676716 1102 64-65 0.2333949452540125 1102 66-67 0.22520082371989503 1102 68-69 0.2265247200301772 1102 70-71 0.23764493678630316 1102 72-73 0.223232611392838 1102 74-75 0.24231929672713903 1102 76-77 0.2995138248168274 1102 78-79 0.3169699845450893 1102 80-81 0.2684351078700047 1102 82-83 0.29320943966011015 1102 84-85 0.25131627460556416 1102 86-87 0.2741454702953412 1102 88-89 0.24349591729810882 1102 90-91 0.1791528693079627 1102 92-93 0.25121489086290794 1102 94-95 0.2292630483453486 1102 96-97 0.2576479551484425 1102 98-99 0.33941562434168304 1102 100-101 0.31835196598459703 2305 1 0.01810265806804523 2305 2 -0.03575438072760306 2305 3 0.06103769247583912 2305 4 0.03910455361950227 2305 5 0.03715805828593943 2305 6 0.1347476125356195 2305 7 0.05790885233604115 2305 8 0.17324142487284178 2305 9 0.015379757836925023 2305 10-11 0.10492633928516426 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0.44851765643439023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1435.0 3 333.0 4 368.0 5 354.0 6 1107.0 7 4243.0 8 13907.0 9 27640.0 10 33739.0 11 23633.0 12 14335.0 13 10058.0 14 8184.0 15 7523.0 16 7518.0 17 7879.0 18 8659.0 19 9562.0 20 10479.0 21 12084.0 22 13618.0 23 15176.0 24 17437.0 25 20123.0 26 23464.0 27 28118.0 28 34042.0 29 41946.0 30 51768.0 31 65093.0 32 86970.0 33 122410.0 34 183208.0 35 297942.0 36 545435.0 37 950062.0 38 438729.0 39 2817.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.57394011972989 24.22827026352261 24.318461971185265 15.879327645562233 2 30.257214764832902 23.571067209067394 32.99058246368878 13.181135562410928 3 37.99949647098917 20.692872511751403 25.63578514946802 15.671845867791406 4 29.009266609321998 20.39598451183436 34.46177475796551 16.13297412087814 5 32.010362204945494 18.579507492146025 33.24041936362865 16.169710939279827 6 26.322347324562173 19.37410206161073 38.60038811667737 15.70316249714972 7 33.54872481843579 19.350261367668452 29.882436354095564 17.218577459800198 8 28.800380765720373 29.12236115552067 25.868797135458426 16.20846094330053 9 32.18108383591194 26.778254946794984 23.653077655167227 17.387583562125855 10-11 27.670418141435345 28.953767645239054 27.388655197397387 15.987159015928217 12-13 28.869873651252338 26.216006490914108 26.88753751822621 18.02658233960734 14-15 27.047143950019127 26.645958179268135 27.960187428279994 18.34671044243274 16-17 26.075783422388305 27.49637596624963 28.475359120389314 17.952481490972747 18-19 25.213502654581276 28.12175029312097 28.807632650186456 17.8571144021113 20-21 24.48676877348633 28.570149157165364 28.750207364140035 18.19287470520827 22-23 25.15279460174891 28.357029826928166 28.15643336169165 18.333742209631275 24-25 23.98526371458627 29.05507155255372 28.50190061971023 18.45776411314978 26-27 23.188474690800398 29.697550442689064 28.288136558445277 18.82583830806526 28-29 23.276102523308907 29.729667231590934 28.111204653823478 18.88302559127668 30-31 23.792168193863542 28.668257718463654 28.42963187637687 19.109942211295934 32-33 22.882530306141774 29.395336701019538 28.70673568113247 19.015397311706216 34-35 23.767666701800447 28.827010849950014 28.704553657266786 18.700768790982757 36-37 23.044308201921258 29.058604405174915 28.916677125978985 18.980410266924842 38-39 22.5889261975401 30.159562354935694 28.296718652135986 18.95479279538822 40-41 23.93882956722101 28.445436220538838 28.213007995569267 19.40272621667089 42-43 23.818219279163593 28.560579199049364 28.281920313812677 19.339281207974366 44-45 22.718857200162116 28.89052053947521 29.091040034594684 19.299582225767992 46-47 22.919959521050203 28.938967250127455 28.49475933041071 19.646313898411634 48-49 21.8980624948892 29.550306903671604 28.699549993867034 19.852080607572166 50-51 21.394360426996926 28.673991983625445 29.52613307756749 20.40551451181014 52-53 22.80492747688948 28.68212127183814 28.32079127851027 20.192159972762113 54-55 23.11053667259256 28.622511655773785 28.595301410158203 19.671650261475452 56-57 23.49521577405192 28.344853930832976 28.18047701086648 19.979453284248624 58-59 22.992081976336152 28.707334930724276 28.437210319858774 19.863372773080794 60-61 21.862410099077206 28.565507836341485 28.495916391922282 21.076165672659023 62-63 22.376960193292454 28.80181760802184 28.109216111046102 20.712006087639597 64-65 21.6896490893373 29.025510688722346 29.033764405089848 20.251075816850513 66-67 21.45261010889179 28.743778963576013 28.55258722783639 21.25102369969581 68-69 21.18073987625357 28.776672895763866 29.811378892221185 20.231208335761377 70-71 20.86663913718541 29.556638608836455 29.467074681885247 20.109647572092896 72-73 20.73536838650815 31.032230507409743 28.046594123922823 20.185806982159285 74-75 20.733628588496984 30.971930436653434 28.308521163615403 19.985919811234176 76-77 21.088509749248047 30.588051275626043 28.12429622902453 20.19914274610138 78-79 20.90036106384541 30.737738674056082 28.40343377782602 19.958466484272495 80-81 20.498934498943118 30.647367121266615 28.583013698980025 20.270684680810238 82-83 20.492413960484253 30.727499752507658 28.250708147447956 20.529378139560134 84-85 20.401266026500725 30.36587616646358 28.680363433394383 20.55249437364131 86-87 20.65182248354246 30.011979424703416 28.733407746375317 20.602790345378803 88-89 20.774504296876124 30.480628690569674 28.46966639533432 20.275200617219884 90-91 20.983546148570024 29.860700431846542 28.448322108218367 20.707431311365063 92-93 20.83202834045452 30.19656145949765 28.15604745672452 20.815362743323305 94-95 20.446579857854893 30.289996767615495 28.602803794260723 20.660619580268886 96-97 20.80877430750851 30.235528767487207 28.17514896663304 20.780547958371244 98-99 20.95379527256825 30.144430587370852 27.998320971869255 20.903453168191646 100-101 21.008587984166827 29.799029323239 28.04649745100353 21.14588524159064 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 826.0 1 672.5 2 391.5 3 283.0 4 315.5 5 354.0 6 453.0 7 641.0 8 873.5 9 1083.5 10 1227.0 11 1286.0 12 1261.0 13 1175.5 14 1059.0 15 928.5 16 885.5 17 949.0 18 1109.5 19 1379.0 20 1719.0 21 2378.5 22 3340.5 23 4664.5 24 6525.0 25 8809.0 26 11827.0 27 15584.0 28 20096.0 29 25401.5 30 31419.0 31 38302.0 32 46951.0 33 57883.5 34 70727.0 35 87137.5 36 107810.5 37 131520.0 38 154720.5 39 170082.5 40 175885.0 41 171365.0 42 161962.5 43 157425.5 44 153499.0 45 149911.5 46 149137.5 47 141645.5 48 125355.0 49 110114.5 50 98870.0 51 89120.0 52 79014.0 53 67815.0 54 57264.0 55 47299.5 56 38204.0 57 30808.0 58 24906.0 59 20355.5 60 16440.5 61 13063.0 62 10263.5 63 7981.0 64 6165.0 65 4791.0 66 3851.0 67 3086.5 68 2457.5 69 1944.5 70 1518.5 71 1200.0 72 972.0 73 801.0 74 630.5 75 522.0 76 415.5 77 317.5 78 261.5 79 194.5 80 159.5 81 130.0 82 94.5 83 97.0 84 90.5 85 64.5 86 45.5 87 41.5 88 40.5 89 32.5 90 20.5 91 15.0 92 20.0 93 18.0 94 12.5 95 12.0 96 11.5 97 8.0 98 4.5 99 4.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2922584148840739 2 0.26781070084083586 3 0.23928836779039142 4 0.22709634372976617 5 0.2328899426306377 6 0.18281669498739095 7 0.14735477644029824 8 0.14519013509271986 9 0.12453054340774396 10-11 0.11066728889494422 12-13 0.13018089398414337 14-15 0.13363477025197062 16-17 0.12765017358513628 18-19 0.11507615399258546 20-21 0.12101300121792907 22-23 0.15128614712303248 24-25 0.5628067503703765 26-27 0.22201898645125515 28-29 0.19639345285124649 30-31 0.29598287132034845 32-33 0.32682901052334024 34-35 0.3345007541228459 36-37 0.32523736247365026 38-39 0.3329409390341498 40-41 0.33548757591365375 42-43 0.3459606200806138 44-45 0.32727467197725346 46-47 0.346406281534527 48-49 0.34296832174719666 50-51 0.3479501801427263 52-53 0.3331319368001126 54-55 0.32547610968110374 56-57 0.33373676305899475 58-59 0.32630376666694255 60-61 0.30392519508830146 62-63 0.29125567661276924 64-65 0.30163322189674785 66-67 0.3178998649645795 68-69 0.3052940124110348 70-71 0.28644889950270547 72-73 0.28331335284481624 74-75 0.27463887097400586 76-77 0.2775993363464292 78-79 0.2861146534122706 80-81 0.29615795260581435 82-83 0.31794761440607017 84-85 0.3090980512497939 86-87 0.31127860907786914 88-89 0.3163241333953864 90-91 0.3097347104696699 92-93 0.2927677422599747 94-95 0.28762671905947607 96-97 0.3047210191131464 98-99 0.31274292528358394 100-101 0.31423907445029253 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3141398.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.45878477575492 #Duplication Level Percentage of deduplicated Percentage of total 1 94.62402932299982 76.13334409913965 2 3.375160536771581 5.431226304234523 3 0.7611647792890394 1.8372717946710546 4 0.327728426254854 1.0547452365174466 5 0.19536751274683326 0.7859516330136006 6 0.12990557255782587 0.6271226702160795 7 0.09837609058920391 0.5540654489857849 8 0.05363016259835181 0.34520141679916233 9 0.03920957470800785 0.2839279259322438 >10 0.32922330636004266 5.061796386933826 >50 0.034747193586043286 1.9996504214746704 >100 0.029505081596293167 4.5961103950808635 >500 0.0014973819091690641 0.818416662078202 >1k 4.5505803304346206E-4 0.47116960492282745 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001273318439751983 0.0 0.0 2 0.001432483244720981 0.0 0.0 3 0.0016553139716775779 0.0 0.0 4 0.0017826458156527762 0.0 0.0 5 0.002100975425590772 0.0 0.0 6 0.0025148039185101663 0.0 0.0 7 0.003151463138386158 0.0 0.0 8 0.0037881223582621497 0.0 0.0 9 0.004233783812175344 0.0 0.0 10-11 0.005745849459380823 0.0 0.0 12-13 0.0071464997431080046 0.0 0.0 14-15 0.008483484104847588 0.0 0.0 16-17 0.00974088606410267 0.0 0.0 18-19 0.011332534113792649 0.0 3.1832960993799575E-5 20-21 0.013338010656402022 3.1832960993799575E-5 6.366592198759915E-5 22-23 0.0150729070305641 3.1832960993799575E-5 6.366592198759915E-5 24-25 0.016632722119260278 3.1832960993799575E-5 6.366592198759915E-5 26-27 0.018335785532428553 3.1832960993799575E-5 6.366592198759915E-5 28-29 0.02042084447752243 3.1832960993799575E-5 6.366592198759915E-5 30-31 0.03687848531131681 3.1832960993799575E-5 6.366592198759915E-5 32-33 0.03958428699578977 3.1832960993799575E-5 6.366592198759915E-5 34-35 0.04195584258982784 3.1832960993799575E-5 9.549888298139873E-5 36-37 0.044773059637779104 3.1832960993799575E-5 9.549888298139873E-5 38-39 0.04727194707579237 3.1832960993799575E-5 9.549888298139873E-5 40-41 0.050264245409209526 3.1832960993799575E-5 9.549888298139873E-5 42-43 0.05417969961144688 3.1832960993799575E-5 9.549888298139873E-5 44-45 0.058397566943125326 6.366592198759915E-5 9.549888298139873E-5 46-47 0.06314067813120146 6.366592198759915E-5 1.273318439751983E-4 48-49 0.06907752535654507 6.366592198759915E-5 1.5916480496899787E-4 50-51 0.07627177454114378 6.366592198759915E-5 1.5916480496899787E-4 52-53 0.08400718406263707 6.366592198759915E-5 1.5916480496899787E-4 54-55 0.09120143324723579 6.366592198759915E-5 1.7508128546589766E-4 56-57 0.09976449975456787 6.366592198759915E-5 1.9099776596279746E-4 58-59 0.10977596598711784 6.366592198759915E-5 1.9099776596279746E-4 60-61 0.12168149339879888 6.366592198759915E-5 2.2283072695659703E-4 62-63 0.1338576009789272 6.366592198759915E-5 2.705801684472964E-4 64-65 0.1483893476725967 6.366592198759915E-5 2.864966489441962E-4 66-67 0.1646082412989376 6.366592198759915E-5 3.0241312944109594E-4 68-69 0.18498133633496933 6.366592198759915E-5 3.1832960993799573E-4 70-71 0.20517935008553517 6.366592198759915E-5 3.1832960993799573E-4 72-73 0.22819458088405226 6.366592198759915E-5 3.1832960993799573E-4 74-75 0.25402702873052063 6.366592198759915E-5 3.660790514286951E-4 76-77 0.2830905221178596 6.366592198759915E-5 3.819955319255949E-4 78-79 0.3160376367464422 6.366592198759915E-5 3.819955319255949E-4 80-81 0.3521521309939078 6.366592198759915E-5 3.979120124224947E-4 82-83 0.3937578110128038 6.366592198759915E-5 4.4566145391319406E-4 84-85 0.44061592959567686 6.366592198759915E-5 4.7749441490699366E-4 86-87 0.4933631459624027 6.366592198759915E-5 4.7749441490699366E-4 88-89 0.5498507352459001 6.366592198759915E-5 4.7749441490699366E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1360 0.0 77.84949 2 GAGCGGC 965 0.0 73.708984 9 AGAGCGG 1250 0.0 61.45951 8 GATCGGG 1790 0.0 60.490818 1 GTTCGGA 370 0.0 60.30953 2 AAGCGGC 405 0.0 59.71337 9 CGGGAGA 1665 0.0 57.01118 4 TTGTGTT 30265 0.0 56.61526 1 GAGAGCG 1645 0.0 53.620533 7 TCGGGAG 1775 0.0 52.945 3 TCGGAAA 620 0.0 52.820396 4 GATCCGG 365 0.0 52.044487 1 TCGGAGA 560 0.0 51.699467 4 TTCGGAA 430 0.0 50.774715 3 TTCGGAG 465 0.0 48.994396 3 GGTTCGG 555 0.0 46.207066 1 GGATCGG 600 0.0 44.324554 1 AAAGCGG 560 0.0 44.035145 8 TCGGGAA 650 0.0 43.812294 3 CGGAAAG 1085 0.0 42.86873 5 >>END_MODULE