FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_50330665-2.34200000035755.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_50330665-2.34200000035755.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2579697
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG83040.32189826944792355No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC81070.31426171368187816No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA41910.16246094018018395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC4500.060.014579
GTTCGGA1300.054.7530982
AGAGCGG5150.054.282028
CGGGAGA6800.051.6146054
TTGTGTT347950.045.637361
TCGGGAG8000.043.874973
CGACGGG554.9908835E-543.1438371
GGTTCGG2450.042.6155431
CGGAAAG3150.040.6539735
GAGAGCG7300.039.5945137
CGGAGAG4450.039.435885
TTCGGAG1700.039.0619773
TCGCCGG651.3364549E-436.5020642
TGTGTTT578250.035.364891
GTCGGGA1750.035.2505652
TCGGAGA2200.034.4942784
AGCGCGC550.002611945834.459468
CGGACGC702.0646807E-433.898731
CGCGGGT702.0712918E-433.8802873
GGTACGG851.6428492E-533.4999241
Produced by FastQC (version 0.11.2)