##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_50330665-1.342000000356cf.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2912488 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.09501635714894 33.0 33.0 33.0 22.0 33.0 2 29.184570717544588 33.0 33.0 33.0 15.0 33.0 3 28.912861443549296 33.0 33.0 33.0 15.0 33.0 4 32.022952884269394 37.0 37.0 37.0 6.0 37.0 5 32.14148350139125 37.0 37.0 37.0 6.0 37.0 6 33.066090229384635 37.0 37.0 37.0 15.0 37.0 7 32.3872064022238 37.0 37.0 37.0 15.0 37.0 8 32.58363227590981 37.0 33.0 37.0 15.0 37.0 9 31.728028407327344 37.0 33.0 37.0 6.0 37.0 10-11 31.842982014003148 37.0 33.0 37.0 6.0 37.0 12-13 31.809195780377465 37.0 33.0 37.0 10.5 37.0 14-15 33.26734839765864 37.0 33.0 40.0 10.5 40.0 16-17 33.079022299834364 37.0 33.0 40.0 6.0 40.0 18-19 33.249679655332486 37.0 33.0 40.0 10.5 40.0 20-21 33.197199782454035 37.0 33.0 40.0 10.5 40.0 22-23 34.52583358283364 37.0 33.0 40.0 18.5 40.0 24-25 33.60697314461038 37.0 33.0 40.0 15.0 40.0 26-27 33.156615409230874 37.0 33.0 40.0 10.5 40.0 28-29 32.79748517418784 37.0 33.0 40.0 6.0 40.0 30-31 32.84204295434007 37.0 33.0 40.0 10.5 40.0 32-33 32.55998531152747 37.0 33.0 40.0 6.0 40.0 34-35 32.864892490544165 37.0 33.0 40.0 10.5 40.0 36-37 32.68733227398705 37.0 33.0 40.0 6.0 40.0 38-39 32.456141621871055 37.0 33.0 40.0 6.0 40.0 40-41 32.251300777891615 37.0 33.0 40.0 6.0 40.0 42-43 32.15929439022581 37.0 33.0 40.0 6.0 40.0 44-45 32.00308602129863 37.0 33.0 40.0 6.0 40.0 46-47 31.903799946986908 37.0 33.0 40.0 6.0 40.0 48-49 31.80175677977042 37.0 33.0 40.0 6.0 40.0 50-51 30.993798772733143 35.0 30.0 38.5 6.0 38.5 52-53 31.390545300100804 37.0 33.0 38.5 6.0 40.0 54-55 31.964365003392288 37.0 33.0 40.0 6.0 40.0 56-57 32.445415912443245 37.0 33.0 40.0 10.5 40.0 58-59 31.71184979989617 37.0 33.0 40.0 6.0 40.0 60-61 31.46710801898583 37.0 33.0 40.0 6.0 40.0 62-63 31.024360615391377 37.0 33.0 37.0 6.0 40.0 64-65 30.812625150730234 37.0 33.0 37.0 6.0 40.0 66-67 30.648364216436256 37.0 27.0 37.0 6.0 40.0 68-69 30.90535858001818 37.0 27.0 37.0 10.5 38.5 70-71 30.528695740548976 37.0 27.0 37.0 6.0 37.0 72-73 31.0185226514238 37.0 27.0 37.0 15.0 37.0 74-75 31.444891961786624 37.0 27.0 37.0 15.0 37.0 76-77 31.20671793325844 33.0 27.0 37.0 22.0 37.0 78-79 30.765208646353223 33.0 27.0 37.0 15.0 37.0 80-81 30.09855233738302 33.0 27.0 37.0 15.0 37.0 82-83 29.56064454171142 33.0 27.0 37.0 6.0 37.0 84-85 29.21359401309121 33.0 27.0 37.0 6.0 37.0 86-87 29.000367898511513 33.0 27.0 37.0 6.0 37.0 88-89 28.90627686706349 33.0 27.0 37.0 6.0 37.0 90-91 28.797576505036247 33.0 27.0 37.0 6.0 37.0 92-93 28.573552406052833 33.0 24.5 37.0 6.0 37.0 94-95 28.36837353492959 33.0 22.0 37.0 6.0 37.0 96-97 28.219285023663616 33.0 22.0 37.0 6.0 37.0 98-99 28.091398659839975 33.0 22.0 37.0 6.0 37.0 100-101 26.84066852807634 33.0 18.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.12842622789710134 2306 2 0.12060890973476646 2306 3 0.039244435206803985 2306 4 0.1639890658122951 2306 5 0.0472192985085087 2306 6 -0.49890922331272947 2306 7 0.2761326277925704 2306 8 -0.2970825056242745 2306 9 0.20913851669964956 2306 10-11 0.1310836640810713 2306 12-13 0.11837427209329121 2306 14-15 0.568323574323891 2306 16-17 0.5360583628124189 2306 18-19 0.48876506614545434 2306 20-21 0.5189204362393696 2306 22-23 -0.052394886297442156 2306 24-25 0.5197254830303351 2306 26-27 -0.07745260062270631 2306 28-29 0.0449402573192188 2306 30-31 0.12333896778210374 2306 32-33 0.32725434137132225 2306 34-35 -0.012640520851100234 2306 36-37 0.28874266350457845 2306 38-39 0.14047962001262704 2306 40-41 0.2554462668675228 2306 42-43 0.3229354608915642 2306 44-45 0.4500939456286872 2306 46-47 0.38010169600773125 2306 48-49 0.3031828219844819 2306 50-51 0.24498572767316773 2306 52-53 0.23838224739670721 2306 54-55 0.30980309480679935 2306 56-57 0.008372346508728867 2306 58-59 0.6175841618908997 2306 60-61 0.45965861667446717 2306 62-63 0.41494231810158055 2306 64-65 0.3159207224431988 2306 66-67 0.29335727686144253 2306 68-69 -0.0840812281206631 2306 70-71 0.16786090279538257 2306 72-73 -0.32424317406577075 2306 74-75 -0.46768897877311133 2306 76-77 -0.07594146369450172 2306 78-79 0.2076453063662136 2306 80-81 0.5592407011186431 2306 82-83 0.7390004214737438 2306 84-85 0.7152181350371656 2306 86-87 0.6590345711304657 2306 88-89 0.5064193533507826 2306 90-91 0.3519422544591819 2306 92-93 0.23318831121586925 2306 94-95 0.17377650829028113 2306 96-97 0.06630933695282337 2306 98-99 0.2913780286007075 2306 100-101 0.2578697422021179 2307 1 -0.23025960219053943 2307 2 -0.0668797677219608 2307 3 -0.3254994471089603 2307 4 -0.07374151032083631 2307 5 -0.31313319245100857 2307 6 -0.5776509288914937 2307 7 0.07219062730104753 2307 8 -0.605475582196263 2307 9 0.023940328306572667 2307 10-11 -0.25290327549692293 2307 12-13 -0.15311538840595418 2307 14-15 0.26581400721368453 2307 16-17 0.11325950033303656 2307 18-19 0.039083501703508716 2307 20-21 0.010976750532101676 2307 22-23 -0.2549704576909093 2307 24-25 0.14368293133284027 2307 26-27 -0.4336861434377184 2307 28-29 -0.2593057841792188 2307 30-31 -0.2114495025335259 2307 32-33 0.07511165977177114 2307 34-35 -0.28397991627995367 2307 36-37 0.2161906149141899 2307 38-39 -0.16080254829829954 2307 40-41 -0.060706526026486074 2307 42-43 -0.07528242346050718 2307 44-45 -0.06207401420700975 2307 46-47 -0.11035366332941976 2307 48-49 -0.07695620831429295 2307 50-51 -0.36693928022627986 2307 52-53 -0.3829567969455354 2307 54-55 -0.11359682795414372 2307 56-57 -0.44589581344632734 2307 58-59 0.13387387856400323 2307 60-61 0.06541573495554687 2307 62-63 0.14480293671619293 2307 64-65 0.12415370351962807 2307 66-67 0.03922252981934804 2307 68-69 -0.27086211496544266 2307 70-71 -0.12104405805846241 2307 72-73 -0.6077287619603027 2307 74-75 -0.5377174167887695 2307 76-77 -0.2759049506867406 2307 78-79 -0.014547580619002076 2307 80-81 0.21416469086691237 2307 82-83 0.3268766985512954 2307 84-85 0.4435908485856004 2307 86-87 0.23160979143731808 2307 88-89 0.11453507149845166 2307 90-91 0.20335678242322786 2307 92-93 0.10995480350952036 2307 94-95 0.1960648908342577 2307 96-97 -0.1048804955788647 2307 98-99 0.004281865977389998 2307 100-101 -0.047529969906861425 2201 1 -0.059839079317185906 2201 2 -0.04113494650544425 2201 3 -0.25145085419244495 2201 4 -0.1327112198565814 2201 5 -0.15868443366262852 2201 6 -0.4204581216986867 2201 7 0.19924432831701466 2201 8 -0.4858525637053219 2201 9 -0.11653711877464801 2201 10-11 -0.18341191779802202 2201 12-13 -0.21745077000548463 2201 14-15 0.27906641518307396 2201 16-17 0.05103219933214831 2201 18-19 0.055029442555103 2201 20-21 -0.012320047009978907 2201 22-23 -0.14482616801831227 2201 24-25 0.19519029189680026 2201 26-27 -0.4077526429759146 2201 28-29 -0.19960011249241205 2201 30-31 -0.02769259905964816 2201 32-33 0.03947474951221608 2201 34-35 -0.26856683879140775 2201 36-37 0.17149003247775596 2201 38-39 -0.11050199979019482 2201 40-41 -0.11580369301044158 2201 42-43 -0.09247202631048879 2201 44-45 -0.06374394804675632 2201 46-47 0.050940042964285226 2201 48-49 -0.039071922788497915 2201 50-51 -0.14886047224181453 2201 52-53 -0.12569330571977844 2201 54-55 0.10240085360957707 2201 56-57 -0.019158022934959718 2201 58-59 0.29700216537153423 2201 60-61 0.17873910969401763 2201 62-63 0.19235359360647308 2201 64-65 0.05616226099988708 2201 66-67 0.0997411621304316 2201 68-69 -0.15054083247435202 2201 70-71 -0.0646060620921638 2201 72-73 -0.4598211035809463 2201 74-75 -0.3207165874294482 2201 76-77 -0.1729779720050466 2201 78-79 0.26133964536256116 2201 80-81 0.47015021477696806 2201 82-83 0.3196281923405273 2201 84-85 0.2920260925868803 2201 86-87 0.24343645533945235 2201 88-89 0.1317068619721269 2201 90-91 0.018943271405944984 2201 92-93 0.141601647231834 2201 94-95 0.18933925797654894 2201 96-97 0.17277109811046287 2201 98-99 0.16760693713264985 2201 100-101 0.16600555117582871 1101 1 -0.07776311269487479 1101 2 -0.127389628841879 1101 3 -0.1696738217862226 1101 4 0.0677366158398982 1101 5 -0.04701504019930525 1101 6 -0.024913641446872248 1101 7 0.3481967151950158 1101 8 -0.09964624253345278 1101 9 0.012054440081030293 1101 10-11 0.03380165189225792 1101 12-13 0.013919752874858915 1101 14-15 0.40471564967674567 1101 16-17 0.25790432788800643 1101 18-19 0.20367886511997568 1101 20-21 0.15401743614492602 1101 22-23 0.1696099577716197 1101 24-25 0.1735341765426952 1101 26-27 -0.23289344891048103 1101 28-29 -0.07615629558042514 1101 30-31 -0.0126912698346473 1101 32-33 -0.006904773918201101 1101 34-35 -0.18777117036798785 1101 36-37 0.0850925291582314 1101 38-39 -0.14544311795123122 1101 40-41 -0.009636796086191168 1101 42-43 0.056632726517300114 1101 44-45 0.10503351140834383 1101 46-47 0.047932848180504806 1101 48-49 0.04058453618440794 1101 50-51 -0.07793907186086457 1101 52-53 -0.21388773739832345 1101 54-55 0.12756757236112648 1101 56-57 0.19880871533074185 1101 58-59 0.44295018560403676 1101 60-61 0.2453797692724251 1101 62-63 0.16028657097146493 1101 64-65 0.13849785071620957 1101 66-67 0.168932448919211 1101 68-69 0.06899393170840895 1101 70-71 0.12986079142017104 1101 72-73 -0.5248942675182242 1101 74-75 -0.21628775823627322 1101 76-77 0.10227429792944065 1101 78-79 0.2630968030270253 1101 80-81 0.4200822687548147 1101 82-83 0.3292889359429232 1101 84-85 0.18204704202118194 1101 86-87 0.09103868900306367 1101 88-89 0.17079862657486444 1101 90-91 0.12265286968304423 1101 92-93 0.16202858298643008 1101 94-95 0.11091056453400938 1101 96-97 0.10208072127518264 1101 98-99 0.13732871195911045 1101 100-101 0.12024450623529859 2304 1 -0.022127734058670967 2304 2 -0.20482848567068146 2304 3 -0.18209285370236827 2304 4 -0.07057911715844156 2304 5 -0.21351780783562546 2304 6 -0.10689390813447375 2304 7 0.25111614622656475 2304 8 -0.16447436119504033 2304 9 0.012798692164935233 2304 10-11 0.007032621938972028 2304 12-13 -0.0037167315072963447 2304 14-15 0.19519740159708476 2304 16-17 0.18029551986906256 2304 18-19 0.1118734894934974 2304 20-21 0.011120218175570074 2304 22-23 -0.036435659156104805 2304 24-25 0.0671841523340575 2304 26-27 -0.020484067735644373 2304 28-29 0.06287980800637882 2304 30-31 0.23095281736879514 2304 32-33 0.18291385757397194 2304 34-35 -0.06141581371584692 2304 36-37 0.24303737926095437 2304 38-39 0.050000260004516406 2304 40-41 0.1364271736072098 2304 42-43 0.16306617238809196 2304 44-45 0.22332281118981712 2304 46-47 0.24653888856313344 2304 48-49 0.1492190053609228 2304 50-51 0.21150089071388933 2304 52-53 0.11302916154042464 2304 54-55 0.21752344066612395 2304 56-57 0.3265065064559849 2304 58-59 0.23475605194617444 2304 60-61 0.09851707805689003 2304 62-63 0.26711978653304413 2304 64-65 0.37123856310448744 2304 66-67 0.371453909550727 2304 68-69 0.2699681658648352 2304 70-71 0.38739916538476393 2304 72-73 0.14187136013982027 2304 74-75 0.4142789202075683 2304 76-77 0.44389663661484846 2304 78-79 0.5968657282943042 2304 80-81 0.5568844710866934 2304 82-83 0.444379751054349 2304 84-85 0.4491219841167329 2304 86-87 0.3676690099965363 2304 88-89 0.45062481508819374 2304 90-91 0.39858572015216964 2304 92-93 0.4801379536926689 2304 94-95 0.42999956726893274 2304 96-97 0.504918039219671 2304 98-99 0.5134760051715297 2304 100-101 0.5703576979808105 2202 1 -0.10230112267533187 2202 2 -0.40933910258235073 2202 3 -0.26318972516863326 2202 4 -0.2150549725841664 2202 5 -0.32967875770370014 2202 6 -0.025956185640026774 2202 7 0.1906353488064525 2202 8 -0.04840869130351422 2202 9 -0.13944509893109824 2202 10-11 -0.11972168136546202 2202 12-13 -0.2050386103027897 2202 14-15 0.004094038528016597 2202 16-17 -0.07684053156118864 2202 18-19 -0.17619557278401743 2202 20-21 -0.14076496421752438 2202 22-23 0.06757238205509708 2202 24-25 -0.18814576767264413 2202 26-27 -0.09870107573497222 2202 28-29 -0.2818138302228803 2202 30-31 0.0033650760277481595 2202 32-33 -0.11401414845693836 2202 34-35 -0.06470768455389475 2202 36-37 0.06557737591045054 2202 38-39 -0.1518472292110502 2202 40-41 -0.15243606298029988 2202 42-43 -0.030137014779477056 2202 44-45 -0.015228933166675063 2202 46-47 -0.08006516554751997 2202 48-49 -0.04180007157504306 2202 50-51 0.08574650364683833 2202 52-53 -0.11819701846543751 2202 54-55 0.08890541913913452 2202 56-57 0.2867250221824236 2202 58-59 0.10102746376508165 2202 60-61 0.036095081993732236 2202 62-63 0.019778726098316213 2202 64-65 0.06856496508128629 2202 66-67 0.22066182930614175 2202 68-69 0.11867193937071718 2202 70-71 0.13264668391724044 2202 72-73 0.07036164367627507 2202 74-75 0.5411761434768216 2202 76-77 0.36260239948287776 2202 78-79 0.3587238972435003 2202 80-81 0.17900565465246743 2202 82-83 0.11793065778467238 2202 84-85 0.2601723673307035 2202 86-87 0.26253907323730985 2202 88-89 0.15532546843660455 2202 90-91 0.11177047954404884 2202 92-93 0.05142831680361226 2202 94-95 0.11964284322487373 2202 96-97 0.14451786331817473 2202 98-99 0.2023125424249521 2202 100-101 0.2780115070858038 1102 1 -0.15535960138311822 1102 2 -0.6031630435937743 1102 3 -0.4623857055969829 1102 4 -0.6474699420654311 1102 5 -0.5403074583027205 1102 6 -0.05550254090915985 1102 7 -0.22954000063090518 1102 8 -0.07465128525880971 1102 9 -0.6274270405479392 1102 10-11 -0.5267629940296779 1102 12-13 -0.4684985504312884 1102 14-15 -0.49711596509174427 1102 16-17 -0.5524274137814942 1102 18-19 -0.6336570494426326 1102 20-21 -0.7234463062054317 1102 22-23 -0.27220592611667627 1102 24-25 -0.5295612088032939 1102 26-27 -0.306214827097925 1102 28-29 -0.5033734138309782 1102 30-31 -0.35496542085473237 1102 32-33 -0.6468069359601927 1102 34-35 -0.5137635272318235 1102 36-37 -0.5022250619878932 1102 38-39 -0.5823515063204354 1102 40-41 -0.6081562034614691 1102 42-43 -0.6275918113540229 1102 44-45 -0.49137809733915816 1102 46-47 -0.4838522220814703 1102 48-49 -0.4410832642121072 1102 50-51 -0.3685652662901795 1102 52-53 -0.4915392871959128 1102 54-55 -0.33153289629432336 1102 56-57 -0.0704225431457246 1102 58-59 -0.4571513049226077 1102 60-61 -0.6046750192571757 1102 62-63 -0.46454329383503534 1102 64-65 -0.48933387429564945 1102 66-67 -0.27138909221035235 1102 68-69 -0.296766144807755 1102 70-71 -0.2823438457065208 1102 72-73 -0.05340186916169998 1102 74-75 0.3345677484501053 1102 76-77 0.12554776265428913 1102 78-79 0.07956825211549301 1102 80-81 -0.07526494635846248 1102 82-83 -0.18134334004346542 1102 84-85 -0.13001800398725294 1102 86-87 -0.23496108808727456 1102 88-89 -0.28618329169603385 1102 90-91 -0.2554060051198661 1102 92-93 -0.16771901422307778 1102 94-95 -0.1936532382079008 1102 96-97 -0.2169102732956354 1102 98-99 -0.20421931689557837 1102 100-101 -0.38377150207050903 2305 1 0.3219050411461737 2305 2 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0.4851131025295743 1213 68-69 0.6024872786215276 1213 70-71 0.4432821233195554 1213 72-73 0.9106777127744614 1213 74-75 0.7889073785023264 1213 76-77 0.44603503697139146 1213 78-79 0.43394961872592575 1213 80-81 0.30625966392841164 1213 82-83 0.4060342472660814 1213 84-85 0.39445650021259127 1213 86-87 0.37130943304840613 1213 88-89 0.42418493027897597 1213 90-91 0.45217386167227147 1213 92-93 0.5687088680195238 1213 94-95 0.6094293557429964 1213 96-97 0.7200548282765453 1213 98-99 0.47361819379643677 1213 100-101 0.5657247828997285 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1418.0 3 626.0 4 592.0 5 428.0 6 1039.0 7 7613.0 8 46468.0 9 129732.0 10 137983.0 11 69456.0 12 32053.0 13 18489.0 14 12776.0 15 10065.0 16 9093.0 17 8139.0 18 7967.0 19 8039.0 20 8593.0 21 9167.0 22 9834.0 23 11212.0 24 12486.0 25 14538.0 26 17619.0 27 21907.0 28 27823.0 29 35349.0 30 42236.0 31 54862.0 32 76996.0 33 111333.0 34 173126.0 35 299837.0 36 551076.0 37 777999.0 38 154459.0 39 60.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.30323189333994 18.920073824625195 21.866482105103678 11.910212176931182 2 36.06398740879154 23.809109072857986 29.96746169554447 10.159441822806006 3 39.943408193697884 18.442100944997815 28.59575988197077 13.01873097933353 4 29.94521867795327 15.016895495681498 35.79780461787275 19.240081208492484 5 37.24667390943013 12.681145081591211 32.425154574039134 17.64702643493953 6 36.043265167694486 12.667746942933963 37.04705530700335 14.2419325823682 7 43.16084245027304 15.523182305669666 25.67925312672893 15.63672211732837 8 30.10709985235432 34.834753056238085 20.002723227271886 15.055423864135717 9 37.401088380183644 28.079521195224327 18.530480489255424 15.988909935336604 10-11 32.38831572302681 30.10280550993124 22.82189859275087 14.686980174291078 12-13 36.676231605484695 21.76950394048778 22.46286889375664 19.09139556027089 14-15 32.29410872845528 21.728466623278976 24.18346392615979 21.79396072210596 16-17 33.725037214275154 23.301310148753966 25.59242032019747 17.38123231677341 18-19 33.488657796581094 23.389897974651955 26.117299760516627 17.00414446825032 20-21 30.64363793305852 26.94785344878409 25.521866852343077 16.886641765814307 22-23 35.50611599632413 23.187728291415254 24.083073360148756 17.223082352111867 24-25 30.787812518449954 27.549856733425425 24.657801943066943 17.004528805057678 26-27 24.0278274598384 32.560547161653126 25.362386542089006 18.04923883641947 28-29 26.37649737448786 30.676450826392117 23.822751181678285 19.12430061744174 30-31 30.302917597776425 25.913055786001276 24.797497617770965 18.986528998451334 32-33 26.837108577918578 27.098345134363637 26.454511648511698 19.610034639206084 34-35 32.82955654333067 23.883925610830577 24.26131465192411 19.025203193914646 36-37 28.22284480278997 25.16795724104719 26.889090521400316 19.720107434762525 38-39 21.075619575554207 29.41376583464056 28.86084635952478 20.649768230280454 40-41 22.814735093462886 31.611363615223464 25.570304643637325 20.00359664767632 42-43 28.60285122699096 26.135276944022714 26.897345250742973 18.36452657824336 44-45 24.51799501911967 28.773307938043345 27.2059445371965 19.50275250564048 46-47 26.869235918905687 30.196471892250038 24.563396752079093 18.370895436765185 48-49 22.014539959565667 28.888600247236507 28.6969023774119 20.399957415785927 50-51 18.93597374518705 25.776300035316513 30.900536638729292 24.387189580767142 52-53 26.769863907038772 25.69512758295256 25.432308612652417 22.102699897356256 54-55 31.198338958266415 25.177872182115212 25.10494755851495 18.51884130110343 56-57 32.96735286067473 24.755537894145572 23.093493567566348 19.183615677613354 58-59 29.83583720407913 24.180942840647447 23.43057390839457 22.552646046878856 60-61 23.355245144921373 23.880384721221073 24.721630908293783 28.04273922556377 62-63 26.61863331536358 23.563712861203577 25.082469439517734 24.735184383915108 64-65 24.56048421813924 27.170543970519862 27.383293441013894 20.885678370327003 66-67 21.544586280696624 26.832781620336384 26.660008566319483 24.96262353264751 68-69 19.541178980699662 23.511214976285068 35.547737158351474 21.399868884663796 70-71 18.72580281663952 30.615902948021173 31.31411441171199 19.344179823627314 72-73 20.461334195530828 37.10116048890455 24.557013325840753 17.880491989723872 74-75 21.457097975081233 36.906147920208305 23.804660971358484 17.832093133351982 76-77 20.625666885992324 36.028163893075124 23.786056603923004 19.560112617009548 78-79 20.509396533111946 36.60149106281699 24.12388610183592 18.765226302235146 80-81 19.653867240070692 37.15307862586902 24.053370240937134 19.139683893123145 82-83 19.53284804282984 36.125863462488674 24.317191026000707 20.024097468680782 84-85 20.298556811812084 34.63878965409463 24.453692007918118 20.608961526175168 86-87 19.33707318543266 34.547536734790526 25.260602825123886 20.85478725465293 88-89 19.089990846520344 34.59836201796769 25.342411610366593 20.969235525145375 90-91 20.064582246615597 33.255999499901755 24.6868746081143 21.992543645368347 92-93 20.781087262367006 33.019189707452995 23.921817580438073 22.277905449741926 94-95 21.14874778884327 34.196010155566945 23.376622705811904 21.278619349777877 96-97 20.519871530276504 34.856134539506364 22.59086119521753 22.033132734999597 98-99 20.706181360150325 32.788362067258205 24.31441307035516 22.19104350223631 100-101 21.865858384066268 33.75606857772688 23.589394735255876 20.788678302950977 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1392.0 1 1009.5 2 508.5 3 560.0 4 601.5 5 465.0 6 593.0 7 777.5 8 933.5 9 1111.0 10 1204.5 11 1256.5 12 1250.0 13 1154.5 14 1023.0 15 914.5 16 832.0 17 751.5 18 699.5 19 687.0 20 681.0 21 714.0 22 772.0 23 842.5 24 975.5 25 1162.5 26 1430.5 27 1772.0 28 2168.0 29 2733.5 30 3459.5 31 4659.5 32 7162.0 33 11418.0 34 17896.5 35 28101.0 36 42187.5 37 61398.5 38 89846.5 39 114176.0 40 125709.0 41 127139.5 42 148972.0 43 188013.5 44 188603.5 45 172059.0 46 178431.5 47 181873.5 48 165728.5 49 143671.0 50 122829.0 51 112222.0 52 107988.0 53 108239.0 54 99234.5 55 70362.5 56 48310.0 57 40667.5 58 36750.5 59 31389.5 60 24398.0 61 18793.0 62 14131.0 63 10429.5 64 8086.0 65 6172.5 66 4679.5 67 3747.5 68 2991.0 69 2315.0 70 1790.5 71 1422.5 72 1081.5 73 808.0 74 630.0 75 494.5 76 380.5 77 276.5 78 212.5 79 185.5 80 152.0 81 117.5 82 90.5 83 66.0 84 47.0 85 42.0 86 48.0 87 33.0 88 24.0 89 20.0 90 10.0 91 9.0 92 7.0 93 6.0 94 6.0 95 3.0 96 1.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2852543941811949 2 0.2610826207695963 3 0.23234430493790878 4 0.21933137578592599 5 0.22657604082832275 6 0.17878185249175274 7 0.14616369234826032 8 0.14331389519888152 9 0.12285029157201677 10-11 0.10848113365617301 12-13 0.12959710048590758 14-15 0.1314683528309816 16-17 0.125597084005153 18-19 0.1136142020155963 20-21 0.11963997791578883 22-23 0.14885898242327522 24-25 0.5541310384798153 26-27 0.2235030667937516 28-29 0.19972614479441633 30-31 0.2985420025764913 32-33 0.32836186792872624 34-35 0.3358125424036082 36-37 0.3267652948269658 38-39 0.3335464386462708 40-41 0.3359670494779721 42-43 0.3475894149606797 44-45 0.3291859056586671 46-47 0.3481216059945998 48-49 0.34348639376368245 50-51 0.3489456437245407 52-53 0.33406146222748384 54-55 0.3258554198334894 56-57 0.33433614147079743 58-59 0.3267309599215516 60-61 0.30485962517270454 62-63 0.2929969153520976 64-65 0.30345189405072226 66-67 0.31785538687198023 68-69 0.3068682171394354 70-71 0.2868166323775411 72-73 0.28432735173501145 74-75 0.27641315603703775 76-77 0.279177115922881 78-79 0.28789818189808847 80-81 0.2973402808869942 82-83 0.3185764198856785 84-85 0.30971801428881424 86-87 0.3116064340865954 88-89 0.31658499537165474 90-91 0.31102274069455393 92-93 0.2939582927036952 94-95 0.2906449743312247 96-97 0.3064218633690508 98-99 0.31431889161431736 100-101 0.31632748358104823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2912488.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.58599247331996 #Duplication Level Percentage of deduplicated Percentage of total 1 86.44010471125294 35.082574392039014 2 5.390465505045206 4.375547848309112 3 2.2648014110103905 2.757576390624964 4 1.216210490518021 1.9744443925654869 5 0.7672156590181293 1.5569104481161504 6 0.6577071313248016 1.6016218008958356 7 0.4383674030221851 1.2454103283725058 8 0.29629162006076243 0.9620231569355104 9 0.2522074960840558 0.9212482384004126 >10 1.8551063341412315 14.937027608057212 >50 0.21409982360359364 6.19454884628934 >100 0.18293754924655137 15.304296895481006 >500 0.017031190291511292 4.557798463009556 >1k 0.006690054486865211 4.737957170890044 >5k 2.545402980161827E-4 0.7240837126440741 >10k+ 5.090805956463323E-4 3.066930307369998 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 22409 0.7694108954268652 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 20811 0.7145437165749695 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 13624 0.4677787513630957 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11817 0.40573557727963167 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 10287 0.3532031719959018 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 10114 0.34726323335924475 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 8033 0.2758122951922892 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 6956 0.23883360206119306 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6038 0.2073141588909551 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 4665 0.16017233375725495 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 4365 0.1498718621329942 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 4246 0.1457860083887041 No Hit GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3770 0.12944259341154368 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3317 0.1138888812589099 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 3135 0.10763992847352503 No Hit TTGTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGA 2986 0.10252402756680885 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.007965698056094995 0.0 0.0 2 0.008927075407692667 0.0 0.0 3 0.00951076879973411 0.0 0.0 4 0.00978544804304773 0.0 0.0 5 0.011502193313757859 0.0 0.0 6 0.012360565949112924 0.0 0.0 7 0.013905636692752039 0.0 0.0 8 0.015313367814734344 0.0 0.0 9 0.017201787612515486 0.0 0.0 10-11 0.02609452811479395 0.0 0.0 12-13 0.03395722145464634 0.0 0.0 14-15 0.04169974262554901 0.0 0.0 16-17 0.05045514350617067 0.0 0.0 18-19 0.05811182741353785 0.0 0.0 20-21 0.06367408209063866 0.0 0.0 22-23 0.06875564809194062 0.0 0.0 24-25 0.07450674474881958 0.0 0.0 26-27 0.08080719989232574 0.0 0.0 28-29 0.0884123814415716 0.0 0.0 30-31 0.16764017568484402 0.0 0.0 32-33 0.17933121097838 0.0 0.0 34-35 0.18803510950088037 0.0 0.0 36-37 0.19790639480746358 0.0 0.0 38-39 0.2102154583984552 0.0 0.0 40-41 0.22525414696987592 0.0 0.0 42-43 0.24149455723079374 1.716745270710128E-5 0.0 44-45 0.2585246703162382 3.433490541420256E-5 0.0 46-47 0.2773917008413425 3.433490541420256E-5 0.0 48-49 0.298833849272512 3.433490541420256E-5 0.0 50-51 0.3212202076025721 3.433490541420256E-5 0.0 52-53 0.3453061437506352 3.433490541420256E-5 0.0 54-55 0.37364960817005943 3.433490541420256E-5 0.0 56-57 0.40468836266449854 3.433490541420256E-5 0.0 58-59 0.4393322822274289 3.433490541420256E-5 0.0 60-61 0.4749204116892499 3.433490541420256E-5 0.0 62-63 0.5141652085776833 3.433490541420256E-5 0.0 64-65 0.556465812047981 3.433490541420256E-5 0.0 66-67 0.6014788730460006 3.433490541420256E-5 0.0 68-69 0.6487752052540645 3.433490541420256E-5 0.0 70-71 0.7043119147615373 3.433490541420256E-5 0.0 72-73 0.763264947357723 3.433490541420256E-5 0.0 74-75 0.8276600624620599 3.433490541420256E-5 0.0 76-77 0.900226885054977 3.433490541420256E-5 0.0 78-79 0.9811714245689596 3.433490541420256E-5 0.0 80-81 1.0723821008017886 3.433490541420256E-5 0.0 82-83 1.1739104161115856 3.433490541420256E-5 0.0 84-85 1.2923315048851705 3.433490541420256E-5 0.0 86-87 1.422958652533504 3.433490541420256E-5 0.0 88-89 1.5664785571648707 3.433490541420256E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCGGA 1230 0.0 89.529915 2 ATTCGGA 1035 0.0 88.970634 2 GAGCGGC 3555 0.0 87.76539 9 AAGCGGC 1325 0.0 86.245834 9 CGGGAGA 4835 0.0 85.58559 4 TCGGAGA 1690 0.0 84.52018 4 ATCCGGA 1220 0.0 83.26054 2 CGGAGAG 2665 0.0 82.801155 5 CGGAAAG 3665 0.0 82.479515 5 GGATCGG 2910 0.0 81.57405 1 AGAGCGG 4035 0.0 81.20289 8 ATCGGGA 6300 0.0 80.39132 2 ACCGGAA 910 0.0 80.31236 3 TCGGGAG 5250 0.0 78.28195 3 GTCCGGA 530 0.0 77.91636 2 AAGGGGC 5005 0.0 77.02354 9 GGTTCGG 1800 0.0 74.90672 1 TTGGGAG 3005 0.0 73.91021 3 CCGGAGA 1195 0.0 73.862785 4 GGAGAGC 5095 0.0 73.07344 6 >>END_MODULE