##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_50220371-2.34200000035902.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3477869 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.233607131263426 33.0 33.0 33.0 33.0 33.0 2 32.25964290201845 33.0 33.0 33.0 33.0 33.0 3 32.250100276922446 33.0 33.0 33.0 33.0 33.0 4 36.066397267982204 37.0 37.0 37.0 33.0 37.0 5 36.08885239783327 37.0 37.0 37.0 33.0 37.0 6 36.044314780113915 37.0 37.0 37.0 33.0 37.0 7 36.01474322350842 37.0 37.0 37.0 33.0 37.0 8 35.915880960438706 37.0 37.0 37.0 33.0 37.0 9 35.889161725182866 37.0 37.0 37.0 33.0 37.0 10-11 35.81921616369104 37.0 37.0 37.0 33.0 37.0 12-13 35.815035873979156 37.0 37.0 37.0 33.0 37.0 14-15 37.61122040536892 40.0 37.0 40.0 33.0 40.0 16-17 37.430119708361644 40.0 37.0 40.0 33.0 40.0 18-19 37.13140259164448 40.0 37.0 40.0 33.0 40.0 20-21 37.059923763661025 40.0 37.0 40.0 33.0 40.0 22-23 36.903430951539576 40.0 37.0 40.0 33.0 40.0 24-25 36.65131104708084 38.5 37.0 40.0 33.0 40.0 26-27 36.58031685494767 37.0 37.0 40.0 33.0 40.0 28-29 36.53981087269244 37.0 37.0 40.0 33.0 40.0 30-31 36.505131878170225 37.0 37.0 40.0 33.0 40.0 32-33 36.38390189509725 37.0 37.0 40.0 33.0 40.0 34-35 36.28035227893862 37.0 37.0 40.0 33.0 40.0 36-37 36.1877432703762 37.0 37.0 40.0 33.0 40.0 38-39 36.092501327680836 37.0 37.0 40.0 33.0 40.0 40-41 35.97771221400231 37.0 37.0 40.0 33.0 40.0 42-43 35.85061930164707 37.0 37.0 40.0 27.0 40.0 44-45 35.7252098914594 37.0 37.0 40.0 27.0 40.0 46-47 35.57213799599697 37.0 37.0 40.0 27.0 40.0 48-49 35.41562750063329 37.0 37.0 40.0 27.0 40.0 50-51 34.14419016357431 37.0 35.0 38.5 24.5 38.5 52-53 34.60388473516397 37.0 35.0 38.5 27.0 40.0 54-55 35.311467021903354 37.0 37.0 40.0 27.0 40.0 56-57 35.31116367522756 37.0 37.0 40.0 27.0 40.0 58-59 35.122133266089094 37.0 37.0 40.0 27.0 40.0 60-61 34.8541378355539 37.0 33.0 40.0 27.0 40.0 62-63 34.56531600241412 37.0 33.0 40.0 27.0 40.0 64-65 34.306972027986106 37.0 33.0 37.0 27.0 40.0 66-67 33.98755933590368 37.0 33.0 37.0 24.5 40.0 68-69 33.73010254267771 37.0 33.0 37.0 22.0 40.0 70-71 33.434321275470694 37.0 33.0 37.0 22.0 40.0 72-73 33.15065374802789 37.0 33.0 37.0 22.0 38.5 74-75 32.85094723234256 37.0 33.0 37.0 22.0 37.0 76-77 32.580342732863144 37.0 33.0 37.0 22.0 37.0 78-79 32.3277289915175 37.0 33.0 37.0 22.0 37.0 80-81 32.06030244382408 37.0 33.0 37.0 18.5 37.0 82-83 31.818810743015334 37.0 33.0 37.0 15.0 37.0 84-85 31.520210795748774 35.0 33.0 37.0 15.0 37.0 86-87 31.341244739235435 33.0 33.0 37.0 15.0 37.0 88-89 31.171684298632293 33.0 33.0 37.0 15.0 37.0 90-91 30.97606192182627 33.0 33.0 37.0 15.0 37.0 92-93 30.764383017301686 33.0 30.0 37.0 15.0 37.0 94-95 30.560007579353908 33.0 27.0 37.0 15.0 37.0 96-97 30.357866411874625 33.0 27.0 37.0 15.0 37.0 98-99 30.14396114402239 33.0 27.0 37.0 15.0 37.0 100-101 28.883087747123312 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.18853674933949804 2306 2 0.18299524572292114 2306 3 0.1752770401045609 2306 4 0.3337472376159667 2306 5 0.23840384776710977 2306 6 0.1941411985273902 2306 7 0.16089859299788145 2306 8 0.1315181235489078 2306 9 0.1734367886270789 2306 10-11 0.1271838433622392 2306 12-13 0.10495167730586275 2306 14-15 0.1209506411269814 2306 16-17 0.2580124927401428 2306 18-19 0.3124221773243434 2306 20-21 0.37928240524950496 2306 22-23 0.39817762407746926 2306 24-25 0.3418087071098199 2306 26-27 0.3292370114377121 2306 28-29 0.14857549021305516 2306 30-31 0.13868252251101865 2306 32-33 0.20225535238793668 2306 34-35 0.16068583882193366 2306 36-37 0.20134077264276584 2306 38-39 0.3086512858410586 2306 40-41 0.1581232733306237 2306 42-43 0.22865539578922522 2306 44-45 0.33610487092293084 2306 46-47 0.3072548273199729 2306 48-49 0.36998239556869805 2306 50-51 0.2281024114788508 2306 52-53 0.17240370511598258 2306 54-55 0.2052406877189199 2306 56-57 0.2616417435789202 2306 58-59 0.22348524146129023 2306 60-61 0.14805204342533074 2306 62-63 0.2933544116578375 2306 64-65 0.14887859064563713 2306 66-67 0.15416311650614034 2306 68-69 0.028586320224540884 2306 70-71 0.16894595191653394 2306 72-73 0.2929907453328582 2306 74-75 0.31154795506711963 2306 76-77 0.29362946406506296 2306 78-79 0.40249422033897986 2306 80-81 0.39172367746813563 2306 82-83 0.4772453236182379 2306 84-85 0.5585314167732456 2306 86-87 0.5908794998488709 2306 88-89 0.5170050400118171 2306 90-91 0.5699602241910604 2306 92-93 0.4228835987171351 2306 94-95 0.5429187749273865 2306 96-97 0.5511220375955617 2306 98-99 0.6311593506230544 2306 100-101 0.42712009575780385 2307 1 0.13196656890962544 2307 2 0.12855760126535642 2307 3 0.11250674474401023 2307 4 0.17602287922990456 2307 5 0.17517061755135188 2307 6 0.14694955232187112 2307 7 0.17936674632726834 2307 8 0.05464146365477518 2307 9 0.1664322295217886 2307 10-11 0.0996133636042984 2307 12-13 0.16954794445898358 2307 14-15 0.10351372239878032 2307 16-17 0.27932726302702804 2307 18-19 0.347226124501411 2307 20-21 0.3631328089894126 2307 22-23 0.32463787203970895 2307 24-25 0.2976600236591338 2307 26-27 0.3272199003206424 2307 28-29 0.22843916534043984 2307 30-31 0.28152122210000385 2307 32-33 0.21863721028334737 2307 34-35 0.26385059774283093 2307 36-37 0.40953844353155233 2307 38-39 0.2613570103491085 2307 40-41 0.21580102054807782 2307 42-43 0.09704838177022879 2307 44-45 0.2513929106488533 2307 46-47 0.2855219507913844 2307 48-49 0.201569413202229 2307 50-51 0.11034435044698654 2307 52-53 0.17455741996081287 2307 54-55 0.2040629659161155 2307 56-57 0.11193217651743481 2307 58-59 0.19389535620842224 2307 60-61 0.2320589237350461 2307 62-63 0.2912729697154788 2307 64-65 0.1898410892160669 2307 66-67 0.173616528634291 2307 68-69 0.1862130793063983 2307 70-71 0.23904484313195695 2307 72-73 0.19057024729048777 2307 74-75 0.30236206033741553 2307 76-77 0.25669509880292907 2307 78-79 0.30076362406899904 2307 80-81 0.3035398822706554 2307 82-83 0.39082802287965634 2307 84-85 0.48672674386681436 2307 86-87 0.43941958721878294 2307 88-89 0.41308366587009004 2307 90-91 0.5553938368972524 2307 92-93 0.7395888403468938 2307 94-95 0.6295924282687722 2307 96-97 0.5936135625167402 2307 98-99 0.5851959907784767 2307 100-101 0.575479774431944 2201 1 0.19355470236149586 2201 2 0.1783248091639109 2201 3 0.20571483046764882 2201 4 0.23011721267242535 2201 5 0.22768453828505386 2201 6 0.26716737086101006 2201 7 0.3693215916784425 2201 8 0.3185259332631176 2201 9 0.37018497857424393 2201 10-11 0.30129437993483776 2201 12-13 0.3691829638778259 2201 14-15 0.4171794376203479 2201 16-17 0.5194540703827926 2201 18-19 0.6577497312582281 2201 20-21 0.5850643985266331 2201 22-23 0.6925032035637386 2201 24-25 0.6139841310944476 2201 26-27 0.5678859898812618 2201 28-29 0.5143493941551966 2201 30-31 0.3732875175471051 2201 32-33 0.43109802166325295 2201 34-35 0.5331243799710776 2201 36-37 0.49301722318622865 2201 38-39 0.45918525891023165 2201 40-41 0.5673913766381062 2201 42-43 0.584275816143915 2201 44-45 0.41112806576558825 2201 46-47 0.4350622156362647 2201 48-49 0.44213840856978237 2201 50-51 0.5510209682935994 2201 52-53 0.6011951533378195 2201 54-55 0.5759021404984921 2201 56-57 0.6428081299122255 2201 58-59 0.6657766439347839 2201 60-61 0.566761324184256 2201 62-63 0.5916525729160824 2201 64-65 0.6434984987897892 2201 66-67 0.6604509069974966 2201 68-69 0.5764159242832321 2201 70-71 0.5023525966296916 2201 72-73 0.5653011910928427 2201 74-75 0.6899837205311385 2201 76-77 0.6998060903335102 2201 78-79 0.7441354831303428 2201 80-81 0.766801428290961 2201 82-83 0.7561496035263282 2201 84-85 0.7545581509137484 2201 86-87 0.8152589255745006 2201 88-89 0.9213179553277655 2201 90-91 0.9776535580146586 2201 92-93 1.0426835715246554 2201 94-95 1.1060593694507475 2201 96-97 1.0389156073438421 2201 98-99 1.1257140994303896 2201 100-101 0.8757318683910498 1101 1 0.18354600670264176 1101 2 0.10305177239534657 1101 3 0.14252037908222803 1101 4 0.28596686926693593 1101 5 0.2184286975470826 1101 6 0.2426471505709884 1101 7 0.22695220531824845 1101 8 0.31624445686172464 1101 9 0.32583973247018605 1101 10-11 0.28703457579091207 1101 12-13 0.2980084194772985 1101 14-15 0.3453515270926033 1101 16-17 0.3353394476060103 1101 18-19 0.5702662518567934 1101 20-21 0.5335569587792506 1101 22-23 0.5750206237132147 1101 24-25 0.580521682732126 1101 26-27 0.43626239273859824 1101 28-29 0.4867093467634689 1101 30-31 0.5211765329540299 1101 32-33 0.4564961113861514 1101 34-35 0.46084615579222543 1101 36-37 0.5351703329029931 1101 38-39 0.4044303634695794 1101 40-41 0.4034662131235862 1101 42-43 0.4412780511819605 1101 44-45 0.5689536589886686 1101 46-47 0.521317769594333 1101 48-49 0.5163772693231437 1101 50-51 0.42084418731733564 1101 52-53 0.3921566656289954 1101 54-55 0.7131457012131861 1101 56-57 0.708870226603949 1101 58-59 0.7908097620166004 1101 60-61 0.7336405514008177 1101 62-63 0.6288690702185775 1101 64-65 0.6149698949927611 1101 66-67 0.6804337770877353 1101 68-69 0.4971431150933583 1101 70-71 0.5946360643446624 1101 72-73 0.4171340068640461 1101 74-75 0.48286081496629407 1101 76-77 0.4540031104365454 1101 78-79 0.6451220675201412 1101 80-81 0.6521884927487847 1101 82-83 0.7442129472877888 1101 84-85 0.7833127957103052 1101 86-87 0.7936558643335339 1101 88-89 0.6644774088006109 1101 90-91 0.7362712173524173 1101 92-93 0.7663707461582412 1101 94-95 0.9091047721689307 1101 96-97 0.7243486488485082 1101 98-99 0.8447386786981248 1101 100-101 0.9860045092034433 2304 1 0.1750281584828386 2304 2 0.20357889779828042 2304 3 0.21872107305136268 2304 4 0.24437197199760163 2304 5 0.2589490484228065 2304 6 0.2090907057420921 2304 7 0.18988084463206434 2304 8 0.1728776847979674 2304 9 0.30083038950134267 2304 10-11 0.2781400694565903 2304 12-13 0.17490189283618918 2304 14-15 0.3264117545991425 2304 16-17 0.430272829088878 2304 18-19 0.564740393178468 2304 20-21 0.5852939811505848 2304 22-23 0.4848005775512476 2304 24-25 0.6049530213591083 2304 26-27 0.5940377723710668 2304 28-29 0.5270538258737147 2304 30-31 0.5677285225514908 2304 32-33 0.6029161096785245 2304 34-35 0.6108264900675522 2304 36-37 0.6320978608584085 2304 38-39 0.6240359012256675 2304 40-41 0.6697568515387928 2304 42-43 0.699977719873992 2304 44-45 0.4782435793599831 2304 46-47 0.4864526092801853 2304 48-49 0.5961728717591726 2304 50-51 0.5390904105883934 2304 52-53 0.4756882658591053 2304 54-55 0.685562667764664 2304 56-57 0.7129288542583012 2304 58-59 0.5998477371608004 2304 60-61 0.6577276348688699 2304 62-63 0.583377280133746 2304 64-65 0.5578590208967853 2304 66-67 0.6475272109752055 2304 68-69 0.6664690636321495 2304 70-71 0.6290951029496625 2304 72-73 0.6805800436956346 2304 74-75 0.773242246043182 2304 76-77 0.6509514238732095 2304 78-79 0.7389866413617838 2304 80-81 0.7956953043679391 2304 82-83 0.6240751052546507 2304 84-85 0.5856214538080344 2304 86-87 0.8079741915292011 2304 88-89 0.768869933865659 2304 90-91 0.6564597283701232 2304 92-93 0.9057646219296522 2304 94-95 0.9729968920220742 2304 96-97 0.8979750924596637 2304 98-99 1.0311208992033833 2304 100-101 1.0324000828057436 2202 1 0.12193125538593819 2202 2 0.058357452576835556 2202 3 0.06029616462645038 2202 4 0.17479155241718303 2202 5 0.05035763983139674 2202 6 0.06751561416700014 2202 7 0.16002736477858548 2202 8 0.09473915665944332 2202 9 0.07445676312772065 2202 10-11 0.11013307644959269 2202 12-13 0.12522656816086908 2202 14-15 0.16225091543830672 2202 16-17 0.19103706485311278 2202 18-19 0.4073561107941899 2202 20-21 0.41243350829011405 2202 22-23 0.3505015432522143 2202 24-25 0.33319977460586614 2202 26-27 0.36679834513164167 2202 28-29 0.3253971492170322 2202 30-31 0.2546582594812321 2202 32-33 0.2742031559655729 2202 34-35 0.4185104727515352 2202 36-37 0.3573476111081604 2202 38-39 0.29921072640049573 2202 40-41 0.3120312438131876 2202 42-43 0.38239530229157026 2202 44-45 0.436526962643363 2202 46-47 0.4179877408153203 2202 48-49 0.48256980815610717 2202 50-51 0.34883899533698326 2202 52-53 0.3880259281967682 2202 54-55 0.5986079558099462 2202 56-57 0.5806860970155441 2202 58-59 0.5155736778867421 2202 60-61 0.623928101069346 2202 62-63 0.4945327912892523 2202 64-65 0.5606478986856658 2202 66-67 0.7327919462399493 2202 68-69 0.6663108884739728 2202 70-71 0.7082825821371372 2202 72-73 0.4965973597129576 2202 74-75 0.5364706842716203 2202 76-77 0.5914359393577229 2202 78-79 0.6555367579119036 2202 80-81 0.6301774888501228 2202 82-83 0.6890184951980309 2202 84-85 0.7936384368250167 2202 86-87 0.7525213110763254 2202 88-89 0.623781688777413 2202 90-91 0.6722522691626622 2202 92-93 0.7868918280568558 2202 94-95 0.7664950605202421 2202 96-97 0.7920726615572349 2202 98-99 0.8736949917774801 2202 100-101 0.8718772722829335 1102 1 -0.04168294322659705 1102 2 -0.07290590736226221 1102 3 -0.06528401357569891 1102 4 -0.006645414200662003 1102 5 -0.05306227248330231 1102 6 -0.050681174109442395 1102 7 -0.09311542671468942 1102 8 0.011893840674396472 1102 9 0.11588493336438432 1102 10-11 0.06572463888649338 1102 12-13 0.10514209737111457 1102 14-15 0.13394194428996542 1102 16-17 0.0968480726305998 1102 18-19 0.23216823030427491 1102 20-21 0.2862990884451051 1102 22-23 0.23770478143454454 1102 24-25 0.3053930559345517 1102 26-27 0.3336225265923929 1102 28-29 0.2926978786900136 1102 30-31 0.4080044817952597 1102 32-33 0.3062613815103603 1102 34-35 0.25413053639184113 1102 36-37 0.3318020730086886 1102 38-39 0.24007664773439075 1102 40-41 0.25459064259561615 1102 42-43 0.2658554365814396 1102 44-45 0.28654071424191585 1102 46-47 0.2551495501311223 1102 48-49 0.29482091582706715 1102 50-51 0.3168057199499046 1102 52-53 0.3238254589017089 1102 54-55 0.4651041297322749 1102 56-57 0.5148751820758548 1102 58-59 0.5145475301214688 1102 60-61 0.4872365151908937 1102 62-63 0.5691110175767378 1102 64-65 0.5259620153688473 1102 66-67 0.5899831619276767 1102 68-69 0.6611063435996627 1102 70-71 0.5800238014223353 1102 72-73 0.5274604727035168 1102 74-75 0.48747373863511 1102 76-77 0.6144872740170939 1102 78-79 0.5554622555805153 1102 80-81 0.617193658873262 1102 82-83 0.5965236767075375 1102 84-85 0.76003562244723 1102 86-87 0.7309108190127951 1102 88-89 0.6625702483757969 1102 90-91 0.6732093688170764 1102 92-93 0.7544516910282688 1102 94-95 0.7106990228373817 1102 96-97 0.8914257561891219 1102 98-99 0.8941340449247939 1102 100-101 0.6931344585085917 2305 1 0.09432902222815187 2305 2 0.07021390887774004 2305 3 0.145655455541295 2305 4 0.18925959466300668 2305 5 0.14280922117186634 2305 6 0.17699646253673507 2305 7 0.20882445246456172 2305 8 0.24040809677282482 2305 9 0.05188632944617666 2305 10-11 0.1554430134262006 2305 12-13 0.2342131577030102 2305 14-15 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1213 82-83 -0.3418483209592438 1213 84-85 -0.3563037335595389 1213 86-87 -0.3671075838201112 1213 88-89 -0.6172092522517474 1213 90-91 -0.646623270547007 1213 92-93 -0.7312135855434931 1213 94-95 -0.6750589091103549 1213 96-97 -0.640127810433988 1213 98-99 -0.8688849106438177 1213 100-101 -0.6650647938680017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1729.0 3 256.0 4 296.0 5 354.0 6 863.0 7 1822.0 8 4248.0 9 4114.0 10 4822.0 11 5613.0 12 6920.0 13 9082.0 14 12544.0 15 16456.0 16 18327.0 17 18055.0 18 18092.0 19 18928.0 20 20499.0 21 22460.0 22 24922.0 23 27204.0 24 29694.0 25 33626.0 26 37717.0 27 42953.0 28 49565.0 29 58608.0 30 70072.0 31 86310.0 32 110932.0 33 147956.0 34 214024.0 35 348290.0 36 679927.0 37 1000279.0 38 330134.0 39 176.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.503756514416075 32.752220020707696 19.575809489200562 15.168213975675668 2 29.970485807756386 26.540455722656553 30.600819684893608 12.888238784693447 3 33.566633729235875 24.514413104562315 25.01510432940813 16.903848836793685 4 29.026603509570837 24.49327094480575 29.421570732536807 17.058554813086605 5 28.802813146270633 22.893390115076706 30.631732924043607 17.67206381460905 6 26.42710960897807 23.533480524957852 32.16225341465748 17.8771564514066 7 30.446870581188062 23.496401593148196 26.237641725968142 19.819086099695596 8 26.867407286240557 28.750884112696433 25.342183291825155 19.039525309237852 9 24.858780811753718 26.690030683987747 29.196000054129584 19.255188450128948 10-11 19.91517610709718 31.78958550293026 33.1448821742438 15.150356215728767 12-13 20.13263621385574 31.078161613241505 33.12162139593971 15.667580776963039 14-15 20.168922849044062 31.08292434868643 33.26378386721139 15.484368935058116 16-17 19.84353546898401 31.156945231655524 33.227053238855525 15.772466060504936 18-19 19.184125817009928 31.53713744307237 32.826748551672395 16.451988188245306 20-21 19.203262509727846 31.80327972689768 32.3359035285697 16.657554234804767 22-23 19.271531875934368 31.256352132718213 32.39424248018365 17.07787351116377 24-25 19.18666643916374 30.98265936073799 32.55785201763764 17.272822182460622 26-27 19.178183841676866 31.43404588311694 32.369093670045636 17.018676605160564 28-29 18.977319706431008 31.673876248712574 32.177279373104874 17.17152467175155 30-31 18.423863641607028 32.723410182583294 32.00971112895263 16.843015046857047 32-33 18.175004932918085 33.116236858533135 32.073963192930755 16.634795015618025 34-35 18.54471037850622 32.61183400644677 32.49197759756923 16.351478017477774 36-37 18.56127184354831 32.15574262078443 32.95641430169158 16.326571233975677 38-39 18.883393549349364 32.01948607299979 32.29693124065188 16.800189136998974 40-41 18.74403244761717 32.01873957504031 31.746736752304184 17.490491225038337 42-43 19.032808281756537 31.59973933766549 31.703395463050015 17.664056917527954 44-45 18.70248518716289 31.53200752707621 31.92797812382173 17.837529161939166 46-47 18.817782145547728 31.787288962317696 31.81726768867482 17.57766120345976 48-49 18.801728093962332 32.34265736787809 31.258941582120315 17.59667295603926 50-51 19.321290155787256 32.25761681736027 30.4431332651615 17.977959761690972 52-53 19.409910942391605 32.056108644541155 30.544210808283733 17.98976960478351 54-55 19.79299516023273 32.11223229036642 30.435925225783205 17.658847323617643 56-57 19.90586239551786 32.181328124943654 30.30045128638618 17.612358193152307 58-59 19.763520238167356 32.70485537160282 30.186731747281815 17.34489264294801 60-61 19.610706382060698 32.58411751489601 30.02121594841721 17.783960154626076 62-63 19.380677906226456 32.48094357510283 30.38019126717205 17.75818725149867 64-65 19.310188588695294 32.101392993530084 30.745013167378126 17.843405250396493 66-67 19.76929298616756 31.686064120633052 30.47955479122113 18.06508810197826 68-69 20.15117567379903 31.72642844442291 30.53169761577308 17.590698266004985 70-71 19.8650637374438 32.001469494366766 30.61045293213292 17.523013836056506 72-73 19.94226028603996 31.957593721065624 30.26170588789949 17.83844010499493 74-75 20.33866588856186 31.57778722216584 29.860656191469143 18.222890697803162 76-77 20.446544162805385 31.364616702083797 29.462817781032758 18.726021354078064 78-79 20.330543813607292 31.50378576303952 29.691530312201092 18.47414011115209 80-81 20.139723106005714 31.88393321264372 29.289019912345022 18.687323769005552 82-83 19.86219922513926 31.974896854217693 29.211698975126026 18.95120494551702 84-85 19.975830066330317 31.8587414473262 29.71214560798704 18.453282878356443 86-87 20.355408762909494 31.643929574141726 29.99610328402877 18.004558378920006 88-89 20.813315805452056 31.56300770943138 29.782216315496097 17.841460169620472 90-91 20.884057130809065 31.507348731389495 29.298604783705855 18.30998935409559 92-93 20.643767869483863 31.606476782665982 29.229944471495845 18.51981087635431 94-95 20.56678207586902 31.393211066768306 29.300432218588963 18.739574638773714 96-97 20.690063428117877 31.85499268580816 29.00890393258945 18.446039953484508 98-99 21.328957949472912 31.59065028503259 28.651615876909304 18.428775888585196 100-101 21.785250670585985 31.436309438565324 28.143281210857847 18.635158679990845 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 167975.0 1 92908.0 2 13508.5 3 9356.5 4 8598.0 5 7333.0 6 7527.0 7 8492.0 8 9048.0 9 8859.0 10 8108.0 11 7460.0 12 7019.5 13 6669.5 14 6531.0 15 6407.5 16 6336.0 17 6336.0 18 6347.0 19 6423.5 20 6565.5 21 6785.5 22 7232.0 23 8083.0 24 9277.5 25 10967.5 26 13296.0 27 16363.0 28 19965.5 29 23685.5 30 28544.5 31 34583.0 32 43472.0 33 58503.5 34 78713.5 35 100456.5 36 129572.0 37 167716.0 38 196409.0 39 201007.5 40 199507.0 41 198696.5 42 191297.0 43 185921.5 44 174591.5 45 160682.0 46 156855.0 47 145481.5 48 119992.0 49 96397.5 50 77441.0 51 62102.0 52 50376.5 53 39560.0 54 31417.0 55 26398.0 56 21261.0 57 15777.0 58 11401.5 59 8210.0 60 5944.0 61 4558.5 62 3574.5 63 2721.5 64 2205.0 65 1678.5 66 1153.5 67 856.5 68 656.0 69 532.5 70 423.0 71 328.0 72 259.0 73 206.0 74 167.5 75 144.5 76 123.0 77 97.5 78 78.0 79 66.0 80 53.5 81 38.5 82 31.5 83 29.5 84 25.5 85 20.0 86 16.0 87 12.5 88 8.0 89 8.5 90 9.0 91 6.5 92 5.0 93 4.5 94 3.0 95 2.0 96 1.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30360545494956825 2 0.27910769497068466 3 0.2491468195035523 4 0.2361791085288146 5 0.24279235359353674 6 0.19141031476458714 7 0.15811406352568197 8 0.15702144042803223 9 0.13577279650268598 10-11 0.12080673538882575 12-13 0.14120715875152284 14-15 0.14494507987506142 16-17 0.1384037179088689 18-19 0.12593918862383835 20-21 0.1320348753791474 22-23 0.16130567310039567 24-25 0.5761574113343545 26-27 0.22213315107613313 28-29 0.19534950856400862 30-31 0.2961871191813148 32-33 0.32666555295786015 34-35 0.33463020027493845 36-37 0.32611924140903525 38-39 0.33349444731817096 40-41 0.33723236844170956 42-43 0.3474110151934992 44-45 0.32922459126551346 46-47 0.34728162561614595 48-49 0.34416189913996187 50-51 0.34840300195320756 52-53 0.3343857977399379 54-55 0.327211864506685 56-57 0.3354496675981758 58-59 0.32775817605550983 60-61 0.3066820515666346 62-63 0.2934699380568963 64-65 0.3044392988925115 66-67 0.31890217831666456 68-69 0.3060638569192802 70-71 0.2878055498927648 72-73 0.28442704426187415 74-75 0.2764048904659721 76-77 0.27830260426715325 78-79 0.28776242003364705 80-81 0.2960577296039615 82-83 0.31779517859930895 84-85 0.3091404535363465 86-87 0.3118863879001768 88-89 0.3166162957834237 90-91 0.3107650115631152 92-93 0.29300988622630697 94-95 0.2893582248210039 96-97 0.30584820762369136 98-99 0.31332404987076856 100-101 0.31451730930635974 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3477869.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.51689114454838 #Duplication Level Percentage of deduplicated Percentage of total 1 83.13077347010169 37.00723593330627 2 7.9553062093802165 7.082910010890579 3 3.012616313241625 4.023369374306043 4 1.4539149557405149 2.588950952725255 5 0.913317256915591 2.0329022453274446 6 0.6249683029973808 1.6692987547996518 7 0.4014069998579707 1.250857420213592 8 0.33651559989770097 1.198450266327068 9 0.23204656184645767 0.9296992380766924 >10 1.6523304530311735 13.92941806746666 >50 0.15435944647836353 4.769156588259985 >100 0.11949535161055666 10.829311674597907 >500 0.010853889333221971 3.177321213246405 >1k 0.0019737816413611642 1.1460425585364906 >5k 0.0 0.0 >10k+ 1.2140792631392751E-4 8.365075701919862 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 171414 4.928707780540325 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 138134 3.9717999729144484 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.587791547065171E-4 0.0 0.0 2 2.587791547065171E-4 0.0 0.0 3 2.587791547065171E-4 0.0 0.0 4 2.875323941183524E-4 0.0 0.0 5 3.7379211235385806E-4 0.0 0.0 6 5.175583094130343E-4 0.0 0.0 7 6.038180276485399E-4 0.0 0.0 8 8.338439429432218E-4 0.0 0.0 9 0.001236389294708915 0.0 0.0 10-11 0.0028321940820657705 0.0 0.0 12-13 0.008899127597963005 0.0 0.0 14-15 0.021176760826816653 0.0 0.0 16-17 0.044754417144521545 0.0 0.0 18-19 0.06787202163163708 0.0 0.0 20-21 0.07655549993401131 0.0 0.0 22-23 0.08368630330814646 0.0 0.0 24-25 0.09165095062522481 0.0 0.0 26-27 0.10177209089819081 0.0 0.0 28-29 0.11338839962057226 0.0 0.0 30-31 0.12602544834207385 0.0 0.0 32-33 0.14010015903416717 0.0 0.0 34-35 0.15522436296479253 0.0 0.0 36-37 0.17203063140101021 0.0 0.0 38-39 0.18952697758311193 0.0 0.0 40-41 0.209582362072867 0.0 0.0 42-43 0.22912018825320907 0.0 0.0 44-45 0.2510876631638512 0.0 0.0 46-47 0.2736877093415537 0.0 0.0 48-49 0.29807045636278995 0.0 0.0 50-51 0.32463844957932575 0.0 0.0 52-53 0.35395237715969174 0.0 0.0 54-55 0.38453144727417854 0.0 0.0 56-57 0.41775581541455414 0.0 0.0 58-59 0.4540424035522902 0.0 0.0 60-61 0.4890926023953174 0.0 0.0 62-63 0.5273344108130582 0.0 0.0 64-65 0.5691559975375726 0.0 0.0 66-67 0.6140829341185652 0.0 0.0 68-69 0.6631215839354502 0.0 0.0 70-71 0.7178246219164667 0.0 0.0 72-73 0.7780626584842615 0.0 0.0 74-75 0.8443388753285417 0.0 0.0 76-77 0.9188816485037246 0.0 0.0 78-79 1.0038330943459917 0.0 0.0 80-81 1.100990290318583 0.0 0.0 82-83 1.209390002901202 0.0 0.0 84-85 1.3328564129356222 0.0 0.0 86-87 1.475170571404501 2.8753239411835237E-5 0.0 88-89 1.6382445687287244 2.8753239411835237E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACGG 40 7.5636526E-6 59.35515 1 ACGCGGA 25 0.0050287796 56.98094 1 GTCGCAC 35 2.7933397E-4 54.267563 1 CGGGTCG 75 1.3674617E-7 44.31851 1 CGCGGAG 165 0.0 43.167377 1 ACGCGGT 50 0.0016240904 37.96266 3 CGCGATA 90 5.7464604E-7 36.908142 3 AATGCGC 70 2.0582232E-4 33.91723 1 CGCAGCC 200 0.0 33.238884 1 ACTGGCG 255 0.0 31.649687 2 CGCGTTG 75 3.1090667E-4 31.615503 6 GTTTGAT 14590 0.0 31.386196 3 GTGTTTG 51385 0.0 29.598448 1 ACGGTGG 245 0.0 29.071909 1 TGTTTGA 52325 0.0 28.942907 2 GTTTGAG 25665 0.0 28.21499 3 CCGTGGC 140 5.828788E-7 27.133781 1 CGGTCAG 70 0.008407569 27.133781 1 CCCTCGA 105 6.956185E-5 27.116186 3 TTGAGTG 3770 0.0 27.06068 5 >>END_MODULE