FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_50220371-2.34200000035902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_50220371-2.34200000035902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477869
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1714144.928707780540325No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1381343.9717999729144484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGG407.5636526E-659.355151
ACGCGGA250.005028779656.980941
GTCGCAC352.7933397E-454.2675631
CGGGTCG751.3674617E-744.318511
CGCGGAG1650.043.1673771
ACGCGGT500.001624090437.962663
CGCGATA905.7464604E-736.9081423
AATGCGC702.0582232E-433.917231
CGCAGCC2000.033.2388841
ACTGGCG2550.031.6496872
CGCGTTG753.1090667E-431.6155036
GTTTGAT145900.031.3861963
GTGTTTG513850.029.5984481
ACGGTGG2450.029.0719091
TGTTTGA523250.028.9429072
GTTTGAG256650.028.214993
CCGTGGC1405.828788E-727.1337811
CGGTCAG700.00840756927.1337811
CCCTCGA1056.956185E-527.1161863
TTGAGTG37700.027.060685
Produced by FastQC (version 0.11.2)