FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_50110001-2.342000000358f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_50110001-2.342000000358f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6109255
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC212020.3470472258892451No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129880.21259548013628504No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121650.19912411578825898No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG94110.15404496947663832No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA83070.13597402629289496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGG3250.059.9016081
TGTACCG1850.056.4560432
CGGACGG1350.056.2761271
CGAGTGG2950.051.5069581
CGCGGAG4400.047.482981
CGGAAAG4200.047.459215
CGAGGGG11250.042.629161
GTTACGG2800.042.3955151
ATGACCG1155.4569682E-1141.2895471
ATTACGG2550.040.965711
GAGCGGC6050.039.9649549
GGACGGG9600.039.569151
CACGGGG6300.039.1923031
CGGCAGC3150.037.6849061
GATCCGG2400.037.590691
CGAGCGG1650.037.410831
TGCCGGC1551.8189894E-1236.7543752
AACGCGG1302.0736479E-1036.5253681
CTGACCG2100.036.177511
AGCGCGG1750.035.2730671
Produced by FastQC (version 0.11.2)