##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_44065-1.342000000357db.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3955270 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.255885186093494 33.0 33.0 33.0 33.0 33.0 2 32.25999944378007 33.0 33.0 33.0 33.0 33.0 3 32.23908886119026 33.0 33.0 33.0 33.0 33.0 4 36.04148718039477 37.0 37.0 37.0 37.0 37.0 5 36.05895096921323 37.0 37.0 37.0 37.0 37.0 6 36.04748373688775 37.0 37.0 37.0 33.0 37.0 7 36.020718939541425 37.0 37.0 37.0 33.0 37.0 8 35.95533023030033 37.0 37.0 37.0 33.0 37.0 9 35.9211439421329 37.0 37.0 37.0 33.0 37.0 10-11 35.88947593969564 37.0 37.0 37.0 33.0 37.0 12-13 35.88875993295022 37.0 37.0 37.0 33.0 37.0 14-15 37.74388979766236 40.0 37.0 40.0 33.0 40.0 16-17 37.72959519830505 40.0 37.0 40.0 33.0 40.0 18-19 37.68058324716138 40.0 37.0 40.0 33.0 40.0 20-21 37.66942395841498 40.0 37.0 40.0 33.0 40.0 22-23 37.63293441408551 40.0 37.0 40.0 33.0 40.0 24-25 37.41418234406248 40.0 37.0 40.0 33.0 40.0 26-27 37.37511105436544 40.0 37.0 40.0 33.0 40.0 28-29 37.36622265989427 40.0 37.0 40.0 33.0 40.0 30-31 37.3598577341117 40.0 37.0 40.0 33.0 40.0 32-33 37.2765107059695 40.0 37.0 40.0 33.0 40.0 34-35 37.2049970039972 40.0 37.0 40.0 33.0 40.0 36-37 37.13474844953694 40.0 37.0 40.0 33.0 40.0 38-39 37.08755508473505 40.0 37.0 40.0 33.0 40.0 40-41 36.994218093834306 40.0 37.0 40.0 33.0 40.0 42-43 36.897194249697236 40.0 37.0 40.0 33.0 40.0 44-45 36.792562833889974 40.0 37.0 40.0 33.0 40.0 46-47 36.68816781155269 40.0 37.0 40.0 33.0 40.0 48-49 36.55817820275227 38.5 37.0 40.0 33.0 40.0 50-51 35.38514488265024 37.0 35.0 38.5 30.0 38.5 52-53 35.80073092355263 37.0 37.0 38.5 33.0 40.0 54-55 36.55454469606373 37.0 37.0 40.0 33.0 40.0 56-57 36.602287328045875 37.0 37.0 40.0 33.0 40.0 58-59 36.4358635440817 37.0 37.0 40.0 33.0 40.0 60-61 36.17715920784168 37.0 37.0 40.0 33.0 40.0 62-63 35.909779231253495 37.0 37.0 40.0 33.0 40.0 64-65 35.66581661934583 37.0 37.0 40.0 33.0 40.0 66-67 35.373091596781 37.0 37.0 38.5 30.0 40.0 68-69 35.14963112505593 37.0 33.0 37.0 27.0 40.0 70-71 34.84394807434133 37.0 33.0 37.0 27.0 40.0 72-73 34.59061631696446 37.0 33.0 37.0 27.0 40.0 74-75 34.330556068233015 37.0 33.0 37.0 27.0 37.0 76-77 34.101661959866206 37.0 33.0 37.0 27.0 37.0 78-79 33.872433993128155 37.0 33.0 37.0 27.0 37.0 80-81 33.65217418785569 37.0 33.0 37.0 27.0 37.0 82-83 33.46002409443604 37.0 33.0 37.0 27.0 37.0 84-85 33.22042338449714 37.0 33.0 37.0 27.0 37.0 86-87 33.09822173960311 37.0 33.0 37.0 27.0 37.0 88-89 32.98566924634728 37.0 33.0 37.0 27.0 37.0 90-91 32.85487804878049 37.0 33.0 37.0 27.0 37.0 92-93 32.70589605766484 33.0 33.0 37.0 27.0 37.0 94-95 32.563836097156454 33.0 33.0 37.0 27.0 37.0 96-97 32.419067219178466 33.0 33.0 37.0 24.5 37.0 98-99 32.27455293823178 33.0 33.0 37.0 22.0 37.0 100-101 30.925848930667186 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.21633404294038883 2306 2 0.23008388886128728 2306 3 0.20413030129095944 2306 4 0.3068553282071065 2306 5 0.2986424256468112 2306 6 0.30852142529431603 2306 7 0.2822310787603257 2306 8 0.36552350968784 2306 9 0.3781255793237648 2306 10-11 0.28910730450517264 2306 12-13 0.31925807511517945 2306 14-15 0.532065340962319 2306 16-17 0.4915383586843305 2306 18-19 0.5264561732414847 2306 20-21 0.5167306549859845 2306 22-23 0.5125785712236066 2306 24-25 0.5285362562295717 2306 26-27 0.514133824694774 2306 28-29 0.3854374293653535 2306 30-31 0.2804353456828039 2306 32-33 0.37963069904165536 2306 34-35 0.35866943044995736 2306 36-37 0.35874053490073976 2306 38-39 0.31189206632115685 2306 40-41 0.2538610936598005 2306 42-43 0.32303645345086807 2306 44-45 0.45650090243650254 2306 46-47 0.4187264184766164 2306 48-49 0.40311729728950496 2306 50-51 0.40365568055903367 2306 52-53 0.33975876526461946 2306 54-55 0.34859784737958677 2306 56-57 0.4018360772186398 2306 58-59 0.4576646541532696 2306 60-61 0.33457698007016035 2306 62-63 0.31824619168233426 2306 64-65 0.2799790149814285 2306 66-67 0.411592546880307 2306 68-69 0.283170664735934 2306 70-71 0.2715807084443682 2306 72-73 0.2630229396646584 2306 74-75 0.40882347216737003 2306 76-77 0.41808525063098045 2306 78-79 0.37508799454624864 2306 80-81 0.35169742154972994 2306 82-83 0.2366631791074525 2306 84-85 0.3390973299393991 2306 86-87 0.32283645329915345 2306 88-89 0.3623626591765543 2306 90-91 0.215321097565905 2306 92-93 0.35034078184894923 2306 94-95 0.20876361249766262 2306 96-97 0.16138143117192527 2306 98-99 0.25308219655315867 2306 100-101 0.2306417575149915 2307 1 0.18013569583295208 2307 2 0.16565083327273555 2307 3 0.15357170053635372 2307 4 0.2613142349900741 2307 5 0.20788199324740475 2307 6 0.20792520737497 2307 7 0.26810840720625606 2307 8 0.2887203464519743 2307 9 0.2893038168040434 2307 10-11 0.2613808520017855 2307 12-13 0.2581429100239063 2307 14-15 0.4282022561285359 2307 16-17 0.43665514159651764 2307 18-19 0.4487682429774864 2307 20-21 0.38660615065577275 2307 22-23 0.38079500851042525 2307 24-25 0.29100394223655 2307 26-27 0.2420980289927499 2307 28-29 0.12185276811781875 2307 30-31 0.13091124524214592 2307 32-33 0.21522536136396297 2307 34-35 0.2053347395046572 2307 36-37 0.3254413681350812 2307 38-39 0.1941548660564223 2307 40-41 0.115001431139504 2307 42-43 0.05898013518137191 2307 44-45 0.2190399196385755 2307 46-47 0.2046852036401745 2307 48-49 0.17513159746881968 2307 50-51 0.08556024043820543 2307 52-53 0.08111830050879831 2307 54-55 0.08473023717261441 2307 56-57 0.05342997022750495 2307 58-59 0.22434732932764234 2307 60-61 0.27542707142612954 2307 62-63 0.3280349133209768 2307 64-65 0.31512306326716555 2307 66-67 0.3107761129875257 2307 68-69 0.20566041193728068 2307 70-71 0.17071969962555045 2307 72-73 0.14000044290312275 2307 74-75 0.24157789713385114 2307 76-77 0.18285567279173165 2307 78-79 0.12676605949666708 2307 80-81 0.1318624279701055 2307 82-83 0.11210544948718848 2307 84-85 0.1712689999718151 2307 86-87 0.28634276902312905 2307 88-89 0.1878318555268379 2307 90-91 0.12264694140658605 2307 92-93 0.26005059229237304 2307 94-95 0.17735701337219467 2307 96-97 0.0850175685396195 2307 98-99 0.24069381771153786 2307 100-101 0.12157856633495001 2201 1 0.13597408083114004 2201 2 0.1635054653645298 2201 3 0.18201742308317392 2201 4 0.2236285977235326 2201 5 0.21210122723843483 2201 6 0.2802647001295355 2201 7 0.2836238857857154 2201 8 0.22570865825212394 2201 9 0.2709247162135 2201 10-11 0.24173538623524138 2201 12-13 0.2513567605015865 2201 14-15 0.4269168933872578 2201 16-17 0.34843081375282026 2201 18-19 0.3879603767328703 2201 20-21 0.3751277068903889 2201 22-23 0.32188757727873707 2201 24-25 0.22933074733145276 2201 26-27 0.23316739881115467 2201 28-29 0.13887416908387706 2201 30-31 0.09836340556280732 2201 32-33 0.15789806828215802 2201 34-35 0.1743163082129584 2201 36-37 0.10090611695229512 2201 38-39 0.10794271439948488 2201 40-41 0.14729990167892737 2201 42-43 0.18686814349019443 2201 44-45 0.20973810625834233 2201 46-47 0.1718654482599291 2201 48-49 0.1363219723836835 2201 50-51 0.07317544579719026 2201 52-53 0.11790348449552113 2201 54-55 0.19505483980251626 2201 56-57 0.21288363365223262 2201 58-59 0.2041038055924318 2201 60-61 0.2346677951081091 2201 62-63 0.23594468532409962 2201 64-65 0.3194259596129996 2201 66-67 0.25683649288664157 2201 68-69 0.18503704127859777 2201 70-71 0.14088026558598443 2201 72-73 0.19855333990135904 2201 74-75 0.2576057358737529 2201 76-77 0.17029624668521848 2201 78-79 0.23464822240079286 2201 80-81 0.30290784206744803 2201 82-83 0.27573158366532624 2201 84-85 0.27218683289240886 2201 86-87 0.15920151116510795 2201 88-89 0.23494232062794396 2201 90-91 0.22723052156039358 2201 92-93 0.14201120365907371 2201 94-95 0.1979659270924543 2201 96-97 0.27864910449799396 2201 98-99 0.27211850463010023 2201 100-101 0.21789361277199149 1101 1 0.263670420413753 1101 2 0.19280254968970922 1101 3 0.17887831588032554 1101 4 0.27089447072224004 1101 5 0.20537422565868724 1101 6 0.2596579771650198 1101 7 0.33752285051858166 1101 8 0.2849878785601021 1101 9 0.3470015915628366 1101 10-11 0.3437238334979469 1101 12-13 0.3385321071005265 1101 14-15 0.4729352649213183 1101 16-17 0.4688465450457002 1101 18-19 0.43126569571155215 1101 20-21 0.49060730793214447 1101 22-23 0.5255169477030961 1101 24-25 0.5899900674991372 1101 26-27 0.4504294125669688 1101 28-29 0.4289073739520006 1101 30-31 0.4590297905960625 1101 32-33 0.3628394851673846 1101 34-35 0.4563922636879596 1101 36-37 0.3790121947071512 1101 38-39 0.42387422653543183 1101 40-41 0.39787751451371633 1101 42-43 0.4718545832737533 1101 44-45 0.5060052139264144 1101 46-47 0.4509830513577029 1101 48-49 0.4089502321342806 1101 50-51 0.3175282392306116 1101 52-53 0.35533669868734563 1101 54-55 0.5736073017159242 1101 56-57 0.5710502577855223 1101 58-59 0.5257516590507763 1101 60-61 0.540320841002341 1101 62-63 0.4651178221099812 1101 64-65 0.4910466830263971 1101 66-67 0.5988204417043335 1101 68-69 0.4173209777447866 1101 70-71 0.4782070261913347 1101 72-73 0.2869994491549335 1101 74-75 0.4348843112123717 1101 76-77 0.4280918452259215 1101 78-79 0.44865241998825667 1101 80-81 0.5673442700522031 1101 82-83 0.5468322945253661 1101 84-85 0.6143966651667299 1101 86-87 0.5768616565283153 1101 88-89 0.5268026342983703 1101 90-91 0.5144989609796511 1101 92-93 0.47257659956309084 1101 94-95 0.6320267493370721 1101 96-97 0.5310678597455833 1101 98-99 0.5060328871103863 1101 100-101 0.3907057032080381 2304 1 0.11250488241019951 2304 2 0.11934014302577367 2304 3 0.1092405864770285 2304 4 0.20211439078554605 2304 5 0.20420229899601594 2304 6 0.16139591660950003 2304 7 0.21936000471839634 2304 8 0.23049373707259946 2304 9 0.22340233309562052 2304 10-11 0.1525995864070282 2304 12-13 0.2241721947036126 2304 14-15 0.20223163818040746 2304 16-17 0.16044243209420728 2304 18-19 0.2997669162816905 2304 20-21 0.2059255191972582 2304 22-23 0.1122805426576221 2304 24-25 0.1576491058826548 2304 26-27 0.08210752277918942 2304 28-29 0.06816467551688987 2304 30-31 0.08014845601637433 2304 32-33 0.16418603517936958 2304 34-35 0.08925664702544367 2304 36-37 0.08100119724691979 2304 38-39 0.005452471918225399 2304 40-41 -0.00607964336237643 2304 42-43 0.17492920953718993 2304 44-45 0.05331200182068585 2304 46-47 -0.00677087542602095 2304 48-49 0.059197372368714696 2304 50-51 0.026033225747184474 2304 52-53 0.19217953543132182 2304 54-55 0.32731619854020266 2304 56-57 0.2289557190803393 2304 58-59 0.08309536666986617 2304 60-61 0.17303682811742505 2304 62-63 0.09356954738419176 2304 64-65 0.2320329574966422 2304 66-67 0.35439056788595025 2304 68-69 0.23675572711881188 2304 70-71 0.19179760953195313 2304 72-73 0.19081605653035894 2304 74-75 0.2532401617670672 2304 76-77 0.23603037063372767 2304 78-79 0.17875062600794678 2304 80-81 0.1953283903114169 2304 82-83 0.2494666637181311 2304 84-85 0.2569034405078483 2304 86-87 0.2665355659169606 2304 88-89 0.18003989116505892 2304 90-91 0.2109686103655548 2304 92-93 0.1006190023265745 2304 94-95 0.19893663337730771 2304 96-97 0.19245704565107502 2304 98-99 0.2657661774043305 2304 100-101 0.22873907218336242 2202 1 0.15705928701291327 2202 2 0.12783117606927874 2202 3 0.1166594052748735 2202 4 0.22848921798856026 2202 5 0.23627670491332253 2202 6 0.2834188274863152 2202 7 0.2740034934817146 2202 8 0.2904087404720528 2202 9 0.2896265573321841 2202 10-11 0.2746607026190304 2202 12-13 0.3008357186733548 2202 14-15 0.34565836917992954 2202 16-17 0.28009394192387305 2202 18-19 0.3279145942805499 2202 20-21 0.35147100305960777 2202 22-23 0.29021511294003943 2202 24-25 0.30253154231493085 2202 26-27 0.3026778092986646 2202 28-29 0.18947174987145843 2202 30-31 0.19688793876543542 2202 32-33 0.08136734414345881 2202 34-35 0.22424410704849151 2202 36-37 0.34779829610631197 2202 38-39 0.22914224459648125 2202 40-41 0.14453564739871183 2202 42-43 0.28134236531842305 2202 44-45 0.26916475106428805 2202 46-47 0.29540456922450886 2202 48-49 0.33752056896391736 2202 50-51 0.12287204236773164 2202 52-53 0.24372120286825805 2202 54-55 0.38351604119994676 2202 56-57 0.33434862808795884 2202 58-59 0.32843852657973827 2202 60-61 0.2899626132267912 2202 62-63 0.3403380127826878 2202 64-65 0.3814347153528743 2202 66-67 0.4590288188210607 2202 68-69 0.3971213891494827 2202 70-71 0.3439024975553977 2202 72-73 0.3229629704225161 2202 74-75 0.27640943810460783 2202 76-77 0.30236695142055225 2202 78-79 0.24545734665505137 2202 80-81 0.21452780367465607 2202 82-83 0.34298572427769614 2202 84-85 0.3747317753098329 2202 86-87 0.3484804639641723 2202 88-89 0.27109614544004756 2202 90-91 0.2918143861084559 2202 92-93 0.26556807214991096 2202 94-95 0.35529977303423976 2202 96-97 0.24788004649528972 2202 98-99 0.15964859945825793 2202 100-101 0.36364087830977354 1102 1 -0.005527428921084265 1102 2 -0.004925711394719201 1102 3 0.04389047267330426 1102 4 -0.029127520224406567 1102 5 -0.048003484910800864 1102 6 -0.03599942859512595 1102 7 -0.04783216737068585 1102 8 0.055048563689283014 1102 9 0.02675954289613003 1102 10-11 0.03346270953819186 1102 12-13 -0.040570686985141435 1102 14-15 0.05637571146108655 1102 16-17 -0.004225417238586715 1102 18-19 0.06285964400070299 1102 20-21 0.09855617033306174 1102 22-23 0.058064784189291174 1102 24-25 0.02386502055217221 1102 26-27 0.08124255870546193 1102 28-29 0.02470253199031447 1102 30-31 -0.07667139938595824 1102 32-33 -0.060644374960318714 1102 34-35 -0.03505634668187696 1102 36-37 -0.07464324422226554 1102 38-39 -0.02436884508092163 1102 40-41 -0.17276667196950513 1102 42-43 -0.09293741696345847 1102 44-45 0.026075535537835037 1102 46-47 0.013075601121485647 1102 48-49 -0.016106104223425177 1102 50-51 0.05502305035555821 1102 52-53 0.05223498685275274 1102 54-55 0.12316933595261759 1102 56-57 0.18936513354903184 1102 58-59 0.1429895961517289 1102 60-61 0.1356341749286898 1102 62-63 0.2406441222938156 1102 64-65 0.1249715481156457 1102 66-67 0.17403565647433794 1102 68-69 0.16095212779717372 1102 70-71 0.11275925098328088 1102 72-73 0.14359253263157967 1102 74-75 0.17633143904649273 1102 76-77 0.23211334693577612 1102 78-79 0.19076891270406549 1102 80-81 0.21788663103976091 1102 82-83 0.18051945971938466 1102 84-85 0.3116999326754737 1102 86-87 0.18034709802746107 1102 88-89 0.1278525166838662 1102 90-91 0.07112391897447168 1102 92-93 0.19453357143444094 1102 94-95 0.23398678511560433 1102 96-97 0.2040081234392659 1102 98-99 0.19088026005707093 1102 100-101 0.17892065606340068 2305 1 0.09755810933042852 2305 2 0.04886351459367688 2305 3 0.07246122764447449 2305 4 0.09327687344554647 2305 5 0.08919204777228629 2305 6 0.09679861706599269 2305 7 0.10177220474405857 2305 8 0.15714615489476103 2305 9 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1213 56-57 -0.269992814197316 1213 58-59 -0.17174990374986976 1213 60-61 -0.07876351694718409 1213 62-63 1.5518479739995428E-4 1213 64-65 0.026388605704774193 1213 66-67 0.06592644440627993 1213 68-69 0.048359322638908964 1213 70-71 0.06518011565476911 1213 72-73 0.11157233960764046 1213 74-75 0.025077222564718227 1213 76-77 0.04107005419360377 1213 78-79 0.19234321641365426 1213 80-81 0.19545535284164828 1213 82-83 0.2140827376716885 1213 84-85 0.2971698866616421 1213 86-87 0.228330805923882 1213 88-89 0.136921845111047 1213 90-91 0.17089447261036383 1213 92-93 0.17368785958187516 1213 94-95 0.20574396081604363 1213 96-97 0.2608842273851977 1213 98-99 0.08506055503225696 1213 100-101 0.24426358931468783 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1896.0 3 325.0 4 367.0 5 415.0 6 1043.0 7 2456.0 8 7318.0 9 8212.0 10 7720.0 11 6916.0 12 5639.0 13 5159.0 14 5230.0 15 5742.0 16 5965.0 17 6436.0 18 7313.0 19 8215.0 20 9318.0 21 10810.0 22 12332.0 23 14149.0 24 16103.0 25 19547.0 26 23387.0 27 29038.0 28 35246.0 29 44039.0 30 56131.0 31 73305.0 32 101832.0 33 148428.0 34 227429.0 35 382455.0 36 760628.0 37 1423122.0 38 479595.0 39 2009.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.85622737770424 32.21092849141922 15.66294025114568 19.269903879730855 2 31.355223390184335 24.586598287599255 29.619573829186365 14.438604493030047 3 32.7155822015894 23.40371765905795 24.00568664257439 19.875013496778262 4 28.795597231644848 22.83634503929541 28.58849519283526 19.779562536224482 5 30.835286120737766 21.04238042883769 28.540247696065684 19.58208575435886 6 28.336986542191582 21.45523271822804 30.363953835058698 19.843826904521677 7 31.85858254365454 21.863049413609367 24.477155910474202 21.801212132261895 8 27.818195584030757 25.420348139240502 25.051713437289436 21.709742839439304 9 26.232864453073873 23.198218553744525 28.39230601949495 22.176610973686657 10-11 22.2524664508016 29.032231935475377 31.046740558940684 17.66856105478234 12-13 22.615653493093614 28.31122772043098 30.126570004033166 18.946548782442235 14-15 22.364239982530155 28.100325027771362 30.613792950663505 18.92164203903498 16-17 22.520478104514062 27.798296635893234 30.796658995144043 18.88456626444866 18-19 22.10668846019342 27.801213469442466 30.886134940222004 19.205963130142113 20-21 22.1823829889357 28.199928509623103 30.37036015049433 19.24732835094687 22-23 22.19843118426444 28.28696170086043 30.01650305421723 19.4981040606579 24-25 21.67995383153622 28.429764927606215 30.267272837541093 19.62300840331647 26-27 21.50438233887342 28.645234859787326 30.546767648931418 19.303615152407833 28-29 21.376132448713793 29.10234542063973 30.14648713845306 19.375034992193417 30-31 21.239033882287483 29.437728073121868 30.10835926071182 19.214878783878834 32-33 20.815140516363133 29.49828920719061 30.418347694792942 19.26822258165332 34-35 20.84670964337451 29.73696428163697 30.495690912734958 18.920635162253564 36-37 20.83008100609607 29.30188082390655 30.911513276471197 18.956524893526183 38-39 21.054496228714132 28.84699445095451 30.862008560137745 19.236500760193607 40-41 21.021691997083447 29.27597612972069 30.053203769600355 19.64912810359551 42-43 21.396132922679385 28.92769664823612 30.028619503203792 19.6475509258807 44-45 20.90643185842504 28.9751746601927 30.378011841884963 19.740381639497304 46-47 21.085623885171735 29.237286200360806 30.12180331321251 19.555286601254952 48-49 20.862835223572876 29.65859230488284 29.874686131772073 19.60388633977221 50-51 20.984717417292874 29.376074646791437 29.51703709642981 20.12217083948588 52-53 21.198638004188787 29.136349388990656 29.531837089713857 20.133175517106704 54-55 21.537453656197798 29.134805765086547 29.452803839670423 19.874936739045232 56-57 21.57109330790929 28.825229271156388 29.608690517522305 19.994986903412013 58-59 21.555957638012902 29.329074049763094 29.440439474857477 19.674528837366527 60-61 21.130728499059316 29.548560263401775 29.137235935005783 20.183475302533125 62-63 21.128806585666602 29.518621244924287 29.05478440310264 20.297787766306467 64-65 20.95729268179792 29.352418910496414 29.30752857795923 20.382759829746437 66-67 21.301692371808905 29.016938430174793 29.284191077525847 20.397178120490462 68-69 21.306672997095255 29.116572965671374 29.673300681915997 19.903453355317367 70-71 21.25419124103842 29.835537768270488 29.298457947871015 19.611813042820074 72-73 20.936994420231343 30.02689156642392 29.10023488758245 19.935879125762288 74-75 21.104367087412108 29.573250310808376 28.9763030087214 20.346079593058125 76-77 21.188514723606488 29.29048216878018 29.015940194068317 20.505062913545014 78-79 20.874050134995414 28.998739113043047 29.725007464526858 20.40220328743468 80-81 20.822331482756976 29.384958090683988 29.44389385881181 20.348816567747225 82-83 20.408412002231145 29.68938457530717 29.27855270704123 20.623650715420457 84-85 20.47966153474292 29.62513237943627 29.397283132015982 20.497922953804824 86-87 20.641456045516897 29.483612944069737 29.877994959701965 19.996936050711405 88-89 20.76435570281944 29.947673155513293 29.598967033415803 19.689004108251467 90-91 21.118669431287135 29.702498937903833 29.048526698244213 20.13030493256482 92-93 20.817963825253955 29.796579816372308 29.093496143189633 20.2919602151841 94-95 20.445207203252778 29.887428413703844 29.11429831704927 20.553066065994106 96-97 20.540248806167387 30.279744302910434 28.96784409624617 20.212162794676008 98-99 20.835291661794624 30.074446495845468 28.8187404995177 20.27152134284221 100-101 20.93120083479782 30.09114875124651 28.438154207032753 20.539496206922923 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1251.0 1 956.5 2 496.5 3 345.5 4 350.0 5 434.0 6 575.5 7 725.5 8 962.0 9 1184.5 10 1379.0 11 1448.0 12 1327.5 13 1135.0 14 933.0 15 765.5 16 634.5 17 575.5 18 586.0 19 647.0 20 853.5 21 1176.5 22 1603.5 23 2324.5 24 3370.0 25 5068.0 26 8151.0 27 12573.5 28 17555.5 29 22997.0 30 28901.5 31 33569.0 32 40892.0 33 56682.5 34 79424.5 35 112154.0 36 156037.0 37 208006.5 38 259412.0 39 279443.0 40 286219.5 41 275078.0 42 241530.5 43 226131.5 44 213160.0 45 203587.0 46 208614.0 47 195127.5 48 159843.0 49 129722.5 50 103634.5 51 83813.5 52 69165.0 53 55197.0 54 45020.0 55 34990.5 56 24059.0 57 15501.5 58 10296.0 59 7447.5 60 5594.0 61 4168.5 62 2944.5 63 2014.5 64 1492.5 65 1119.5 66 818.5 67 624.0 68 480.5 69 349.0 70 249.5 71 200.0 72 151.0 73 107.0 74 100.5 75 90.5 76 61.5 77 44.5 78 41.0 79 32.0 80 24.5 81 23.0 82 21.0 83 16.5 84 15.5 85 14.5 86 8.5 87 6.0 88 5.0 89 4.0 90 6.0 91 5.0 92 2.5 93 3.0 94 4.0 95 3.5 96 2.0 97 1.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3029123169846812 2 0.27768015837098353 3 0.24989444462704188 4 0.23737949621644036 5 0.24342206726721563 6 0.19212342014578018 7 0.1566517582870449 8 0.1543004649492955 9 0.1320011023267691 10-11 0.11766579778371641 12-13 0.13953535409719187 14-15 0.14177287517666304 16-17 0.1360336968146296 18-19 0.12291196302654432 20-21 0.12863850002654686 22-23 0.15938229248572158 24-25 0.5743097184263022 26-27 0.22836620508840105 28-29 0.2012757662561595 30-31 0.3045177699626068 32-33 0.3346927011303906 34-35 0.3438450472407699 36-37 0.3342628948213396 38-39 0.3415948847992678 40-41 0.3446540944107482 42-43 0.35589226525622775 44-45 0.33701871174407816 46-47 0.3559807547904441 48-49 0.3520998566469546 50-51 0.35773790411274076 52-53 0.34269468329595704 54-55 0.33537533468005976 56-57 0.3437059922584299 58-59 0.3360706095917599 60-61 0.313404647470337 62-63 0.2997899005630463 64-65 0.3116727808721023 66-67 0.32698147029153507 68-69 0.3137333228831407 70-71 0.2940127981149201 72-73 0.2899549209029978 74-75 0.2811312502054221 76-77 0.28434215616127345 78-79 0.2937220467882091 80-81 0.30324099239748487 82-83 0.32622298856968046 84-85 0.31683045658071385 86-87 0.3197253284857924 88-89 0.324592252867693 90-91 0.3184359095586395 92-93 0.2997519764769535 94-95 0.29556768564472213 96-97 0.31354370245267704 98-99 0.3212675746535635 100-101 0.32256963494274726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3955270.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.394406357339705 #Duplication Level Percentage of deduplicated Percentage of total 1 87.53180315342135 37.98390635230235 2 5.842266595862337 5.07043381417524 3 1.839971887807535 2.3953346335680488 4 0.9886598925329282 1.7160923650311073 5 0.6547992577410137 1.420731253644898 6 0.3769061246640007 0.9813370519343868 7 0.3597802852515131 1.0928716328294628 8 0.24513567355165114 0.8510013624624416 9 0.1756549258066213 0.6860197106208793 >10 1.5637064848128994 13.650032892664246 >50 0.2222167245627947 6.685190323783184 >100 0.1652323003890706 14.023799005171592 >500 0.023486731449755743 7.128152855967279 >1k 0.010379962146553401 6.315096745844786 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0010618744105964954 0.0 0.0 2 0.0012641362030910658 0.0 0.0 3 0.0015675288918329216 0.0 0.0 4 0.001972052476822063 0.0 0.0 5 0.0024018587858730253 0.0 0.0 6 0.0033878850242840563 0.0 0.0 7 0.004196932194262338 0.0 0.0 8 0.0054863511214152255 0.0 0.0 9 0.00614370194702258 0.0 0.0 10-11 0.00787556854525734 0.0 0.0 12-13 0.011099115863139558 0.0 0.0 14-15 0.01572585436645286 0.0 0.0 16-17 0.023272747498906524 0.0 0.0 18-19 0.03241245224725493 0.0 1.2641362030910658E-5 20-21 0.042411769613705255 0.0 2.5282724061821316E-5 22-23 0.053649940459184836 0.0 2.5282724061821316E-5 24-25 0.06766921095146475 0.0 2.5282724061821316E-5 26-27 0.08215621183888837 1.2641362030910658E-5 2.5282724061821316E-5 28-29 0.09910827832233957 2.5282724061821316E-5 2.5282724061821316E-5 30-31 0.11829786588526195 2.5282724061821316E-5 2.5282724061821316E-5 32-33 0.13714613667334974 2.5282724061821316E-5 2.5282724061821316E-5 34-35 0.15852267986761964 2.5282724061821316E-5 2.5282724061821316E-5 36-37 0.18187127553871163 2.5282724061821316E-5 3.7924086092731974E-5 38-39 0.20963170655859145 2.5282724061821316E-5 5.056544812364263E-5 40-41 0.2414626561524245 2.5282724061821316E-5 5.056544812364263E-5 42-43 0.27594829177274877 2.5282724061821316E-5 5.056544812364263E-5 44-45 0.31257031757629694 2.5282724061821316E-5 5.056544812364263E-5 46-47 0.35254230431803646 2.5282724061821316E-5 5.056544812364263E-5 48-49 0.3962687755829564 2.5282724061821316E-5 5.056544812364263E-5 50-51 0.4466698860001972 2.5282724061821316E-5 5.056544812364263E-5 52-53 0.4986132425852091 2.5282724061821316E-5 5.056544812364263E-5 54-55 0.5548799449847924 2.5282724061821316E-5 5.056544812364263E-5 56-57 0.6142817051680416 2.5282724061821316E-5 5.056544812364263E-5 58-59 0.6783607693027278 2.5282724061821316E-5 5.056544812364263E-5 60-61 0.7443613204661124 2.5282724061821316E-5 5.056544812364263E-5 62-63 0.8154184164418611 2.5282724061821316E-5 5.056544812364263E-5 64-65 0.8893830256847194 2.5282724061821316E-5 5.056544812364263E-5 66-67 0.966697595865769 2.5282724061821316E-5 5.056544812364263E-5 68-69 1.0499662475633773 2.5282724061821316E-5 5.056544812364263E-5 70-71 1.1398336902411215 2.5282724061821316E-5 5.056544812364263E-5 72-73 1.2359712484861969 2.5282724061821316E-5 5.056544812364263E-5 74-75 1.3376204405767496 2.5282724061821316E-5 5.056544812364263E-5 76-77 1.4471325598505285 2.5282724061821316E-5 5.056544812364263E-5 78-79 1.5634330905349065 2.5282724061821316E-5 7.584817218546395E-5 80-81 1.6908200957203934 2.5282724061821316E-5 7.584817218546395E-5 82-83 1.828686790029505 2.5282724061821316E-5 7.584817218546395E-5 84-85 1.9733039716631229 2.5282724061821316E-5 7.584817218546395E-5 86-87 2.130827983930301 2.5282724061821316E-5 7.584817218546395E-5 88-89 2.3009680755043274 2.5282724061821316E-5 7.584817218546395E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGG 40 7.5638236E-6 59.35517 1 CGGACGG 50 4.2544343E-7 56.980965 1 CGGAGCG 35 2.7933807E-4 54.267586 1 CGACGGG 150 0.0 47.48414 1 CGGTGCG 40 5.398665E-4 47.48414 1 CGACGCC 95 3.8744474E-10 44.984974 1 AACGCGG 75 1.3675162E-7 44.318527 1 CGGGAGA 425 0.0 42.44026 4 CGACGCG 45 9.643766E-4 42.20812 1 CGCGCAA 45 9.643766E-4 42.20812 1 CGAGGCG 150 0.0 41.15292 1 GTCGGGC 75 6.942457E-6 37.987312 1 GTGTCGC 50 0.0016198547 37.982975 2 ATTGACG 145 1.6370905E-11 36.02245 1 CGGAAAG 265 0.0 35.823 5 CGCAGCC 160 1.8189894E-12 35.613102 1 TCGGGAA 215 0.0 35.325382 3 AGTCCGG 95 8.7298395E-7 34.98831 1 GCGACTA 55 0.0025860593 34.529976 2 AGACGCG 140 4.5838533E-10 33.91724 1 >>END_MODULE