FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_44065-1.342000000357db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_44065-1.342000000357db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3955270
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG407.5638236E-659.355171
CGGACGG504.2544343E-756.9809651
CGGAGCG352.7933807E-454.2675861
CGACGGG1500.047.484141
CGGTGCG405.398665E-447.484141
CGACGCC953.8744474E-1044.9849741
AACGCGG751.3675162E-744.3185271
CGGGAGA4250.042.440264
CGACGCG459.643766E-442.208121
CGCGCAA459.643766E-442.208121
CGAGGCG1500.041.152921
GTCGGGC756.942457E-637.9873121
GTGTCGC500.001619854737.9829752
ATTGACG1451.6370905E-1136.022451
CGGAAAG2650.035.8235
CGCAGCC1601.8189894E-1235.6131021
TCGGGAA2150.035.3253823
AGTCCGG958.7298395E-734.988311
GCGACTA550.002586059334.5299762
AGACGCG1404.5838533E-1033.917241
Produced by FastQC (version 0.11.2)