FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_41442-1.3420000003572e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_41442-1.3420000003572e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199754
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT101610.3175556620915233Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC58970.1842954177102365No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG58410.18254528316864357No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC58050.18142019667761958No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52090.16279376477066676No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG50900.15907472886978186No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA39700.12407203803792416No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38980.12182186505587617No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA37080.11588390857547173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC26700.088.073779
ATTCGGA8150.086.203412
CGGGAGA36750.084.702594
CGGAAAG23900.083.983255
AGAGCGG29900.082.929058
TCGGAGA12800.082.2987444
GAGAGCG33000.081.176647
GTTCGGA8750.080.292322
AAGCGGC10750.079.826359
TTCGGAG8350.079.566933
AAGGGGC33350.077.762049
TCGGGAG40750.076.628323
ATCCGGA10100.074.730192
GATCCGG10950.074.146531
ACCGGAA5900.073.194833
CGGAGAG23150.072.1509255
GGATCGG18700.071.854451
TCGGAAA20300.071.761674
TTCGGAA10550.070.621713
AAAGCGG13900.069.9222268
Produced by FastQC (version 0.11.2)