##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_41442-1.3420000003572e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3199754 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.938376825218437 33.0 33.0 33.0 27.0 33.0 2 30.385646521576348 33.0 33.0 33.0 22.0 33.0 3 30.23554435747248 33.0 33.0 33.0 22.0 33.0 4 33.59655711032786 37.0 37.0 37.0 15.0 37.0 5 33.686021800425905 37.0 37.0 37.0 22.0 37.0 6 34.270693622072194 37.0 37.0 37.0 27.0 37.0 7 33.79631715438124 37.0 37.0 37.0 22.0 37.0 8 33.94828914972839 37.0 37.0 37.0 22.0 37.0 9 33.3962251473082 37.0 37.0 37.0 15.0 37.0 10-11 33.458781518829255 37.0 37.0 37.0 15.0 37.0 12-13 33.43441745834211 37.0 37.0 37.0 15.0 37.0 14-15 35.02443828494316 40.0 37.0 40.0 15.0 40.0 16-17 34.942815447687536 40.0 37.0 40.0 15.0 40.0 18-19 34.990718505235094 40.0 37.0 40.0 15.0 40.0 20-21 34.95806693264545 40.0 37.0 40.0 15.0 40.0 22-23 35.74456692608244 40.0 37.0 40.0 24.5 40.0 24-25 35.06689623639818 40.0 37.0 40.0 18.5 40.0 26-27 34.822736997906716 40.0 37.0 40.0 18.5 40.0 28-29 34.59109012755356 40.0 37.0 40.0 15.0 40.0 30-31 34.590033952610106 40.0 37.0 40.0 15.0 40.0 32-33 34.352207857229025 38.5 37.0 40.0 15.0 40.0 34-35 34.540521552594356 37.0 37.0 40.0 15.0 40.0 36-37 34.38267301173778 37.0 37.0 40.0 15.0 40.0 38-39 34.23470257401038 37.0 37.0 40.0 15.0 40.0 40-41 34.06939674112447 37.0 37.0 40.0 15.0 40.0 42-43 33.967737207297816 37.0 37.0 40.0 15.0 40.0 44-45 33.83048149951527 37.0 33.0 40.0 15.0 40.0 46-47 33.74948746059853 37.0 33.0 40.0 15.0 40.0 48-49 33.639083035758375 37.0 33.0 40.0 10.5 40.0 50-51 32.69669293326925 37.0 33.0 38.5 15.0 38.5 52-53 33.09449617064312 37.0 33.0 38.5 15.0 40.0 54-55 33.741329177180496 37.0 33.0 40.0 15.0 40.0 56-57 34.07768050293866 37.0 33.0 40.0 18.5 40.0 58-59 33.51833937858973 37.0 33.0 40.0 15.0 40.0 60-61 33.25507976550698 37.0 33.0 40.0 10.5 40.0 62-63 32.878980071593006 37.0 33.0 40.0 6.0 40.0 64-65 32.66557382223759 37.0 33.0 38.5 6.0 40.0 66-67 32.481798757029445 37.0 33.0 37.0 10.5 40.0 68-69 32.54980070342907 37.0 33.0 37.0 15.0 40.0 70-71 32.171040805011884 37.0 33.0 37.0 15.0 40.0 72-73 32.39486441770211 37.0 33.0 37.0 15.0 37.0 74-75 32.53695549720385 37.0 33.0 37.0 22.0 37.0 76-77 32.286192626058124 37.0 33.0 37.0 22.0 37.0 78-79 31.85773890742851 37.0 33.0 37.0 22.0 37.0 80-81 31.322592143020994 37.0 33.0 37.0 15.0 37.0 82-83 30.974318494484265 33.0 33.0 37.0 15.0 37.0 84-85 30.67403306629197 33.0 33.0 37.0 15.0 37.0 86-87 30.512968028167165 33.0 27.0 37.0 15.0 37.0 88-89 30.399020518452353 33.0 27.0 37.0 15.0 37.0 90-91 30.265338679160962 33.0 27.0 37.0 10.5 37.0 92-93 30.09835146701903 33.0 27.0 37.0 6.0 37.0 94-95 29.95961002002029 33.0 27.0 37.0 6.0 37.0 96-97 29.824840597120904 33.0 27.0 37.0 6.0 37.0 98-99 29.69029416011356 33.0 27.0 37.0 6.0 37.0 100-101 28.47972187861942 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.025989014117033804 2306 2 0.046908650665979934 2306 3 0.03261366390965392 2306 4 0.14474193723656725 2306 5 0.034252077098400946 2306 6 -0.05992962758888609 2306 7 0.22683281464335892 2306 8 -0.10492640091789696 2306 9 0.2939588127675279 2306 10-11 0.1568106867850716 2306 12-13 0.13590047284452567 2306 14-15 0.443122935201373 2306 16-17 0.4140969799932108 2306 18-19 0.3656587622182883 2306 20-21 0.42368888813157923 2306 22-23 0.26644801543222485 2306 24-25 0.459436949302642 2306 26-27 0.0683114512108034 2306 28-29 0.17841946102104345 2306 30-31 0.19684844564375226 2306 32-33 0.2905794787329299 2306 34-35 0.28332902520764947 2306 36-37 0.4284265731555834 2306 38-39 0.3257251652563937 2306 40-41 0.28342315584757927 2306 42-43 0.33982709109427844 2306 44-45 0.40102300585106576 2306 46-47 0.3355470780323273 2306 48-49 0.4394150580907876 2306 50-51 0.413353446435508 2306 52-53 0.2909991187207268 2306 54-55 0.4560033676760966 2306 56-57 0.26388347055816297 2306 58-59 0.4997333217098543 2306 60-61 0.33740759351381655 2306 62-63 0.4957230388494267 2306 64-65 0.363153642811227 2306 66-67 0.3743510250892399 2306 68-69 0.1843309546985168 2306 70-71 0.4815232152123059 2306 72-73 0.0853068234634975 2306 74-75 0.13400304826367204 2306 76-77 0.28975767322460655 2306 78-79 0.35718218818222525 2306 80-81 0.6489145914079835 2306 82-83 0.6365584839993268 2306 84-85 0.7464665024533055 2306 86-87 0.6286708472359592 2306 88-89 0.452713761719572 2306 90-91 0.394248149014679 2306 92-93 0.3780783400410641 2306 94-95 0.4217971869169439 2306 96-97 0.3528662953998172 2306 98-99 0.3304755281257137 2306 100-101 0.4642935340394594 2307 1 -0.03880174342220499 2307 2 0.13052625564615994 2307 3 0.04478639085606062 2307 4 0.09404070193430414 2307 5 0.020669803096154737 2307 6 -0.14804703514632678 2307 7 0.212410186408313 2307 8 -0.20110460442227662 2307 9 0.13293241965527614 2307 10-11 0.057108513083825585 2307 12-13 0.04975216478301547 2307 14-15 0.4228075716102282 2307 16-17 0.32109856450296803 2307 18-19 0.3157158353364906 2307 20-21 0.19360156062008116 2307 22-23 0.06385731635062797 2307 24-25 0.2236340028324051 2307 26-27 -0.07443462212790308 2307 28-29 0.032729276264127805 2307 30-31 0.14740548543083776 2307 32-33 0.23677650149551255 2307 34-35 0.055009374722807536 2307 36-37 0.35658038854224827 2307 38-39 0.1449111682408173 2307 40-41 0.11001369083755463 2307 42-43 0.07796383069625534 2307 44-45 0.2246112794533346 2307 46-47 0.21898551546983214 2307 48-49 0.07565448828262333 2307 50-51 -0.07992074554143613 2307 52-53 0.022014589484207647 2307 54-55 0.10651835898436701 2307 56-57 -0.008227414357200757 2307 58-59 0.26722389926109 2307 60-61 0.24919214121064215 2307 62-63 0.3662331074089522 2307 64-65 0.28792029939309316 2307 66-67 0.28237264416078744 2307 68-69 0.05274885504362459 2307 70-71 0.08644719463931239 2307 72-73 -0.03564901025014677 2307 74-75 -0.10623201298865581 2307 76-77 0.12531017485270723 2307 78-79 0.1734595645622683 2307 80-81 0.3734857738419741 2307 82-83 0.5032650112564845 2307 84-85 0.36481830464085263 2307 86-87 0.22427965280276752 2307 88-89 0.3227396574290893 2307 90-91 0.2555724709829619 2307 92-93 0.300312652625351 2307 94-95 0.2138830478595466 2307 96-97 0.26044732845915064 2307 98-99 0.20672562427314745 2307 100-101 0.31225628524066096 2201 1 0.0345373079284883 2201 2 0.11368713533920172 2201 3 0.015852530306414536 2201 4 0.07903921890226684 2201 5 0.009463181783189611 2201 6 -0.1428214725859931 2201 7 0.2879664178822239 2201 8 -0.10388533671893896 2201 9 0.10719189547429409 2201 10-11 0.06027511709160649 2201 12-13 0.06934755208108356 2201 14-15 0.42604369864844926 2201 16-17 0.22638732123092353 2201 18-19 0.2556182763883328 2201 20-21 0.2316067597336655 2201 22-23 0.21239364874628563 2201 24-25 0.45262954617944473 2201 26-27 0.08865847261045445 2201 28-29 0.1327340937567243 2201 30-31 0.345281065655314 2201 32-33 0.35877496745472826 2201 34-35 0.08116014145981865 2201 36-37 0.3026197484013906 2201 38-39 0.15545911551666336 2201 40-41 0.2220003681090006 2201 42-43 0.20236613100173884 2201 44-45 0.29012653390196164 2201 46-47 0.27432093575355054 2201 48-49 0.30140464744879836 2201 50-51 0.23623372405263154 2201 52-53 0.09780073815392143 2201 54-55 0.2580507939340535 2201 56-57 0.32511539652693244 2201 58-59 0.6392474917503392 2201 60-61 0.41732083816390286 2201 62-63 0.4642250631409226 2201 64-65 0.4548598313595136 2201 66-67 0.349053165054535 2201 68-69 0.32194807442090223 2201 70-71 0.47037226090345285 2201 72-73 0.1768742034519306 2201 74-75 0.248849780478821 2201 76-77 0.3724933585918748 2201 78-79 0.6464823616929287 2201 80-81 0.6339059158729903 2201 82-83 0.6345168414313669 2201 84-85 0.42713162680264105 2201 86-87 0.3789484643556804 2201 88-89 0.5952123157970703 2201 90-91 0.4182620298706681 2201 92-93 0.4977081838814321 2201 94-95 0.481149413637592 2201 96-97 0.35124686220143886 2201 98-99 0.3636623413125264 2201 100-101 0.25016976307358263 1101 1 0.06129320145512196 1101 2 -0.06086730219471903 1101 3 -0.02748945331210706 1101 4 -0.03097556559958292 1101 5 0.008481915057053868 1101 6 -0.036393333374626025 1101 7 0.23306725816593143 1101 8 -0.12515193809258562 1101 9 0.18336483047863084 1101 10-11 0.07585841871415511 1101 12-13 0.10320063031417703 1101 14-15 0.3718098415153932 1101 16-17 0.22167795833690462 1101 18-19 0.3155989068194458 1101 20-21 0.31930412035698197 1101 22-23 0.1913915464774547 1101 24-25 0.23482435850360872 1101 26-27 0.1216480298863587 1101 28-29 0.18171040736283572 1101 30-31 0.1602433558183236 1101 32-33 0.08766307310465749 1101 34-35 0.08180766521171279 1101 36-37 0.16545996349406522 1101 38-39 0.0725894873749553 1101 40-41 0.16944941772130306 1101 42-43 0.2242910169988832 1101 44-45 0.23371040319948122 1101 46-47 0.21911080244350245 1101 48-49 0.12210575722216532 1101 50-51 0.1650366784577244 1101 52-53 0.06354623262377146 1101 54-55 0.30453136510239887 1101 56-57 0.35843713723047443 1101 58-59 0.4493730040613997 1101 60-61 0.28908930163620283 1101 62-63 0.41852972369000696 1101 64-65 0.33025408474217244 1101 66-67 0.25313512234791347 1101 68-69 0.2512731650305611 1101 70-71 0.3594413987237104 1101 72-73 -0.005564677916474636 1101 74-75 0.06480690542346679 1101 76-77 0.24882911026215027 1101 78-79 0.44954702673350866 1101 80-81 0.5022650545042069 1101 82-83 0.42741965672490423 1101 84-85 0.4063218166306548 1101 86-87 0.43908551731164636 1101 88-89 0.4553506163903265 1101 90-91 0.36288408934553473 1101 92-93 0.4584533040600931 1101 94-95 0.42475558445161 1101 96-97 0.34820231531210766 1101 98-99 0.316616590453215 1101 100-101 0.26772620306534733 2304 1 -0.0010902224358844137 2304 2 -0.22576088883825207 2304 3 -0.13559298489002813 2304 4 -0.22744421612082277 2304 5 -0.22730479449553087 2304 6 -0.1440759630917796 2304 7 0.008699724605151005 2304 8 -0.1532324314329685 2304 9 -0.19060704391100103 2304 10-11 -0.2404633216379395 2304 12-13 -0.1651994057794468 2304 14-15 -0.20902216210310343 2304 16-17 -0.16062014868981578 2304 18-19 -0.035506203283219406 2304 20-21 -0.19231979554945156 2304 22-23 -0.10405443245345936 2304 24-25 -0.12536554990311544 2304 26-27 -0.3046071942653015 2304 28-29 -0.24889047144827003 2304 30-31 -0.10564554665009496 2304 32-33 -0.17718899445437586 2304 34-35 -0.2167699492056343 2304 36-37 -0.07013499870472373 2304 38-39 -0.22301211673514842 2304 40-41 -0.2549939866837221 2304 42-43 -0.2471406261403004 2304 44-45 -0.2085733577556752 2304 46-47 -0.20342041111307907 2304 48-49 -0.2485525858294082 2304 50-51 -0.12137590131089127 2304 52-53 -0.22550997633544512 2304 54-55 0.018679749855813554 2304 56-57 0.10154808170683793 2304 58-59 -0.05418924201256203 2304 60-61 -0.1917685157734894 2304 62-63 -0.03961823959044608 2304 64-65 -0.015984668840062 2304 66-67 0.025733897220817425 2304 68-69 -0.0683752419958168 2304 70-71 -0.1996938849595793 2304 72-73 -0.11006198859916339 2304 74-75 0.16960304500526036 2304 76-77 0.22417836743032638 2304 78-79 0.24467190380389425 2304 80-81 0.1541659375414639 2304 82-83 -0.010188638411250395 2304 84-85 0.06807648624268126 2304 86-87 -0.01100705825365722 2304 88-89 -0.08654625180285791 2304 90-91 -0.09438266420471564 2304 92-93 -0.14105272163262583 2304 94-95 -0.11347338387623651 2304 96-97 -0.12169320736576239 2304 98-99 -0.14562088843030097 2304 100-101 -0.14785156326501792 2202 1 0.13268558602761615 2202 2 -0.1701494663143137 2202 3 -0.08913322733140205 2202 4 -0.05415099862135264 2202 5 -0.04373250641147308 2202 6 0.06823186072824683 2202 7 0.08269524015558716 2202 8 0.18095503282345504 2202 9 0.14036392516485563 2202 10-11 0.06028822092408603 2202 12-13 0.0985601477999225 2202 14-15 0.1789060625798271 2202 16-17 0.006906505098463356 2202 18-19 0.11073944029507743 2202 20-21 0.20482689409449506 2202 22-23 0.17166350033642885 2202 24-25 0.023895992205261507 2202 26-27 0.09835043945990662 2202 28-29 0.010102486167106406 2202 30-31 0.07419957700265911 2202 32-33 -0.05616490528772289 2202 34-35 -0.04830121225916173 2202 36-37 0.11321781304410194 2202 38-39 0.048295901912737804 2202 40-41 -0.0011265357059997427 2202 42-43 0.02624238785976729 2202 44-45 0.06731927767263102 2202 46-47 0.09925115605546608 2202 48-49 0.17383604566352773 2202 50-51 -0.007845277020024355 2202 52-53 0.10618890947471726 2202 54-55 0.2457751523117011 2202 56-57 0.32426379062719946 2202 58-59 0.24256426508554796 2202 60-61 0.10541102976628025 2202 62-63 0.1546727272932813 2202 64-65 0.21688077171344844 2202 66-67 0.3110826980854995 2202 68-69 0.14236871460398248 2202 70-71 0.17155131940383228 2202 72-73 0.21246005933269174 2202 74-75 0.3491810997841398 2202 76-77 0.23129240308618648 2202 78-79 0.24413298058049193 2202 80-81 0.20001990632652777 2202 82-83 0.23762302257359025 2202 84-85 0.2701702695033781 2202 86-87 0.3042207448092036 2202 88-89 0.22094179827445615 2202 90-91 0.22636607000419673 2202 92-93 0.25959378957207235 2202 94-95 0.16752056859363051 2202 96-97 0.3372531148060922 2202 98-99 0.32565249124041884 2202 100-101 0.3621141569962667 1102 1 -0.10881405428684943 1102 2 -0.3823419219507933 1102 3 -0.2543258909251769 1102 4 -0.37419944100437164 1102 5 -0.439970415811608 1102 6 -0.15133712522555243 1102 7 -0.22530539057203214 1102 8 -0.08087462814120272 1102 9 -0.32240805056295585 1102 10-11 -0.29127507308579226 1102 12-13 -0.2958581051976239 1102 14-15 -0.34601068534333734 1102 16-17 -0.4285923945560697 1102 18-19 -0.3236232716268219 1102 20-21 -0.28100242191844416 1102 22-23 -0.07569971782736928 1102 24-25 -0.29056839387509115 1102 26-27 -0.08428140985536459 1102 28-29 -0.24698066078846637 1102 30-31 -0.17075000147186614 1102 32-33 -0.2849007907425829 1102 34-35 -0.2450537107235391 1102 36-37 -0.21668845462519926 1102 38-39 -0.387131192938277 1102 40-41 -0.40958587524373513 1102 42-43 -0.405857751095148 1102 44-45 -0.3153213638221004 1102 46-47 -0.22912868822793797 1102 48-49 -0.2477380743938582 1102 50-51 -0.17295591234579888 1102 52-53 -0.08391731472682551 1102 54-55 -0.031260589040421394 1102 56-57 0.1455846935106635 1102 58-59 -0.20089859312810887 1102 60-61 -0.2677966615986378 1102 62-63 -0.13855938732691442 1102 64-65 -0.13488380427077118 1102 66-67 -0.05497614553001284 1102 68-69 0.09797085441118725 1102 70-71 -0.08910955901788498 1102 72-73 0.09884778097589475 1102 74-75 0.372101325369087 1102 76-77 0.22398876005684798 1102 78-79 0.2376982718945051 1102 80-81 0.05085083945099811 1102 82-83 -0.09269380740189703 1102 84-85 -0.04612411672011163 1102 86-87 -0.04014547075410846 1102 88-89 -0.10956669159114085 1102 90-91 -0.13199215946976395 1102 92-93 -0.08766680906348867 1102 94-95 0.09615284825662584 1102 96-97 0.058045067664611594 1102 98-99 -0.07716626224983969 1102 100-101 0.07473148778921157 2305 1 0.16606329068288872 2305 2 -0.012468021555090303 2305 3 0.01352088354828851 2305 4 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0.36774048053298714 1213 48-49 0.3377861981239434 1213 50-51 0.2703239991602544 1213 52-53 0.2044771304668913 1213 54-55 -0.014438443771297216 1213 56-57 0.07015861533889733 1213 58-59 -0.033133746045486134 1213 60-61 0.15767096143081716 1213 62-63 0.101633366882659 1213 64-65 0.19170598897530056 1213 66-67 0.3648339685889397 1213 68-69 0.4796584763388907 1213 70-71 0.28496515967380276 1213 72-73 0.4902308519489793 1213 74-75 0.29715476619192316 1213 76-77 0.2017399414219625 1213 78-79 0.18529702027750616 1213 80-81 0.0822952470651721 1213 82-83 0.09502836142171844 1213 84-85 0.23470519113892152 1213 86-87 0.3327083879075765 1213 88-89 0.22378733898030134 1213 90-91 0.30179741539075877 1213 92-93 0.26161416176124064 1213 94-95 0.2849675779783958 1213 96-97 0.3747847168294136 1213 98-99 0.14553993764543094 1213 100-101 0.26253217156580533 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1453.0 3 484.0 4 445.0 5 403.0 6 974.0 7 5133.0 8 29673.0 9 84178.0 10 94598.0 11 51007.0 12 25370.0 13 15733.0 14 11708.0 15 10708.0 16 10300.0 17 9432.0 18 9315.0 19 9624.0 20 10474.0 21 11096.0 22 12445.0 23 13507.0 24 15199.0 25 17323.0 26 20543.0 27 24938.0 28 30908.0 29 38380.0 30 45944.0 31 58223.0 32 79907.0 33 115548.0 34 175432.0 35 295529.0 36 581536.0 37 1007182.0 38 274757.0 39 345.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.258075395326635 22.947139647129585 23.61268568229752 13.182099275246257 2 31.770324942757345 24.781879389165148 31.8073325125445 11.640463155533007 3 36.71088789561334 20.035543642932062 28.661283430635077 14.592285030819527 4 27.52059659491348 17.870502217998457 36.023477393079695 18.585423794008367 5 33.106947176250095 16.197558337429083 33.55865443861903 17.13684004770179 6 30.107074412944236 16.520530866667478 38.27893825414135 15.093456466246934 7 36.65074501857734 18.19452829887503 28.745006683837133 16.409719998710493 8 28.053467905516953 32.03004087805345 24.17592116460357 15.740570051826023 9 32.34958375733635 27.618993082023145 23.110761478121443 16.920661682519057 10-11 27.038219340474917 29.776791818056953 26.82884629884967 16.356142542618457 12-13 29.551276925958337 23.908781771375185 26.896550213414265 19.64339108925221 14-15 26.222044540969524 24.32089338551112 28.933010169035374 20.524051904483983 16-17 27.155277283489692 25.620201813483135 29.78351824740544 17.441002655621734 18-19 26.87178928796093 25.76868965480558 30.19490215937569 17.164618897857803 20-21 25.687088461519807 27.909057926062747 29.19318862208699 17.210664990330454 22-23 28.65242146783265 25.6841448295268 28.401255625761173 17.26217807687938 24-25 25.61822692298506 28.917794124856538 28.183322627561058 17.28065632459734 26-27 22.547546148457773 31.85353517083069 28.13170020419595 17.467218476515587 28-29 23.77733494448388 30.147997057844545 27.905120183418152 18.169547814253423 30-31 26.643232570061333 27.062244122186 28.040533286860082 18.25399002089259 32-33 23.600548317673088 28.87791779749871 29.91621914145392 17.605314743374286 34-35 27.294182241437483 27.038234855126724 28.5495865332636 17.117996370172197 36-37 24.918167144704316 27.352098871647208 29.663422406776384 18.066311576872092 38-39 21.221167909646894 29.657295543222524 31.196956330262687 17.9245802168679 40-41 21.671888659912778 31.076211416723844 28.958670160259587 18.293229763103795 42-43 24.45166640820427 27.670409170799843 30.200570477991313 17.677353943004572 44-45 22.58922353801385 28.870262123292417 30.94350401347975 17.597010325213983 46-47 23.97687493286941 29.82514294405562 28.51448727125619 17.68349485181878 48-49 21.23999565989916 30.050227886653126 29.77102204984468 18.938754403603035 50-51 19.962059642693283 27.66028609249758 31.82390944993381 20.55374481487533 52-53 24.778262697069994 26.96322154477759 28.31983539396596 19.93868036418646 54-55 27.234702512581404 26.939759436563726 27.506146084455867 18.319391966399003 56-57 27.964108836186792 26.852273566412777 26.799610490785685 18.384007106614742 58-59 26.436323372111453 26.66661859051488 27.531064379349683 19.365993658023985 60-61 22.321798715190003 26.969082525540173 27.38909787605268 23.320020883217143 62-63 24.435561999559326 26.90163146754977 27.36524870375542 21.297557829135485 64-65 22.404609590220858 28.112228049583436 29.490503550148684 19.992658810047022 66-67 20.66621542100186 28.070181763802047 28.839833601332295 22.4237692138638 68-69 20.236316684670136 27.26128999293445 33.244486267940516 19.257907054454904 70-71 19.436153443663063 31.292548347804257 30.844168309326907 18.42712989920577 72-73 19.24210645077633 35.268673310209685 26.948705151579492 18.54051508743449 74-75 19.920490706340672 35.12777126568849 26.28217432158802 18.66956370638282 76-77 20.182407369250196 34.57328831699383 26.554788099428094 18.689516214327874 78-79 19.97834962256792 34.5054770785091 27.069302676382172 18.446870622540807 80-81 19.199424022607804 34.70362521599936 26.99820367520968 19.098747086183156 82-83 18.762131847464342 33.99193370773712 27.253088737209392 19.992845707589147 84-85 18.911994372384687 32.990672208806096 27.75240060019119 20.344932818618027 86-87 19.143612202225142 32.698540084203 28.420723468687203 19.73712424488465 88-89 19.630891025290342 33.01529233380009 27.797336174112242 19.556480466797325 90-91 20.055201483533992 32.294905516558956 27.432823800870892 20.21706919903616 92-93 19.68582442488017 32.7258440581111 27.478499512146808 20.109832004861918 94-95 19.345669275174224 33.107992588969736 27.58518289144577 19.961155244410268 96-97 19.913000192469127 33.45964511702061 26.87793758357831 19.74941710693196 98-99 20.583161303570225 32.2912169988918 26.5376639377025 20.58795775983548 100-101 20.51884496645926 32.3532283991595 26.067225692074135 21.060700942307104 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1363.0 1 1015.0 2 509.5 3 461.0 4 551.5 5 544.0 6 650.0 7 877.0 8 1126.0 9 1312.5 10 1436.0 11 1478.5 12 1408.5 13 1267.0 14 1166.5 15 1088.0 16 1007.5 17 1022.5 18 1064.5 19 1063.0 20 1116.0 21 1250.0 22 1460.5 23 1811.5 24 2274.0 25 3042.5 26 4621.5 27 7214.0 28 9758.5 29 11922.0 30 14325.0 31 16669.5 32 20120.5 33 29792.5 34 47801.0 35 78700.5 36 118593.5 37 160443.0 38 206887.0 39 237694.5 40 242188.5 41 220283.5 42 193098.5 43 186959.0 44 180124.5 45 167608.5 46 164839.0 47 151699.5 48 123333.0 49 101897.5 50 86056.0 51 74579.5 52 65243.0 53 54084.0 54 43614.5 55 34104.5 56 26056.5 57 19599.5 58 15076.5 59 11899.0 60 9321.0 61 7349.0 62 5667.0 63 4364.0 64 3458.5 65 2711.0 66 2098.0 67 1658.5 68 1279.0 69 942.5 70 722.5 71 557.5 72 417.0 73 330.5 74 264.0 75 211.5 76 164.5 77 115.0 78 96.5 79 86.5 80 63.5 81 54.5 82 39.0 83 30.5 84 31.5 85 31.0 86 25.5 87 19.0 88 11.5 89 8.0 90 9.0 91 7.5 92 6.5 93 7.0 94 5.0 95 5.0 96 5.5 97 4.0 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29036607189177666 2 0.2661142075297039 3 0.238205812071803 4 0.22614238469582348 5 0.23192407916358568 6 0.18148270148267648 7 0.14791762116712723 8 0.14504240013451034 9 0.12569716296940328 10-11 0.11078976696333531 12-13 0.13152573604095816 14-15 0.13341650639392902 16-17 0.12752542851731727 18-19 0.11608704919190663 20-21 0.12174373404955506 22-23 0.15166790947054057 24-25 0.5522299526776121 26-27 0.21837616266750506 28-29 0.1930460904182009 30-31 0.2934131811383 32-33 0.32383739499974057 34-35 0.33196301965713615 36-37 0.3227123085087166 38-39 0.3305722877446204 40-41 0.33371315419872905 42-43 0.344542111674835 44-45 0.3243061810376673 46-47 0.343979568429323 48-49 0.34027615872970235 50-51 0.34580783397723697 52-53 0.33013475410922216 54-55 0.32268105610618814 56-57 0.33166612183311595 58-59 0.32407178801870395 60-61 0.30103876735524043 62-63 0.28724083163893227 64-65 0.29800728430998136 66-67 0.3145554314487926 68-69 0.30089813154386247 70-71 0.2826936070710436 72-73 0.27928709519544315 74-75 0.27128648014816137 76-77 0.2739116819605507 78-79 0.28275611187610045 80-81 0.29224121604348335 82-83 0.31441479563741465 84-85 0.3053515989041658 86-87 0.30764865049000645 88-89 0.31310219473122 90-91 0.30638292818760443 92-93 0.28892846137546824 94-95 0.2845374988202218 96-97 0.3010231411539762 98-99 0.30936753262907085 100-101 0.3117895938250253 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3199754.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.16940817143747 #Duplication Level Percentage of deduplicated Percentage of total 1 89.17942063880967 42.95719913243137 2 5.10005813910615 4.91333564401332 3 1.5057342018982722 2.175909761067945 4 0.9490663137934674 1.8286385060351635 5 0.5144839183400101 1.2391192930080226 6 0.36475926712219986 1.0542142813394182 7 0.2902692333170129 0.9787468025480186 8 0.27721273302707605 1.0682538630000762 9 0.23150074816747937 1.0036128627425223 >10 1.2716071255284953 12.173675715768182 >50 0.15771779084161186 5.266886881996314 >100 0.13359930488753063 14.034169053881126 >500 0.01897336916853875 6.241666879421868 >1k 0.005207082563835378 3.8742859542699666 >5k 3.251111999648502E-4 0.8720344896526077 >10k+ 6.502222847840312E-5 0.31825087882397274 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 10161 0.3175556620915233 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5897 0.1842954177102365 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5841 0.18254528316864357 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5805 0.18142019667761958 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5209 0.16279376477066676 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5090 0.15907472886978186 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3970 0.12407203803792416 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3898 0.12182186505587617 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 3708 0.11588390857547173 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004687860379266656 0.0 0.0 2 0.005312908429835544 0.0 0.0 3 0.006031713687989764 0.0 0.0 4 0.0066567617385586515 0.0 0.0 5 0.00771934342452576 0.0 0.0 6 0.00871942030543598 0.0 0.0 7 0.010250788029329754 0.0 0.0 8 0.011657146143109752 0.0 0.0 9 0.013251018672060414 0.0 0.0 10-11 0.022236084398988172 0.0 0.0 12-13 0.030971130905688374 0.0 0.0 14-15 0.041565695362831015 0.0 0.0 16-17 0.053285346310997656 0.0 0.0 18-19 0.0652862688819203 0.0 0.0 20-21 0.0745838586341325 0.0 0.0 22-23 0.08410021520404382 0.0 0.0 24-25 0.09530420151049113 0.0 0.0 26-27 0.1073363764839422 0.0 0.0 28-29 0.12047801174715306 0.0 0.0 30-31 0.21389144290467332 0.0 0.0 32-33 0.2311583953016388 0.0 0.0 34-35 0.24465943319392677 0.0 0.0 36-37 0.2575823016394385 0.0 0.0 38-39 0.2719584068025229 0.0 0.0 40-41 0.2882877871236351 0.0 0.0 42-43 0.30472655085359684 0.0 0.0 44-45 0.32268105610618814 0.0 0.0 46-47 0.3412762356106126 0.0 0.0 48-49 0.3614965400465161 0.0 0.0 50-51 0.38304507158987844 0.0 0.0 52-53 0.4082032556252762 0.0 0.0 54-55 0.43483030257951083 0.0 0.0 56-57 0.46448883257900453 0.0 0.0 58-59 0.49755387445409865 0.0 0.0 60-61 0.53238467707205 0.0 0.0 62-63 0.5689968666341225 0.0 0.0 64-65 0.6088593060591534 0.0 0.0 66-67 0.6504718800257769 0.0 0.0 68-69 0.6962253973274195 0.0 0.0 70-71 0.7465730178007435 0.0 0.0 72-73 0.8006865527787448 0.0 0.0 74-75 0.8588003952803871 0.0 0.0 76-77 0.9211176859221053 0.0 0.0 78-79 0.9907324125542152 0.0 0.0 80-81 1.066456983880636 0.0 0.0 82-83 1.1500571606442245 0.0 0.0 84-85 1.2391421340515552 0.0 0.0 86-87 1.3331181084545873 0.0 0.0 88-89 1.4384543311767093 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2670 0.0 88.07377 9 ATTCGGA 815 0.0 86.20341 2 CGGGAGA 3675 0.0 84.70259 4 CGGAAAG 2390 0.0 83.98325 5 AGAGCGG 2990 0.0 82.92905 8 TCGGAGA 1280 0.0 82.298744 4 GAGAGCG 3300 0.0 81.17664 7 GTTCGGA 875 0.0 80.29232 2 AAGCGGC 1075 0.0 79.82635 9 TTCGGAG 835 0.0 79.56693 3 AAGGGGC 3335 0.0 77.76204 9 TCGGGAG 4075 0.0 76.62832 3 ATCCGGA 1010 0.0 74.73019 2 GATCCGG 1095 0.0 74.14653 1 ACCGGAA 590 0.0 73.19483 3 CGGAGAG 2315 0.0 72.150925 5 GGATCGG 1870 0.0 71.85445 1 TCGGAAA 2030 0.0 71.76167 4 TTCGGAA 1055 0.0 70.62171 3 AAAGCGG 1390 0.0 69.922226 8 >>END_MODULE