FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_40825-1.342000000356f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_40825-1.342000000356f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886966
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77270.4094933348030648No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76810.40705555902968044No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA48770.25845722710425095No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC40840.21643209257612483No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT27410.14525963901840308Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC10000.065.413169
CGGGAGA15800.055.2449534
AGAGCGG13050.052.67048
TCGGGAG17300.047.7153473
GGAGAGC13350.047.240866
ATCGGGA20000.046.031332
ATTGGGA8700.042.5458452
AAGGGGC12550.040.791169
TGTGTTT427200.040.238811
AAGCGGC3950.039.6007049
CGGAAAG7800.039.532175
GAGGGGC24100.039.1401189
GATTGGG10650.038.770161
GATCGGG24800.038.6569561
TTGTGTT239400.038.380371
GAACCGG3550.034.7594531
CGGAGAG12450.033.911935
TTGGGAG11550.033.6811373
TGGGAGA13450.033.5068174
TCGGAGA6500.032.8421064
Produced by FastQC (version 0.11.2)