##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_40255-1.342000000357f5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7809676 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.769493766450747 33.0 33.0 33.0 27.0 33.0 2 31.60165761037974 33.0 33.0 33.0 27.0 33.0 3 31.54645532029754 33.0 33.0 33.0 27.0 33.0 4 35.19676783006107 37.0 37.0 37.0 33.0 37.0 5 35.23548070368092 37.0 37.0 37.0 33.0 37.0 6 35.41261122740559 37.0 37.0 37.0 33.0 37.0 7 35.234084615033964 37.0 37.0 37.0 33.0 37.0 8 35.229180186220276 37.0 37.0 37.0 33.0 37.0 9 35.037607321993896 37.0 37.0 37.0 33.0 37.0 10-11 35.02815628971035 37.0 37.0 37.0 33.0 37.0 12-13 35.009831585842996 37.0 37.0 37.0 33.0 37.0 14-15 36.716879099721936 40.0 37.0 40.0 33.0 40.0 16-17 36.66644410088203 40.0 37.0 40.0 33.0 40.0 18-19 36.653842489752456 40.0 37.0 40.0 33.0 40.0 20-21 36.63179727302388 40.0 37.0 40.0 33.0 40.0 22-23 36.820672586161066 40.0 37.0 40.0 33.0 40.0 24-25 36.445148876854816 40.0 37.0 40.0 33.0 40.0 26-27 36.35166215602286 40.0 37.0 40.0 33.0 40.0 28-29 36.257097349493115 40.0 37.0 40.0 33.0 40.0 30-31 36.25214419906793 40.0 37.0 40.0 33.0 40.0 32-33 36.14877460985578 40.0 37.0 40.0 33.0 40.0 34-35 36.08894248621837 40.0 37.0 40.0 30.0 40.0 36-37 35.983162105572625 40.0 37.0 40.0 27.0 40.0 38-39 35.86900807408655 40.0 37.0 40.0 27.0 40.0 40-41 35.807702201730265 37.0 37.0 40.0 27.0 40.0 42-43 35.68637373688742 37.0 37.0 40.0 27.0 40.0 44-45 35.541603582530186 37.0 37.0 40.0 27.0 40.0 46-47 35.41806856007855 37.0 37.0 40.0 27.0 40.0 48-49 35.30024440194446 37.0 37.0 40.0 27.0 40.0 50-51 34.18365422586033 37.0 35.0 38.5 24.5 38.5 52-53 34.60742776012731 37.0 35.0 38.5 27.0 40.0 54-55 35.307615578418364 37.0 37.0 40.0 27.0 40.0 56-57 35.42498651672618 37.0 37.0 40.0 27.0 40.0 58-59 35.1440726222189 37.0 37.0 40.0 27.0 40.0 60-61 34.91473634757703 37.0 35.0 40.0 27.0 40.0 62-63 34.63096151492072 37.0 33.0 40.0 27.0 40.0 64-65 34.40751344870132 37.0 33.0 40.0 27.0 40.0 66-67 34.13663953536613 37.0 33.0 37.0 27.0 40.0 68-69 33.99818571218576 37.0 33.0 37.0 27.0 40.0 70-71 33.68065429346877 37.0 33.0 37.0 27.0 40.0 72-73 33.57374377631031 37.0 33.0 37.0 27.0 38.5 74-75 33.423324603991254 37.0 33.0 37.0 27.0 37.0 76-77 33.16067785654616 37.0 33.0 37.0 24.5 37.0 78-79 32.8786905500305 37.0 33.0 37.0 22.0 37.0 80-81 32.58200999631739 37.0 33.0 37.0 22.0 37.0 82-83 32.32885314320338 37.0 33.0 37.0 22.0 37.0 84-85 32.06769749474882 35.0 33.0 37.0 22.0 37.0 86-87 31.929278180042296 33.0 33.0 37.0 22.0 37.0 88-89 31.82407163882343 33.0 33.0 37.0 22.0 37.0 90-91 31.694747579797163 33.0 33.0 37.0 18.5 37.0 92-93 31.518130713745357 33.0 33.0 37.0 15.0 37.0 94-95 31.36946622369481 33.0 33.0 37.0 15.0 37.0 96-97 31.227987947259273 33.0 33.0 37.0 15.0 37.0 98-99 31.07592088327352 33.0 33.0 37.0 15.0 37.0 100-101 29.776629593852547 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13211996997456055 2306 2 0.2067077801300421 2306 3 0.16800197883901546 2306 4 0.2691484198202403 2306 5 0.21764174762306965 2306 6 0.14536392704150813 2306 7 0.2893371195200345 2306 8 0.18088409296463226 2306 9 0.35546354445607875 2306 10-11 0.26186248790286726 2306 12-13 0.2286581289932741 2306 14-15 0.3914316039618626 2306 16-17 0.3987141886603425 2306 18-19 0.3662011982001019 2306 20-21 0.38408152852078103 2306 22-23 0.2979855377899838 2306 24-25 0.42017580583086556 2306 26-27 0.3146462894130124 2306 28-29 0.2690475669217278 2306 30-31 0.1607099839479389 2306 32-33 0.2919343431022625 2306 34-35 0.3399352955537651 2306 36-37 0.2707562018776031 2306 38-39 0.2253240696401022 2306 40-41 0.297370798567691 2306 42-43 0.35647409526040263 2306 44-45 0.4243691792869555 2306 46-47 0.37073461361954685 2306 48-49 0.40401554998106803 2306 50-51 0.46864613770527797 2306 52-53 0.3911028037504849 2306 54-55 0.426233176556039 2306 56-57 0.36549115560588774 2306 58-59 0.4260723418971395 2306 60-61 0.3218226394900583 2306 62-63 0.3915469835294374 2306 64-65 0.3676549405981788 2306 66-67 0.3828275560160037 2306 68-69 0.19231720656460993 2306 70-71 0.36083006376102844 2306 72-73 0.15507489789957418 2306 74-75 0.2381967501531861 2306 76-77 0.21802675922352677 2306 78-79 0.27272985326804644 2306 80-81 0.33486533520758854 2306 82-83 0.4404771939564185 2306 84-85 0.5262791592335461 2306 86-87 0.47013941441660023 2306 88-89 0.4226454216773625 2306 90-91 0.3701690919533256 2306 92-93 0.27650867701647996 2306 94-95 0.12477992904099722 2306 96-97 0.3012946954768694 2306 98-99 0.26971201239544484 2306 100-101 0.23119093607452612 2307 1 0.18982678617850723 2307 2 0.23700693512214954 2307 3 0.18085009374313188 2307 4 0.2745126766977677 2307 5 0.26610337221838876 2307 6 0.16530168325352435 2307 7 0.2735045605971749 2307 8 0.18794642270885475 2307 9 0.3357932737121061 2307 10-11 0.2457131183383794 2307 12-13 0.29863832828446135 2307 14-15 0.4638911057387958 2307 16-17 0.4817458883285397 2307 18-19 0.479450800084237 2307 20-21 0.41655123594015464 2307 22-23 0.4125066835496014 2307 24-25 0.4785523848046864 2307 26-27 0.27451621293294437 2307 28-29 0.2807770267549472 2307 30-31 0.35628641285853035 2307 32-33 0.38866515242747113 2307 34-35 0.37638630158097186 2307 36-37 0.3624039655151492 2307 38-39 0.3510798323625579 2307 40-41 0.34023808122376664 2307 42-43 0.31738977890311304 2307 44-45 0.3676027761051941 2307 46-47 0.38378447040694397 2307 48-49 0.23975336750248033 2307 50-51 0.13059935652866983 2307 52-53 0.11166064086488348 2307 54-55 0.29948881216257206 2307 56-57 0.1423632688767711 2307 58-59 0.27050457365474045 2307 60-61 0.30586929421517794 2307 62-63 0.35616432823976396 2307 64-65 0.3829847884024815 2307 66-67 0.389160027059269 2307 68-69 0.37103677051958783 2307 70-71 0.31744424606505106 2307 72-73 0.21673685515743557 2307 74-75 0.2808443862901342 2307 76-77 0.3504989635003142 2307 78-79 0.317912743877784 2307 80-81 0.3884172093088978 2307 82-83 0.4351435358218012 2307 84-85 0.48757456453331116 2307 86-87 0.46542881662767144 2307 88-89 0.27215958008289576 2307 90-91 0.33366856841757553 2307 92-93 0.4646131901527113 2307 94-95 0.46028432186647095 2307 96-97 0.2983758027079446 2307 98-99 0.3238674161019688 2307 100-101 0.35521622623279114 2201 1 0.25340821494270216 2201 2 0.263675644026673 2201 3 0.2676017891731668 2201 4 0.35113645795507153 2201 5 0.23998343545799372 2201 6 0.22706631312090764 2201 7 0.42491528237762566 2201 8 0.2873737557475877 2201 9 0.2974725196443231 2201 10-11 0.3468237754426795 2201 12-13 0.28813748372080283 2201 14-15 0.5732731749855589 2201 16-17 0.5200452369529742 2201 18-19 0.5220684828514379 2201 20-21 0.5003122262479209 2201 22-23 0.5018106689101742 2201 24-25 0.5991787651584772 2201 26-27 0.46033784977706915 2201 28-29 0.45947461493229014 2201 30-31 0.5285780653539973 2201 32-33 0.5312435126024369 2201 34-35 0.5576428233042492 2201 36-37 0.4685459324407262 2201 38-39 0.43660777066794765 2201 40-41 0.561616759848313 2201 42-43 0.5727472997532104 2201 44-45 0.6067489716364634 2201 46-47 0.5888678350952787 2201 48-49 0.5701838055989654 2201 50-51 0.4809495892216944 2201 52-53 0.4930051627441827 2201 54-55 0.6101307899494302 2201 56-57 0.6172376070323509 2201 58-59 0.6422879975013842 2201 60-61 0.6286752243792009 2201 62-63 0.6856272244406512 2201 64-65 0.6189604255196457 2201 66-67 0.6369421615054378 2201 68-69 0.5910220023492556 2201 70-71 0.5898897482026584 2201 72-73 0.5473955069918759 2201 74-75 0.5954997420034189 2201 76-77 0.4941745984135082 2201 78-79 0.6074886735042995 2201 80-81 0.6751686193330215 2201 82-83 0.6975977856506077 2201 84-85 0.7394317245827651 2201 86-87 0.5998384632206282 2201 88-89 0.520798090822165 2201 90-91 0.5157231643486107 2201 92-93 0.5295426386041981 2201 94-95 0.543925124573768 2201 96-97 0.49318333368201905 2201 98-99 0.586646806687412 2201 100-101 0.5366553126040685 1101 1 0.2269084024125192 1101 2 0.14943434266228195 1101 3 0.11820252019751365 1101 4 0.2273049058848784 1101 5 0.21930029687497665 1101 6 0.17892348774562095 1101 7 0.3378445109015331 1101 8 0.22400104055849823 1101 9 0.28228307437507283 1101 10-11 0.25810226203948616 1101 12-13 0.3007258841966376 1101 14-15 0.4398854118351494 1101 16-17 0.388109447345208 1101 18-19 0.43237824344420517 1101 20-21 0.40430184308264216 1101 22-23 0.3084848103811666 1101 24-25 0.38028461400095637 1101 26-27 0.364106676820505 1101 28-29 0.29136506634954173 1101 30-31 0.3108231981553118 1101 32-33 0.28167195850282667 1101 34-35 0.29504999695743805 1101 36-37 0.23925774922837917 1101 38-39 0.19097704087420908 1101 40-41 0.22112392652085333 1101 42-43 0.21147143989282569 1101 44-45 0.29020931187439913 1101 46-47 0.30685637388858567 1101 48-49 0.1549987502180059 1101 50-51 0.10589437378253308 1101 52-53 0.16110010235442473 1101 54-55 0.38099193490425876 1101 56-57 0.3215647415075864 1101 58-59 0.49619323492598255 1101 60-61 0.42923928904153996 1101 62-63 0.3901197353632 1101 64-65 0.35836456655164284 1101 66-67 0.4781789009842825 1101 68-69 0.38343685111041026 1101 70-71 0.441198834479799 1101 72-73 0.24751540131790506 1101 74-75 0.29971130007552205 1101 76-77 0.386381539186722 1101 78-79 0.3878295303385286 1101 80-81 0.5074346580777487 1101 82-83 0.39554878730076837 1101 84-85 0.49063214313341774 1101 86-87 0.5005403415890584 1101 88-89 0.43317613648708075 1101 90-91 0.22169449190120005 1101 92-93 0.3880137196753637 1101 94-95 0.4071367812384459 1101 96-97 0.28231468993440245 1101 98-99 0.34086624496882223 1101 100-101 0.2439802370224271 2304 1 0.13308577122733922 2304 2 0.1402501214090357 2304 3 0.14571121766404005 2304 4 0.19426356959203872 2304 5 0.16661813721194818 2304 6 0.14914261433860077 2304 7 0.22660973678065943 2304 8 0.11917556333393975 2304 9 0.2754729021807307 2304 10-11 0.26210162543058146 2304 12-13 0.2686922809758201 2304 14-15 0.3664444792161703 2304 16-17 0.3461038128071081 2304 18-19 0.35516504994687637 2304 20-21 0.3138117706114869 2304 22-23 0.13293601963076895 2304 24-25 0.2786022428760049 2304 26-27 0.22668114783551374 2304 28-29 0.17973123469231922 2304 30-31 0.27385294654440173 2304 32-33 0.2547774160118621 2304 34-35 0.14909070461859386 2304 36-37 0.25642979069374405 2304 38-39 0.20715455746389466 2304 40-41 0.21964194073522236 2304 42-43 0.2564311987033179 2304 44-45 0.27609615791011066 2304 46-47 0.25799367152037433 2304 48-49 0.24733630934593265 2304 50-51 0.25510309166291023 2304 52-53 0.3324155353995337 2304 54-55 0.4209560138411419 2304 56-57 0.38462694805840414 2304 58-59 0.3321062031124029 2304 60-61 0.3101174300912106 2304 62-63 0.32045233213252544 2304 64-65 0.3040975148682321 2304 66-67 0.4906928169961091 2304 68-69 0.36985503280732956 2304 70-71 0.3637233209719355 2304 72-73 0.31871996397817526 2304 74-75 0.41499587720465314 2304 76-77 0.4164663580389245 2304 78-79 0.45750942250733573 2304 80-81 0.40105343840674124 2304 82-83 0.375859943636506 2304 84-85 0.39772937782097273 2304 86-87 0.4221323948058 2304 88-89 0.3836835339270728 2304 90-91 0.31816348330632493 2304 92-93 0.44529014773966935 2304 94-95 0.3845629667100283 2304 96-97 0.30741229000512504 2304 98-99 0.32768658355112734 2304 100-101 0.3483134016134244 2202 1 0.13942455007386556 2202 2 0.09113278577655848 2202 3 0.08029334946698086 2202 4 0.15876842013940973 2202 5 0.16330745710306616 2202 6 0.18007023062201455 2202 7 0.2137009059583903 2202 8 0.20372782615960716 2202 9 0.20442015836077587 2202 10-11 0.17866765065686963 2202 12-13 0.19843272093195452 2202 14-15 0.2693567682560669 2202 16-17 0.3070374646523959 2202 18-19 0.28282616080984724 2202 20-21 0.2976557106390132 2202 22-23 0.2423822787627543 2202 24-25 0.18778953225456263 2202 26-27 0.2489747661640962 2202 28-29 0.23050640209277162 2202 30-31 0.26700995250235593 2202 32-33 0.15038789647248763 2202 34-35 0.2089210163534787 2202 36-37 0.26948677903657625 2202 38-39 0.15115103808815888 2202 40-41 0.17290152433924533 2202 42-43 0.17797710644286013 2202 44-45 0.18600118946584843 2202 46-47 0.21744173568239944 2202 48-49 0.28882277813654156 2202 50-51 0.1884576158695097 2202 52-53 0.18573066432838914 2202 54-55 0.3765417231598107 2202 56-57 0.38488217907171673 2202 58-59 0.35102984322932684 2202 60-61 0.33684751511760425 2202 62-63 0.3848885938781166 2202 64-65 0.45702570759144123 2202 66-67 0.5200275615361605 2202 68-69 0.5051971325581732 2202 70-71 0.5064919085641222 2202 72-73 0.362748436800409 2202 74-75 0.43538808414481167 2202 76-77 0.44609968706810577 2202 78-79 0.4330487620900101 2202 80-81 0.3760292412947379 2202 82-83 0.44062127456341926 2202 84-85 0.4570038034745707 2202 86-87 0.4664401150163613 2202 88-89 0.3930340457495909 2202 90-91 0.3473998695018956 2202 92-93 0.3495934797268525 2202 94-95 0.35189595890867764 2202 96-97 0.4270395970423273 2202 98-99 0.4007682606806924 2202 100-101 0.48868676230866015 1102 1 0.0663959163586405 1102 2 0.01798014844735718 1102 3 0.07686857516115353 1102 4 0.0995038288787029 1102 5 0.0717321702194269 1102 6 0.1206218599708464 1102 7 0.12663336249593726 1102 8 0.18416407749891306 1102 9 0.17273578965247083 1102 10-11 0.21195693460932574 1102 12-13 0.21293726140118707 1102 14-15 0.18334642048469618 1102 16-17 0.1902825655918008 1102 18-19 0.21715951636832642 1102 20-21 0.1894578627357646 1102 22-23 0.1663498331549178 1102 24-25 0.1561429638652072 1102 26-27 0.14030215131885626 1102 28-29 0.11694001215721528 1102 30-31 0.14425349585341252 1102 32-33 0.07531323477638097 1102 34-35 0.07953848978565503 1102 36-37 0.19360946982004634 1102 38-39 0.1557305630806738 1102 40-41 0.10269594039974805 1102 42-43 0.12309595959195008 1102 44-45 0.23501716793391125 1102 46-47 0.1492525239335194 1102 48-49 0.20255891608545085 1102 50-51 0.11943891763526437 1102 52-53 0.23958344838570866 1102 54-55 0.2803596714222252 1102 56-57 0.38845841840100803 1102 58-59 0.37807135584975526 1102 60-61 0.376346928331742 1102 62-63 0.4453950253127914 1102 64-65 0.3295838998267939 1102 66-67 0.4058733623986299 1102 68-69 0.31534935084504667 1102 70-71 0.268710315705313 1102 72-73 0.30819447423400703 1102 74-75 0.37595961061938254 1102 76-77 0.36323905641150844 1102 78-79 0.30180024171455244 1102 80-81 0.32966153228314 1102 82-83 0.2572630575146846 1102 84-85 0.34550119701439286 1102 86-87 0.3238252929869745 1102 88-89 0.283578775458853 1102 90-91 0.23897594762860663 1102 92-93 0.3043746134536818 1102 94-95 0.24937654602369008 1102 96-97 0.32511778367356925 1102 98-99 0.398679611073085 1102 100-101 0.24965580719176472 2305 1 0.10427711900284464 2305 2 -0.004197638719773522 2305 3 -6.391555649400971E-4 2305 4 -0.00996376465332105 2305 5 -0.0361132698753579 2305 6 0.09920906669537288 2305 7 0.05172658065886537 2305 8 0.2100167330644922 2305 9 -0.04821209806624438 2305 10-11 0.032975501626843595 2305 12-13 0.08897682739577562 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0.06612790198553853 1213 88-89 -0.005150612018159251 1213 90-91 -0.03391208519775191 1213 92-93 -0.045956954250673476 1213 94-95 0.11906121732470965 1213 96-97 0.11163876887404456 1213 98-99 -0.041308043184663745 1213 100-101 0.138688459374805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3840.0 3 898.0 4 867.0 5 837.0 6 2314.0 7 7048.0 8 29006.0 9 68197.0 10 81693.0 11 53133.0 12 30713.0 13 21654.0 14 19498.0 15 19957.0 16 21220.0 17 21377.0 18 22394.0 19 24256.0 20 26804.0 21 29281.0 22 32865.0 23 35854.0 24 40015.0 25 46714.0 26 54900.0 27 65704.0 28 80492.0 29 100186.0 30 122328.0 31 158349.0 32 216774.0 33 310260.0 34 473763.0 35 805883.0 36 1563825.0 37 2516844.0 38 699317.0 39 616.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.39364727096193 26.236665613529475 22.362687606116417 15.006999509392186 2 32.646507571056375 24.250941847913246 29.81494827973843 13.287602301291951 3 36.520679178901894 20.41372221112587 25.947865230154033 17.117733379818205 4 30.869588431499285 19.240777352976963 31.211479787107884 18.678154428415873 5 30.866642783971006 17.815652373031618 32.86421506645546 18.45348977654191 6 27.428474882597126 18.180209915286223 36.524359220080065 17.866955982036583 7 33.54486904911524 18.835330961657334 28.300344465847093 19.31945552338033 8 29.891888335782106 27.75452113174393 23.668725331892922 18.68486520058104 9 29.212084301626735 27.388104816091037 24.14674252744611 19.253068354836124 10-11 25.020427962939173 28.61246122404713 28.708550208176774 17.65856060483692 12-13 25.72379198960983 25.842020072424237 28.55896437302733 19.875223564938608 14-15 24.67503008826318 26.54142563358458 28.877462230350048 19.90608204780219 16-17 24.401837711081267 27.545824334766372 29.597654670051902 18.454683284100458 18-19 23.79632168753613 27.397616431369997 30.019789355413927 18.786272525679944 20-21 23.116885830951876 28.576420543109123 29.52174301298652 18.78495061295249 22-23 24.430257366173958 27.615719489062545 28.85215800901708 19.101865135746415 24-25 23.197676833204287 28.29081438006764 29.192715635820427 19.31879315090765 26-27 22.107397685581905 29.26914065141954 29.24476930447461 19.378692358523946 28-29 22.398486197884708 29.25040762946618 28.654986666601662 19.696119506047452 30-31 22.763370107329465 29.03217251059504 28.77923368938576 19.425223692689737 32-33 22.769267691474372 28.584498328453144 29.27215090263273 19.374083077439753 34-35 22.045572242694167 28.459430825902544 29.00510974513462 20.48988718626867 36-37 20.575759002614028 29.122044188755403 29.40957230962873 20.89262449900184 38-39 20.63301048863041 29.33021062960435 29.327531713674897 20.709247168090346 40-41 21.325615625973914 28.28912967766941 29.98237695784186 20.40287773851481 42-43 22.187767409065014 28.266616772796088 29.9951708147586 19.5504450033803 44-45 21.213132690579652 28.827832770981676 30.067113464499894 19.89192107393877 46-47 22.12639241445304 29.180812561123787 29.07309398026935 19.61970104415382 48-49 21.52004575616339 29.06232118467446 29.12752191851039 20.29011114065176 50-51 20.768229446940673 28.67662682905845 29.195553864108355 21.359589859892527 52-53 22.650963799653535 28.385977276370184 28.2531534810274 20.709905442948887 54-55 23.61331003441776 28.113372410108024 28.274225889418886 19.999091666055328 56-57 23.885591172152633 27.629012906108912 28.121783783936582 20.363612137801873 58-59 23.00100340721631 27.675030963013974 28.569784980497154 20.75418064927256 60-61 21.4116979582945 27.852237614280124 28.252102435680985 22.483961991744394 62-63 22.05308531810247 27.98006908548044 28.48103899466447 21.485806601752618 64-65 21.548546085801863 28.62158143421664 29.242230106839223 20.587642373142273 66-67 21.53372946899374 28.219899740525744 28.737807677652764 21.50856311282775 68-69 21.164984257012893 27.495948248040758 30.510785672195844 20.8282818227505 70-71 20.919877704432 29.40183073490996 29.541389051523563 20.136902509134476 72-73 21.031399478039823 31.065825477551183 28.047349474139082 19.855425570269908 74-75 21.85310749988411 30.6907418652691 27.56677354988882 19.889377084957975 76-77 21.944554967310665 29.87089466763857 27.769206682239954 20.41534368281081 78-79 21.65058255205109 29.832580175713375 28.30604260661542 20.210794665620107 80-81 21.061902871163152 30.570132353839274 28.096114845241697 20.271849929755874 82-83 20.44868017522041 30.430594945976193 28.394432966143313 20.726291912660084 84-85 20.61413872312248 29.97004162188217 28.55942558527059 20.85639406972476 86-87 20.479273558963122 30.18583965360714 29.04433349465192 20.29055329277782 88-89 20.530189603931458 30.541571363697965 28.893746904861462 20.03449212750911 90-91 21.047769938095513 29.58429374008336 28.67277867295851 20.69515764886262 92-93 20.739829267600413 30.020765862047842 28.163178655123712 21.076226215228033 94-95 20.44902903270051 30.561005040923796 27.75554961377575 21.234416312599947 96-97 20.7464637716622 30.90866353063126 27.458587859246407 20.88628483846014 98-99 21.02869686933843 30.00024599647165 27.78529505028765 21.18576208390227 100-101 21.35502687295967 30.09034771383622 27.296051905617137 21.258573507586974 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4509.0 1 3220.5 2 1570.0 3 1539.5 4 1771.5 5 1724.5 6 2081.0 7 2663.5 8 3134.5 9 3448.0 10 3595.5 11 3699.5 12 3585.5 13 3239.0 14 2920.5 15 2596.5 16 2406.0 17 2433.5 18 2589.0 19 2927.0 20 3486.5 21 4392.0 22 5733.0 23 7609.5 24 10147.0 25 13598.5 26 18009.0 27 23382.5 28 30020.5 29 37550.0 30 46208.0 31 56865.5 32 72531.5 33 95400.5 34 127016.5 35 178951.5 36 249689.0 37 332296.0 38 414443.0 39 454250.0 40 471837.5 41 460343.5 42 433378.5 43 447887.5 44 449699.0 45 440068.0 46 456754.5 47 427209.5 48 343585.5 49 276681.5 50 232746.5 51 208107.5 52 190257.5 53 163695.0 54 130465.0 55 98188.5 56 75618.0 57 62476.0 58 52392.0 59 40664.5 60 29866.0 61 22245.0 62 16318.5 63 11820.5 64 8782.5 65 6251.5 66 4469.0 67 3492.5 68 2582.0 69 1690.5 70 1160.0 71 872.0 72 655.5 73 489.0 74 372.0 75 285.5 76 238.5 77 210.5 78 168.0 79 124.0 80 103.5 81 93.5 82 75.0 83 59.0 84 45.0 85 36.5 86 28.5 87 19.0 88 15.0 89 14.0 90 13.5 91 11.5 92 8.5 93 7.0 94 5.5 95 4.5 96 4.5 97 2.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29983318129971076 2 0.2756708472925125 3 0.24748785993170525 4 0.2344783573607919 5 0.2409703040177339 6 0.18850974099309625 7 0.15488478651355064 8 0.15243910246724704 9 0.13190047832970278 10-11 0.1164452917124859 12-13 0.1378738375318003 14-15 0.14026190074978784 16-17 0.13467268040313068 18-19 0.12193847734528296 20-21 0.12816792911767402 22-23 0.15828441538419774 24-25 0.5686983685366717 26-27 0.22714130522188117 28-29 0.19881106463315507 30-31 0.302234049146213 32-33 0.3350587143435912 34-35 0.3431960557646693 36-37 0.3340663556337036 38-39 0.3415890748860772 40-41 0.3453088194695913 42-43 0.3562887884209281 44-45 0.33663368365089663 46-47 0.3562823861066708 48-49 0.3528315387219649 50-51 0.35907379512287063 52-53 0.3432728835357574 54-55 0.3354172439420022 56-57 0.3438042756191166 58-59 0.3354300485705169 60-61 0.3126890283284479 62-63 0.29937221467318237 64-65 0.3097759753413586 66-67 0.326185106782919 68-69 0.31324602966883647 70-71 0.29408390309662014 72-73 0.2904537909127088 74-75 0.2823292541201453 76-77 0.28423074145457505 78-79 0.29327721150019537 80-81 0.3023877046883891 82-83 0.32553207072867046 84-85 0.3163831636549327 86-87 0.31849592735985466 88-89 0.32384185976473284 90-91 0.3172986945937322 92-93 0.2988472249040805 94-95 0.29460889286572195 96-97 0.312029589959942 98-99 0.32026296609488025 100-101 0.3212041062907091 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7809676.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.49164550283676 #Duplication Level Percentage of deduplicated Percentage of total 1 89.83587590472023 40.867818200943674 2 5.119746735721555 4.658114071315014 3 1.4473727632110733 1.9753010596337832 4 0.7505492313006815 1.3657487825102894 5 0.4542546439060093 1.0332395614294758 6 0.33396530560481713 0.9115578775692532 7 0.2619716181582966 0.834226398954322 8 0.19571910751689697 0.7122867405832216 9 0.14143478850427357 0.5790691134364595 >10 1.1193474050773276 10.308879180165844 >50 0.15753375620177423 4.977752701633706 >100 0.14945482739018 14.25539553340616 >500 0.020070456634373533 6.323900340025511 >1k 0.012334864881239789 9.810335081311726 >5k 1.9861718419734005E-4 0.5068284486911963 >10k+ 1.6997398704504907E-4 0.8795469083903616 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 14875 0.19046884915584206 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 11362 0.14548618918377665 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 11230 0.14379597821983908 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 10365 0.1327199745546422 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10333 0.1323102264421725 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10084 0.12912187394201757 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00224080999006873 0.0 0.0 2 0.0024584886748182638 0.0 0.0 3 0.0028426275302586177 0.0 0.0 4 0.002970673815405402 0.0 0.0 5 0.003431640441933827 0.0 1.2804628514678458E-5 6 0.004097481124697107 0.0 1.2804628514678458E-5 7 0.004852954207063135 0.0 2.5609257029356916E-5 8 0.005531599518341094 0.0 2.5609257029356916E-5 9 0.0062102448296190525 0.0 5.121851405871383E-5 10-11 0.010557416210352388 0.0 6.40231425733923E-5 12-13 0.014328379307925194 0.0 6.40231425733923E-5 14-15 0.019232552029047043 1.2804628514678458E-5 6.40231425733923E-5 16-17 0.02615345374123075 1.2804628514678458E-5 7.042545683073152E-5 18-19 0.033791414650236454 1.2804628514678458E-5 7.682777108807074E-5 20-21 0.038919668370365174 1.2804628514678458E-5 7.682777108807074E-5 22-23 0.04391987580534711 1.2804628514678458E-5 7.682777108807074E-5 24-25 0.04989323500744461 1.2804628514678458E-5 7.682777108807074E-5 26-27 0.05587299652379945 1.2804628514678458E-5 7.682777108807074E-5 28-29 0.06322925560548223 1.2804628514678458E-5 7.682777108807074E-5 30-31 0.11150910742007736 1.2804628514678458E-5 7.682777108807074E-5 32-33 0.12226499537240726 1.2804628514678458E-5 7.682777108807074E-5 34-35 0.1304983715073455 1.2804628514678458E-5 7.682777108807074E-5 36-37 0.13951282998167913 1.2804628514678458E-5 7.682777108807074E-5 38-39 0.15013426933460491 1.2804628514678458E-5 7.682777108807074E-5 40-41 0.16146636557009536 1.2804628514678458E-5 7.682777108807074E-5 42-43 0.17408532697131096 1.2804628514678458E-5 7.682777108807074E-5 44-45 0.18763902625409812 1.2804628514678458E-5 7.682777108807074E-5 46-47 0.201640887534899 1.2804628514678458E-5 7.682777108807074E-5 48-49 0.2172753389513214 1.2804628514678458E-5 7.682777108807074E-5 50-51 0.23459359901742402 1.2804628514678458E-5 7.682777108807074E-5 52-53 0.25351883996211877 1.2804628514678458E-5 7.682777108807074E-5 54-55 0.27371814144402407 1.2804628514678458E-5 7.682777108807074E-5 56-57 0.2950890664350224 1.2804628514678458E-5 7.682777108807074E-5 58-59 0.31756118947828305 1.2804628514678458E-5 7.682777108807074E-5 60-61 0.3424405826823034 1.2804628514678458E-5 8.323008534540997E-5 62-63 0.36995132704609 1.2804628514678458E-5 8.96323996027492E-5 64-65 0.400752860938149 1.2804628514678458E-5 8.96323996027492E-5 66-67 0.43367356084938735 1.2804628514678458E-5 1.0243702811742767E-4 68-69 0.4703332122869118 1.2804628514678458E-5 1.0243702811742767E-4 70-71 0.5091248343721302 1.2804628514678458E-5 1.1524165663210613E-4 72-73 0.5525261227226328 1.2804628514678458E-5 1.1524165663210613E-4 74-75 0.6003578125392142 1.2804628514678458E-5 1.1524165663210613E-4 76-77 0.6528952033349399 1.2804628514678458E-5 1.1524165663210613E-4 78-79 0.7113483325044471 1.2804628514678458E-5 1.1524165663210613E-4 80-81 0.7773177786120704 1.2804628514678458E-5 1.344485994041238E-4 82-83 0.8499904477471281 1.2804628514678458E-5 1.4085091366146304E-4 84-85 0.932068116526217 1.2804628514678458E-5 1.4085091366146304E-4 86-87 1.0203816393919543 1.2804628514678458E-5 1.4085091366146304E-4 88-89 1.1184714961286486 1.2804628514678458E-5 1.4725322791880226E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2585 0.0 75.75294 9 ATCGGGA 4180 0.0 72.91609 2 AAGCGGC 1210 0.0 68.18265 9 CGGAAAG 2290 0.0 67.76956 5 CGGGAGA 3705 0.0 67.763 4 AGAGCGG 3160 0.0 65.27438 8 ATTCGGA 825 0.0 60.99813 2 TCGGAGA 1130 0.0 57.95966 4 GTTCGGA 805 0.0 57.7956 2 GGATCGG 1975 0.0 55.537304 1 TCGGAAA 1910 0.0 55.41109 4 TCGGGAG 4800 0.0 52.40647 3 TCGGGAA 1775 0.0 52.14186 3 GGTCCGG 645 0.0 50.79597 1 GGTTCGG 1135 0.0 50.20249 1 GATCCGG 1250 0.0 49.76239 1 CGGAGAG 2300 0.0 48.284798 5 GAACCGG 645 0.0 47.851276 1 GATCGGG 6860 0.0 47.760063 1 GAGAGCG 4735 0.0 47.467785 7 >>END_MODULE