FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_40255-1.342000000357f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_40255-1.342000000357f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7809676
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA148750.19046884915584206No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC113620.14548618918377665No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG112300.14379597821983908No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC103650.1327199745546422No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103330.1323102264421725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100840.12912187394201757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC25850.075.752949
ATCGGGA41800.072.916092
AAGCGGC12100.068.182659
CGGAAAG22900.067.769565
CGGGAGA37050.067.7634
AGAGCGG31600.065.274388
ATTCGGA8250.060.998132
TCGGAGA11300.057.959664
GTTCGGA8050.057.79562
GGATCGG19750.055.5373041
TCGGAAA19100.055.411094
TCGGGAG48000.052.406473
TCGGGAA17750.052.141863
GGTCCGG6450.050.795971
GGTTCGG11350.050.202491
GATCCGG12500.049.762391
CGGAGAG23000.048.2847985
GAACCGG6450.047.8512761
GATCGGG68600.047.7600631
GAGAGCG47350.047.4677857
Produced by FastQC (version 0.11.2)