##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_35011-1.342000000358a4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6163373 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.457618904453778 33.0 33.0 33.0 27.0 33.0 2 31.219021629877016 33.0 33.0 33.0 27.0 33.0 3 31.118668625118097 33.0 33.0 33.0 27.0 33.0 4 34.688693350215864 37.0 37.0 37.0 33.0 37.0 5 34.72313520534941 37.0 37.0 37.0 33.0 37.0 6 34.954615110914105 37.0 37.0 37.0 33.0 37.0 7 34.752846371621516 37.0 37.0 37.0 33.0 37.0 8 34.78621251707466 37.0 37.0 37.0 33.0 37.0 9 34.51374401646631 37.0 37.0 37.0 33.0 37.0 10-11 34.513255485267564 37.0 37.0 37.0 33.0 37.0 12-13 34.493708477484645 37.0 37.0 37.0 33.0 37.0 14-15 36.193855296442386 40.0 37.0 40.0 33.0 40.0 16-17 36.15897488274683 40.0 37.0 40.0 30.0 40.0 18-19 36.175106796229926 40.0 37.0 40.0 27.0 40.0 20-21 36.16593422140766 40.0 37.0 40.0 27.0 40.0 22-23 36.47738292003421 40.0 37.0 40.0 27.0 40.0 24-25 36.0697592049029 40.0 37.0 40.0 27.0 40.0 26-27 35.94153947846414 40.0 37.0 40.0 27.0 40.0 28-29 35.83608472179114 40.0 37.0 40.0 27.0 40.0 30-31 35.83037202194318 40.0 37.0 40.0 27.0 40.0 32-33 35.67253855964908 40.0 37.0 40.0 27.0 40.0 34-35 35.70799236067653 40.0 37.0 40.0 27.0 40.0 36-37 35.60249971890392 40.0 37.0 40.0 27.0 40.0 38-39 35.505509888822246 40.0 37.0 40.0 27.0 40.0 40-41 35.39123390714792 40.0 37.0 40.0 27.0 40.0 42-43 35.301953329775756 37.0 37.0 40.0 27.0 40.0 44-45 35.194391204296736 37.0 37.0 40.0 22.0 40.0 46-47 35.09661349394236 37.0 37.0 40.0 22.0 40.0 48-49 34.99251684102195 37.0 37.0 40.0 22.0 40.0 50-51 33.95881200115586 37.0 35.0 38.5 22.0 38.5 52-53 34.36123247773581 37.0 35.0 38.5 22.0 40.0 54-55 35.08166210287776 37.0 37.0 40.0 24.5 40.0 56-57 35.266780462581124 37.0 37.0 40.0 24.5 40.0 58-59 34.96887548425188 37.0 37.0 40.0 22.0 40.0 60-61 34.737015186327355 37.0 37.0 40.0 22.0 40.0 62-63 34.45814556412536 37.0 33.0 40.0 22.0 40.0 64-65 34.243962031829 37.0 33.0 40.0 22.0 40.0 66-67 33.99522144773649 37.0 33.0 38.5 22.0 40.0 68-69 33.90130834203933 37.0 33.0 37.0 22.0 40.0 70-71 33.59208188438376 37.0 33.0 37.0 22.0 40.0 72-73 33.537393404552994 37.0 33.0 37.0 22.0 40.0 74-75 33.4549762767887 37.0 33.0 37.0 27.0 38.5 76-77 33.21216750957633 37.0 33.0 37.0 22.0 37.0 78-79 32.89618695801796 37.0 33.0 37.0 22.0 37.0 80-81 32.5263254390088 37.0 33.0 37.0 22.0 37.0 82-83 32.25046837178279 37.0 33.0 37.0 22.0 37.0 84-85 31.981583704247658 37.0 33.0 37.0 15.0 37.0 86-87 31.826340706622815 37.0 33.0 37.0 15.0 37.0 88-89 31.698437852130645 33.0 33.0 37.0 15.0 37.0 90-91 31.559261706211842 33.0 33.0 37.0 15.0 37.0 92-93 31.406758685544425 33.0 33.0 37.0 15.0 37.0 94-95 31.26989912828576 33.0 33.0 37.0 15.0 37.0 96-97 31.12233252798427 33.0 33.0 37.0 15.0 37.0 98-99 30.985497551421926 33.0 33.0 37.0 15.0 37.0 100-101 29.7369855759176 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14407040162582518 2306 2 0.2264976685079212 2306 3 0.20738148076452845 2306 4 0.34314427311049656 2306 5 0.30834041956924096 2306 6 0.13120535595138705 2306 7 0.3969176424329177 2306 8 0.2836773454158532 2306 9 0.42215333119780496 2306 10-11 0.35707387535155277 2306 12-13 0.36892759200662084 2306 14-15 0.5518243671779288 2306 16-17 0.521725479275787 2306 18-19 0.39744895340093933 2306 20-21 0.5134679575858456 2306 22-23 0.34453932611142335 2306 24-25 0.47945756735405354 2306 26-27 0.3078831661399306 2306 28-29 0.30674624861100597 2306 30-31 0.34267945038228476 2306 32-33 0.39459611912715786 2306 34-35 0.37962898185668337 2306 36-37 0.44399202570935614 2306 38-39 0.2656296946450212 2306 40-41 0.36852105198288143 2306 42-43 0.3670988356903493 2306 44-45 0.4221782200449624 2306 46-47 0.4386744375513061 2306 48-49 0.4256513523872698 2306 50-51 0.2771267548840868 2306 52-53 0.2765494324673554 2306 54-55 0.377486042738866 2306 56-57 0.2815173617139024 2306 58-59 0.33246986629014685 2306 60-61 0.40925690855311814 2306 62-63 0.4054603044659686 2306 64-65 0.3091478137312009 2306 66-67 0.3717253448995024 2306 68-69 0.18431593695092374 2306 70-71 0.24866696651022124 2306 72-73 0.11946159840149306 2306 74-75 0.050927545893806325 2306 76-77 0.058951975676947654 2306 78-79 0.23971385230267828 2306 80-81 0.3320332894264837 2306 82-83 0.43696270805660475 2306 84-85 0.4772764485384329 2306 86-87 0.4884196539967185 2306 88-89 0.4200703711168501 2306 90-91 0.30235209318653844 2306 92-93 0.20571042165463638 2306 94-95 0.1811909674386989 2306 96-97 0.18705735900841702 2306 98-99 0.3270616561309261 2306 100-101 0.3368029766690057 2307 1 0.15785546873678769 2307 2 0.21544243171437216 2307 3 0.1357632898605381 2307 4 0.2638570743182953 2307 5 0.23253086518171528 2307 6 0.10760134448320002 2307 7 0.31660113670490375 2307 8 0.1929560093077214 2307 9 0.3297948308244827 2307 10-11 0.21813427749242464 2307 12-13 0.2615910243854884 2307 14-15 0.4396700215833036 2307 16-17 0.3809557598424078 2307 18-19 0.38866266044901465 2307 20-21 0.28787596558359496 2307 22-23 0.3145374606406648 2307 24-25 0.400205694520821 2307 26-27 0.1266309661060987 2307 28-29 0.18026247704533205 2307 30-31 0.17528812543446293 2307 32-33 0.2918928494715871 2307 34-35 0.14193667602241788 2307 36-37 0.24909987137955625 2307 38-39 0.1532245835152679 2307 40-41 0.16493940851243138 2307 42-43 0.15066509274311812 2307 44-45 0.23925218270897375 2307 46-47 0.190635550847162 2307 48-49 0.14954539903649078 2307 50-51 0.10500790618798561 2307 52-53 0.048268138614737666 2307 54-55 0.1091644814736199 2307 56-57 -0.013185119390072941 2307 58-59 0.22146998975681953 2307 60-61 0.10789712158096165 2307 62-63 0.2267595862690186 2307 64-65 0.19953737846615383 2307 66-67 0.21441527290887308 2307 68-69 0.10686977570977518 2307 70-71 0.066547039222705 2307 72-73 -0.044973424548537366 2307 74-75 0.06385141211168133 2307 76-77 0.08343922902115963 2307 78-79 0.14606080998278514 2307 80-81 0.24118700022221162 2307 82-83 0.23357014323962488 2307 84-85 0.24930902385705167 2307 86-87 0.26490364852702797 2307 88-89 0.14111639296793754 2307 90-91 0.07592697989960229 2307 92-93 0.16701741803396786 2307 94-95 0.16014099261047576 2307 96-97 0.2556248827790064 2307 98-99 0.1621418012325968 2307 100-101 0.08417283012271426 2201 1 0.11652279870416749 2201 2 0.08636222294293106 2201 3 0.04213665955373003 2201 4 0.10284069671947549 2201 5 0.10878955401329193 2201 6 0.08401352192830558 2201 7 0.23946045468834143 2201 8 0.028957626394472413 2201 9 0.1135998338775579 2201 10-11 0.033751954275210494 2201 12-13 0.14713974197174906 2201 14-15 0.3324209370198403 2201 16-17 0.21965821730422874 2201 18-19 0.2937730532864009 2201 20-21 0.21068745187038473 2201 22-23 0.22009231932485562 2201 24-25 0.28721609438733253 2201 26-27 0.08660314993566942 2201 28-29 0.07199630549821734 2201 30-31 0.10974213147174083 2201 32-33 0.1271298713860176 2201 34-35 0.05057133933581781 2201 36-37 0.09802238959734666 2201 38-39 0.03979653695011365 2201 40-41 0.09399380499615972 2201 42-43 0.10548161514446264 2201 44-45 0.12043578872181371 2201 46-47 0.09863325647912546 2201 48-49 0.20330835550394966 2201 50-51 0.060643586847270115 2201 52-53 0.12286150770469106 2201 54-55 0.283690238939343 2201 56-57 0.18133195717287265 2201 58-59 0.2651717850829769 2201 60-61 0.196876180877112 2201 62-63 0.23712670198405306 2201 64-65 0.26308091365959996 2201 66-67 0.10219868218391781 2201 68-69 0.12343287607362186 2201 70-71 0.171021885381343 2201 72-73 0.10054638948907524 2201 74-75 0.1324619356286334 2201 76-77 0.19710708131843546 2201 78-79 0.264926200714676 2201 80-81 0.2878700241640786 2201 82-83 0.2456593942081966 2201 84-85 0.2734922507114561 2201 86-87 0.2409867646112538 2201 88-89 0.1134949150215867 2201 90-91 0.14879905515963898 2201 92-93 0.2377135718089285 2201 94-95 0.2538131245177375 2201 96-97 0.22693531463255923 2201 98-99 0.1842422457243309 2201 100-101 0.08777418794743141 1101 1 0.01808344957682806 1101 2 -0.026218223861675938 1101 3 -0.08816815223471863 1101 4 -0.04088583353892261 1101 5 -0.03838556911905755 1101 6 -0.05426228243893405 1101 7 0.26244752553959216 1101 8 0.025132066074242232 1101 9 0.11188555737965089 1101 10-11 0.06488858229353411 1101 12-13 0.07190687415228325 1101 14-15 0.22190054533498227 1101 16-17 0.10119114701748799 1101 18-19 0.16013394044414753 1101 20-21 0.15713071623348185 1101 22-23 0.18220098223758185 1101 24-25 0.2690647832268951 1101 26-27 0.11072971164498568 1101 28-29 0.12266800431135039 1101 30-31 0.21006256791367406 1101 32-33 0.11988843924036274 1101 34-35 0.07655342441054813 1101 36-37 0.11456125667892536 1101 38-39 0.021568292011998835 1101 40-41 0.10453925660912944 1101 42-43 0.08284114950962618 1101 44-45 0.17954310690628716 1101 46-47 0.1107185731354079 1101 48-49 0.031369749303685524 1101 50-51 0.015116693290472938 1101 52-53 0.10940735043801197 1101 54-55 0.23699325036449181 1101 56-57 0.23728567594120875 1101 58-59 0.323321846271142 1101 60-61 0.21640641306954933 1101 62-63 0.229971396960579 1101 64-65 0.26932968636827326 1101 66-67 0.27775049454292144 1101 68-69 0.16805170509152845 1101 70-71 0.2928595865613559 1101 72-73 -0.03748020669528529 1101 74-75 0.02294559425717324 1101 76-77 0.16969361669768546 1101 78-79 0.18829685176877575 1101 80-81 0.26036982759585925 1101 82-83 0.292556398508502 1101 84-85 0.31555317274745676 1101 86-87 0.29758942060073323 1101 88-89 0.2665579720908866 1101 90-91 0.18738071967917236 1101 92-93 0.23426937399879577 1101 94-95 0.34631816976875385 1101 96-97 0.30962749283022006 1101 98-99 0.19329194421892382 1101 100-101 0.19155180578251674 2304 1 0.10203754737206694 2304 2 0.06057885645218519 2304 3 0.08710194122672732 2304 4 0.15071628237127044 2304 5 0.048981502309779046 2304 6 0.13946091721916076 2304 7 0.2820993975802608 2304 8 0.17187223725152734 2304 9 0.15203798988909512 2304 10-11 0.1502076371070089 2304 12-13 0.18070897056791324 2304 14-15 0.2607883803509665 2304 16-17 0.27307912074481067 2304 18-19 0.24429449319006835 2304 20-21 0.1819033608587901 2304 22-23 0.13975388936471944 2304 24-25 0.1201890948250437 2304 26-27 0.002238741003054656 2304 28-29 0.023226711071842487 2304 30-31 0.1403161097388974 2304 32-33 0.11120203557538133 2304 34-35 0.04119271870726493 2304 36-37 0.19482447080798693 2304 38-39 0.05901270438204875 2304 40-41 0.04272007620566143 2304 42-43 0.11169037028933815 2304 44-45 0.08913975684011888 2304 46-47 0.039786657906773826 2304 48-49 0.1517145406930851 2304 50-51 0.16986152066984062 2304 52-53 0.14324151916348882 2304 54-55 0.27372202471864426 2304 56-57 0.33440250462520993 2304 58-59 0.1954059278334057 2304 60-61 0.21729487790997837 2304 62-63 0.19592961110838303 2304 64-65 0.2537358706443982 2304 66-67 0.29736960071706875 2304 68-69 0.1783085459971261 2304 70-71 0.2415737324931726 2304 72-73 0.20830787987558352 2304 74-75 0.3480647547911744 2304 76-77 0.2541604965549311 2304 78-79 0.31231884313869074 2304 80-81 0.32009499170636246 2304 82-83 0.307290428897538 2304 84-85 0.32877262532505824 2304 86-87 0.1826849252288234 2304 88-89 0.15009102475245584 2304 90-91 0.16608822996068184 2304 92-93 0.16265837306530884 2304 94-95 0.16013992945324063 2304 96-97 0.09377155017423533 2304 98-99 0.2426741164384154 2304 100-101 0.2075062709753439 2202 1 0.08792400974355274 2202 2 -0.005035152896166295 2202 3 0.09051594632808602 2202 4 0.07032665915972558 2202 5 0.03649843858251245 2202 6 0.12932976503966387 2202 7 0.1656830508401228 2202 8 0.1365983212212427 2202 9 0.08229363970281867 2202 10-11 0.10101057431793947 2202 12-13 0.06091295320155865 2202 14-15 0.14766972895399988 2202 16-17 0.145258557400183 2202 18-19 0.14629714452857456 2202 20-21 0.13915418150790515 2202 22-23 0.21031016715460993 2202 24-25 0.15801396583634642 2202 26-27 0.20642627838833505 2202 28-29 0.1836273475144239 2202 30-31 0.20282543451352097 2202 32-33 0.06044330572482437 2202 34-35 0.18095947317273442 2202 36-37 0.20990764710820997 2202 38-39 0.21019012178418706 2202 40-41 0.22118323200448486 2202 42-43 0.256634425778266 2202 44-45 0.2683151461641202 2202 46-47 0.25128685246880167 2202 48-49 0.2046635411737654 2202 50-51 0.14179867175780458 2202 52-53 0.24430477098852066 2202 54-55 0.35575346323938817 2202 56-57 0.3772362791588435 2202 58-59 0.32540941919449295 2202 60-61 0.3391454826793492 2202 62-63 0.4158581954000766 2202 64-65 0.3879763948118793 2202 66-67 0.4795248846549143 2202 68-69 0.44231118602868946 2202 70-71 0.45601052437910283 2202 72-73 0.3001893829148017 2202 74-75 0.4477080209732591 2202 76-77 0.42208117591974315 2202 78-79 0.42949737386032183 2202 80-81 0.3740163197031947 2202 82-83 0.4233687051579622 2202 84-85 0.42078281370135784 2202 86-87 0.34494114011355137 2202 88-89 0.3131917436253602 2202 90-91 0.3062963341102467 2202 92-93 0.3153628817472409 2202 94-95 0.3872563120170618 2202 96-97 0.37459677307943906 2202 98-99 0.3295252051997899 2202 100-101 0.44507181328217627 1102 1 -0.09217481066535171 1102 2 -0.19169375824566615 1102 3 -0.13098858704378458 1102 4 -0.13707703826079864 1102 5 -0.23295506294289225 1102 6 -0.16547778326908968 1102 7 -0.14850145697937478 1102 8 -0.026668801457638835 1102 9 -0.08224425946507097 1102 10-11 -0.07281680457526107 1102 12-13 -0.08970814336351651 1102 14-15 -0.07837527416970858 1102 16-17 -0.10508559391385575 1102 18-19 -0.016219904773564053 1102 20-21 -0.1063871342346232 1102 22-23 -0.10272951959223775 1102 24-25 -0.14845405820948798 1102 26-27 -0.058855907145158426 1102 28-29 -0.10151840203779727 1102 30-31 -0.12375605012019975 1102 32-33 -0.08401745354382939 1102 34-35 -0.16211520101214205 1102 36-37 -0.08558599773044762 1102 38-39 -0.1448988191416163 1102 40-41 -0.2038964912676775 1102 42-43 -0.18994303672286605 1102 44-45 -0.12055094815329426 1102 46-47 -0.050763619860013875 1102 48-49 -0.17218678369816587 1102 50-51 -0.06554760316551267 1102 52-53 -0.06592544974279235 1102 54-55 0.010464681792100805 1102 56-57 0.11155405449108713 1102 58-59 0.07615671096920096 1102 60-61 0.03728029062411764 1102 62-63 0.08619311307424482 1102 64-65 -1.4362268344569884E-4 1102 66-67 0.11430792289331038 1102 68-69 0.15600387783294423 1102 70-71 0.029601387168106896 1102 72-73 0.013565004059323371 1102 74-75 0.15230946216284025 1102 76-77 0.07019796739614748 1102 78-79 0.09085337607792354 1102 80-81 0.08593941703944097 1102 82-83 0.0751868977305108 1102 84-85 0.1313267799589255 1102 86-87 0.01710141218619654 1102 88-89 -0.08043165751298886 1102 90-91 -0.10146019514101923 1102 92-93 -0.037511152605222975 1102 94-95 -0.05349737104783969 1102 96-97 0.09110099312142594 1102 98-99 0.017082571745238084 1102 100-101 -0.06402204308612625 2305 1 0.19943493472534257 2305 2 0.0730210500657158 2305 3 0.1537196365441602 2305 4 0.15069337438186636 2305 5 0.21287680711856183 2305 6 0.3081024040297109 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98-99 0.14611754394307397 1213 100-101 0.22439981819569965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3026.0 3 785.0 4 785.0 5 763.0 6 2088.0 7 9493.0 8 36893.0 9 86612.0 10 96858.0 11 60625.0 12 36345.0 13 24699.0 14 18255.0 15 15220.0 16 13951.0 17 13856.0 18 14722.0 19 15727.0 20 17605.0 21 19977.0 22 22508.0 23 25078.0 24 28164.0 25 32773.0 26 38655.0 27 46293.0 28 56698.0 29 70617.0 30 87442.0 31 111988.0 32 152227.0 33 218075.0 34 331831.0 35 560200.0 36 1092091.0 37 2028105.0 38 771328.0 39 1015.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.425203762347266 24.91621751857389 23.319399015196797 15.339179703882042 2 30.722495694539692 24.396019371114004 31.98487211338919 12.896612820957118 3 37.14820146225226 20.910906710257724 26.170623205731896 15.770268621758118 4 28.308697429115227 19.873952989656384 34.380580849942355 17.436768731286033 5 32.67875451822513 18.390038305701356 31.859667635585787 17.07153954048772 6 27.485951167984673 18.82539426056302 37.2264746986099 16.462179872842402 7 33.59558999298139 19.236107533734906 29.3199587041233 17.848343769160405 8 27.998676584267894 28.40907521288159 26.408936436231883 17.183311766618626 9 30.74785151074574 26.008445950611353 25.409109363736547 17.834593174906367 10-11 24.678771164115386 29.932539122054248 29.169554391414092 16.219135322416278 12-13 26.107060090172507 26.362001043582783 29.062626908919338 18.468311957325376 14-15 24.201888539436183 26.60468211741746 30.11664795014481 19.076781393001546 16-17 24.148154911735457 27.344664785149803 30.981169180058433 17.526011123056303 18-19 23.83183703704907 27.54445230520058 31.202224584961552 17.4214860727888 20-21 23.400168778866462 28.5798230705553 30.56264577159714 17.45736237898109 22-23 24.70392851414407 27.660247498292435 29.926332945315487 17.70949104224801 24-25 23.101277054894194 28.985840718218032 29.865352478263922 18.04752974862385 26-27 21.725346644301034 30.52668467951308 29.580595562269945 18.167373113915943 28-29 22.244067013489985 29.666708692555098 29.406879776126388 18.68234451782853 30-31 23.32817416690556 28.37939979916112 29.610544911966386 18.681881121966942 32-33 22.181733766189367 29.140024217103498 30.481708077436124 18.196533939271006 34-35 23.900772997298468 28.146645383510744 29.86034699445701 18.09223462473378 36-37 22.881217696985207 27.906825688378067 30.4849540332444 18.727002581392323 38-39 21.54252942016355 28.773923648482874 30.923427555894772 18.760119375458807 40-41 21.48707881655302 29.84828990861088 29.57960516220521 19.085026112630892 42-43 22.59879245971202 28.56493867043562 30.254257572772374 18.582011297079987 44-45 21.761999482469193 28.915255266494583 30.746889422518482 18.57585582851774 46-47 22.351408282198822 29.311115599772396 29.601562379786174 18.735913738242605 48-49 21.020795979203044 29.59610483666652 30.01480216774987 19.368297016380566 50-51 20.795979678569683 28.32733297510299 30.733264943904388 20.14342240242294 52-53 22.82272286509343 27.955137184569267 29.21532972682912 20.006810223508182 54-55 23.880763453161634 28.147079136819475 28.635801813439343 19.336355596579544 56-57 24.04759303708882 28.292089431785122 28.582734430214835 19.077583100911223 58-59 23.442618267111218 28.275399954107932 28.94302456414133 19.33895721463952 60-61 21.688228957134257 28.64095478339757 28.602186116618167 21.068630142850004 62-63 22.36006943893519 28.44021550968333 28.70378891344828 20.495926137933196 64-65 21.41741245079192 28.796256572741374 29.68678694259865 20.09954403386805 66-67 20.751728914647735 28.194668830539914 29.63233145676472 21.42127079804763 68-69 20.259221487573175 28.123876976521323 31.701756538926272 19.91514499697923 70-71 19.86835489177926 30.197101529459054 30.39416237748787 19.540381201273817 72-73 19.875936387865252 31.99021667197945 28.42783005673855 19.70601688341675 74-75 20.093354123890276 31.719632717515996 28.418086417809658 19.768926740784075 76-77 19.879040039258577 31.73533229698151 28.523745354233494 19.861882309526422 78-79 19.70553381149917 31.869577970036733 28.611215864984256 19.81367235347984 80-81 19.543669586217895 31.96057466662761 28.422002089578474 20.073753657576024 82-83 19.2766474594574 31.609358928259535 28.53190269222687 20.5820909200562 84-85 19.434752111793696 31.20555491851781 28.829291091464306 20.53040187822419 86-87 19.815657251659527 31.031037442301333 29.12989298269972 20.02341232333942 88-89 20.11319010548181 31.105418082858765 28.645608285925267 20.13578352573416 90-91 20.192813281705295 30.861152415040838 28.581349110272527 20.364685192981334 92-93 19.856754898948605 31.371342712895313 28.609858239034324 20.162044149121755 94-95 19.511728373766612 31.49585176757141 28.726802476311853 20.265617382350126 96-97 19.77329848747661 31.599056183450987 28.585801005093824 20.04184432397858 98-99 20.335325401683324 30.99934899886703 28.124388338493567 20.54093726095607 100-101 20.523799183998214 30.924037545327554 27.524727302203555 21.027435968470677 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1855.0 1 1460.0 2 817.0 3 619.5 4 730.5 5 918.0 6 1203.0 7 1559.5 8 1974.5 9 2357.5 10 2590.5 11 2657.5 12 2551.5 13 2341.0 14 2180.0 15 2070.0 16 1993.0 17 1996.0 18 2131.0 19 2435.0 20 2916.5 21 3574.5 22 4638.0 23 6132.5 24 8239.5 25 11537.0 26 16798.5 27 23739.5 28 31908.5 29 41597.5 30 52687.5 31 65092.5 32 83244.0 33 111722.0 34 148710.0 35 194919.0 36 257805.0 37 322032.0 38 377566.0 39 408759.0 40 413816.0 41 399765.5 42 371210.0 43 348941.0 44 321045.0 45 294055.0 46 279946.5 47 256564.0 48 223554.5 49 196998.5 50 168844.5 51 141418.0 52 117166.5 53 93398.0 54 76341.5 55 62324.5 56 47513.5 57 34510.5 58 25620.0 59 19566.0 60 14804.0 61 11211.5 62 8298.0 63 6135.0 64 4718.0 65 3690.0 66 2847.5 67 2117.0 68 1595.0 69 1269.5 70 967.0 71 757.5 72 602.5 73 483.0 74 407.0 75 316.0 76 244.0 77 205.0 78 163.5 79 128.5 80 107.5 81 87.5 82 73.0 83 65.0 84 60.0 85 56.5 86 46.0 87 38.0 88 29.0 89 21.0 90 16.5 91 15.5 92 12.5 93 13.0 94 13.5 95 9.0 96 6.5 97 3.0 98 2.5 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3016530072088774 2 0.2775752822358796 3 0.24861386776364175 4 0.236169383225711 5 0.24241596281776226 6 0.19135625898351438 7 0.15790055218141105 8 0.1557588677498506 9 0.13359567885961146 10-11 0.11822260311034234 12-13 0.139769246482405 14-15 0.1432413712426621 16-17 0.13645125810169204 18-19 0.12360115151232937 20-21 0.12949889614014923 22-23 0.1602369351976588 24-25 0.5757318273614139 26-27 0.2280893919611875 28-29 0.2012290997153669 30-31 0.3025372632809989 32-33 0.33434614455428874 34-35 0.342564047316299 36-37 0.3336484746258258 38-39 0.3418907147109221 40-41 0.34446235851700036 42-43 0.3563470846239551 44-45 0.33656084095510685 46-47 0.35650122100349924 48-49 0.35216106505317785 50-51 0.3581318216502555 52-53 0.34294533204464506 54-55 0.3346463048723483 56-57 0.3435132029166497 58-59 0.33584694614458677 60-61 0.3121018312537632 62-63 0.29862706670519534 64-65 0.30919757736551073 66-67 0.3256009331254169 68-69 0.3122478551922786 70-71 0.2930700446005783 72-73 0.2890624987324311 74-75 0.28090949549864985 76-77 0.28320531630975443 78-79 0.29229936270285767 80-81 0.3016692320909346 82-83 0.3248221387866676 84-85 0.31613371444499627 86-87 0.31800768832261167 88-89 0.3243597296480352 90-91 0.3172532313069483 92-93 0.2982295570947921 94-95 0.2937514896469839 96-97 0.3110391014790116 98-99 0.32057933212868994 100-101 0.3205874445697186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6163373.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.53161381824754 #Duplication Level Percentage of deduplicated Percentage of total 1 92.93055818864228 56.25236660189077 2 3.4117546313205747 4.130380275714291 3 1.105520787520097 2.0075687213463422 4 0.5466340926790608 1.3235457519174818 5 0.34383550339995367 1.040645895440437 6 0.24762796659024422 0.8993592266543141 7 0.18165689892271925 0.769718967710733 8 0.151718116630297 0.7346993956077582 9 0.10448009538324551 0.5691913906889057 >10 0.7607122886509864 9.670342162281809 >50 0.1244632713220634 5.211302478926986 >100 0.07656139894529415 9.436359548505896 >500 0.011294692229465613 4.7043130054141304 >1k 0.0031014532727358184 2.645364667147463 >5k 0.0 0.0 >10k+ 8.061449104979436E-5 0.6048419107526144 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 14021 0.2274890713250683 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 13072 0.21209165825271326 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 10092 0.16374150972203044 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.112441028638053E-4 0.0 0.0 2 8.761436310929097E-4 0.0 0.0 3 9.24818277264738E-4 0.0 0.0 4 0.0010221675696083946 0.0 0.0 5 0.0012655408004675361 0.0 1.6224882057276106E-5 6 0.0014277896210402972 0.0 1.6224882057276106E-5 7 0.001557588677498506 0.0 1.6224882057276106E-5 8 0.0018009619083576476 0.0 1.6224882057276106E-5 9 0.0019307609648158564 0.0 1.6224882057276106E-5 10-11 0.0031476271191115644 0.0 2.4337323085914157E-5 12-13 0.004315818627235444 0.0 3.244976411455221E-5 14-15 0.005532684781531152 0.0 3.244976411455221E-5 16-17 0.006952361961542811 0.0 3.244976411455221E-5 18-19 0.008355814259497194 0.0 4.867464617182831E-5 20-21 0.009929627819052976 8.112441028638053E-6 4.867464617182831E-5 22-23 0.012071312250613423 1.6224882057276106E-5 4.867464617182831E-5 24-25 0.01433468329760344 1.6224882057276106E-5 6.489952822910442E-5 26-27 0.01670351607796575 1.6224882057276106E-5 6.489952822910442E-5 28-29 0.019599657525189537 1.6224882057276106E-5 6.489952822910442E-5 30-31 0.0282475196617177 1.6224882057276106E-5 8.923685131501859E-5 32-33 0.03135458457568607 1.6224882057276106E-5 1.1357417440093274E-4 34-35 0.03468879783845631 1.6224882057276106E-5 1.1357417440093274E-4 36-37 0.03826638433208569 1.6224882057276106E-5 1.1357417440093274E-4 38-39 0.04224148043611834 1.6224882057276106E-5 1.1357417440093274E-4 40-41 0.0466465359146688 1.6224882057276106E-5 1.1357417440093274E-4 42-43 0.051343639270250235 1.6224882057276106E-5 1.2979905645820884E-4 44-45 0.05686821161075275 1.6224882057276106E-5 1.4602393851548495E-4 46-47 0.06300932946943175 1.6224882057276106E-5 1.4602393851548495E-4 48-49 0.06935325835382672 1.6224882057276106E-5 1.6224882057276106E-4 50-51 0.07537268959707615 1.6224882057276106E-5 1.703612616013991E-4 52-53 0.08236561376376214 1.6224882057276106E-5 1.7847370263003714E-4 54-55 0.09019411935639787 1.6224882057276106E-5 1.7847370263003714E-4 56-57 0.09816053644652042 1.6224882057276106E-5 1.865861436586752E-4 58-59 0.10624864015207258 1.6224882057276106E-5 1.9469858468731325E-4 60-61 0.1141826074780806 1.6224882057276106E-5 2.028110257159513E-4 62-63 0.1230738428454679 1.6224882057276106E-5 2.1092346674458936E-4 64-65 0.1327114227874899 1.6224882057276106E-5 2.1903590777322743E-4 66-67 0.1435820937658649 1.6224882057276106E-5 2.4337323085914158E-4 68-69 0.1553532456984187 1.6224882057276106E-5 2.6771055394505573E-4 70-71 0.1680167661441227 1.6224882057276106E-5 2.7582299497369377E-4 72-73 0.18152398045680507 1.6224882057276106E-5 2.7582299497369377E-4 74-75 0.19772452519099526 1.6224882057276106E-5 2.7582299497369377E-4 76-77 0.21570169451045718 1.6224882057276106E-5 2.7582299497369377E-4 78-79 0.23576376117427908 1.6224882057276106E-5 2.7582299497369377E-4 80-81 0.2581865481774347 1.6224882057276106E-5 2.839354360023318E-4 82-83 0.283327002925184 1.6224882057276106E-5 2.920478770309699E-4 84-85 0.31173677140747447 1.6224882057276106E-5 2.920478770309699E-4 86-87 0.3452005906506064 1.6224882057276106E-5 2.920478770309699E-4 88-89 0.3829964534030311 1.6224882057276106E-5 2.920478770309699E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2615 0.0 79.78403 9 CGGGAGA 3660 0.0 73.523346 4 AGAGCGG 3085 0.0 71.1658 8 CGGAAAG 2275 0.0 69.67683 5 AAGCGGC 1190 0.0 66.54332 9 GAGAGCG 3420 0.0 65.58565 7 GATCGGG 5245 0.0 62.558384 1 GATCCGG 980 0.0 62.50526 1 GTTCGGA 945 0.0 61.29192 2 TCGGGAG 4380 0.0 60.898018 3 TCGGGAA 1615 0.0 59.069664 3 ATCGGGA 5400 0.0 58.993473 2 TCGGAAA 2065 0.0 58.836006 4 AAGGGGC 3910 0.0 57.361343 9 GGTTCGG 1290 0.0 56.318962 1 GGATCGG 2290 0.0 54.534733 1 GGTCCGG 610 0.0 54.490547 1 AAAGCGG 1760 0.0 49.304203 8 GAACCGG 650 0.0 48.94568 1 GGACCGG 830 0.0 48.628826 1 >>END_MODULE