FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_35011-1.342000000358a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_35011-1.342000000358a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6163373
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC140210.2274890713250683No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG130720.21209165825271326No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA100920.16374150972203044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC26150.079.784039
CGGGAGA36600.073.5233464
AGAGCGG30850.071.16588
CGGAAAG22750.069.676835
AAGCGGC11900.066.543329
GAGAGCG34200.065.585657
GATCGGG52450.062.5583841
GATCCGG9800.062.505261
GTTCGGA9450.061.291922
TCGGGAG43800.060.8980183
TCGGGAA16150.059.0696643
ATCGGGA54000.058.9934732
TCGGAAA20650.058.8360064
AAGGGGC39100.057.3613439
GGTTCGG12900.056.3189621
GGATCGG22900.054.5347331
GGTCCGG6100.054.4905471
AAAGCGG17600.049.3042038
GAACCGG6500.048.945681
GGACCGG8300.048.6288261
Produced by FastQC (version 0.11.2)