##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_35010-1.342000000357e8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7011968 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13032061754988 33.0 33.0 33.0 33.0 33.0 2 32.08020515781019 33.0 33.0 33.0 33.0 33.0 3 32.049496803179935 33.0 33.0 33.0 33.0 33.0 4 35.806204763056535 37.0 37.0 37.0 33.0 37.0 5 35.83152048041292 37.0 37.0 37.0 33.0 37.0 6 35.89182423536445 37.0 37.0 37.0 33.0 37.0 7 35.82242389012614 37.0 37.0 37.0 33.0 37.0 8 35.77522273347511 37.0 37.0 37.0 33.0 37.0 9 35.68779435388182 37.0 37.0 37.0 33.0 37.0 10-11 35.665052093791644 37.0 37.0 37.0 33.0 37.0 12-13 35.657895044586624 37.0 37.0 37.0 33.0 37.0 14-15 37.48604699850313 40.0 37.0 40.0 33.0 40.0 16-17 37.47899427093792 40.0 37.0 40.0 33.0 40.0 18-19 37.45956862609755 40.0 37.0 40.0 33.0 40.0 20-21 37.45468433398441 40.0 37.0 40.0 33.0 40.0 22-23 37.49475760585331 40.0 37.0 40.0 33.0 40.0 24-25 37.23530341267958 40.0 37.0 40.0 33.0 40.0 26-27 37.19028245707909 40.0 37.0 40.0 33.0 40.0 28-29 37.15224991043884 40.0 37.0 40.0 33.0 40.0 30-31 37.13954363454026 40.0 37.0 40.0 33.0 40.0 32-33 37.03156225185283 40.0 37.0 40.0 33.0 40.0 34-35 37.001543289986486 40.0 37.0 40.0 33.0 40.0 36-37 36.92480477663332 40.0 37.0 40.0 33.0 40.0 38-39 36.858692737901826 40.0 37.0 40.0 33.0 40.0 40-41 36.77367053015644 40.0 37.0 40.0 33.0 40.0 42-43 36.690293153077704 40.0 37.0 40.0 33.0 40.0 44-45 36.59846593709498 40.0 37.0 40.0 33.0 40.0 46-47 36.491063992305726 40.0 37.0 40.0 33.0 40.0 48-49 36.38506386509465 38.5 37.0 40.0 33.0 40.0 50-51 35.26958765356602 37.0 35.0 38.5 30.0 38.5 52-53 35.681756248745 37.0 37.0 38.5 33.0 40.0 54-55 36.43716428825688 37.0 37.0 40.0 33.0 40.0 56-57 36.52650297320238 38.5 37.0 40.0 33.0 40.0 58-59 36.34916082047151 37.0 37.0 40.0 33.0 40.0 60-61 36.14299230971961 37.0 37.0 40.0 33.0 40.0 62-63 35.91109364731841 37.0 37.0 40.0 33.0 40.0 64-65 35.70954053127453 37.0 37.0 40.0 33.0 40.0 66-67 35.445473866965735 37.0 37.0 40.0 27.0 40.0 68-69 35.25433401863785 37.0 35.0 40.0 27.0 40.0 70-71 34.96630025978442 37.0 33.0 37.0 27.0 40.0 72-73 34.76075910500447 37.0 33.0 37.0 27.0 40.0 74-75 34.54200204564539 37.0 33.0 37.0 27.0 40.0 76-77 34.28528830992954 37.0 33.0 37.0 27.0 37.0 78-79 34.00789721230902 37.0 33.0 37.0 27.0 37.0 80-81 33.72474724642211 37.0 33.0 37.0 27.0 37.0 82-83 33.48721300496523 37.0 33.0 37.0 27.0 37.0 84-85 33.23012790417754 37.0 33.0 37.0 27.0 37.0 86-87 33.08739115751811 37.0 33.0 37.0 27.0 37.0 88-89 32.965561522813566 37.0 33.0 37.0 27.0 37.0 90-91 32.818110122579 37.0 33.0 37.0 27.0 37.0 92-93 32.6592794633404 35.0 33.0 37.0 27.0 37.0 94-95 32.52420789712674 33.0 33.0 37.0 27.0 37.0 96-97 32.385575205135 33.0 33.0 37.0 22.0 37.0 98-99 32.246786137643525 33.0 33.0 37.0 22.0 37.0 100-101 30.95405155870649 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2701623325879865 2306 2 0.29834168013747586 2306 3 0.30725962818038965 2306 4 0.4187552214133774 2306 5 0.3138197985891793 2306 6 0.310121325872764 2306 7 0.3556864090014429 2306 8 0.3764886611960421 2306 9 0.3871805269306492 2306 10-11 0.36357666993266236 2306 12-13 0.42936537787469575 2306 14-15 0.6690724194812816 2306 16-17 0.6493223704893509 2306 18-19 0.5262364240717332 2306 20-21 0.5909091390327106 2306 22-23 0.5623240840757404 2306 24-25 0.6245067534839563 2306 26-27 0.5798943954979876 2306 28-29 0.4709920417089606 2306 30-31 0.4424948980657817 2306 32-33 0.5237966049195748 2306 34-35 0.46258911976093486 2306 36-37 0.48542298893655556 2306 38-39 0.45722040516585594 2306 40-41 0.4828858759885364 2306 42-43 0.5290147103497489 2306 44-45 0.5791495296634039 2306 46-47 0.533741089380122 2306 48-49 0.5994865992613185 2306 50-51 0.5658543485397161 2306 52-53 0.5441983160924337 2306 54-55 0.5523025408379425 2306 56-57 0.548894345368268 2306 58-59 0.5787607076806793 2306 60-61 0.5398954213686196 2306 62-63 0.5056886948497308 2306 64-65 0.5382709513601611 2306 66-67 0.5990923199080527 2306 68-69 0.41979279252668533 2306 70-71 0.5754735087259988 2306 72-73 0.5653206999526148 2306 74-75 0.5053981953727771 2306 76-77 0.5103041038868312 2306 78-79 0.5505808082513894 2306 80-81 0.5040178012158023 2306 82-83 0.5691205547875811 2306 84-85 0.6684970431026969 2306 86-87 0.6505956638864419 2306 88-89 0.6094186481158701 2306 90-91 0.4658775246701339 2306 92-93 0.3877289485854334 2306 94-95 0.3506155421593107 2306 96-97 0.5077625538987363 2306 98-99 0.5616498049279457 2306 100-101 0.4203538408010452 2307 1 0.0816346115482105 2307 2 0.09887542302490004 2307 3 0.08485398295984226 2307 4 0.11635428626765076 2307 5 0.10518995944234177 2307 6 0.1548483787813666 2307 7 0.16887791893506687 2307 8 0.153003795263011 2307 9 0.18864183971687254 2307 10-11 0.11584017348505427 2307 12-13 0.17874378171655536 2307 14-15 0.30448577349147854 2307 16-17 0.3296987373088385 2307 18-19 0.3386033131402115 2307 20-21 0.3169042709118983 2307 22-23 0.30125170755096775 2307 24-25 0.37001517703596676 2307 26-27 0.2582487440146366 2307 28-29 0.22887626286441787 2307 30-31 0.2134043409742148 2307 32-33 0.26985982179781587 2307 34-35 0.20994510280591783 2307 36-37 0.2956258911064893 2307 38-39 0.2330787807472774 2307 40-41 0.18118677866194588 2307 42-43 0.1962185202332094 2307 44-45 0.20766091570578027 2307 46-47 0.2191997262070089 2307 48-49 0.191663863544143 2307 50-51 0.06278530904839386 2307 52-53 0.06848319708768713 2307 54-55 0.15686112583701117 2307 56-57 0.060724450483164105 2307 58-59 0.19971575987320733 2307 60-61 0.13071086939435617 2307 62-63 0.25278079832343536 2307 64-65 0.18915613192726255 2307 66-67 0.23206948923806436 2307 68-69 0.1772702710839269 2307 70-71 0.16202135706212317 2307 72-73 0.09658453708313175 2307 74-75 0.14152257748291674 2307 76-77 0.20271010106606013 2307 78-79 0.18946536039111805 2307 80-81 0.2490196189236471 2307 82-83 0.2682657334522247 2307 84-85 0.24939230972346849 2307 86-87 0.26739474140585884 2307 88-89 0.14899351780059789 2307 90-91 0.31801684091949767 2307 92-93 0.23709326960771193 2307 94-95 0.1889231951051542 2307 96-97 0.22510041889767507 2307 98-99 0.16696858938339432 2307 100-101 0.13386155006027067 2201 1 0.21917246171508253 2201 2 0.18921884259819421 2201 3 0.2003658415115126 2201 4 0.2730588475116207 2201 5 0.1769096961740786 2201 6 0.2400268113147277 2201 7 0.31750487983011055 2201 8 0.3005469537512582 2201 9 0.3764041866357175 2201 10-11 0.3508281053859079 2201 12-13 0.38603809875359474 2201 14-15 0.6163314407693719 2201 16-17 0.5133223404531293 2201 18-19 0.5446641136260624 2201 20-21 0.5087938900281657 2201 22-23 0.49409799582357294 2201 24-25 0.5698367275971208 2201 26-27 0.4611776831891916 2201 28-29 0.4400728884335834 2201 30-31 0.3978908683515172 2201 32-33 0.4283773953684644 2201 34-35 0.3936338725585671 2201 36-37 0.37463660000486954 2201 38-39 0.39996070384408 2201 40-41 0.3569370560326064 2201 42-43 0.421391511616406 2201 44-45 0.46407700904851623 2201 46-47 0.4081070170262251 2201 48-49 0.45669245473947484 2201 50-51 0.35564420800561436 2201 52-53 0.4393245289949448 2201 54-55 0.4955158890048992 2201 56-57 0.4900217998806795 2201 58-59 0.5083095972936675 2201 60-61 0.44820530225810273 2201 62-63 0.5157344629730929 2201 64-65 0.5105287830111536 2201 66-67 0.5896246015121918 2201 68-69 0.5386000353748202 2201 70-71 0.4435173167324038 2201 72-73 0.40737439662756003 2201 74-75 0.42186367134489444 2201 76-77 0.4037767001322692 2201 78-79 0.46135306918790775 2201 80-81 0.3917721662980469 2201 82-83 0.38653558052845227 2201 84-85 0.5057265926082195 2201 86-87 0.46574481062977924 2201 88-89 0.435916700739007 2201 90-91 0.3974984077453456 2201 92-93 0.3192532685643954 2201 94-95 0.35348254646917354 2201 96-97 0.3520389946800364 2201 98-99 0.3939607112677308 2201 100-101 0.2871989417826697 1101 1 0.16208716860010242 1101 2 0.11470811097438371 1101 3 0.05525264556636955 1101 4 0.17001886521446608 1101 5 0.12241393608002227 1101 6 0.12765286658455466 1101 7 0.287330976634415 1101 8 0.22630566042426636 1101 9 0.27177668345184713 1101 10-11 0.2407434810513962 1101 12-13 0.23328407151635844 1101 14-15 0.37129722261254017 1101 16-17 0.29986515384367607 1101 18-19 0.339838293428663 1101 20-21 0.3518669000587167 1101 22-23 0.36085329979717784 1101 24-25 0.3769831349078885 1101 26-27 0.3192448873711413 1101 28-29 0.29374461338611013 1101 30-31 0.34345321743059287 1101 32-33 0.23479901293667638 1101 34-35 0.23576663634234762 1101 36-37 0.2950032098729949 1101 38-39 0.22524879500443973 1101 40-41 0.2134029089825802 1101 42-43 0.27598900077877175 1101 44-45 0.33019850667650275 1101 46-47 0.23850933090815118 1101 48-49 0.2665571765844419 1101 50-51 0.08386771391558057 1101 52-53 0.11296411541114537 1101 54-55 0.31406494531192664 1101 56-57 0.2866326339504184 1101 58-59 0.4310332310800291 1101 60-61 0.3792393443133548 1101 62-63 0.3332272719176643 1101 64-65 0.3772780407343532 1101 66-67 0.4031212733952927 1101 68-69 0.3467905975472192 1101 70-71 0.3178323525913811 1101 72-73 0.21162352763293057 1101 74-75 0.26838134247115875 1101 76-77 0.3002087751793425 1101 78-79 0.3581680854961249 1101 80-81 0.4191290236728591 1101 82-83 0.3660032744349664 1101 84-85 0.34108306649869746 1101 86-87 0.2870220292561143 1101 88-89 0.3295192030032723 1101 90-91 0.2777337002955207 1101 92-93 0.23135983144699424 1101 94-95 0.3219206486503694 1101 96-97 0.24852729623543013 1101 98-99 0.3597549365844799 1101 100-101 0.33403993162410117 2304 1 0.10106250559726249 2304 2 0.12142885002700865 2304 3 0.06953464913516427 2304 4 0.15958342135148484 2304 5 0.1331286311893365 2304 6 0.17761765578401167 2304 7 0.23038104566025908 2304 8 0.22542583586650977 2304 9 0.319382339116153 2304 10-11 0.24420779024755745 2304 12-13 0.24119031674627678 2304 14-15 0.3418950657753044 2304 16-17 0.23227566129299504 2304 18-19 0.31545835295342073 2304 20-21 0.28412441821301115 2304 22-23 0.21578554400115735 2304 24-25 0.19166583085346645 2304 26-27 0.1502156720087413 2304 28-29 0.16074321386320634 2304 30-31 0.21233822392533597 2304 32-33 0.1535355638858249 2304 34-35 0.1438018063589439 2304 36-37 0.2313609005275481 2304 38-39 0.15723973265311741 2304 40-41 0.18054040255564274 2304 42-43 0.1867226426050479 2304 44-45 0.19806421147556819 2304 46-47 0.2019147963925647 2304 48-49 0.1770446298784023 2304 50-51 0.16976152677310097 2304 52-53 0.23434253559548068 2304 54-55 0.37997234753885323 2304 56-57 0.3582170724594107 2304 58-59 0.24630085989146977 2304 60-61 0.2854131033281462 2304 62-63 0.3038714919712078 2304 64-65 0.34430318047380837 2304 66-67 0.4015711864522373 2304 68-69 0.30457703498434796 2304 70-71 0.26177318458785237 2304 72-73 0.27314739802154975 2304 74-75 0.3477980310641442 2304 76-77 0.28069811126619015 2304 78-79 0.38451986049862086 2304 80-81 0.2878219397587074 2304 82-83 0.23539728834149543 2304 84-85 0.3158124620429916 2304 86-87 0.2987846837129453 2304 88-89 0.2818960755754176 2304 90-91 0.25784949700694426 2304 92-93 0.2755610669630073 2304 94-95 0.3209361419965475 2304 96-97 0.3365203439583908 2304 98-99 0.2764024486548422 2304 100-101 0.35308138217049745 2202 1 0.15761027655170068 2202 2 0.1443185067333772 2202 3 0.18958012790530887 2202 4 0.2409963523903471 2202 5 0.22858235457806586 2202 6 0.31728125813860686 2202 7 0.2710663852624222 2202 8 0.26523508085888636 2202 9 0.33239192541308427 2202 10-11 0.33819733762470605 2202 12-13 0.3186732927160847 2202 14-15 0.4031003666079158 2202 16-17 0.4039207253359933 2202 18-19 0.43878228310089895 2202 20-21 0.4124843632244577 2202 22-23 0.32921761598212385 2202 24-25 0.344875421835809 2202 26-27 0.33775980743480716 2202 28-29 0.30670728329504726 2202 30-31 0.3723393296585229 2202 32-33 0.23370948882678988 2202 34-35 0.29008891081103627 2202 36-37 0.3475161967742224 2202 38-39 0.32342148306140217 2202 40-41 0.27593209918236283 2202 42-43 0.34946386739932933 2202 44-45 0.3926063849378423 2202 46-47 0.370913731664146 2202 48-49 0.3629910343022189 2202 50-51 0.2511321020551378 2202 52-53 0.3694007622162019 2202 54-55 0.5279649355634746 2202 56-57 0.4950060654616095 2202 58-59 0.48499691305051584 2202 60-61 0.46408432381024767 2202 62-63 0.4777883715375353 2202 64-65 0.4546628641355426 2202 66-67 0.493571423632595 2202 68-69 0.4971472235804413 2202 70-71 0.5221810392009232 2202 72-73 0.45621641688317993 2202 74-75 0.4743428099473448 2202 76-77 0.4279500599650419 2202 78-79 0.46586301514172845 2202 80-81 0.42323592207547023 2202 82-83 0.5413480628461329 2202 84-85 0.5332867815828379 2202 86-87 0.6219071768289055 2202 88-89 0.4908951313968615 2202 90-91 0.500095609875288 2202 92-93 0.462541973260997 2202 94-95 0.41100112341776907 2202 96-97 0.506875064291485 2202 98-99 0.4716894777187335 2202 100-101 0.5898776764559805 1102 1 0.055873182928216636 1102 2 0.04713282485892023 1102 3 0.05524870603986187 1102 4 0.05850398203612173 1102 5 0.07205408679337211 1102 6 0.08344179498630666 1102 7 0.05552235765600955 1102 8 0.080925960627944 1102 9 0.0964896272071627 1102 10-11 0.06595162916818254 1102 12-13 0.07377408393468698 1102 14-15 0.046608210010759876 1102 16-17 0.0738894448465075 1102 18-19 0.07568651464423937 1102 20-21 0.09425129553542888 1102 22-23 0.05930871141301708 1102 24-25 0.1512206601326156 1102 26-27 0.09872756874224109 1102 28-29 0.0884082895405669 1102 30-31 0.09006154970554547 1102 32-33 0.02798083913652505 1102 34-35 0.03255218851109021 1102 36-37 0.08059277471407 1102 38-39 0.04923424933181053 1102 40-41 -0.008626188242580213 1102 42-43 0.00424537222790633 1102 44-45 0.0812665593509152 1102 46-47 0.04860613154224325 1102 48-49 0.12337790813687377 1102 50-51 0.1375581976659248 1102 52-53 0.18290273523625444 1102 54-55 0.25167351748640243 1102 56-57 0.27321616484806555 1102 58-59 0.2447419601299785 1102 60-61 0.2718542608894765 1102 62-63 0.30397281848875224 1102 64-65 0.20403971466856063 1102 66-67 0.26469804241909145 1102 68-69 0.28129164769562465 1102 70-71 0.2592714087043433 1102 72-73 0.26561994207582273 1102 74-75 0.2371391333258117 1102 76-77 0.2592297028849231 1102 78-79 0.23856585419535747 1102 80-81 0.28564413616692264 1102 82-83 0.27994963925157634 1102 84-85 0.32464468657311585 1102 86-87 0.2634476086605062 1102 88-89 0.14638187581324758 1102 90-91 0.08962783639852034 1102 92-93 0.13019411379463008 1102 94-95 0.14887876971966563 1102 96-97 0.09768661891910568 1102 98-99 0.2097338571777314 1102 100-101 0.14484493564594558 2305 1 0.08933995954651408 2305 2 0.04932890327185646 2305 3 0.07950608593150577 2305 4 0.10526069902101653 2305 5 0.10799952345615793 2305 6 0.11399340392210178 2305 7 0.1628567626102324 2305 8 0.19433684621494507 2305 9 0.0658183894615405 2305 10-11 0.16579304871010692 2305 12-13 0.16129059783504118 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1213 72-73 6.36466425127935E-4 1213 74-75 -0.0026487185839485505 1213 76-77 -0.01888386537229536 1213 78-79 -0.003546118002645926 1213 80-81 0.017458018624800786 1213 82-83 0.05909131268332857 1213 84-85 0.07204996283918064 1213 86-87 -0.03166937721800167 1213 88-89 -0.04363555054753476 1213 90-91 0.002455567238285994 1213 92-93 -0.03479788085343927 1213 94-95 0.1075556190937661 1213 96-97 0.0870255321957174 1213 98-99 0.01096992611146419 1213 100-101 0.12439382967964008 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3547.0 3 700.0 4 683.0 5 751.0 6 1871.0 7 4586.0 8 14592.0 9 28537.0 10 32647.0 11 20959.0 12 13730.0 13 11040.0 14 10613.0 15 10934.0 16 11530.0 17 11883.0 18 12580.0 19 14196.0 20 16272.0 21 18607.0 22 21709.0 23 24668.0 24 27925.0 25 32923.0 26 40037.0 27 49162.0 28 61270.0 29 76556.0 30 96392.0 31 124685.0 32 170033.0 33 246851.0 34 378398.0 35 641408.0 36 1250588.0 37 2426716.0 38 1101519.0 39 870.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.39362755399315 26.770315821306916 21.47921349592447 16.35684312877546 2 32.00658917040268 24.41954674711198 29.96885475693281 13.605009325552528 3 36.1453343625687 21.31741078200775 25.255966059824807 17.281288795598744 4 30.914751276224084 20.91040391568379 30.27289730207751 17.90194750601461 5 29.73646624872222 19.580115947416203 32.68416123982244 17.999256564039147 6 26.429072672966008 19.717068309618753 35.53018684402791 18.323672173387326 7 32.168186676772294 19.72735379684464 28.00365848031938 20.100801046063683 8 29.901822216121392 27.337327026879944 23.632573718327336 19.12827703867133 9 27.485291341263444 27.06744518759306 25.576343215651686 19.870920255491807 10-11 23.341755642793842 29.413528969082048 29.99036828202664 17.254347106097466 12-13 23.61523756749056 27.47771499327683 30.193368804632453 18.713678634600157 14-15 22.48763061630006 27.691444710452696 31.14718825707466 18.673736416172588 16-17 22.10423174072247 27.976690197402398 32.0210590551988 17.898019006676336 18-19 21.88851329112035 28.08328474742252 31.927055019813483 18.101146941643645 20-21 21.767525078463468 28.75746398991692 31.2530577832132 18.22195314840641 22-23 22.254508864883256 28.764434531483307 30.495114125631545 18.48594247800189 24-25 21.799527628103927 28.973601384193536 30.516337671095666 18.710533316606874 26-27 21.458916390738477 29.46548563421124 30.395058744168953 18.680539230881337 28-29 21.683492993549926 29.321295633958133 30.152561983955305 18.842649388536632 30-31 21.878460065263013 29.10414392741312 30.161200459483435 18.856195547840432 32-33 21.401701977244482 29.374849289936467 30.35057039063333 18.87287834218572 34-35 22.054403754093318 28.971233106390425 30.25504760777522 18.71931553174103 36-37 21.533597999768023 28.778750418855637 30.706556057534364 18.981095523841983 38-39 21.31754929328115 29.200345289293562 30.52208654287002 18.960018874555264 40-41 21.35425380767909 29.624220641794853 29.830324864795315 19.191200685730742 42-43 21.495472181843024 29.040831416042035 30.206693866473795 19.25700253564115 44-45 21.143693400993293 29.05242215502213 30.48748069702721 19.316403746957373 46-47 21.398215970807964 29.308428351657085 29.918291935367858 19.375063742167097 48-49 21.08295808153532 29.600503548312297 29.73497155911039 19.58156681104199 50-51 20.980683651319318 29.239453885703977 29.915150694459403 19.864711768517303 52-53 21.60071674738662 29.078039306609877 29.45391663518016 19.86732731082334 54-55 21.796211425885474 29.250149745941727 29.24736627102445 19.706272557148356 56-57 21.77099397243335 29.185251604214358 29.40278214099912 19.64097228235318 58-59 21.625160891645255 29.208692170240464 29.534131926426472 19.63201501168781 60-61 20.94809556199142 29.313854623048414 29.43327992793562 20.30476988702455 62-63 21.010242173239654 29.173724399126815 29.488655257608638 20.327378170024897 64-65 20.79310777499946 29.26686840907592 29.728450386804894 20.211573429119724 66-67 20.68203874534257 28.99906386361887 29.713622687527064 20.605274703511498 68-69 20.49582600455712 29.120833721725386 30.269255667227263 20.114084606490234 70-71 20.291764334969958 29.90258286335845 29.7252475266999 20.080405274971692 72-73 20.303853395571423 30.33937333455586 29.241619550156106 20.115153719716613 74-75 20.417106894994692 30.34179978165342 29.115570502301537 20.12552282105035 76-77 20.449857575733738 30.291013218316408 29.143301768521056 20.115827437428795 78-79 20.319829546307947 30.251110216278498 29.402367841527848 20.02669239588571 80-81 20.137580587360524 30.58843347398494 29.26148966714997 20.012496271504567 82-83 20.0589750794015 30.41188900052666 29.25933215809597 20.26980376197587 84-85 20.198205651294153 29.981918869134503 29.451847131242655 20.368028348328686 86-87 20.339212592837004 29.98302871053971 29.552693986517404 20.12506471010588 88-89 20.576470546149196 30.245876246248287 29.108224612665857 20.069428594936664 90-91 20.6323623004431 30.097150029246023 29.05902345748681 20.21146421282407 92-93 20.373840104950524 30.22527583237529 29.053959667063477 20.346924395610717 94-95 20.140521135389832 30.28069323168857 28.979607105005407 20.59917852791619 96-97 20.240102458748126 30.57242308458033 28.821602067545772 20.36587238912577 98-99 20.51857479928514 30.52027037661849 28.669479751086385 20.291675073009984 100-101 20.641415344291044 30.32344351069327 28.369357950061357 20.665783194954322 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2034.0 1 1602.0 2 848.0 3 528.0 4 532.0 5 656.5 6 978.5 7 1373.0 8 1823.0 9 2330.0 10 2604.0 11 2560.5 12 2389.0 13 2090.0 14 1695.5 15 1391.5 16 1207.5 17 1134.5 18 1198.0 19 1301.0 20 1536.5 21 2003.5 22 2876.0 23 4464.5 24 6831.5 25 10712.5 26 17671.0 27 27179.0 28 38647.5 29 52832.0 30 69570.0 31 88894.5 32 114448.0 33 149805.5 34 192786.0 35 240999.5 36 296338.5 37 355809.0 38 411418.5 39 446391.0 40 462333.5 41 455861.5 42 429281.5 43 406833.5 44 377437.5 45 348015.5 46 332210.0 47 304959.0 48 262640.0 49 223710.0 50 183875.5 51 147929.0 52 120181.0 53 95613.0 54 78321.5 55 62559.0 56 45219.0 57 31323.0 58 22399.5 59 16560.5 60 12194.5 61 8824.5 62 6365.5 63 4433.5 64 3113.5 65 2230.0 66 1622.5 67 1219.0 68 918.0 69 698.0 70 521.0 71 413.0 72 335.0 73 250.0 74 197.0 75 156.5 76 126.5 77 114.5 78 91.5 79 64.5 80 49.5 81 47.0 82 39.5 83 33.0 84 30.5 85 23.0 86 16.0 87 17.5 88 16.0 89 13.0 90 12.0 91 11.5 92 11.0 93 6.0 94 4.0 95 3.0 96 2.0 97 2.5 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3079164080611891 2 0.28364362187619796 3 0.253552212445921 4 0.24128746737007356 5 0.24840387178036183 6 0.19496666271152407 7 0.16039719519541445 8 0.15825799547288294 9 0.13733662218652454 10-11 0.12185594686113799 12-13 0.1435117787188989 14-15 0.1469701516036582 16-17 0.14046698444716232 18-19 0.12705420218688962 20-21 0.13328640404519815 22-23 0.16373434676256365 24-25 0.5843937108669064 26-27 0.23299450311239298 28-29 0.20609763193442984 30-31 0.31295778874062175 32-33 0.3453096192110403 34-35 0.3539876394187766 36-37 0.34481760327485805 38-39 0.3534457088224019 40-41 0.35609118581259924 42-43 0.36746459767072526 44-45 0.34766986957156676 46-47 0.3681634029134189 48-49 0.364348496741571 50-51 0.37071618124897315 52-53 0.3543655647030905 54-55 0.34654322438436685 56-57 0.3551499379346854 58-59 0.3472063762983516 60-61 0.3231902940800643 62-63 0.3095208078530877 64-65 0.3212650143297859 66-67 0.33756571621547615 68-69 0.32346839004399336 70-71 0.3031602540114273 72-73 0.2989888145524908 74-75 0.2899471303919242 76-77 0.29282791935159996 78-79 0.3025256247604096 80-81 0.3120807168543838 82-83 0.33646046302550153 84-85 0.3267841496139172 86-87 0.32952232525875763 88-89 0.33504146054288897 90-91 0.3277824428177653 92-93 0.3084226853288549 94-95 0.30443664317920444 96-97 0.3225699261605301 98-99 0.3308343677552436 100-101 0.3315973489896132 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7011968.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.95435611992959 #Duplication Level Percentage of deduplicated Percentage of total 1 92.13288686234847 61.68698117338861 2 4.339179304407968 5.810539128311188 3 1.2014855073050765 2.4133406558711505 4 0.5510047183986599 1.475686645577016 5 0.31712791693478193 1.061654774301142 6 0.22491630159988235 0.9035475692697607 7 0.1381571867979982 0.6475157839780564 8 0.11182962854321707 0.5989984619393598 9 0.11367144851995688 0.6849718780386091 >10 0.7219219156379973 9.57991760079005 >50 0.08234788535889101 3.8852468939019307 >100 0.058947614948958275 7.816542673262446 >500 0.005386857536780189 2.4145547592706413 >1k 0.0011368516616268864 1.020502002100005 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.128958945619832E-4 0.0 0.0 2 9.269865464303317E-4 0.0 0.0 3 9.982932038480495E-4 0.0 0.0 4 0.0010553385297822238 0.0 0.0 5 0.0012264745075847466 0.0 0.0 6 0.0015117011372556177 0.0 0.0 7 0.0019110184187948376 0.0 0.0 8 0.002267551705883427 0.0 0.0 9 0.0026668689874226464 0.0 1.4261331483543564E-5 10-11 0.004820330041437724 0.0 4.991466019240248E-5 12-13 0.006931007101002172 0.0 8.556798890126138E-5 14-15 0.010054238695898212 0.0 1.0695998612657672E-4 16-17 0.014004627516839781 0.0 1.1409065186834851E-4 18-19 0.017748227031269964 0.0 1.1409065186834851E-4 20-21 0.01968776811303189 0.0 1.1409065186834851E-4 22-23 0.021477565214216607 0.0 1.1409065186834851E-4 24-25 0.02364528759971523 0.0 1.1409065186834851E-4 26-27 0.025984145963016375 0.0 1.2835198335189207E-4 28-29 0.029064593563461784 0.0 1.3548264909366386E-4 30-31 0.04763997782077728 1.4261331483543564E-5 1.7113597780252278E-4 32-33 0.052446046530731455 1.4261331483543564E-5 1.7113597780252278E-4 34-35 0.056118339387743923 1.4261331483543564E-5 1.7113597780252278E-4 36-37 0.06056074414486774 1.4261331483543564E-5 1.7826664354429457E-4 38-39 0.06559499415855863 1.4261331483543564E-5 1.8539730928606634E-4 40-41 0.0712353507603001 2.1391997225315344E-5 1.8539730928606634E-4 42-43 0.07734633130099852 2.8522662967087128E-5 1.8539730928606634E-4 44-45 0.08413472508716527 2.8522662967087128E-5 1.8539730928606634E-4 46-47 0.0913010441576459 2.8522662967087128E-5 1.8539730928606634E-4 48-49 0.09938008844307333 2.8522662967087128E-5 1.8539730928606634E-4 50-51 0.10791549533597415 3.565332870885891E-5 1.8539730928606634E-4 52-53 0.11735649677808 4.2783994450630695E-5 1.925279750278381E-4 54-55 0.1276888314379073 4.2783994450630695E-5 2.1391997225315347E-4 56-57 0.13881266999507128 4.2783994450630695E-5 2.3531196947846881E-4 58-59 0.15152664701265037 4.2783994450630695E-5 2.4957330096201237E-4 60-61 0.16522465590259397 4.2783994450630695E-5 2.852266296708713E-4 62-63 0.17947885672039576 4.2783994450630695E-5 2.994879611544149E-4 64-65 0.19612896122743287 4.2783994450630695E-5 3.208799583797302E-4 66-67 0.21392710291889522 4.2783994450630695E-5 3.28010624121502E-4 68-69 0.23393575099030686 4.2783994450630695E-5 3.4227195560504556E-4 70-71 0.25624760409631075 4.2783994450630695E-5 3.4227195560504556E-4 72-73 0.28158285947682593 4.2783994450630695E-5 3.565332870885891E-4 74-75 0.31106103165331045 4.2783994450630695E-5 3.565332870885891E-4 76-77 0.34457516063963783 4.2783994450630695E-5 3.565332870885891E-4 78-79 0.3810699079060258 4.2783994450630695E-5 3.565332870885891E-4 80-81 0.4240179076687173 4.2783994450630695E-5 3.636639528303609E-4 82-83 0.4713370055311148 4.2783994450630695E-5 3.8505595005567625E-4 84-85 0.5252947531991019 4.2783994450630695E-5 3.8505595005567625E-4 86-87 0.5876239024479291 4.2783994450630695E-5 3.8505595005567625E-4 88-89 0.6579607893247659 4.2783994450630695E-5 4.1357861302276336E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1455 0.0 51.164825 9 AGAGCGG 1555 0.0 49.094566 8 CGGAAAG 1210 0.0 47.07127 5 CGGGAGA 2010 0.0 46.991257 4 GATCGGG 2830 0.0 46.4775 1 GAGAGCG 1835 0.0 43.15558 7 GGTCCGG 430 0.0 40.85853 1 AAGCGGC 785 0.0 40.47063 9 ATCGGGA 3030 0.0 39.640614 2 TTGTGTT 55870 0.0 39.571613 1 GGTTCGG 805 0.0 38.34131 1 AGCGGCG 1335 0.0 36.05209 10-11 TCGGGAA 1110 0.0 35.492264 3 GTTCGGA 650 0.0 35.05826 2 TGTGTTT 99825 0.0 35.047718 1 GGCGCCG 1305 0.0 34.943928 56-57 GATCCGG 570 0.0 34.155327 1 CGCCGTA 1085 0.0 33.925323 58-59 TCGGGAG 2895 0.0 32.79141 3 CGCCGGA 775 0.0 32.78718 58-59 >>END_MODULE