##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_35008-2.342000000358b0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5937612 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.53876524771238 33.0 33.0 33.0 27.0 33.0 2 31.291432313192576 33.0 33.0 33.0 27.0 33.0 3 31.207277774297143 33.0 33.0 33.0 27.0 33.0 4 34.79161723602014 37.0 37.0 37.0 33.0 37.0 5 34.83500622809304 37.0 37.0 37.0 33.0 37.0 6 35.07215830202445 37.0 37.0 37.0 33.0 37.0 7 34.83937515620758 37.0 37.0 37.0 33.0 37.0 8 34.884990295762 37.0 37.0 37.0 33.0 37.0 9 34.6271405069917 37.0 37.0 37.0 33.0 37.0 10-11 34.63083357417089 37.0 37.0 37.0 33.0 37.0 12-13 34.59167591280804 37.0 37.0 37.0 33.0 37.0 14-15 36.265375288920865 40.0 37.0 40.0 33.0 40.0 16-17 36.21993387240527 40.0 37.0 40.0 33.0 40.0 18-19 36.20934653527378 40.0 37.0 40.0 33.0 40.0 20-21 36.18499996294807 40.0 37.0 40.0 30.0 40.0 22-23 36.47140929720568 40.0 37.0 40.0 33.0 40.0 24-25 36.03860036661203 40.0 37.0 40.0 27.0 40.0 26-27 35.93906658097565 40.0 37.0 40.0 27.0 40.0 28-29 35.80346425465322 40.0 37.0 40.0 27.0 40.0 30-31 35.79613639287983 40.0 37.0 40.0 27.0 40.0 32-33 35.688859932242124 40.0 37.0 40.0 27.0 40.0 34-35 35.64143741625421 40.0 37.0 40.0 27.0 40.0 36-37 35.54906459027636 40.0 37.0 40.0 27.0 40.0 38-39 35.426227749472346 38.5 37.0 40.0 27.0 40.0 40-41 35.38954052571977 37.0 37.0 40.0 27.0 40.0 42-43 35.283552967085086 37.0 37.0 40.0 27.0 40.0 44-45 35.148812771868556 37.0 37.0 40.0 22.0 40.0 46-47 35.03551217223355 37.0 37.0 40.0 22.0 40.0 48-49 34.930075171634655 37.0 37.0 40.0 22.0 40.0 50-51 33.92483964934051 37.0 35.0 38.5 22.0 38.5 52-53 34.332399708839176 37.0 35.0 38.5 22.0 40.0 54-55 35.02617449574004 37.0 37.0 40.0 22.0 40.0 56-57 35.199377291746245 37.0 37.0 40.0 24.5 40.0 58-59 34.91552201457421 37.0 37.0 40.0 22.0 40.0 60-61 34.743899651913935 37.0 35.0 40.0 22.0 40.0 62-63 34.491725882391776 37.0 33.0 40.0 22.0 40.0 64-65 34.32300426501428 37.0 33.0 40.0 22.0 40.0 66-67 34.10817690681035 37.0 33.0 40.0 22.0 40.0 68-69 34.02183680914145 37.0 33.0 40.0 22.0 40.0 70-71 33.714741465087315 37.0 33.0 38.5 22.0 40.0 72-73 33.66917146152359 37.0 33.0 37.0 22.0 40.0 74-75 33.575638404799776 37.0 33.0 37.0 22.0 40.0 76-77 33.300808220543885 37.0 33.0 37.0 22.0 40.0 78-79 32.942851856941815 37.0 33.0 37.0 22.0 37.0 80-81 32.53175796936546 37.0 33.0 37.0 22.0 37.0 82-83 32.20795153000903 37.0 33.0 37.0 18.5 37.0 84-85 31.897117056486685 37.0 33.0 37.0 15.0 37.0 86-87 31.716477095505738 37.0 33.0 37.0 15.0 37.0 88-89 31.5516383522534 33.0 33.0 37.0 15.0 37.0 90-91 31.37802537451083 33.0 33.0 37.0 15.0 37.0 92-93 31.194888871148873 33.0 33.0 37.0 15.0 37.0 94-95 31.03454402207487 33.0 33.0 37.0 15.0 37.0 96-97 30.882572909782585 33.0 33.0 37.0 15.0 37.0 98-99 30.72262620056683 33.0 27.0 37.0 15.0 37.0 100-101 29.44263384000167 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.19718369220550258 2306 2 0.22181425289842593 2306 3 0.23671640106772074 2306 4 0.3765389979568212 2306 5 0.2922579325831336 2306 6 0.21547676288081874 2306 7 0.3740739913485811 2306 8 0.3001351982390972 2306 9 0.41562947070340783 2306 10-11 0.3610211455717618 2306 12-13 0.3769909798832174 2306 14-15 0.5405111377598502 2306 16-17 0.5402352394843106 2306 18-19 0.4429419447207863 2306 20-21 0.41470019307544703 2306 22-23 0.3286773099867162 2306 24-25 0.416292267389629 2306 26-27 0.3255424260945645 2306 28-29 0.24754670330586492 2306 30-31 0.13028698800034988 2306 32-33 0.21458150010573007 2306 34-35 0.3155771230113871 2306 36-37 0.34162193620350934 2306 38-39 0.29682769447637725 2306 40-41 0.2644214185121214 2306 42-43 0.3487517765628141 2306 44-45 0.44821146894769015 2306 46-47 0.34351336690014733 2306 48-49 0.35763785703911566 2306 50-51 0.27965777162169303 2306 52-53 0.27030978208747314 2306 54-55 0.3257528701563501 2306 56-57 0.32739669451284215 2306 58-59 0.3339503062679796 2306 60-61 0.3173435061524472 2306 62-63 0.3790583400098342 2306 64-65 0.3815841430858953 2306 66-67 0.3121408687678482 2306 68-69 0.14215766895625137 2306 70-71 0.30159704495874706 2306 72-73 0.18489896398147465 2306 74-75 0.20656372338108753 2306 76-77 0.18817823826971392 2306 78-79 0.2909359482747007 2306 80-81 0.30618226663877834 2306 82-83 0.3905577944616141 2306 84-85 0.5424718879936101 2306 86-87 0.40944138794041507 2306 88-89 0.2825128450154253 2306 90-91 0.25367433344351653 2306 92-93 0.2607016105914326 2306 94-95 0.281165974661306 2306 96-97 0.2671419580131129 2306 98-99 0.2723194827625868 2306 100-101 0.12595035076800443 2307 1 0.056225903035226565 2307 2 0.15669910826047584 2307 3 0.11004226459077415 2307 4 0.1803836090758324 2307 5 0.14177240406021951 2307 6 -0.03390195704903931 2307 7 0.20090829709245384 2307 8 0.07357250533225113 2307 9 0.21062444175057493 2307 10-11 0.08765327808371381 2307 12-13 0.1365260719874115 2307 14-15 0.2723606785146728 2307 16-17 0.23109288856821308 2307 18-19 0.23447483977891181 2307 20-21 0.2087111574546583 2307 22-23 0.1530157955503526 2307 24-25 0.1345996036249275 2307 26-27 -0.012457855943814877 2307 28-29 -0.01867286302445592 2307 30-31 0.05069315070357305 2307 32-33 0.11135663266102114 2307 34-35 -0.008589615445210086 2307 36-37 0.05205343297679832 2307 38-39 -0.04773211988310777 2307 40-41 0.032907882041648406 2307 42-43 0.02904423499919062 2307 44-45 0.034014293391969375 2307 46-47 0.02507414754287396 2307 48-49 -0.0977918481868798 2307 50-51 -0.25332861975210363 2307 52-53 -0.1557991802033385 2307 54-55 -0.1383705583987762 2307 56-57 -0.3243271690617888 2307 58-59 -0.0637392287363312 2307 60-61 -0.09666175710075464 2307 62-63 -0.020521977003497227 2307 64-65 -0.07776564870503222 2307 66-67 0.028098616784660635 2307 68-69 -0.027553878210824223 2307 70-71 -0.11764911144525314 2307 72-73 -0.23626826337822848 2307 74-75 -0.18783959808724404 2307 76-77 -0.17420467327723088 2307 78-79 -0.14290223741739538 2307 80-81 0.03219696007019479 2307 82-83 -0.04904058617717055 2307 84-85 -0.06098239627364066 2307 86-87 -0.013093368900246816 2307 88-89 -0.2073847212786788 2307 90-91 -0.15626071542209985 2307 92-93 -0.024504351502386612 2307 94-95 -0.05989623455520743 2307 96-97 -0.1574531879637 2307 98-99 -0.1202308630087856 2307 100-101 -0.024126578766850315 2201 1 0.06968877924036576 2201 2 0.04914789309178502 2201 3 0.08531612605298733 2201 4 0.16284395973588772 2201 5 0.11480344978762957 2201 6 0.15882789040697531 2201 7 0.24223601660366967 2201 8 0.10494508422575421 2201 9 0.2119844057002851 2201 10-11 0.2438864734539905 2201 12-13 0.256647214099452 2201 14-15 0.4700488216025178 2201 16-17 0.40747154690958354 2201 18-19 0.3800222462305243 2201 20-21 0.36192587193738746 2201 22-23 0.38453293963424073 2201 24-25 0.33731345258774326 2201 26-27 0.14679934197214806 2201 28-29 0.19754379191209637 2201 30-31 0.1540717050537168 2201 32-33 0.25539729108398745 2201 34-35 0.16127765309582998 2201 36-37 9.88477969983137E-5 2201 38-39 0.10432739213933928 2201 40-41 0.0807514393555877 2201 42-43 0.11706511525170527 2201 44-45 0.1177686446375219 2201 46-47 0.08222238903280044 2201 48-49 0.1316329453465599 2201 50-51 -0.009669374977917755 2201 52-53 0.030956591028633795 2201 54-55 0.16552690911316859 2201 56-57 0.1548101147998011 2201 58-59 0.2103218823174089 2201 60-61 0.2392907253498322 2201 62-63 0.26781970136175914 2201 64-65 0.185864155405973 2201 66-67 0.14628830332932807 2201 68-69 0.13225742832494802 2201 70-71 0.16834726818755996 2201 72-73 0.09368728931879389 2201 74-75 0.0614344061200498 2201 76-77 0.10467390556247835 2201 78-79 0.1458220969697308 2201 80-81 0.24699697737138848 2201 82-83 0.25097899014600245 2201 84-85 0.2145239832354413 2201 86-87 0.11230011315042887 2201 88-89 0.124337506391349 2201 90-91 0.1444982262641794 2201 92-93 0.22316070287418555 2201 94-95 0.20371033257778137 2201 96-97 0.1934673338002746 2201 98-99 0.15656265080165 2201 100-101 0.06057849464513154 1101 1 0.12244921102761097 1101 2 0.12211110405809222 1101 3 0.04946260925896695 1101 4 0.2547418026746229 1101 5 0.11954284721992536 1101 6 0.2322219684372584 1101 7 0.3285633539102051 1101 8 0.20258129093664934 1101 9 0.3355638126766465 1101 10-11 0.2504508255189961 1101 12-13 0.24979245342517942 1101 14-15 0.4648882358346498 1101 16-17 0.2575684768029092 1101 18-19 0.36850584525118535 1101 20-21 0.30944958330236716 1101 22-23 0.3337812881836726 1101 24-25 0.3515775603981126 1101 26-27 0.2943269622342015 1101 28-29 0.28989143855734056 1101 30-31 0.26592631446862214 1101 32-33 0.23442902735389026 1101 34-35 0.34337918744547125 1101 36-37 0.2537062197524449 1101 38-39 0.27783458972337627 1101 40-41 0.2687492831032259 1101 42-43 0.30813864380328226 1101 44-45 0.3835243871042451 1101 46-47 0.33032212033353403 1101 48-49 0.254688765846268 1101 50-51 0.1403662048693235 1101 52-53 0.16829821465262995 1101 54-55 0.38460667993022923 1101 56-57 0.4304202054960342 1101 58-59 0.48669135575764244 1101 60-61 0.4359231871409506 1101 62-63 0.43550713098933613 1101 64-65 0.39927595532822835 1101 66-67 0.40001897386837015 1101 68-69 0.38231326804184107 1101 70-71 0.4559776382044092 1101 72-73 0.18620284086113514 1101 74-75 0.24950168422363106 1101 76-77 0.3178787349954675 1101 78-79 0.381005799001052 1101 80-81 0.49559759455631536 1101 82-83 0.49644290445661454 1101 84-85 0.4807401199013306 1101 86-87 0.4676286061532444 1101 88-89 0.40852825738913623 1101 90-91 0.37835124341138027 1101 92-93 0.3793842397929481 1101 94-95 0.4896113663174049 1101 96-97 0.39852442730992976 1101 98-99 0.28894427679716017 1101 100-101 0.23883282796847638 2304 1 0.21760916974185207 2304 2 0.17312471363923265 2304 3 0.1956469685722091 2304 4 0.23664779486782805 2304 5 0.21470196288588994 2304 6 0.28659807604461207 2304 7 0.31334439361972244 2304 8 0.30933177233828957 2304 9 0.3554723132756763 2304 10-11 0.3266146093961524 2304 12-13 0.3449912345122641 2304 14-15 0.4037621568698597 2304 16-17 0.4187636688648979 2304 18-19 0.4161482944851329 2304 20-21 0.39428989352052213 2304 22-23 0.29242713599811765 2304 24-25 0.3090160998608198 2304 26-27 0.2443608190527371 2304 28-29 0.3357923186744429 2304 30-31 0.37323388216730535 2304 32-33 0.3518839666345954 2304 34-35 0.28558930340086164 2304 36-37 0.3493229209433437 2304 38-39 0.2398935184602493 2304 40-41 0.2837012078540866 2304 42-43 0.45977885322658807 2304 44-45 0.3424227911042692 2304 46-47 0.2857786533126969 2304 48-49 0.38319446615930275 2304 50-51 0.2833225534316739 2304 52-53 0.3008432964481216 2304 54-55 0.5221896074271513 2304 56-57 0.4314903077417753 2304 58-59 0.3579234885087459 2304 60-61 0.3949351286667806 2304 62-63 0.4611131204089105 2304 64-65 0.559573005915432 2304 66-67 0.6549158408227811 2304 68-69 0.4618532494796028 2304 70-71 0.4212863072613473 2304 72-73 0.4339538199141586 2304 74-75 0.5469002185167682 2304 76-77 0.5033951741416729 2304 78-79 0.4477500780965613 2304 80-81 0.34482766386246766 2304 82-83 0.33246436745392316 2304 84-85 0.4611554130667308 2304 86-87 0.4737389989403873 2304 88-89 0.35942666020889646 2304 90-91 0.4361585153265679 2304 92-93 0.46463798088287334 2304 94-95 0.42898540466278234 2304 96-97 0.3742498850998963 2304 98-99 0.38706725511471873 2304 100-101 0.3350844077355539 2202 1 0.15065883506218825 2202 2 0.08003183530551183 2202 3 0.07448186755401665 2202 4 0.17736540533595502 2202 5 0.09117870485175672 2202 6 0.12214107371113414 2202 7 0.14077442651885264 2202 8 0.2252118709340678 2202 9 0.21362455391832214 2202 10-11 0.2181566350411046 2202 12-13 0.21121216335443904 2202 14-15 0.30101490471033543 2202 16-17 0.2845418129271664 2202 18-19 0.23032055149827357 2202 20-21 0.28918352928535285 2202 22-23 0.2739154620741431 2202 24-25 0.1290991258750438 2202 26-27 0.22700258983428512 2202 28-29 0.1424341973395329 2202 30-31 0.19961972919103488 2202 32-33 0.061782614170731165 2202 34-35 0.14345760479356073 2202 36-37 0.132689268780517 2202 38-39 0.0973669217666071 2202 40-41 0.04086157697167181 2202 42-43 0.09639767980661418 2202 44-45 0.13114891489819058 2202 46-47 0.11028165743389451 2202 48-49 0.1693663973824684 2202 50-51 0.04599646311832828 2202 52-53 0.1095754569834142 2202 54-55 0.2629387463262276 2202 56-57 0.33291758334534194 2202 58-59 0.1590928559696465 2202 60-61 0.17552561457594607 2202 62-63 0.186865663522795 2202 64-65 0.2532357912958645 2202 66-67 0.2857865641704862 2202 68-69 0.3233052022978171 2202 70-71 0.2892180345510056 2202 72-73 0.1253586340415893 2202 74-75 0.2626993013079968 2202 76-77 0.1890378456591293 2202 78-79 0.20421281430288474 2202 80-81 0.11614463465452474 2202 82-83 0.18521868705525435 2202 84-85 0.1524047625755678 2202 86-87 0.17958284499791333 2202 88-89 0.10248563010289402 2202 90-91 0.10786732419367695 2202 92-93 0.1001011088164816 2202 94-95 0.06781120230763449 2202 96-97 -0.017651777827140336 2202 98-99 -0.0558121138918537 2202 100-101 0.0462253014284677 1102 1 0.06443868018998344 1102 2 -0.024605131814190884 1102 3 0.09813162428723032 1102 4 0.0532079889821091 1102 5 0.022488166805729293 1102 6 0.059502511105883116 1102 7 0.1412906296072336 1102 8 0.2574957816458081 1102 9 0.19367848209130756 1102 10-11 0.1258058195311591 1102 12-13 0.10802892507422968 1102 14-15 0.16217005486001312 1102 16-17 0.1307021550662668 1102 18-19 0.18326181649683804 1102 20-21 0.14341427636578885 1102 22-23 0.12475167865161296 1102 24-25 0.04925074493619519 1102 26-27 0.12992939367435952 1102 28-29 0.12772607483494625 1102 30-31 0.1971824089554346 1102 32-33 0.07804567247701044 1102 34-35 0.07296391571643568 1102 36-37 0.07135180511756545 1102 38-39 0.07269732381642058 1102 40-41 0.04814995855339532 1102 42-43 0.03147114904734849 1102 44-45 0.09268108785660445 1102 46-47 0.11586680849220699 1102 48-49 0.12742302844923614 1102 50-51 0.19592462961331591 1102 52-53 0.1657516651598101 1102 54-55 0.24123537764111802 1102 56-57 0.3110070954844275 1102 58-59 0.31828634979469683 1102 60-61 0.2829028884019138 1102 62-63 0.43037068954194524 1102 64-65 0.27414471264287954 1102 66-67 0.27073848284513247 1102 68-69 0.31019808123990344 1102 70-71 0.25010197541864443 1102 72-73 0.3235643190144728 1102 74-75 0.3710987954106386 1102 76-77 0.3854926836691277 1102 78-79 0.2915331521194915 1102 80-81 0.22344928548010756 1102 82-83 0.2107290270831612 1102 84-85 0.3053944088372269 1102 86-87 0.2889379809507737 1102 88-89 0.22419358420529534 1102 90-91 0.11913687715672694 1102 92-93 0.16176096150369546 1102 94-95 0.1923254599948372 1102 96-97 0.19009041203704768 1102 98-99 0.2693044054035205 1102 100-101 0.1552005632254847 2305 1 0.09327985052019727 2305 2 9.990880622616771E-4 2305 3 0.041427450251742215 2305 4 -0.009693735178096574 2305 5 0.008017876881773134 2305 6 0.21887906118109157 2305 7 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0.2019913366769046 1213 48-49 0.26132194449137813 1213 50-51 0.1926877956992854 1213 52-53 0.1725574090045754 1213 54-55 -0.007853652724470805 1213 56-57 -0.016364664098595938 1213 58-59 0.0814533085571938 1213 60-61 0.1705081797890884 1213 62-63 0.1593342779510678 1213 64-65 0.19903468978112926 1213 66-67 0.255458300539118 1213 68-69 0.24764210743629178 1213 70-71 0.2746144819462373 1213 72-73 0.3294225276543088 1213 74-75 0.34156451766885 1213 76-77 0.29868399804152546 1213 78-79 0.30639037275394543 1213 80-81 0.27552336640724917 1213 82-83 0.3324855782920437 1213 84-85 0.40240918371642564 1213 86-87 0.38004801336735383 1213 88-89 0.21107589249605496 1213 90-91 0.30007358693157116 1213 92-93 0.26528321358269835 1213 94-95 0.36325174766334456 1213 96-97 0.35555347731964204 1213 98-99 0.16737355953346622 1213 100-101 0.23434106744081618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2918.0 3 709.0 4 714.0 5 702.0 6 1975.0 7 7626.0 8 31627.0 9 74430.0 10 84491.0 11 54811.0 12 32041.0 13 22296.0 14 17462.0 15 15382.0 16 15095.0 17 15612.0 18 16924.0 19 18443.0 20 20264.0 21 22761.0 22 25350.0 23 28743.0 24 32659.0 25 37422.0 26 44027.0 27 52153.0 28 63236.0 29 77514.0 30 95316.0 31 120380.0 32 159755.0 33 224005.0 34 334272.0 35 542655.0 36 977710.0 37 1742675.0 38 916753.0 39 6704.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.35908930356869 26.321654606515065 20.787633728829277 16.53162236108697 2 31.009679811487118 26.071349619078134 28.874960249391474 14.044010320043274 3 34.951411234397256 22.951601000628557 25.672382783475552 16.424604981498636 4 29.338408251226326 22.222556093463393 30.69038420439402 17.748651450916256 5 31.519190654562816 20.31158097607389 30.632335731278836 17.536892638084453 6 28.8664582779669 20.687446479116836 33.30717928599451 17.138915956921753 7 33.21183347553017 21.143809073676177 26.991894274376133 18.652463176417523 8 28.6519595399113 29.518467459524818 24.153239878694627 17.67633312186926 9 29.99534379913274 27.598555549016 24.123117303713983 18.282983348137275 10-11 26.612470465740255 29.807519880956647 27.677385767490286 15.902623885812805 12-13 27.80682708585819 27.429974995707816 26.97184124753642 17.791356670897574 14-15 25.932598505089903 27.59948836147154 28.048909243191222 18.41900389024733 16-17 26.02690714700222 27.794638639484738 28.178190451022054 18.000263762490984 18-19 25.386403138794357 28.39621587600295 28.561047265295485 17.656333719907206 20-21 24.382053972653214 29.02348629562066 28.609334231036616 17.98512550068951 22-23 25.68295806885677 28.56344375941577 27.60896653784214 18.144631633885318 24-25 23.470525582257252 30.2270099473852 28.169922329564262 18.13254214079329 26-27 22.559972276210242 30.999329186918505 27.875684865720913 18.565013671150346 28-29 23.05039805479623 30.796112123781032 27.749470552652834 18.404019268769908 30-31 24.302475523200897 28.785621189179217 28.334283684997235 18.57761960262265 32-33 24.430664597661874 28.88440694640815 28.00094832202596 18.683980133904015 34-35 22.629770527308764 28.804123265333853 28.279766834776858 20.286339372580525 36-37 21.303939227751204 28.54981375567695 28.2856743825532 21.860572634018645 38-39 20.80283021610064 29.70293850639163 29.180017061466568 20.314214216041158 40-41 21.285121897758287 28.690500071570856 30.75654028340707 19.267837747263787 42-43 22.18211992474312 28.634941850682715 29.96546712176408 19.217471102810084 44-45 21.377864474571272 29.294995603433875 30.254982246862856 19.072157675131997 46-47 22.572976367955395 29.688449539142216 28.254701685779388 19.483872407123005 48-49 20.87466448260118 29.934468739788073 29.342239281759742 19.848627495851005 50-51 20.44170987958975 28.530290174496827 30.500326209680146 20.527673736233275 52-53 22.85146899198409 28.589495276353954 28.36927871683253 20.189757014829425 54-55 23.423484469749553 28.52704327090636 28.75348639697902 19.29598586236506 56-57 23.99684722084444 28.22938901891211 28.169336484985852 19.604427275257596 58-59 23.044001997037565 28.850053284470295 28.15600753358679 19.94993718490535 60-61 21.379753269212916 28.344770978338264 28.353691217964954 21.921784534483866 62-63 22.365966038031935 28.582433740310613 28.05248798320863 20.999112238448816 64-65 21.12276597410713 29.198933078707533 29.629669987428358 20.048630959756977 66-67 20.57984681254572 28.98838679219699 28.83063046181554 21.601135933441746 68-69 20.205510521593354 28.92867563096025 30.877937285535857 19.987876561910543 70-71 20.046484386051812 29.951121296461693 30.14199949579981 19.86039482168669 72-73 20.04007604770818 31.990404854730027 28.07394957245122 19.895569525110574 74-75 19.842778260194372 32.141527432737085 28.396560682607607 19.619133624460932 76-77 20.142080459283818 31.918313362874596 27.94833124622469 19.991274931616896 78-79 19.935729581157027 32.259274132761064 28.06840365707973 19.736592629002175 80-81 19.857953016956934 31.704807895425663 28.380662197263508 20.056576890353895 82-83 20.04518967370758 31.765028903565373 27.915661149257293 20.27412027346976 84-85 19.87500379082294 31.424718395708528 28.484813773236056 20.21546404023248 86-87 20.22300384015144 30.91350628685622 28.275714729580116 20.58777514341223 88-89 20.17563186633548 31.3182113790093 28.237341848612957 20.268814906042266 90-91 20.250481005438218 30.734203186699045 28.4580607861119 20.55725502175084 92-93 20.343145079895166 30.87331892441568 28.051088312475876 20.732447683213273 94-95 20.085148898667995 30.78528787160269 28.5672612534928 20.562301976236515 96-97 20.388290794726018 30.522799472628943 28.176069220492327 20.912840512152712 98-99 20.305911477116574 30.822077854275783 28.15029652761692 20.721714140990727 100-101 20.43814729972801 30.43639689346818 28.292309735401428 20.833146071402382 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1672.0 1 1336.5 2 767.0 3 546.0 4 593.5 5 717.5 6 952.5 7 1253.0 8 1572.5 9 1947.5 10 2269.0 11 2425.0 12 2415.5 13 2280.5 14 2079.5 15 1911.5 16 1885.5 17 2033.5 18 2378.0 19 3053.0 20 4131.5 21 5898.0 22 8512.5 23 12022.5 24 16655.5 25 22644.5 26 30354.5 27 39742.5 28 50586.5 29 63039.0 30 77274.0 31 93405.0 32 112458.0 33 134428.0 34 157723.5 35 183413.0 36 212865.0 37 242793.5 38 269198.5 39 289062.0 40 302339.5 41 306070.5 42 299889.5 43 289650.0 44 277678.0 45 266481.0 46 256957.0 47 245166.0 48 228171.5 49 209020.5 50 191143.5 51 172420.5 52 151763.0 53 130663.5 54 109795.0 55 90961.0 56 74717.0 57 61039.5 58 49458.0 59 39277.5 60 30693.0 61 23623.5 62 17787.0 63 13278.0 64 9846.0 65 7197.0 66 5346.5 67 3983.5 68 2947.0 69 2202.5 70 1684.0 71 1295.5 72 1009.5 73 779.0 74 618.0 75 525.5 76 442.0 77 373.5 78 310.0 79 260.0 80 210.0 81 176.0 82 156.5 83 141.5 84 116.0 85 87.5 86 71.0 87 60.5 88 50.0 89 44.5 90 40.5 91 32.5 92 24.0 93 17.5 94 18.5 95 14.5 96 11.0 97 8.5 98 3.5 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2981501654200375 2 0.2748411314178158 3 0.24479538238605017 4 0.23305665644707 5 0.23928811784939805 6 0.188409077588768 7 0.15511286355524748 8 0.15331079228484448 9 0.13279749501988342 10-11 0.11788409212323069 12-13 0.138607911732865 14-15 0.1419089020973415 16-17 0.13532376315596237 18-19 0.12310504627112719 20-21 0.12908388085984737 22-23 0.1581949106812638 24-25 0.5717786881325354 26-27 0.2266567771690033 28-29 0.19970149615704094 30-31 0.3010216902013806 32-33 0.3333158178742565 34-35 0.3412651416091183 36-37 0.3320105793372824 38-39 0.33993464039078336 40-41 0.3435556247191632 42-43 0.3549659358004531 44-45 0.3355810383029406 46-47 0.3552017208264871 48-49 0.35060391281882347 50-51 0.3565743265137567 52-53 0.3411304073085274 54-55 0.3333073969804696 56-57 0.34177039523633407 58-59 0.3342505370846057 60-61 0.3113793895593043 62-63 0.2975354401735917 64-65 0.30883627963565147 66-67 0.3251728135822954 68-69 0.31155622832882984 70-71 0.29211238457480887 72-73 0.28829771968932966 74-75 0.27976735428316973 76-77 0.2819567866677715 78-79 0.29160713094759305 80-81 0.3011395827143976 82-83 0.32377494521366507 84-85 0.3151519499758489 86-87 0.3172150689536467 88-89 0.32257075740213403 90-91 0.3159519348856072 92-93 0.2975438610673786 94-95 0.2929544739535018 96-97 0.31056256286197215 98-99 0.3189666148613281 100-101 0.3197581788772995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5937612.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.65300384272763 #Duplication Level Percentage of deduplicated Percentage of total 1 97.0018800066714 88.90513681003257 2 2.069321537470718 3.7931906965128555 3 0.3587434072412788 0.9863973264731438 4 0.1669966720857699 0.6122298651359919 5 0.08730623729212603 0.4000939451014658 6 0.06597897256482585 0.3628302615613923 7 0.033174403242536614 0.21283735955078828 8 0.04442515684401645 0.32573592567507337 9 0.028876862497939505 0.238198607054061 >10 0.12253431191830645 2.088814384040432 >50 0.013188825773697633 0.8507980396481609 >100 0.007472528938461274 1.1694578864945373 >500 1.0107745887071932E-4 0.054278892719536065 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001532602669221229 0.0 0.0 2 0.0017852294828291238 0.0 0.0 3 0.00198733093371544 0.0 0.0 4 0.0021389070218801767 0.0 0.0 5 0.0025936352863743877 0.0 0.0 6 0.003098888913590177 0.0 0.0 7 0.0037894022041184234 3.3683575147719316E-5 0.0 8 0.004682016945532985 3.3683575147719316E-5 0.0 9 0.005322004873339652 3.3683575147719316E-5 0.0 10-11 0.0072167059753988645 3.3683575147719316E-5 0.0 12-13 0.00943140104136141 3.3683575147719316E-5 2.526268136078949E-5 14-15 0.011460836444011498 3.3683575147719316E-5 3.3683575147719316E-5 16-17 0.013540797209383167 3.3683575147719316E-5 3.3683575147719316E-5 18-19 0.015999698195166678 3.3683575147719316E-5 5.8946256508508804E-5 20-21 0.01881227672000124 3.3683575147719316E-5 6.736715029543863E-5 22-23 0.02181853580193519 3.3683575147719316E-5 6.736715029543863E-5 24-25 0.024959529184460018 3.3683575147719316E-5 8.42089378692983E-5 26-27 0.02815104792970642 3.3683575147719316E-5 1.0105072544315795E-4 28-29 0.03220149784121967 3.3683575147719316E-5 1.1789251301701762E-4 30-31 0.04795699011656538 3.3683575147719316E-5 1.431551943778071E-4 32-33 0.053026368176297134 3.3683575147719316E-5 1.684178757385966E-4 34-35 0.0576747015466824 5.052536272157898E-5 1.7683876952552643E-4 36-37 0.06281986765049653 5.052536272157898E-5 2.1052234467324574E-4 38-39 0.06889133207087295 5.052536272157898E-5 2.3578502603403525E-4 40-41 0.07547647101225206 5.052536272157898E-5 2.3578502603403525E-4 42-43 0.08276054413794637 5.052536272157898E-5 2.442059198209651E-4 44-45 0.08998567100713216 5.052536272157898E-5 2.526268136078949E-4 46-47 0.09824656781211033 5.052536272157898E-5 2.610477073948247E-4 48-49 0.10723166148276445 5.052536272157898E-5 2.694686011817545E-4 50-51 0.11606517906525385 5.052536272157898E-5 2.863103887556142E-4 52-53 0.12640603663560368 5.052536272157898E-5 2.863103887556142E-4 54-55 0.13726056872695624 5.052536272157898E-5 3.0315217632947386E-4 56-57 0.14903297824108414 5.052536272157898E-5 3.0315217632947386E-4 58-59 0.1614790592581664 5.052536272157898E-5 3.1999396390333353E-4 60-61 0.1753903757941745 5.052536272157898E-5 3.284148576902634E-4 62-63 0.1906069308671567 5.052536272157898E-5 3.620984328379827E-4 64-65 0.2067582051504881 6.736715029543863E-5 4.042029017726318E-4 66-67 0.22610099817906593 6.736715029543863E-5 4.126237955595617E-4 68-69 0.24640377309935374 6.736715029543863E-5 4.46307370707281E-4 70-71 0.2687612461036525 6.736715029543863E-5 4.799909458550003E-4 72-73 0.2928871068032064 6.736715029543863E-5 4.884118396419301E-4 74-75 0.3199939639033335 6.736715029543863E-5 5.136745210027197E-4 76-77 0.3495176175203095 8.42089378692983E-5 5.220954147896494E-4 78-79 0.3821485809446626 9.262983165622812E-5 5.220954147896494E-4 80-81 0.4200510238796338 1.0105072544315795E-4 5.305163085765792E-4 82-83 0.46165023918706716 1.0105072544315795E-4 5.557789899373688E-4 84-85 0.5089756622696128 1.0105072544315795E-4 5.726207775112284E-4 86-87 0.5615220395000549 1.0105072544315795E-4 5.894625650850881E-4 88-89 0.6189609560207032 1.0105072544315795E-4 5.978834588720179E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 3500 0.0 79.48079 2 GAGCGGC 2510 0.0 78.75852 9 GATCGGG 3850 0.0 73.87317 1 AGAGCGG 3025 0.0 68.9568 8 AAGCGGC 1075 0.0 63.06128 9 GAGAGCG 3590 0.0 62.99084 7 GATCCGG 905 0.0 62.433697 1 TCGGGAG 4070 0.0 61.45187 3 GGTTCGG 1075 0.0 61.39415 1 GTTCGGA 875 0.0 61.306 2 TCGGAGA 1210 0.0 61.183758 4 CGGGAGA 4460 0.0 59.693214 4 GGATCGG 1705 0.0 59.316547 1 TTCGGAA 985 0.0 57.3363 3 TTCGGAG 895 0.0 54.617664 3 TCGGAAA 1990 0.0 53.18005 4 TCGGGAA 1475 0.0 52.44626 3 CGGAAAG 2810 0.0 50.834328 5 ATCGGAG 990 0.0 50.81473 3 CCGGAAA 1165 0.0 49.697075 4 >>END_MODULE