##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_35008-1.342000000357b1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2463384 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79349463989374 33.0 33.0 33.0 27.0 33.0 2 31.722577154028766 33.0 33.0 33.0 27.0 33.0 3 31.67095061102938 33.0 33.0 33.0 27.0 33.0 4 35.364551365114 37.0 37.0 37.0 33.0 37.0 5 35.380744130837904 37.0 37.0 37.0 33.0 37.0 6 35.443844321469975 37.0 37.0 37.0 33.0 37.0 7 35.36423066805663 37.0 37.0 37.0 33.0 37.0 8 35.32200744991442 37.0 37.0 37.0 33.0 37.0 9 35.20799436872205 37.0 37.0 37.0 33.0 37.0 10-11 35.17347985535345 37.0 37.0 37.0 33.0 37.0 12-13 35.148482331621864 37.0 37.0 37.0 33.0 37.0 14-15 36.815713668676906 40.0 37.0 40.0 33.0 40.0 16-17 36.78488372093023 40.0 37.0 40.0 33.0 40.0 18-19 36.79427750606483 40.0 37.0 40.0 33.0 40.0 20-21 36.798117345894916 40.0 37.0 40.0 33.0 40.0 22-23 36.857537030361485 40.0 37.0 40.0 33.0 40.0 24-25 36.55392196263352 40.0 37.0 40.0 33.0 40.0 26-27 36.46593568034866 40.0 37.0 40.0 33.0 40.0 28-29 36.44582939566061 40.0 37.0 40.0 33.0 40.0 30-31 36.45271484267171 40.0 37.0 40.0 33.0 40.0 32-33 36.341387091902845 40.0 37.0 40.0 33.0 40.0 34-35 36.274467764668444 40.0 37.0 40.0 33.0 40.0 36-37 36.19135059738961 40.0 37.0 40.0 33.0 40.0 38-39 36.127135882996726 40.0 37.0 40.0 33.0 40.0 40-41 36.05195657680654 38.5 37.0 40.0 30.0 40.0 42-43 35.912983521854486 37.0 37.0 40.0 27.0 40.0 44-45 35.80089807354436 37.0 37.0 40.0 27.0 40.0 46-47 35.713549531863485 37.0 37.0 40.0 27.0 40.0 48-49 35.59286493701347 37.0 37.0 40.0 27.0 40.0 50-51 34.43980820692186 37.0 35.0 38.5 27.0 38.5 52-53 34.883972819503576 37.0 35.0 38.5 27.0 40.0 54-55 35.64760082065971 37.0 37.0 40.0 27.0 40.0 56-57 35.72841607317414 37.0 37.0 40.0 27.0 40.0 58-59 35.50385425090039 37.0 37.0 40.0 27.0 40.0 60-61 35.23461222448469 37.0 37.0 40.0 27.0 40.0 62-63 34.99431128074227 37.0 35.0 40.0 27.0 40.0 64-65 34.75477716831806 37.0 33.0 40.0 27.0 40.0 66-67 34.46068700616713 37.0 33.0 37.0 27.0 40.0 68-69 34.25919730744374 37.0 33.0 37.0 27.0 40.0 70-71 33.95690501359106 37.0 33.0 37.0 27.0 40.0 72-73 33.732727621840525 37.0 33.0 37.0 27.0 38.5 74-75 33.50362428269405 37.0 33.0 37.0 27.0 37.0 76-77 33.26296833948747 37.0 33.0 37.0 27.0 37.0 78-79 33.02052359680829 37.0 33.0 37.0 27.0 37.0 80-81 32.79323463170988 37.0 33.0 37.0 24.5 37.0 82-83 32.61527394835721 37.0 33.0 37.0 22.0 37.0 84-85 32.36410157734239 35.0 33.0 37.0 22.0 37.0 86-87 32.24074525124787 33.0 33.0 37.0 22.0 37.0 88-89 32.15102537809777 33.0 33.0 37.0 22.0 37.0 90-91 32.01116208435226 33.0 33.0 37.0 22.0 37.0 92-93 31.83902773583006 33.0 33.0 37.0 22.0 37.0 94-95 31.700477879210062 33.0 33.0 37.0 22.0 37.0 96-97 31.556924133630808 33.0 33.0 37.0 22.0 37.0 98-99 31.41171291199423 33.0 33.0 37.0 15.0 37.0 100-101 30.134941811751638 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11977148258188208 2306 2 0.24543227226403275 2306 3 0.24868525814045483 2306 4 0.36740393103418967 2306 5 0.22408655939619848 2306 6 0.1582089691242956 2306 7 0.2749434412208629 2306 8 0.40968962257358044 2306 9 0.4144252558771484 2306 10-11 0.27954017404387344 2306 12-13 0.31490071720911317 2306 14-15 0.4701331741655821 2306 16-17 0.4331372189741245 2306 18-19 0.44256536181622863 2306 20-21 0.4732593695769367 2306 22-23 0.41098251049135825 2306 24-25 0.36266243652424635 2306 26-27 0.3553450080991851 2306 28-29 0.2822533048878668 2306 30-31 0.22352130240181367 2306 32-33 0.32299501512193274 2306 34-35 0.3258151641251388 2306 36-37 0.19644608447826784 2306 38-39 0.3472251237155817 2306 40-41 0.24074067952629008 2306 42-43 0.30244190659137615 2306 44-45 0.41334387178263654 2306 46-47 0.39881924831995974 2306 48-49 0.3413541536960736 2306 50-51 0.23367509703781053 2306 52-53 0.417415736318695 2306 54-55 0.578881137257298 2306 56-57 0.4276146415764117 2306 58-59 0.4433702458145632 2306 60-61 0.47472659239136306 2306 62-63 0.5333949396324371 2306 64-65 0.48864980847945816 2306 66-67 0.43414245063483037 2306 68-69 0.40401177384334375 2306 70-71 0.48685548456683847 2306 72-73 0.4531509518360721 2306 74-75 0.3937760298690307 2306 76-77 0.3616837938209443 2306 78-79 0.42280122047774427 2306 80-81 0.49982142609142244 2306 82-83 0.5085735574002328 2306 84-85 0.5572293175488809 2306 86-87 0.40321940622505537 2306 88-89 0.4089396442250859 2306 90-91 0.33494608136710013 2306 92-93 0.32351793837342413 2306 94-95 0.3183200706995315 2306 96-97 0.22995744515813854 2306 98-99 0.34826788984684853 2306 100-101 0.2524574513944309 2307 1 0.09853972558816793 2307 2 0.09426903613681148 2307 3 0.05233763613824394 2307 4 0.17526278883990898 2307 5 0.21870173649835323 2307 6 0.10588897128243246 2307 7 0.23147126770660975 2307 8 0.26672649974857876 2307 9 0.3279635223531798 2307 10-11 0.23040855463142407 2307 12-13 0.1640694296103078 2307 14-15 0.28829336192355015 2307 16-17 0.2769938107354335 2307 18-19 0.29707222739123296 2307 20-21 0.26458756834140473 2307 22-23 0.3688575543285211 2307 24-25 0.19591083815363675 2307 26-27 0.17364803758474778 2307 28-29 0.09093265960474639 2307 30-31 0.04126483473341125 2307 32-33 0.17569390098342552 2307 34-35 -0.00426455207977483 2307 36-37 0.18688270267676188 2307 38-39 0.15182546362223803 2307 40-41 0.1399193733135462 2307 42-43 0.13476082536448075 2307 44-45 0.17406981529339305 2307 46-47 0.07780735158683427 2307 48-49 0.05440953690037276 2307 50-51 -0.09497336933571177 2307 52-53 -0.053590899954194526 2307 54-55 -0.010031161428941004 2307 56-57 -0.07571455532665539 2307 58-59 0.0029989112762720538 2307 60-61 0.024476518205389652 2307 62-63 0.06022018444615895 2307 64-65 0.10238527477203974 2307 66-67 0.1822424803092204 2307 68-69 0.042134733728957485 2307 70-71 -0.04556297829970646 2307 72-73 -0.044288957522148564 2307 74-75 0.002210975510941182 2307 76-77 -0.002632346350090131 2307 78-79 -0.02890492212118545 2307 80-81 0.12560901140529523 2307 82-83 0.09468553776118682 2307 84-85 0.14616481621352762 2307 86-87 0.11479106987932397 2307 88-89 0.10528052204752925 2307 90-91 0.19463045375373866 2307 92-93 0.07283664133277412 2307 94-95 0.017999317509538315 2307 96-97 0.15435114339655698 2307 98-99 0.04730468609168881 2307 100-101 -0.05060548529120723 2201 1 0.10145183502316257 2201 2 0.08405926321838919 2201 3 0.05097690851453507 2201 4 0.09316394771780523 2201 5 0.05576221957189631 2201 6 0.05130221486867015 2201 7 0.1371360326548725 2201 8 0.006115680288445446 2201 9 0.12784643884943847 2201 10-11 0.01678789959846938 2201 12-13 0.0798994138020035 2201 14-15 0.20417868914172743 2201 16-17 0.13406993709930504 2201 18-19 0.25427087001465765 2201 20-21 0.27285805052894574 2201 22-23 0.37989442623912595 2201 24-25 0.28996529619944056 2201 26-27 0.21626039090985927 2201 28-29 0.18230843900845883 2201 30-31 0.10686675742123697 2201 32-33 0.14795930176722294 2201 34-35 0.15462502309648585 2201 36-37 0.10174456470559079 2201 38-39 0.06530781186239665 2201 40-41 0.22201580269825882 2201 42-43 0.19768368860957963 2201 44-45 0.25843784743499043 2201 46-47 0.23420649057120357 2201 48-49 0.2552054610133965 2201 50-51 0.1414921508163829 2201 52-53 0.2008831902432533 2201 54-55 0.40961000251107293 2201 56-57 0.3171693977089518 2201 58-59 0.3726832199289021 2201 60-61 0.37161197077044505 2201 62-63 0.21762019444852854 2201 64-65 0.21025873421131536 2201 66-67 0.3015358971038964 2201 68-69 0.27099960002097134 2201 70-71 0.3289821236012713 2201 72-73 0.27673740682942594 2201 74-75 0.27951089079549263 2201 76-77 0.17702711125271264 2201 78-79 0.23203833629850124 2201 80-81 0.21467713892476326 2201 82-83 0.16319322320664753 2201 84-85 0.21780255599418297 2201 86-87 0.18511377811316976 2201 88-89 0.1011019966142328 2201 90-91 0.16327850231546392 2201 92-93 0.2326223673507748 2201 94-95 0.010084883921297916 2201 96-97 0.11702112962934663 2201 98-99 0.1571246714740262 2201 100-101 -0.015485511510600958 1101 1 0.16933718357788763 1101 2 0.18889402462765048 1101 3 0.11297010570605792 1101 4 0.20328137681001834 1101 5 0.10256828243578298 1101 6 0.07777264777336512 1101 7 0.14322444632729514 1101 8 0.11242990266863018 1101 9 0.2692687767831572 1101 10-11 0.23429471168479665 1101 12-13 0.2607768990180972 1101 14-15 0.3464959832054717 1101 16-17 0.2772001139637652 1101 18-19 0.32416023516675097 1101 20-21 0.34092431724871375 1101 22-23 0.30062657743103927 1101 24-25 0.2851729274489685 1101 26-27 0.21400444487556314 1101 28-29 0.22321771158183168 1101 30-31 0.1352248769000184 1101 32-33 0.17225407776412993 1101 34-35 0.143539771031449 1101 36-37 0.17503676145890523 1101 38-39 0.1617850104641505 1101 40-41 0.059101314543219985 1101 42-43 0.07622622329090945 1101 44-45 0.16702707113548598 1101 46-47 0.17230678560698465 1101 48-49 0.12852741826960568 1101 50-51 0.06439353578597462 1101 52-53 0.15362358302480317 1101 54-55 0.3211335261546111 1101 56-57 0.32004803052429054 1101 58-59 0.3803046206565739 1101 60-61 0.25034113306581673 1101 62-63 0.21587560093988145 1101 64-65 0.20689693927474195 1101 66-67 0.3388706977656213 1101 68-69 0.18088522548397634 1101 70-71 0.2273368031370424 1101 72-73 0.0063336203228487875 1101 74-75 0.1633207490156039 1101 76-77 0.10109251013622611 1101 78-79 0.21620103846652228 1101 80-81 0.11043723073270684 1101 82-83 -0.06602652349364035 1101 84-85 0.10246669052021673 1101 86-87 0.219198474934295 1101 88-89 0.2827987877611591 1101 90-91 0.1709205997957426 1101 92-93 -0.013162631928409496 1101 94-95 0.06282484849416292 1101 96-97 0.08518773991520234 1101 98-99 0.20553544634616472 1101 100-101 0.030967992877823036 2304 1 0.16237977321998187 2304 2 0.2563265433399522 2304 3 0.22594189530859765 2304 4 0.31186597109008574 2304 5 0.24764035539060814 2304 6 0.36344883870976474 2304 7 0.3179755790876584 2304 8 0.3195638657035289 2304 9 0.34076433366009695 2304 10-11 0.2761028195957991 2304 12-13 0.36501948758638036 2304 14-15 0.534846727961579 2304 16-17 0.5691223144561945 2304 18-19 0.530150364145058 2304 20-21 0.5114219219756322 2304 22-23 0.2936606660572565 2304 24-25 0.3944067308884769 2304 26-27 0.32647914876057627 2304 28-29 0.4026096160196744 2304 30-31 0.45496284874554505 2304 32-33 0.36597824753925323 2304 34-35 0.3248021226719402 2304 36-37 0.4429225632859115 2304 38-39 0.2899312252526087 2304 40-41 0.42344445223238125 2304 42-43 0.42400222331238524 2304 44-45 0.23544447727867635 2304 46-47 0.3884932119901947 2304 48-49 0.37337557894060325 2304 50-51 0.3523476634094962 2304 52-53 0.2842368821035137 2304 54-55 0.4649687013001511 2304 56-57 0.3615550235046925 2304 58-59 0.35679595610800163 2304 60-61 0.4876935229922026 2304 62-63 0.438730291285907 2304 64-65 0.4689851601329238 2304 66-67 0.5213152498896036 2304 68-69 0.4669088395163783 2304 70-71 0.5055513392477948 2304 72-73 0.4201747059115277 2304 74-75 0.4495398864130493 2304 76-77 0.36396371929835425 2304 78-79 0.5746014067842253 2304 80-81 0.44027787695308973 2304 82-83 0.4267640557700503 2304 84-85 0.5387151116802258 2304 86-87 0.44455615517708935 2304 88-89 0.4739512883797232 2304 90-91 0.6031435662297042 2304 92-93 0.558117379454 2304 94-95 0.48917708048061925 2304 96-97 0.5066458475801277 2304 98-99 0.571130023055975 2304 100-101 0.4778323931736601 2202 1 0.03328072739201815 2202 2 0.10909567605653336 2202 3 0.04280120839344903 2202 4 0.09047387003263196 2202 5 0.028317419565489388 2202 6 0.06564974086496989 2202 7 0.1318446565232705 2202 8 0.1667473612599153 2202 9 0.1523715366734777 2202 10-11 0.12977283115834837 2202 12-13 0.09453163412403853 2202 14-15 0.11497357705960809 2202 16-17 0.116802041407567 2202 18-19 0.29635289669115394 2202 20-21 0.2555894873241371 2202 22-23 0.20727087290682533 2202 24-25 0.22473057857749268 2202 26-27 0.26172771013730056 2202 28-29 0.17165392908987798 2202 30-31 0.05077363133735702 2202 32-33 0.09093065611119755 2202 34-35 0.16474385377378553 2202 36-37 0.20222397259478697 2202 38-39 -0.040974620929056016 2202 40-41 0.10005949543397463 2202 42-43 0.0742648602018221 2202 44-45 0.2085535942018879 2202 46-47 0.15689746272389726 2202 48-49 0.14112480674378247 2202 50-51 -0.08013820981422981 2202 52-53 0.07265359511664116 2202 54-55 0.2624026769556025 2202 56-57 0.34536514992275613 2202 58-59 0.2307218796160555 2202 60-61 0.3353730197805902 2202 62-63 0.27736595206918935 2202 64-65 0.2692702274514076 2202 66-67 0.2989890009729379 2202 68-69 0.23408412453137828 2202 70-71 0.17724371930462723 2202 72-73 0.21759508102697822 2202 74-75 0.06301871204617271 2202 76-77 0.1698903952301265 2202 78-79 0.22213043975234825 2202 80-81 0.24082062219488876 2202 82-83 0.27932614641995457 2202 84-85 0.4069006657306602 2202 86-87 0.3375126865957938 2202 88-89 0.25998612835994805 2202 90-91 0.18679598915544204 2202 92-93 0.10140298116909463 2202 94-95 0.15717806474124174 2202 96-97 0.11831276742607244 2202 98-99 -0.014278334203840615 2202 100-101 0.17123458290244642 1102 1 -0.00216093316644006 1102 2 0.07368367836399159 1102 3 0.12316923088726739 1102 4 0.13657581818038267 1102 5 0.18315601844781781 1102 6 0.11310173971280335 1102 7 0.08883662335082221 1102 8 0.26208342201571355 1102 9 0.16875558648211353 1102 10-11 0.23417870346908387 1102 12-13 0.10738482713640707 1102 14-15 0.16450100076944807 1102 16-17 0.18336219496753614 1102 18-19 0.32349110144766513 1102 20-21 0.2112901437321355 1102 22-23 0.12271921541613295 1102 24-25 0.1861653401778227 1102 26-27 0.10482715177288071 1102 28-29 0.11307250101022959 1102 30-31 0.1488921469763298 1102 32-33 0.1508424246971245 1102 34-35 0.17575228973673518 1102 36-37 0.21136423345627264 1102 38-39 0.17836646534070866 1102 40-41 0.16557286206778343 1102 42-43 0.2079579338445683 1102 44-45 0.1968090508031679 1102 46-47 0.13757776494166052 1102 48-49 0.2324442812739207 1102 50-51 0.3552092321148592 1102 52-53 0.39442680221105775 1102 54-55 0.33168693541907857 1102 56-57 0.3536670445212238 1102 58-59 0.36632625660610074 1102 60-61 0.35657669984941265 1102 62-63 0.40920704809646935 1102 64-65 0.3178857131847437 1102 66-67 0.42911570044048375 1102 68-69 0.3953501980968497 1102 70-71 0.38039336924378375 1102 72-73 0.2701590454846112 1102 74-75 0.3544772507753393 1102 76-77 0.4117393519168786 1102 78-79 0.4109101875087049 1102 80-81 0.3283375905250878 1102 82-83 0.38326307223858436 1102 84-85 0.46073427890971175 1102 86-87 0.4187436643072786 1102 88-89 0.2480228213250868 1102 90-91 0.2909747063613892 1102 92-93 0.35677254535134395 1102 94-95 0.28484391724029834 1102 96-97 0.4499309235979432 1102 98-99 0.4263965194138173 1102 100-101 0.34052777925579036 2305 1 0.10927086234441674 2305 2 0.12790347552471104 2305 3 0.03248465562406366 2305 4 0.07036600794364034 2305 5 0.08755988918531443 2305 6 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0.22674428470637054 1213 86-87 0.07878474860609685 1213 88-89 -0.0064162749472487235 1213 90-91 0.02061672033352835 1213 92-93 -0.04734323727130629 1213 94-95 0.06025301515976267 1213 96-97 0.21859460649590545 1213 98-99 0.08096339886391135 1213 100-101 0.16725479725193182 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1152.0 3 254.0 4 254.0 5 280.0 6 776.0 7 3469.0 8 13032.0 9 17154.0 10 15615.0 11 12496.0 12 9054.0 13 6748.0 14 5424.0 15 4956.0 16 4794.0 17 5235.0 18 5667.0 19 6337.0 20 7265.0 21 8245.0 22 9588.0 23 10697.0 24 12552.0 25 14524.0 26 17080.0 27 20482.0 28 24906.0 29 30818.0 30 38948.0 31 50552.0 32 69905.0 33 100229.0 34 152562.0 35 257586.0 36 499919.0 37 819327.0 38 205373.0 39 129.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.04130396455682 24.91816935513095 26.289919287032053 14.750607393280177 2 29.516683105462988 22.59394843333454 35.06075705847177 12.828611402730699 3 38.317751304470974 19.867961737145944 25.970834869561045 15.84345208882204 4 28.14187349905966 18.711227827179563 36.550150429723644 16.596748244037133 5 31.58842010899841 17.669779492461142 34.37954609359963 16.36225430494082 6 24.335110763695848 18.47308821848769 41.17093224195774 16.020868775858723 7 32.269053723413336 18.606251915963504 31.899074564217404 17.225619796405752 8 27.603069374798245 27.674258249331 27.862740043924877 16.859932331945878 9 30.595814906347556 25.72731041883455 25.409038436524455 18.267836238293444 10-11 24.79712547515536 28.509179597246266 28.83218160780423 17.86151331979414 12-13 25.66480718081593 25.457042868716613 28.926739401113576 19.95141054935388 14-15 24.277753544919385 26.054410513474167 30.083612169294316 19.58422377231213 16-17 23.138117383221335 27.498439577239758 31.10227938589565 18.26116365364326 18-19 22.18643100960957 27.844834085680382 31.219025760528268 18.749709144181775 20-21 22.17983936968892 28.34674425783931 30.635845081314038 18.837571291157733 22-23 22.757260040048383 27.76532053953507 30.596883544586863 18.880535875829683 24-25 22.609781091033796 27.903054600680978 30.60896048860546 18.87820381967976 26-27 21.995603841197156 28.50873383099505 30.404208594785437 19.091453733022355 28-29 21.456215942264194 28.574127652166414 30.210058210077733 19.759598195491655 30-31 21.57775932907149 28.65688487125106 29.9489751671275 19.81638063254995 32-33 20.8813504235143 28.474652067434832 31.40438864834241 19.239608860708454 34-35 20.618857992061734 29.18812311092492 31.188635927155655 19.004382969857687 36-37 20.91294210986006 29.981210146398762 30.726287813638535 18.37955993010264 38-39 21.895254125795656 29.103643082399827 29.874293953584992 19.126808838219524 40-41 21.463649192452063 28.51141247112336 29.544199713611363 20.48073862281321 42-43 21.84312020132171 28.688608234351175 30.338391157650747 19.12988040667637 44-45 21.068693833355837 29.20668551200194 30.858676480740556 18.865944173901674 46-47 21.320921778156723 29.0459273846214 30.671262372158857 18.96188846506302 48-49 21.285580062638793 29.735503144058427 29.75988326869647 19.219033524606306 50-51 20.797313771200034 29.523096618077048 29.78226840493453 19.897321205788387 52-53 21.252299302836715 28.95549331756203 29.864787144432874 19.927420235168377 54-55 21.922225856100848 29.14219059344949 29.307731187652454 19.62785236279721 56-57 22.206253043438767 28.979993087791765 28.794357324506997 20.01939654426247 58-59 21.812126937592797 28.636143608675134 29.79613846870985 19.755590985022213 60-61 20.70026475946468 29.045583256712437 29.93846439510484 20.31568758871804 62-63 21.30521309223445 29.147671776439726 29.514466380825542 20.032648750500275 64-65 21.081063803233576 29.10146702626526 29.54497286417756 20.272496306323603 66-67 21.19371529453448 28.766435144177223 29.356990574163554 20.682858987124746 68-69 21.267421218822093 28.715954907666752 29.927492222002112 20.089131651509046 70-71 20.710225393254998 30.12778560923354 29.60239388094717 19.559595116564292 72-73 20.325282190982225 31.133797801176904 28.941447699329025 19.599472308511846 74-75 21.070670323007285 30.46989187498181 28.628858020451002 19.830579781559905 76-77 21.395628209979282 29.658585316219277 29.215169797510644 19.730616676290797 78-79 21.389629109121344 29.519720505144242 29.80101889497101 19.2896314907634 80-81 20.393210677659873 30.366674300837214 29.44467007659618 19.795444944906738 82-83 19.58468344844564 30.144343281542806 29.746135258371968 20.52483801163958 84-85 19.727394465835637 29.467023685008343 30.2229152104514 20.58266663870462 86-87 19.66216386809564 29.703627296634956 30.997414949475864 19.636793885793544 88-89 19.971036843928214 30.244629120311327 30.409175847122427 19.37515818863803 90-91 20.360507838388404 29.653612357360064 29.833112927970713 20.15276687628082 92-93 19.73201213603696 30.553474616433157 29.719203503360536 19.995309744169347 94-95 19.2828537980364 31.076484697503247 29.809899197943857 19.8307623065165 96-97 19.887639122629068 31.56090978350762 29.190719905270583 19.360731188592727 98-99 20.433605980412658 30.487232025033222 28.790774129429415 20.288387865124704 100-101 20.285726444335896 30.272245209030412 28.36903115303554 21.07299719359815 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 760.0 1 584.5 2 319.0 3 247.5 4 269.0 5 299.0 6 419.5 7 580.0 8 743.0 9 880.5 10 982.0 11 1040.5 12 971.0 13 832.5 14 750.0 15 661.0 16 597.0 17 615.0 18 642.5 19 719.5 20 866.5 21 1076.5 22 1390.5 23 1875.5 24 2563.0 25 3426.0 26 4731.5 27 6535.5 28 8528.0 29 10629.5 30 12953.0 31 15679.5 32 21260.0 33 31831.5 34 48092.0 35 71284.0 36 103897.0 37 144312.5 38 185463.5 39 205584.0 40 201583.5 41 179187.0 42 152166.5 43 143125.0 44 135821.5 45 126540.0 46 125447.0 47 112396.5 48 84015.0 49 63202.0 50 50813.5 51 42023.0 52 35207.0 53 28094.0 54 21257.0 55 15704.0 56 11610.5 57 8370.5 58 6494.0 59 5132.0 60 4005.0 61 3154.5 62 2496.0 63 2010.5 64 1618.0 65 1301.5 66 1063.0 67 888.5 68 755.5 69 625.5 70 511.5 71 442.5 72 377.0 73 301.0 74 240.0 75 192.0 76 151.0 77 122.5 78 99.0 79 69.0 80 50.5 81 43.5 82 38.5 83 31.5 84 19.5 85 16.5 86 14.0 87 10.5 88 8.5 89 5.0 90 3.5 91 2.0 92 3.0 93 4.5 94 3.5 95 1.0 96 0.5 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30027799157581603 2 0.2759212530405329 3 0.24624662659171287 4 0.23374350081026748 5 0.24019803652211755 6 0.18636964435914172 7 0.1536910201576368 8 0.15141772456101038 9 0.1308768750629216 10-11 0.11662818301978092 12-13 0.13741259990322255 14-15 0.1402542193990056 16-17 0.13451008856110133 18-19 0.12066734216021537 20-21 0.12740603982164372 22-23 0.15665442334609625 24-25 0.5668624948444903 26-27 0.2255636961188349 28-29 0.19789850059917577 30-31 0.2973551829515821 32-33 0.32861299740519545 34-35 0.33748290968846106 36-37 0.32757783601744594 38-39 0.33496604670648183 40-41 0.33809182815184313 42-43 0.34937711700652435 44-45 0.3302164826921016 46-47 0.3502701974194848 48-49 0.3456830116620064 50-51 0.3507979267544159 52-53 0.3366101265576134 54-55 0.32861299740519545 56-57 0.33723934230310826 58-59 0.32940459140759215 60-61 0.3073008511868227 62-63 0.2939452395566424 64-65 0.304357745280476 66-67 0.320656462817003 68-69 0.3076459049827392 70-71 0.28909418913169854 72-73 0.2851565164018277 74-75 0.2772405763778607 76-77 0.2794732774102616 78-79 0.28803873046183626 80-81 0.2972131019767929 82-83 0.3200272470715081 84-85 0.3115429831483845 86-87 0.3136336032059963 88-89 0.31868762645206755 90-91 0.3118271450979628 92-93 0.29341751022171125 94-95 0.28962191846662966 96-97 0.3063671762096368 98-99 0.3153182776213534 100-101 0.3145469809010694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2463384.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.22909693478149 #Duplication Level Percentage of deduplicated Percentage of total 1 89.19620114190838 39.45067426519733 2 4.436795301267442 3.9247089891908145 3 2.0124656053420753 2.67028609009765 4 0.8034148097627444 1.4213724599934179 5 0.6155805162775951 1.3613285162802296 6 0.3835147261889635 1.0177506000316707 7 0.3429528208338208 1.0617945489701064 8 0.26494760111438403 0.9374714505860727 9 0.1935480012721908 0.7704407978820844 >10 1.3624814587445306 12.285963093029602 >50 0.18489687745338027 5.719238104587528 >100 0.17153199117757892 16.639564578318097 >500 0.02433609695917915 7.362852366919503 >1k 0.00724093236789147 5.135433822922481 >5k 9.211933013047998E-5 0.24112031599332348 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5918 0.24023863108634302 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4848 0.19680244736508803 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 4503 0.1827973227073002 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3773 0.15316329082270566 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 3634 0.14752064639536508 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3601 0.14618102577592448 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3035 0.12320450242430736 No Hit TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC 3016 0.12243320570402341 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2861 0.11614104824907526 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2825 0.11467964393695826 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 2661 0.10802213540398087 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.742695414113268E-4 0.0 0.0 2 0.0010554586698622707 0.0 0.0 3 0.0011772423625386866 0.0 0.0 4 0.0012584314909896304 0.0 0.0 5 0.0013396206194405745 0.0 0.0 6 0.0013396206194405745 0.0 0.0 7 0.001623782569018878 0.0 0.0 8 0.0018673499543717098 0.0 0.0 9 0.0021921064681754854 0.0 0.0 10-11 0.003552024369728796 0.0 0.0 12-13 0.004810455860718427 0.0 0.0 14-15 0.006170373762271737 0.0 2.0297282112735976E-5 16-17 0.00791594002396703 0.0 4.059456422547195E-5 18-19 0.009560019875098646 0.0 4.059456422547195E-5 20-21 0.01175212634327413 0.0 4.059456422547195E-5 22-23 0.01412690835046424 0.0 8.11891284509439E-5 24-25 0.016664068614556236 0.0 8.11891284509439E-5 26-27 0.01958687723879022 0.0 8.11891284509439E-5 28-29 0.022956226069504388 0.0 8.11891284509439E-5 30-31 0.03600737846799362 0.0 8.11891284509439E-5 32-33 0.040188618583217234 0.0 8.11891284509439E-5 34-35 0.0437812375171715 0.0 8.11891284509439E-5 36-37 0.047800099375493225 0.0 1.2178369267641586E-4 38-39 0.05216401502973146 0.0 1.2178369267641586E-4 40-41 0.05685268719777347 0.0 1.2178369267641586E-4 42-43 0.061216602852011706 0.0 1.2178369267641586E-4 44-45 0.06675776086878862 0.0 1.2178369267641586E-4 46-47 0.07242070257824196 0.0 1.2178369267641586E-4 48-49 0.07934207577868493 0.0 1.2178369267641586E-4 50-51 0.08666939462138262 0.0 1.2178369267641586E-4 52-53 0.09393582161774211 0.0 1.2178369267641586E-4 54-55 0.1020547344628365 0.0 1.2178369267641586E-4 56-57 0.11126970054201862 0.0 1.2178369267641586E-4 58-59 0.12105299052035737 0.0 1.2178369267641586E-4 60-61 0.13158727993686734 0.0 1.2178369267641586E-4 62-63 0.1432379198695778 0.0 1.2178369267641586E-4 64-65 0.15533510000876843 0.0 1.2178369267641586E-4 66-67 0.16777733394387556 0.0 1.2178369267641586E-4 68-69 0.18180275588377615 0.0 1.2178369267641586E-4 70-71 0.1980202842918522 0.0 1.2178369267641586E-4 72-73 0.21437989367471738 0.0 1.2178369267641586E-4 74-75 0.23331725788590005 0.0 1.2178369267641586E-4 76-77 0.2539798910766653 0.0 1.2178369267641586E-4 78-79 0.27547471283405267 0.0 1.2178369267641586E-4 80-81 0.2986542090067972 0.0 1.2178369267641586E-4 82-83 0.3265223773475836 0.0 1.2178369267641586E-4 84-85 0.3582064347255645 0.0 1.2178369267641586E-4 86-87 0.39196081487904444 0.0 1.4208097478915184E-4 88-89 0.429815246019297 0.0 1.623782569018878E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 600 0.0 67.94987 9 AGAGCGG 575 0.0 67.609085 8 CGGGAGA 740 0.0 58.36051 4 GGATCGG 470 0.0 55.561954 1 GAGAGCG 685 0.0 54.6803 7 ATTCGGA 150 0.0 53.797775 2 CGGAAAG 340 0.0 53.040215 5 AAGCGGC 180 0.0 52.67432 9 TTGTGTT 31515 0.0 51.841797 1 TCGGAGA 250 0.0 51.254642 4 CGCCGAG 40 5.40697E-4 47.46862 2 TTCGGAG 150 0.0 47.459934 3 GATCGAC 50 2.8588676E-5 47.412666 7 ATCGACT 40 5.440684E-4 47.408813 8 TCGGGAG 860 0.0 46.356213 3 TCGGAAA 245 0.0 44.55241 4 CGGAGAG 490 0.0 43.58299 5 GATACGT 55 5.0227936E-5 43.097168 9 GTTCGGA 135 0.0 42.19433 2 ACCGGAA 130 5.456968E-12 40.1584 3 >>END_MODULE