FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_35008-1.342000000357b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_35008-1.342000000357b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463384
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC59180.24023863108634302No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG48480.19680244736508803No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC45030.1827973227073002No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA37730.15316329082270566No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG36340.14752064639536508No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA36010.14618102577592448No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA30350.12320450242430736No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC30160.12243320570402341No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA28610.11614104824907526No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT28250.11467964393695826No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG26610.10802213540398087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC6000.067.949879
AGAGCGG5750.067.6090858
CGGGAGA7400.058.360514
GGATCGG4700.055.5619541
GAGAGCG6850.054.68037
ATTCGGA1500.053.7977752
CGGAAAG3400.053.0402155
AAGCGGC1800.052.674329
TTGTGTT315150.051.8417971
TCGGAGA2500.051.2546424
CGCCGAG405.40697E-447.468622
TTCGGAG1500.047.4599343
GATCGAC502.8588676E-547.4126667
ATCGACT405.440684E-447.4088138
TCGGGAG8600.046.3562133
TCGGAAA2450.044.552414
CGGAGAG4900.043.582995
GATACGT555.0227936E-543.0971689
GTTCGGA1350.042.194332
ACCGGAA1305.456968E-1240.15843
Produced by FastQC (version 0.11.2)