##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_35005-1.3420000003578b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2673689 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04595859877495 33.0 33.0 33.0 33.0 33.0 2 32.001191612038646 33.0 33.0 33.0 33.0 33.0 3 31.972957587812196 33.0 33.0 33.0 33.0 33.0 4 35.72786924732084 37.0 37.0 37.0 33.0 37.0 5 35.750537927185995 37.0 37.0 37.0 33.0 37.0 6 35.7842340676122 37.0 37.0 37.0 33.0 37.0 7 35.72976026755543 37.0 37.0 37.0 33.0 37.0 8 35.662145821746655 37.0 37.0 37.0 33.0 37.0 9 35.58690146834579 37.0 37.0 37.0 33.0 37.0 10-11 35.546354680742596 37.0 37.0 37.0 33.0 37.0 12-13 35.52847432891409 37.0 37.0 37.0 33.0 37.0 14-15 37.27740679637759 40.0 37.0 40.0 33.0 40.0 16-17 37.273890680628895 40.0 37.0 40.0 33.0 40.0 18-19 37.25814483285079 40.0 37.0 40.0 33.0 40.0 20-21 37.25106809355913 40.0 37.0 40.0 33.0 40.0 22-23 37.25338829609577 40.0 37.0 40.0 33.0 40.0 24-25 37.00576469439789 40.0 37.0 40.0 33.0 40.0 26-27 36.939644625833445 40.0 37.0 40.0 33.0 40.0 28-29 36.92497033125393 40.0 37.0 40.0 33.0 40.0 30-31 36.91747245098439 40.0 37.0 40.0 33.0 40.0 32-33 36.7992715308325 40.0 37.0 40.0 33.0 40.0 34-35 36.751979007281705 40.0 37.0 40.0 33.0 40.0 36-37 36.66970691056439 40.0 37.0 40.0 33.0 40.0 38-39 36.598688553530344 40.0 37.0 40.0 33.0 40.0 40-41 36.505625186773784 40.0 37.0 40.0 33.0 40.0 42-43 36.39655752782018 40.0 37.0 40.0 33.0 40.0 44-45 36.29618871155172 38.5 37.0 40.0 33.0 40.0 46-47 36.18940422764203 37.0 37.0 40.0 33.0 40.0 48-49 36.06489086801045 37.0 37.0 40.0 30.0 40.0 50-51 34.897601403902996 37.0 35.0 38.5 27.0 38.5 52-53 35.32630216154534 37.0 37.0 38.5 30.0 40.0 54-55 36.088702350946576 37.0 37.0 40.0 33.0 40.0 56-57 36.15544066643503 37.0 37.0 40.0 33.0 40.0 58-59 35.955219922735964 37.0 37.0 40.0 30.0 40.0 60-61 35.6947561964013 37.0 37.0 40.0 27.0 40.0 62-63 35.43050930755223 37.0 37.0 40.0 27.0 40.0 64-65 35.18124303163158 37.0 37.0 40.0 27.0 40.0 66-67 34.89058544206151 37.0 33.0 37.0 27.0 40.0 68-69 34.67230799842465 37.0 33.0 37.0 27.0 40.0 70-71 34.37027081309756 37.0 33.0 37.0 27.0 40.0 72-73 34.150418204959514 37.0 33.0 37.0 27.0 38.5 74-75 33.9177413678255 37.0 33.0 37.0 27.0 37.0 76-77 33.68451959072278 37.0 33.0 37.0 27.0 37.0 78-79 33.449555090363916 37.0 33.0 37.0 27.0 37.0 80-81 33.21038479045244 37.0 33.0 37.0 27.0 37.0 82-83 33.02044721730912 37.0 33.0 37.0 27.0 37.0 84-85 32.78146504698191 37.0 33.0 37.0 27.0 37.0 86-87 32.65573595133914 37.0 33.0 37.0 27.0 37.0 88-89 32.553629274010554 35.0 33.0 37.0 24.5 37.0 90-91 32.42985665124104 33.0 33.0 37.0 22.0 37.0 92-93 32.280720008946446 33.0 33.0 37.0 22.0 37.0 94-95 32.14457534141032 33.0 33.0 37.0 22.0 37.0 96-97 31.991362121772575 33.0 33.0 37.0 22.0 37.0 98-99 31.844060958473477 33.0 33.0 37.0 22.0 37.0 100-101 30.533548591477917 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.2018928579989634 2306 2 0.26798437462841207 2306 3 0.23766813082858818 2306 4 0.32495713029610584 2306 5 0.2542775761516154 2306 6 0.18911739959417417 2306 7 0.23619066915977527 2306 8 0.35259674390547957 2306 9 0.34026847669399984 2306 10-11 0.3300128122172694 2306 12-13 0.37285583071713546 2306 14-15 0.5347259794725119 2306 16-17 0.5551392794963732 2306 18-19 0.4251072765136428 2306 20-21 0.49024684362412785 2306 22-23 0.4747934068469206 2306 24-25 0.43988190387167947 2306 26-27 0.45841446588828205 2306 28-29 0.3602832383624346 2306 30-31 0.29992945403881066 2306 32-33 0.27487671247860135 2306 34-35 0.24878770614060386 2306 36-37 0.3125691945817408 2306 38-39 0.3217369163750803 2306 40-41 0.3592594355074965 2306 42-43 0.30146662172967353 2306 44-45 0.47809245457900573 2306 46-47 0.46394382800494327 2306 48-49 0.40128920286655045 2306 50-51 0.4651127625757354 2306 52-53 0.3383247455809979 2306 54-55 0.4712339063881572 2306 56-57 0.46159496289530466 2306 58-59 0.543862248716799 2306 60-61 0.36190479651354934 2306 62-63 0.4326634728818206 2306 64-65 0.4849556003174271 2306 66-67 0.5109195305336272 2306 68-69 0.388262559441813 2306 70-71 0.35684491887306535 2306 72-73 0.4394039841124453 2306 74-75 0.4263053779921222 2306 76-77 0.37090454472053835 2306 78-79 0.4510205872193396 2306 80-81 0.3921568719989139 2306 82-83 0.4131675629264606 2306 84-85 0.5363660813051467 2306 86-87 0.6628133360489414 2306 88-89 0.4936600281033563 2306 90-91 0.5057062428907741 2306 92-93 0.37579367171169054 2306 94-95 0.22196368934307031 2306 96-97 0.19532960506103336 2306 98-99 0.45244293612372033 2306 100-101 0.2624363675887338 2307 1 0.25378061750897984 2307 2 0.24330188109062334 2307 3 0.1970242681193426 2307 4 0.38245154372349077 2307 5 0.2823306935408567 2307 6 0.24135938400080903 2307 7 0.1813696379613674 2307 8 0.21688354231485363 2307 9 0.2834474875262316 2307 10-11 0.24941450605206938 2307 12-13 0.26816544475911286 2307 14-15 0.3944054024385224 2307 16-17 0.4782529793835977 2307 18-19 0.35615157447351464 2307 20-21 0.30992523917448267 2307 22-23 0.46618074488301176 2307 24-25 0.3375281880128256 2307 26-27 0.3103812663524863 2307 28-29 0.30647724712815716 2307 30-31 0.2585897507284258 2307 32-33 0.2918779897430568 2307 34-35 0.21775753514594953 2307 36-37 0.36807987853700297 2307 38-39 0.256158843293143 2307 40-41 0.24540380141690576 2307 42-43 0.19940653659426744 2307 44-45 0.2203834513823466 2307 46-47 0.3489023460979581 2307 48-49 0.28788983472718144 2307 50-51 0.08192268367488253 2307 52-53 0.17483477806133862 2307 54-55 0.15504930781579418 2307 56-57 0.05403271668448184 2307 58-59 0.3440179955896028 2307 60-61 0.27324022670882897 2307 62-63 0.16632966553626716 2307 64-65 0.1662880416671655 2307 66-67 0.12172163860373786 2307 68-69 0.08012916352952004 2307 70-71 0.1591430608852562 2307 72-73 0.19534104556358045 2307 74-75 0.3143156708289325 2307 76-77 0.17103051988976858 2307 78-79 0.09430992848245268 2307 80-81 0.25627006051333723 2307 82-83 0.26400502343516763 2307 84-85 0.266799767462345 2307 86-87 0.22803274766668125 2307 88-89 0.07260956564956444 2307 90-91 0.11333118480676063 2307 92-93 0.1613548624864336 2307 94-95 0.30921567937713945 2307 96-97 0.10010988988867098 2307 98-99 0.21827856362950726 2307 100-101 0.2070041408287615 2201 1 0.3324632033551964 2201 2 0.294363714325236 2201 3 0.24230563784317383 2201 4 0.26904402736840183 2201 5 0.25138301874822133 2201 6 0.2505129122420442 2201 7 0.22552850143488 2201 8 0.19217331974360263 2201 9 0.25555076697482804 2201 10-11 0.2400703070357082 2201 12-13 0.26762648647152787 2201 14-15 0.48925751334923717 2201 16-17 0.3953893905137136 2201 18-19 0.505089297560545 2201 20-21 0.46619774198628505 2201 22-23 0.5077691577789665 2201 24-25 0.5740541199037565 2201 26-27 0.4793237811658031 2201 28-29 0.40781937416658565 2201 30-31 0.317835896827269 2201 32-33 0.4066446038338398 2201 34-35 0.32468257790544897 2201 36-37 0.4016577895661868 2201 38-39 0.5581761378756056 2201 40-41 0.47771290325662363 2201 42-43 0.42373408658018263 2201 44-45 0.42653605011742 2201 46-47 0.46155594200014605 2201 48-49 0.5030353501372389 2201 50-51 0.3127636538219818 2201 52-53 0.302610041661751 2201 54-55 0.4875129206443063 2201 56-57 0.4799342156518236 2201 58-59 0.4218287534587972 2201 60-61 0.4903851786473652 2201 62-63 0.5045346981798957 2201 64-65 0.3714621077796849 2201 66-67 0.5148722430045893 2201 68-69 0.4813218818802838 2201 70-71 0.430231204246617 2201 72-73 0.493757890256191 2201 74-75 0.5031780129626284 2201 76-77 0.49658517329932295 2201 78-79 0.5484422378061353 2201 80-81 0.5098422259077608 2201 82-83 0.4311926794158438 2201 84-85 0.5637833092135907 2201 86-87 0.43691895969612915 2201 88-89 0.44853091922350785 2201 90-91 0.43314774598889727 2201 92-93 0.4066591925653853 2201 94-95 0.4306474516281682 2201 96-97 0.4390842608082224 2201 98-99 0.6161110839222452 2201 100-101 0.2622688282462633 1101 1 0.14478108161905823 1101 2 -0.013923860543279432 1101 3 0.06828874733076873 1101 4 0.13212607441775504 1101 5 0.16922028379949694 1101 6 0.15749587758995176 1101 7 0.2444149538415772 1101 8 0.3104102297211071 1101 9 0.3070165263230322 1101 10-11 0.2970926096401527 1101 12-13 0.23998728679154624 1101 14-15 0.33308289331515084 1101 16-17 0.2295055920218232 1101 18-19 0.35443985897838104 1101 20-21 0.3663798689147839 1101 22-23 0.23321816120051864 1101 24-25 0.14968248792268923 1101 26-27 0.1481487353588733 1101 28-29 0.17342561030559267 1101 30-31 0.07540582638160487 1101 32-33 0.11092760860208273 1101 34-35 0.1652783151093189 1101 36-37 0.12485798546322968 1101 38-39 0.15864554723936664 1101 40-41 0.10715843901801492 1101 42-43 0.049781539690130217 1101 44-45 0.10534413124438657 1101 46-47 0.03466622713502687 1101 48-49 3.271233336477053E-4 1101 50-51 0.020574653881389793 1101 52-53 0.06931466788095975 1101 54-55 0.24823948849567756 1101 56-57 0.23981910329140277 1101 58-59 0.34590577140397727 1101 60-61 0.22459793712783238 1101 62-63 0.09208615957767563 1101 64-65 0.15633498031741766 1101 66-67 0.11634668396484926 1101 68-69 0.11030644065708373 1101 70-71 0.0972587739726265 1101 72-73 -0.03123092541822814 1101 74-75 0.05025706589429291 1101 76-77 0.06171439847167903 1101 78-79 0.18735301534210436 1101 80-81 0.13704653497813268 1101 82-83 0.04700631885146578 1101 84-85 0.10464950563319775 1101 86-87 0.11095661961536507 1101 88-89 0.02166664688174791 1101 90-91 -0.025721039960352243 1101 92-93 0.1528168523018536 1101 94-95 0.2041359892002319 1101 96-97 -0.04613841007934383 1101 98-99 0.13447923578150167 1101 100-101 0.24046001645156778 2304 1 0.011225175457788339 2304 2 -0.07309584233616206 2304 3 -0.046615847841405156 2304 4 0.08434216760763746 2304 5 0.06359812672422294 2304 6 0.08482727692859271 2304 7 0.1336611668444334 2304 8 -0.008061680617721834 2304 9 0.08195233492151743 2304 10-11 0.06450006384666551 2304 12-13 0.0705670452741245 2304 14-15 0.07480157751400185 2304 16-17 0.1423559654453257 2304 18-19 0.22293115900615135 2304 20-21 0.06907857252851102 2304 22-23 0.005223520157834116 2304 24-25 0.06009892727811916 2304 26-27 -0.1031048114231794 2304 28-29 -0.027353504412175766 2304 30-31 -0.018694921318136437 2304 32-33 -0.060850306687555644 2304 34-35 -0.18945347636422127 2304 36-37 0.028498595785912073 2304 38-39 -0.11329537322143324 2304 40-41 -0.05759364525243171 2304 42-43 0.026052668976234372 2304 44-45 -0.199542981230735 2304 46-47 -0.1629314669551718 2304 48-49 -0.12771953362621957 2304 50-51 -0.06774668743160106 2304 52-53 -0.10431578150826937 2304 54-55 0.028407315410582612 2304 56-57 0.10631358512740974 2304 58-59 0.07851362739663159 2304 60-61 -0.021588321328870563 2304 62-63 -0.005465293835037244 2304 64-65 -0.12228748548124457 2304 66-67 0.13161773553716216 2304 68-69 0.047694412150647736 2304 70-71 -0.03887399374897882 2304 72-73 -0.012837271515167004 2304 74-75 0.1825444542243133 2304 76-77 -0.02506662052478248 2304 78-79 0.054998572215716024 2304 80-81 0.11499490627333131 2304 82-83 0.05751405738194393 2304 84-85 0.0894723501558019 2304 86-87 0.11772430904856179 2304 88-89 -0.0505869311021101 2304 90-91 0.06833833144163037 2304 92-93 0.05520958449216806 2304 94-95 2.443497511563919E-4 2304 96-97 -0.12951630126026004 2304 98-99 -0.05481345045883401 2304 100-101 -0.010650893538223016 2202 1 0.13141383849708177 2202 2 0.05625744988651604 2202 3 0.14695564563784558 2202 4 0.1520726824120473 2202 5 0.1738656732208952 2202 6 0.17844249872040763 2202 7 0.20461591115294908 2202 8 0.1694044921804263 2202 9 0.1698341507543617 2202 10-11 0.22762694228355684 2202 12-13 0.175988111224207 2202 14-15 0.2649715453214938 2202 16-17 0.3412570137333688 2202 18-19 0.38286483921401526 2202 20-21 0.28507500811087283 2202 22-23 0.30863034515335386 2202 24-25 0.25182952815208637 2202 26-27 0.3235784218542932 2202 28-29 0.215631160091732 2202 30-31 0.23313925821761217 2202 32-33 0.07493490638526623 2202 34-35 0.21770832852854483 2202 36-37 0.1907466400597997 2202 38-39 0.19679313437696067 2202 40-41 0.15300325955126937 2202 42-43 0.24839913485187992 2202 44-45 0.24973964504299317 2202 46-47 0.24781565055945975 2202 48-49 0.3088844605345713 2202 50-51 0.04532807802442562 2202 52-53 0.18269034774650805 2202 54-55 0.32103063894371786 2202 56-57 0.20721617733252629 2202 58-59 0.22179498289963107 2202 60-61 0.2350007564803107 2202 62-63 0.2389737505154983 2202 64-65 0.38760517831631347 2202 66-67 0.4092854071192136 2202 68-69 0.3525835362489005 2202 70-71 0.3670297552380646 2202 72-73 0.2949542104087186 2202 74-75 0.3130505271960047 2202 76-77 0.3750444163128037 2202 78-79 0.23806468981553763 2202 80-81 0.30026255502205856 2202 82-83 0.41892998334363796 2202 84-85 0.42370781333714547 2202 86-87 0.3576770668219851 2202 88-89 0.3005097177157978 2202 90-91 0.18939186901588556 2202 92-93 0.3846704992230201 2202 94-95 0.30051292588154155 2202 96-97 0.32980667634085137 2202 98-99 0.31992431748377825 2202 100-101 0.4191223078526214 1102 1 0.1376017665430922 1102 2 0.06690976871455234 1102 3 0.008024189019309347 1102 4 0.046382506756771136 1102 5 0.13600238209507864 1102 6 0.03784965759046344 1102 7 0.10698454426641035 1102 8 0.05680359340190222 1102 9 0.14813703918337495 1102 10-11 0.11789584326709956 1102 12-13 0.1574401553205078 1102 14-15 0.15913816972429373 1102 16-17 0.1373930848374414 1102 18-19 0.1446103006542785 1102 20-21 0.07191352465229528 1102 22-23 0.10619899576902725 1102 24-25 0.1431487277837391 1102 26-27 0.2576195175857734 1102 28-29 0.1225891524550633 1102 30-31 0.15767479484116365 1102 32-33 0.14777644073286922 1102 34-35 0.13649440405789903 1102 36-37 0.17135365533214042 1102 38-39 0.1271377158668443 1102 40-41 0.12751590738780294 1102 42-43 0.05664322511955788 1102 44-45 0.20079567831842127 1102 46-47 0.1971898303503039 1102 48-49 0.27413742555234677 1102 50-51 0.27303106808562205 1102 52-53 0.32550867177018716 1102 54-55 0.3595206784264633 1102 56-57 0.4332755171343905 1102 58-59 0.41641025073158744 1102 60-61 0.4118236801391575 1102 62-63 0.5139355390032208 1102 64-65 0.3677022924065625 1102 66-67 0.4562868102797566 1102 68-69 0.3791221815045205 1102 70-71 0.3126552953454791 1102 72-73 0.35736560130113304 1102 74-75 0.3204302964253216 1102 76-77 0.31957151010644225 1102 78-79 0.20846323978353354 1102 80-81 0.33070994545172994 1102 82-83 0.3960240421703034 1102 84-85 0.515893107432376 1102 86-87 0.4286884097821968 1102 88-89 0.28900333719708726 1102 90-91 0.1935638932498236 1102 92-93 0.22552216098873856 1102 94-95 0.43000760577167085 1102 96-97 0.3403377558662015 1102 98-99 0.36089110272132885 1102 100-101 0.47122603764758964 2305 1 0.14878345916628888 2305 2 0.16272634152227994 2305 3 0.19716011320050342 2305 4 0.293542324278242 2305 5 0.17399285514105856 2305 6 0.18167349109663178 2305 7 0.26614528565130513 2305 8 0.2966273773046666 2305 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1213 76-77 -0.036138525235884345 1213 78-79 -0.050543904213661506 1213 80-81 -0.0167786839536177 1213 82-83 0.002147532683586917 1213 84-85 0.06831483447850673 1213 86-87 -0.03252758959322932 1213 88-89 -0.11163125874518443 1213 90-91 -0.07428368453806655 1213 92-93 -0.11140615350697658 1213 94-95 -0.027212026003773815 1213 96-97 -0.0015419152821749549 1213 98-99 -0.138339501091842 1213 100-101 0.12981173454706507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1239.0 3 240.0 4 252.0 5 291.0 6 800.0 7 2332.0 8 6678.0 9 11597.0 10 12926.0 11 9537.0 12 6913.0 13 5408.0 14 5062.0 15 5021.0 16 5027.0 17 5383.0 18 5913.0 19 6678.0 20 7640.0 21 8628.0 22 9798.0 23 11107.0 24 12658.0 25 14882.0 26 17259.0 27 20984.0 28 25860.0 29 32077.0 30 40191.0 31 52145.0 32 71638.0 33 102558.0 34 156879.0 35 264501.0 36 518783.0 37 930428.0 38 283464.0 39 912.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.59291758210636 25.08169091855066 23.98875251749823 14.336638981844754 2 32.94767772191918 22.040236943807415 32.52434411981451 12.487741214458897 3 39.058020841222344 18.594233533567238 25.96072156189942 16.387024063311006 4 31.69392392858455 17.801005118562156 33.85924741316551 16.645823539687786 5 30.892446024690173 16.525897913943176 35.65214066103142 16.929515400335234 6 25.204208920179877 17.163966301359494 40.700704796656666 16.931119981803963 7 33.877773628258055 17.09933837843345 30.22917210588792 18.793715887420575 8 30.754541708806233 26.397572760984378 24.93980597070832 17.90807955950107 9 29.71504540773336 26.799213556783073 24.8399588053553 18.645782230128265 10-11 24.530323871381558 28.112606568416936 30.215564151486333 17.141505408715165 12-13 25.24837168471037 25.813558414301234 29.826586434694548 19.111483466293848 14-15 23.51548368841501 26.779542246788836 30.862433776232724 18.842540288563438 16-17 22.619899624406077 27.91864266370955 31.589063022555486 17.872394689328885 18-19 22.133159034769907 28.043351962085193 31.513003023575802 18.310485979569098 20-21 22.160609477473578 28.2703447821318 31.145545176985962 18.423500563408655 22-23 22.27645589952943 27.742910892528165 31.135321057958777 18.84531214998363 24-25 22.280586536913578 27.7581182191449 31.038524961370474 18.92277028257105 26-27 21.770509980736136 28.290610337261953 31.04220544448772 18.896674237514194 28-29 21.332896858203547 28.59121343334035 30.480286289709056 19.595603418747043 30-31 21.26264485867188 28.892973349737034 30.31975026058289 19.52463153100819 32-33 20.798882022236846 29.076090372621106 31.36850392607507 18.75652367906698 34-35 21.43392198777456 28.80267941851109 31.22603775114388 18.537360842570465 36-37 21.030667244811283 29.095803454345308 31.508794221355714 18.364735079487694 38-39 21.30654471393686 28.98165567643497 30.809070196750852 18.902729412877317 40-41 21.27312665552289 28.980348019152085 30.072289446011098 19.67423587931393 42-43 21.325879133871567 28.81967668442012 30.67059644855814 19.18384773315017 44-45 20.829326623137455 28.79662098532329 31.32669781471446 19.047354576824794 46-47 21.101915522149582 28.647799267376445 30.95372993744418 19.296555273029796 48-49 20.942807182027444 29.740937173586428 29.874233074723396 19.442022569662733 50-51 20.99631318330207 29.207658144167763 29.748622722013646 20.04740595051652 52-53 21.257965204634854 28.847311819079263 29.81243407871956 20.08228889756633 54-55 21.96314955015808 28.912330077960092 29.455986790877446 19.66853358100438 56-57 21.979443130096435 29.195607759190096 29.13132062952599 19.693628481187478 58-59 21.61319950202595 29.21518426822301 29.76969169327125 19.40192453647979 60-61 20.876338602610208 29.517698808761622 29.694443970264345 19.91151861836383 62-63 21.117638464087857 29.252167415256373 29.42342993569825 20.20676418495752 64-65 20.829752056193943 29.145299369757748 29.633015854912596 20.391932719135706 66-67 21.20802896153621 28.34455012510075 29.81491543129365 20.632505482069394 68-69 21.27310582377717 28.708032279261875 30.11079089969279 19.90807099726817 70-71 20.5406160678785 30.205987381561478 29.505934117046895 19.74746243351313 72-73 20.363079803739836 30.45759030296847 29.06063757922292 20.118692314068774 74-75 20.88354159247593 29.752093702455813 29.085506922687298 20.278857782380964 76-77 20.812596744182034 29.369086344850555 29.345269507209 20.47304740375841 78-79 20.67864007911672 29.55700899628254 29.50260030769698 20.261750616903754 80-81 19.969347328048116 30.012833532094895 29.494690459032295 20.523128680824694 82-83 19.437323551527832 29.723397086170642 29.687675885638125 21.1516034766634 84-85 19.602727324230496 29.342297350077267 30.01339800091771 21.041577324774522 86-87 19.9164467683483 29.33713085503895 30.69843057697842 20.047991799634328 88-89 20.141713469233377 30.007399358221992 29.914625964693204 19.936261207851423 90-91 20.347791568935957 29.64424489860238 29.50705452665382 20.500909005807845 92-93 19.834089928225552 30.219918624238336 29.66874817009571 20.277243277440405 94-95 19.450079964259437 30.639112022547167 29.78847310558227 20.12233490761113 96-97 19.82500332029975 31.164145865082528 29.278778369804105 19.732072444813618 98-99 20.464090217856644 30.267976840701593 28.78245590469433 20.485477036747433 100-101 20.45148280215153 30.109521950447128 28.141732611404098 21.297262635997242 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 870.0 1 656.0 2 355.5 3 297.5 4 346.0 5 373.0 6 483.0 7 660.5 8 799.5 9 975.5 10 1104.0 11 1136.5 12 1094.5 13 956.0 14 821.5 15 703.0 16 628.5 17 610.5 18 639.0 19 687.0 20 807.5 21 986.5 22 1234.5 23 1657.0 24 2239.5 25 3223.0 26 5157.5 27 8049.0 28 11213.5 29 14339.5 30 17801.5 31 21173.5 32 26676.5 33 37907.0 34 55283.0 35 79681.0 36 114753.0 37 153212.0 38 186945.5 39 206669.5 40 204966.0 41 187248.0 42 168083.0 43 159765.5 44 148087.5 45 132979.5 46 124619.0 47 111097.5 48 92497.0 49 77664.5 50 64384.0 51 52707.5 52 43063.0 53 34721.0 54 28442.5 55 22495.0 56 16296.0 57 11053.5 58 7642.5 59 5666.5 60 4293.5 61 3217.0 62 2370.0 63 1748.0 64 1326.5 65 989.5 66 746.5 67 593.0 68 465.0 69 349.5 70 260.5 71 207.0 72 159.5 73 133.0 74 113.5 75 80.0 76 66.5 77 54.5 78 34.0 79 26.0 80 35.0 81 33.5 82 23.0 83 17.0 84 15.0 85 13.5 86 11.5 87 10.5 88 9.0 89 5.5 90 5.5 91 5.5 92 4.5 93 6.0 94 4.0 95 2.0 96 2.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2935270332488184 2 0.2705625074569256 3 0.2421747630333969 4 0.2297574624423409 5 0.23607831726128206 6 0.18610990283462286 7 0.1509524854985004 8 0.14919461463169426 9 0.12862378533928218 10-11 0.11390629201825642 12-13 0.13457062508017947 14-15 0.13724483288819306 16-17 0.13157850445582864 18-19 0.11876848803282657 20-21 0.12477143003543044 22-23 0.15600168905209244 24-25 0.5648001693540273 26-27 0.22440904682631377 28-29 0.19983625619883238 30-31 0.3003528084231188 32-33 0.3320318855334334 34-35 0.3396430923716259 36-37 0.33072283276027986 38-39 0.3384836456296899 40-41 0.3412139556994101 42-43 0.35228480200950824 44-45 0.33244330211928164 46-47 0.35264011633364983 48-49 0.3484324467056565 50-51 0.3540613736302165 52-53 0.33857714939920086 54-55 0.3315269651780742 56-57 0.3396430923716259 58-59 0.33255550664269484 60-61 0.30929176878836695 62-63 0.2954158093929399 64-65 0.30691677304278847 66-67 0.32303682290647867 68-69 0.30902995823373625 70-71 0.2898242839761842 72-73 0.28559791359428865 74-75 0.27750048715463915 76-77 0.28081052059532724 78-79 0.29002999226910836 80-81 0.2998104865599552 82-83 0.3218399746567383 84-85 0.31290101429149014 86-87 0.3157435288846234 88-89 0.32139115656308564 90-91 0.31471498742000287 92-93 0.29575242296317933 94-95 0.29212447670615393 96-97 0.309198265018856 98-99 0.31776321030606025 100-101 0.3184925397082458 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2673689.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.39875410743696 #Duplication Level Percentage of deduplicated Percentage of total 1 88.27791381564512 43.60818957696565 2 5.493908463416231 5.427844665461304 3 1.7014430029375065 2.5214749358978703 4 0.9986071021025649 1.9731978674681918 5 0.4892782519088066 1.2084868028079867 6 0.4465826946363675 1.3236377232587115 7 0.3309903606895644 1.144535800774496 8 0.27861089004749795 1.1010424679288404 9 0.18668980977268784 0.8300019606592669 >10 1.4666713161690696 14.179291660615135 >50 0.17931290176453366 6.108419632415366 >100 0.13418033207979935 13.885960892822283 >500 0.013200361610268425 4.2826030585242965 >1k 0.0024587854957820454 1.9398618537285368 >5k 1.5191172420568768E-4 0.4654511006721222 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6231 0.23304879512912685 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 6174 0.23091690918427685 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3010 0.11257853849120072 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 2917 0.10910019826539288 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 2818 0.10539744899275869 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.236211092613988E-4 0.0 0.0 2 5.9842412487017E-4 0.0 0.0 3 7.106286482833269E-4 0.0 0.0 4 7.854316638920982E-4 0.0 0.0 5 8.97636187305255E-4 0.0 0.0 6 0.001009840710718412 0.0 0.0 7 0.0010846437263271832 0.0 0.0 8 0.001309052773153497 0.0 0.0 9 0.0015708633277841963 0.0 0.0 10-11 0.002505901022893837 0.0 0.0 12-13 0.0036466470109275985 0.0 0.0 14-15 0.004619086213841625 0.0 0.0 16-17 0.0060029420026038935 0.0 0.0 18-19 0.007629907592094668 0.0 0.0 20-21 0.009518683736216142 0.0 0.0 22-23 0.011818876466185858 0.0 0.0 24-25 0.014436982012492851 0.0 0.0 26-27 0.017036386804897653 0.0 0.0 28-29 0.02034642024558578 0.0 0.0 30-31 0.029883804735704116 0.0 0.0 32-33 0.03338084571541417 0.0 0.0 34-35 0.03725190177316808 0.0 0.0 36-37 0.0418896887409119 0.0 0.0 38-39 0.04662097947816668 0.0 0.0 40-41 0.05206289886370479 0.0 0.0 42-43 0.05823414765142842 0.0 0.0 44-45 0.06395657834549942 0.0 0.0 46-47 0.0697164105473748 0.0 0.0 48-49 0.07614946988972913 0.0 0.0 50-51 0.08391028275913916 0.0 0.0 52-53 0.09217601598390837 0.0 0.0 54-55 0.1014702906732982 0.0 0.0 56-57 0.1118679098429174 0.0 0.0 58-59 0.12172320714937301 0.0 0.0 60-61 0.1337103904006786 0.0 0.0 62-63 0.14652040682368067 0.0 0.0 64-65 0.15901251043034548 0.0 0.0 66-67 0.17322508339601203 0.0 0.0 68-69 0.19108430337260618 0.0 0.0 70-71 0.2105143866769845 0.0 0.0 72-73 0.23198285215670184 0.0 0.0 74-75 0.2549473779485946 0.0 0.0 76-77 0.282530989954329 0.0 0.0 78-79 0.31213428338150023 0.0 1.8700753902192814E-5 80-81 0.3435141484293798 0.0 3.740150780438563E-5 82-83 0.3827296293622781 0.0 3.740150780438563E-5 84-85 0.42628368520048515 0.0 3.740150780438563E-5 86-87 0.47391450538937024 0.0 3.740150780438563E-5 88-89 0.5301663731271662 0.0 3.740150780438563E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 450 0.0 59.065983 2 GAGCGGC 340 0.0 58.561405 9 GGTACGG 70 1.3023964E-9 54.257496 1 AGAGCGG 385 0.0 51.71947 8 CGGGAGA 480 0.0 46.46446 4 CGGGTCG 65 2.5999543E-6 43.823364 1 ATTCGGA 120 1.8189894E-12 43.50843 2 GAACCGG 105 1.033186E-9 40.693123 1 TTGTGTT 33600 0.0 40.09968 1 CGGGTGC 95 1.9388608E-8 39.97921 1 CGGAAAG 285 0.0 39.960472 5 GGACGGG 250 0.0 39.87926 1 AACCGGA 120 8.731149E-11 39.553116 2 GAGAGCG 635 0.0 38.076218 7 CGTGCGG 75 6.950473E-6 37.980247 1 GTATGCG 75 6.950473E-6 37.980247 1 CGTCCGA 50 0.0016203319 37.980247 1 TCGGAAA 300 0.0 37.96245 4 ATGTCGC 80 1.0819893E-5 35.60648 1 GTCCGGC 95 8.7411354E-7 34.981808 1 >>END_MODULE