FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_35005-1.3420000003578b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_35005-1.3420000003578b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2673689
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG62310.23304879512912685No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC61740.23091690918427685No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA30100.11257853849120072No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA29170.10910019826539288No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA28180.10539744899275869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA4500.059.0659832
GAGCGGC3400.058.5614059
GGTACGG701.3023964E-954.2574961
AGAGCGG3850.051.719478
CGGGAGA4800.046.464464
CGGGTCG652.5999543E-643.8233641
ATTCGGA1201.8189894E-1243.508432
GAACCGG1051.033186E-940.6931231
TTGTGTT336000.040.099681
CGGGTGC951.9388608E-839.979211
CGGAAAG2850.039.9604725
GGACGGG2500.039.879261
AACCGGA1208.731149E-1139.5531162
GAGAGCG6350.038.0762187
CGTGCGG756.950473E-637.9802471
GTATGCG756.950473E-637.9802471
CGTCCGA500.001620331937.9802471
TCGGAAA3000.037.962454
ATGTCGC801.0819893E-535.606481
GTCCGGC958.7411354E-734.9818081
Produced by FastQC (version 0.11.2)