##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_34344-1.34200000035854.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4943093 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.627104122863965 33.0 33.0 33.0 27.0 33.0 2 31.49341050229077 33.0 33.0 33.0 27.0 33.0 3 31.423436904788154 33.0 33.0 33.0 27.0 33.0 4 35.052846062172 37.0 37.0 37.0 33.0 37.0 5 35.08303829201676 37.0 37.0 37.0 33.0 37.0 6 35.216376264820425 37.0 37.0 37.0 33.0 37.0 7 35.06078157946856 37.0 37.0 37.0 33.0 37.0 8 35.073834945043515 37.0 37.0 37.0 33.0 37.0 9 34.87154965524622 37.0 37.0 37.0 33.0 37.0 10-11 34.85112853430029 37.0 37.0 37.0 33.0 37.0 12-13 34.822865663259826 37.0 37.0 37.0 33.0 37.0 14-15 36.46713636583411 40.0 37.0 40.0 33.0 40.0 16-17 36.439029368858726 40.0 37.0 40.0 33.0 40.0 18-19 36.444095528852074 40.0 37.0 40.0 33.0 40.0 20-21 36.434865275648264 40.0 37.0 40.0 33.0 40.0 22-23 36.58544933303905 40.0 37.0 40.0 33.0 40.0 24-25 36.22740276179307 40.0 37.0 40.0 30.0 40.0 26-27 36.14697922940151 40.0 37.0 40.0 27.0 40.0 28-29 36.07114978415336 40.0 37.0 40.0 27.0 40.0 30-31 36.059221827305294 40.0 37.0 40.0 27.0 40.0 32-33 35.94925090424154 40.0 37.0 40.0 27.0 40.0 34-35 35.881523673538005 40.0 37.0 40.0 27.0 40.0 36-37 35.78899152817881 38.5 37.0 40.0 27.0 40.0 38-39 35.68202742695717 37.0 37.0 40.0 27.0 40.0 40-41 35.620373519171096 37.0 37.0 40.0 27.0 40.0 42-43 35.49190527064735 37.0 37.0 40.0 27.0 40.0 44-45 35.35733669182433 37.0 37.0 40.0 27.0 40.0 46-47 35.24662159906762 37.0 37.0 40.0 27.0 40.0 48-49 35.129289293161186 37.0 37.0 40.0 27.0 40.0 50-51 34.03805491824653 37.0 35.0 38.5 24.5 38.5 52-53 34.471889665033615 37.0 35.0 38.5 27.0 40.0 54-55 35.186532905611934 37.0 37.0 40.0 27.0 40.0 56-57 35.305987971498816 37.0 37.0 40.0 27.0 40.0 58-59 35.03364705054103 37.0 37.0 40.0 27.0 40.0 60-61 34.79881806795866 37.0 33.0 40.0 27.0 40.0 62-63 34.527915416521594 37.0 33.0 40.0 27.0 40.0 64-65 34.30098199649491 37.0 33.0 40.0 27.0 40.0 66-67 34.02905539102744 37.0 33.0 37.0 24.5 40.0 68-69 33.87189468213525 37.0 33.0 37.0 22.0 40.0 70-71 33.54631078152889 37.0 33.0 37.0 22.0 40.0 72-73 33.417553543904596 37.0 33.0 37.0 22.0 38.5 74-75 33.24770148973528 37.0 33.0 37.0 27.0 37.0 76-77 32.99398959315555 37.0 33.0 37.0 22.0 37.0 78-79 32.704775127637696 37.0 33.0 37.0 22.0 37.0 80-81 32.39416848924348 37.0 33.0 37.0 22.0 37.0 82-83 32.16969931579276 37.0 33.0 37.0 22.0 37.0 84-85 31.917989101155896 33.0 33.0 37.0 22.0 37.0 86-87 31.786382129569482 33.0 33.0 37.0 22.0 37.0 88-89 31.677678226972464 33.0 33.0 37.0 15.0 37.0 90-91 31.541768686124257 33.0 33.0 37.0 15.0 37.0 92-93 31.374346891713344 33.0 33.0 37.0 15.0 37.0 94-95 31.22379520272024 33.0 33.0 37.0 15.0 37.0 96-97 31.078205083335476 33.0 33.0 37.0 15.0 37.0 98-99 30.925852699918856 33.0 33.0 37.0 15.0 37.0 100-101 29.662725544512313 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.015491249578435173 2306 2 0.04502403797845034 2306 3 0.01558602518894503 2306 4 0.0870386064102533 2306 5 0.011416836972678368 2306 6 0.05618784981134439 2306 7 0.08370945805127405 2306 8 0.019732200331795013 2306 9 0.1380074534358613 2306 10-11 0.055810643834014684 2306 12-13 0.10658572599331251 2306 14-15 0.2657107435411561 2306 16-17 0.18182209048596576 2306 18-19 0.16896069838426087 2306 20-21 0.18012360320267362 2306 22-23 0.09130185782822053 2306 24-25 0.2203491369180952 2306 26-27 0.05000851223191205 2306 28-29 0.14099698635822477 2306 30-31 0.047889918021517985 2306 32-33 0.07952771380892187 2306 34-35 0.03854799742974535 2306 36-37 0.08086038339410351 2306 38-39 0.037244878146303506 2306 40-41 0.09927933744261708 2306 42-43 0.09401972901903832 2306 44-45 0.06727096184931014 2306 46-47 0.11029104230867404 2306 48-49 0.1642981688547991 2306 50-51 0.13971560892000667 2306 52-53 0.09276160665437061 2306 54-55 0.24662131599333037 2306 56-57 0.1341704804542374 2306 58-59 0.1364451337032122 2306 60-61 0.14496912647717153 2306 62-63 0.1867020490533875 2306 64-65 0.05084821357940683 2306 66-67 0.08595740032492927 2306 68-69 0.02899823675661395 2306 70-71 0.16777273122037428 2306 72-73 0.008690678201936919 2306 74-75 0.09623922569312526 2306 76-77 0.054584556501644954 2306 78-79 0.1691098525739747 2306 80-81 0.05796559230558529 2306 82-83 0.14878096567903043 2306 84-85 0.23485132163050437 2306 86-87 0.29341094978104465 2306 88-89 0.13711684724609086 2306 90-91 -0.07893121856675123 2306 92-93 -0.0415255930357219 2306 94-95 -0.13211326423268233 2306 96-97 0.03696030609991752 2306 98-99 0.06157577132631076 2306 100-101 0.1666460753015535 2307 1 0.03345947024437734 2307 2 0.04693987408033351 2307 3 0.05743818465738215 2307 4 0.11087443143794218 2307 5 0.11062364760390864 2307 6 -0.0449347969217726 2307 7 0.19597413136245478 2307 8 0.06712976400842763 2307 9 0.14867328953774717 2307 10-11 0.12205233818617245 2307 12-13 0.17198578136430598 2307 14-15 0.26611702814802385 2307 16-17 0.3295401137417713 2307 18-19 0.31288511698891597 2307 20-21 0.30187055779846617 2307 22-23 0.2560886795336472 2307 24-25 0.29136519450391773 2307 26-27 0.13286150226513627 2307 28-29 0.04536506498502746 2307 30-31 0.1212640874567299 2307 32-33 0.18860094193738774 2307 34-35 0.11237413253494566 2307 36-37 0.17934944762445326 2307 38-39 0.1600791804718824 2307 40-41 0.11572728871615823 2307 42-43 0.08749979546421827 2307 44-45 0.18460415675517794 2307 46-47 0.045022493028490373 2307 48-49 0.017990029319911116 2307 50-51 -0.17260305110213636 2307 52-53 -0.03530317739878086 2307 54-55 -0.016113318558169 2307 56-57 -0.08257508986690709 2307 58-59 0.08847932528681923 2307 60-61 0.09064880532545061 2307 62-63 0.07608673897586726 2307 64-65 0.18324024015748108 2307 66-67 0.11909624307133271 2307 68-69 -0.04897172448369247 2307 70-71 0.09231661272646363 2307 72-73 -0.06067670828754501 2307 74-75 0.05240824562667967 2307 76-77 0.05912636159578 2307 78-79 0.056438894655933325 2307 80-81 0.11721669972529725 2307 82-83 0.1032203344498015 2307 84-85 0.1553434312650559 2307 86-87 0.12875909485303083 2307 88-89 0.0959824341785378 2307 90-91 -0.06359345555456741 2307 92-93 0.15014522645486394 2307 94-95 0.17834562281050736 2307 96-97 0.1532864412051218 2307 98-99 -0.0803629053071333 2307 100-101 6.784673281288178E-4 2201 1 0.23627258179036303 2201 2 0.22161371621992743 2201 3 0.2156492087930637 2201 4 0.3226120374392494 2201 5 0.3012262242510104 2201 6 0.22591892127783808 2201 7 0.4223603022691762 2201 8 0.2570025624428709 2201 9 0.34266790793304835 2201 10-11 0.33922454953646053 2201 12-13 0.3373804323753973 2201 14-15 0.5253819498701304 2201 16-17 0.4763009584906328 2201 18-19 0.4534673087697314 2201 20-21 0.36546726360408144 2201 22-23 0.32951904483044814 2201 24-25 0.40744070004860333 2201 26-27 0.3690813326630433 2201 28-29 0.4193356292381196 2201 30-31 0.302601742381718 2201 32-33 0.36938820547293716 2201 34-35 0.35898616776000125 2201 36-37 0.24725347567402167 2201 38-39 0.3575820343531646 2201 40-41 0.3604231465113301 2201 42-43 0.36623022599259514 2201 44-45 0.341565588588189 2201 46-47 0.30196965545510324 2201 48-49 0.37224231242493744 2201 50-51 0.235803667749785 2201 52-53 0.3250441807225499 2201 54-55 0.45951249683648854 2201 56-57 0.471514114307908 2201 58-59 0.45496456664363905 2201 60-61 0.5067080029771134 2201 62-63 0.4078076293868307 2201 64-65 0.4378716817752206 2201 66-67 0.4293885620537239 2201 68-69 0.41945007916926613 2201 70-71 0.3561762833070219 2201 72-73 0.4486962532258332 2201 74-75 0.3554109364290312 2201 76-77 0.33947942747966664 2201 78-79 0.46907547325997 2201 80-81 0.4270218204037022 2201 82-83 0.3852327284484929 2201 84-85 0.4227500685775105 2201 86-87 0.3735548384929537 2201 88-89 0.40815831480475495 2201 90-91 0.3283096755608881 2201 92-93 0.3679004449806307 2201 94-95 0.39300845712231336 2201 96-97 0.36179916136167733 2201 98-99 0.4202832949537907 2201 100-101 0.3780104960300221 1101 1 0.029199963842302168 1101 2 0.047618764645495304 1101 3 0.09094317479316061 1101 4 0.09547971750037476 1101 5 -0.027640587822183704 1101 6 0.022788647045580035 1101 7 0.206949528832574 1101 8 0.04842255732396694 1101 9 0.1398223263254934 1101 10-11 0.13739289561141987 1101 12-13 0.1284504732524283 1101 14-15 0.2719926470795926 1101 16-17 0.22618156378239007 1101 18-19 0.22553573978880337 1101 20-21 0.2847382418338995 1101 22-23 0.18065204265305823 1101 24-25 0.16638985505863246 1101 26-27 0.16183402750593956 1101 28-29 0.15985592123816872 1101 30-31 0.14555098641614705 1101 32-33 0.09343283915605838 1101 34-35 0.13388877413489553 1101 36-37 0.03811301092954267 1101 38-39 0.07197922785882582 1101 40-41 0.07610551392988185 1101 42-43 0.104378736317571 1101 44-45 0.1992134803790222 1101 46-47 0.11565757484351025 1101 48-49 0.16348657684225287 1101 50-51 0.019933746313817835 1101 52-53 -0.06694557034295912 1101 54-55 0.2118168147055357 1101 56-57 0.1590231558489208 1101 58-59 0.27913696425630263 1101 60-61 0.19910133133991792 1101 62-63 0.21178180396368163 1101 64-65 0.24112774934474857 1101 66-67 0.2994336816617178 1101 68-69 0.25747713145997864 1101 70-71 0.21773765543274237 1101 72-73 0.0195965403295304 1101 74-75 0.12587820326854882 1101 76-77 0.2366956529191313 1101 78-79 0.30230111952150907 1101 80-81 0.28956327239173163 1101 82-83 0.2018840708623202 1101 84-85 0.395260770052726 1101 86-87 0.33979650636515757 1101 88-89 0.2842690333429765 1101 90-91 0.1991410614853919 1101 92-93 0.1307593709753867 1101 94-95 0.20964820781688687 1101 96-97 0.15129377167589908 1101 98-99 0.2228082727904237 1101 100-101 0.17206424544116317 2304 1 0.17575355003845772 2304 2 0.04700422414468264 2304 3 0.18705530177449958 2304 4 0.2238007506142594 2304 5 0.1366773799076455 2304 6 0.19895999072301862 2304 7 0.2695584299467555 2304 8 0.20664070351936914 2304 9 0.3001935598080152 2304 10-11 0.22907453895837193 2304 12-13 0.25095135407987357 2304 14-15 0.39687233702833424 2304 16-17 0.36076196058861854 2304 18-19 0.3736878840416793 2304 20-21 0.2576459760738814 2304 22-23 0.14818970913467666 2304 24-25 0.20447652011524298 2304 26-27 0.25983222298585673 2304 28-29 0.22250068274563972 2304 30-31 0.2738340681517073 2304 32-33 0.2216808968923445 2304 34-35 0.21202020718102688 2304 36-37 0.29655102420102963 2304 38-39 0.14065752667522702 2304 40-41 0.21561467610354157 2304 42-43 0.1983266937911239 2304 44-45 0.18682021209623656 2304 46-47 0.13261901812501975 2304 48-49 0.18844530602519427 2304 50-51 0.1951656104165167 2304 52-53 0.3363023567067529 2304 54-55 0.3943636762938283 2304 56-57 0.33868456264274016 2304 58-59 0.23120374613623795 2304 60-61 0.32999082591747 2304 62-63 0.31840897192309825 2304 64-65 0.4292505361677783 2304 66-67 0.5242884888885797 2304 68-69 0.37754452390755233 2304 70-71 0.41040300268785046 2304 72-73 0.40918333032249166 2304 74-75 0.5525248801183196 2304 76-77 0.46515113637055094 2304 78-79 0.3662563013483364 2304 80-81 0.46476335352195264 2304 82-83 0.43889440137386515 2304 84-85 0.4008188173654439 2304 86-87 0.36982650154543606 2304 88-89 0.3136988112699193 2304 90-91 0.25011310815199295 2304 92-93 0.2531294606890988 2304 94-95 0.23720582229570653 2304 96-97 0.2636628890815693 2304 98-99 0.2915281822089497 2304 100-101 0.3763943617940271 2202 1 0.042704307081489645 2202 2 -0.03619225200953835 2202 3 -0.01239489427598528 2202 4 0.08062757674657206 2202 5 0.03282250164771483 2202 6 0.11429862082609787 2202 7 0.15845071500222474 2202 8 0.1701062435754892 2202 9 0.11032524147928058 2202 10-11 0.10352146906338788 2202 12-13 0.1389945870721192 2202 14-15 0.20074671720188775 2202 16-17 0.11232048851917398 2202 18-19 0.1641711377109445 2202 20-21 0.15165923237466217 2202 22-23 0.13470491718946676 2202 24-25 0.08149939048439592 2202 26-27 0.13406628845353197 2202 28-29 0.050540152868641997 2202 30-31 0.11219248832168915 2202 32-33 -0.02961994950320701 2202 34-35 0.00860959126433869 2202 36-37 0.10464569349044694 2202 38-39 0.03771142508900738 2202 40-41 -0.01128449462758141 2202 42-43 -0.03140241087727702 2202 44-45 -0.022137948173245547 2202 46-47 0.02725814833966922 2202 48-49 0.12208485690612036 2202 50-51 -0.02032053416141366 2202 52-53 0.012046726623047732 2202 54-55 0.21340307578490325 2202 56-57 0.25927211404622597 2202 58-59 0.21630150260359926 2202 60-61 0.2138149574103707 2202 62-63 0.22586160946315914 2202 64-65 0.23730717047501315 2202 66-67 0.19268295009508307 2202 68-69 0.1578646240673791 2202 70-71 0.30798179635698375 2202 72-73 0.16374265270600574 2202 74-75 0.17983971663664988 2202 76-77 0.07805530884247247 2202 78-79 0.15326980829870962 2202 80-81 0.09355417862166604 2202 82-83 0.18763790307996686 2202 84-85 0.32771942573763724 2202 86-87 0.24287923234464515 2202 88-89 0.12639790111810711 2202 90-91 0.1155341838450461 2202 92-93 0.16518561244899033 2202 94-95 0.09779370498169371 2202 96-97 0.20209394137857473 2202 98-99 0.12826103662029098 2202 100-101 0.16560639697497948 1102 1 0.026081944085444064 1102 2 -0.05614155035787505 1102 3 0.015292662675179258 1102 4 -0.0037116211192156356 1102 5 -0.047485213802687554 1102 6 0.02964059627799287 1102 7 0.06799168667146915 1102 8 0.18784372341585254 1102 9 0.06201013066981176 1102 10-11 0.019750923102094475 1102 12-13 -0.005632695217741457 1102 14-15 0.03543813313181232 1102 16-17 0.07091983431433846 1102 18-19 0.09230589512200282 1102 20-21 0.07615077925205327 1102 22-23 0.0866182211812827 1102 24-25 0.04392643142444541 1102 26-27 0.16259689459474913 1102 28-29 0.11945541663504855 1102 30-31 0.06538886448037573 1102 32-33 0.06385661695750855 1102 34-35 -0.08111876485241964 1102 36-37 0.14527845684805385 1102 38-39 0.03361970506454526 1102 40-41 -0.06266983760810518 1102 42-43 -0.003465583378044812 1102 44-45 0.009396883919137622 1102 46-47 -0.06247359128919783 1102 48-49 0.13985789135737292 1102 50-51 0.058043825053530895 1102 52-53 0.1141450920871776 1102 54-55 0.23853036794795202 1102 56-57 0.3452364516066879 1102 58-59 0.30486149316082134 1102 60-61 0.2435076612935987 1102 62-63 0.300933034849173 1102 64-65 0.2829063194717989 1102 66-67 0.30915170164124106 1102 68-69 0.2763818425941196 1102 70-71 0.26595380572609173 1102 72-73 0.15313545316909227 1102 74-75 0.25096957228159766 1102 76-77 0.19232006278570424 1102 78-79 0.26865589143636726 1102 80-81 0.267585290514738 1102 82-83 0.33574265927740754 1102 84-85 0.3940903993304907 1102 86-87 0.3681711850660001 1102 88-89 0.32810714050236456 1102 90-91 0.1714083935176305 1102 92-93 0.2657017699950792 1102 94-95 0.22294327285727533 1102 96-97 0.2974382065252321 1102 98-99 0.2308475707745714 1102 100-101 0.21663120177960948 2305 1 0.12800073535650824 2305 2 0.06440002264284317 2305 3 0.05261940355907413 2305 4 0.059178085204194986 2305 5 0.06315616465414564 2305 6 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0.20681654214901357 1213 68-69 0.15500587600548243 1213 70-71 0.1782437395303873 1213 72-73 0.22560780447921758 1213 74-75 0.24687035987151518 1213 76-77 0.2129873331614789 1213 78-79 0.18559317941427622 1213 80-81 0.2277675277679947 1213 82-83 0.2839861832924484 1213 84-85 0.3169586295733673 1213 86-87 0.2989647416624983 1213 88-89 0.22274497101522783 1213 90-91 0.31205740841831897 1213 92-93 0.3398336308904284 1213 94-95 0.34366130172640297 1213 96-97 0.18609557274919908 1213 98-99 0.13614160162508426 1213 100-101 0.2709298715725055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2517.0 3 585.0 4 573.0 5 580.0 6 1555.0 7 6345.0 8 21185.0 9 43569.0 10 53541.0 11 39510.0 12 25302.0 13 17463.0 14 13728.0 15 11961.0 16 11679.0 17 12426.0 18 13399.0 19 15301.0 20 17147.0 21 19292.0 22 21719.0 23 24298.0 24 27387.0 25 31549.0 26 37009.0 27 44215.0 28 53628.0 29 66095.0 30 81587.0 31 104208.0 32 141578.0 33 201371.0 34 304168.0 35 506454.0 36 962435.0 37 1564276.0 38 443080.0 39 378.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.929230274734905 24.15914787374272 26.268481782822413 14.643140068699962 2 29.82295874284863 22.739401493476866 34.829325768775014 12.608313994899481 3 38.532906813294595 19.625972239151107 26.42176444712242 15.41935650043188 4 28.194558058742725 18.602194669994457 36.79862237959915 16.40462489166367 5 32.67792624343139 17.54663121228984 33.818498845677745 15.956943698601023 6 25.26566299909233 18.34411471274181 40.91666251140669 15.473559776759165 7 33.044199785013994 18.535319201532662 31.818698514043493 16.601782499409854 8 27.8388334795928 27.892204171059348 27.984194656730615 16.284767692617237 9 31.615727034826836 25.577742817287046 25.192945579805066 17.613584568081052 10-11 25.307518530712557 28.691288268727966 28.878289188187132 17.12290401237234 12-13 26.56663411708934 25.203907570005228 28.829126874470735 19.400331438434705 14-15 24.61654798727896 25.668459815221787 30.08888418414611 19.626108013353143 16-17 23.798672039069615 26.86512103556278 31.26910307483599 18.067103850531616 18-19 23.09158281697099 27.26955263766368 31.32604847271833 18.312816072646992 20-21 22.788468453138787 28.019930943605292 30.704207092207202 18.48739351104872 22-23 23.827004882441376 27.25952600876957 30.271734290470565 18.641734818318493 24-25 22.744740737138642 28.118916051699465 30.23287587756114 18.903467333600755 26-27 22.060306958627802 28.882112193287217 29.93005787789174 19.12752297019324 28-29 21.99053869595517 28.617596776880983 29.66930435618092 19.722560170982927 30-31 22.55616793139118 28.189203842425027 29.68828690193424 19.566341324249557 32-33 22.560138834621377 27.760033287833195 30.419041046537 19.260786831008428 34-35 21.782073506903703 28.00738479276704 30.21091723267172 19.999624467657533 36-37 20.698551660369617 28.483901096040526 30.2898982996905 20.527648943899358 38-39 21.07593639116914 28.609713865815735 30.388116175025782 19.926233567989346 40-41 21.410558303915618 28.04270863571497 30.754257364240374 19.792475696129042 42-43 21.90032254708147 28.14008339022106 30.922230878744124 19.037363183953346 44-45 21.184641654678266 28.74469656028072 31.043759562176028 19.026902222864987 46-47 21.83497959610013 28.87208930635876 30.09079103545998 19.202140062081128 48-49 21.2201697326113 28.959359244014156 30.112074032952773 19.70839699042177 50-51 20.78121989551224 28.465910325195974 30.381512492099873 20.371357287191916 52-53 22.114037844131676 28.08027587371648 29.628980056480387 20.176706225671456 54-55 22.94061440236103 28.30249515010938 29.104976345524324 19.651914102005264 56-57 23.17722553485874 28.273471079647944 28.707259106006312 19.84204427948701 58-59 22.551548366596066 28.152615377620478 29.18147682014558 20.11435943563788 60-61 21.23995935291381 28.456602944630777 29.043043194377997 21.260394508077415 62-63 21.927516178255992 28.45006185005574 29.0758872234007 20.54653474828757 64-65 21.374139364463435 28.74776857236725 29.63153262678476 20.246559436384555 66-67 21.2227728132577 28.29949973914832 29.316217485251812 21.16150996234217 68-69 21.278424495189732 27.960484127576812 30.53420274232354 20.226888634909916 70-71 21.05077565462978 29.569879928744463 29.624893989133128 19.754450427492625 72-73 20.69344838699304 31.237131504673464 28.402134208598124 19.667285899735372 74-75 21.165684994062087 30.88076461320801 28.14994374502723 19.803606647702676 76-77 21.23997170761465 30.335924908528018 28.51896179158599 19.90514159227135 78-79 21.07574501963417 30.140210167649013 29.014302628064414 19.769742184652408 80-81 20.547791635013084 30.45011801105116 28.87176078332067 20.130329570615086 82-83 19.85951128509668 30.346400618522157 29.188711260295726 20.605376836085437 84-85 19.934887826854585 29.90609113877812 29.26652664048786 20.892494393879435 86-87 19.965150815770656 29.98720349806906 29.819472012129435 20.228173674030852 88-89 20.123951418772286 30.231490041205518 29.544291717920718 20.100266822101485 90-91 20.329143804807327 29.825282190955726 29.268419148214658 20.57715485602229 92-93 19.981075496938132 30.588911787305385 29.029207688863075 20.40080502689341 94-95 19.67222560941588 31.010084819612494 29.047425332669196 20.27026423830243 96-97 20.062503874657825 31.137465604960536 28.79356523209036 20.006465288291285 98-99 20.48787754222751 30.413030443462034 28.408218822511582 20.690873191798875 100-101 20.501662592337066 30.342469972219604 27.906050975611763 21.249816459831568 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1423.0 1 1160.0 2 681.5 3 538.5 4 645.0 5 719.0 6 848.5 7 1103.5 8 1400.0 9 1703.5 10 1947.5 11 2051.0 12 2011.5 13 1803.0 14 1568.5 15 1475.5 16 1428.5 17 1349.0 18 1434.0 19 1649.5 20 1931.0 21 2345.5 22 3031.0 23 3955.0 24 5162.0 25 6997.5 26 10076.0 27 14485.0 28 19004.5 29 23574.0 30 29292.5 31 36415.5 32 49345.5 33 71834.0 34 104648.0 35 144518.5 36 197304.5 37 261776.5 38 319821.0 39 344609.0 40 339559.5 41 314611.0 42 289482.5 43 290976.0 44 286881.5 45 267694.0 46 252864.5 47 224214.0 48 181333.0 49 151373.5 50 129220.5 51 110427.0 52 94558.0 53 77775.5 54 61184.0 55 46304.5 56 35293.5 57 26983.0 58 21058.0 59 16456.0 60 12432.0 61 9240.0 62 6915.0 63 5193.0 64 3799.5 65 2719.5 66 1952.0 67 1411.0 68 1092.5 69 811.0 70 590.5 71 448.5 72 346.5 73 278.5 74 222.5 75 175.0 76 144.0 77 123.5 78 98.5 79 82.5 80 67.5 81 53.5 82 42.5 83 36.5 84 38.0 85 32.0 86 25.0 87 19.5 88 17.0 89 17.5 90 12.5 91 10.5 92 13.0 93 8.0 94 5.5 95 5.5 96 3.0 97 2.0 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3033930375171982 2 0.2789751275163142 3 0.24956034612336853 4 0.23651183580806592 5 0.2439970277718829 6 0.19394739285706336 7 0.15957620056915783 8 0.1577959386966824 9 0.13608888200161315 10-11 0.12040032425042378 12-13 0.1422793380581753 14-15 0.1451621484766724 16-17 0.13915376465706797 18-19 0.12610525434176537 20-21 0.1324676675110098 22-23 0.16102266334054408 24-25 0.5717978601656898 26-27 0.22572911333045928 28-29 0.19694146964259018 30-31 0.29833547537948407 32-33 0.3311287082804228 34-35 0.33891735397250267 36-37 0.33002615973440114 38-39 0.3385127490014855 40-41 0.3423564962261483 42-43 0.35312910357947946 44-45 0.33289885502862276 46-47 0.3528458800997675 48-49 0.3497101915743847 50-51 0.35483855958202687 52-53 0.3394838009319266 54-55 0.33249425005760563 56-57 0.3406571553478763 58-59 0.3324537895605039 60-61 0.3097352204378918 62-63 0.2962011841573687 64-65 0.307378396481717 66-67 0.32294557274160124 68-69 0.3095430330766587 70-71 0.2903647574504465 72-73 0.2861872111246946 74-75 0.2781861478228308 76-77 0.2802192878021919 78-79 0.2895656626326877 80-81 0.2982140938881789 82-83 0.32094277813506644 84-85 0.31241572837088033 86-87 0.315076006055318 88-89 0.3204471370455705 90-91 0.3133665500527706 92-93 0.29459287939757556 94-95 0.29127511863523503 96-97 0.30827864254223014 98-99 0.3165528141995305 100-101 0.3175643266270734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4943093.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.140510397173976 #Duplication Level Percentage of deduplicated Percentage of total 1 92.27191433721622 48.11104708866768 2 3.7430003781051027 3.903239002624305 3 1.177441452808369 1.841771949366552 4 0.6106834941676638 1.2736539630812644 5 0.3088363307803271 0.8051441958038353 6 0.1967758429106407 0.6155995729916961 7 0.18109591104974837 0.6609703263082569 8 0.18010828854805216 0.751275047332554 9 0.1434999387554834 0.6733944043806699 >10 0.9166407741623396 9.509616567602619 >50 0.11806970761499562 4.262600691486148 >100 0.12152889775789906 13.294212764660571 >500 0.022717155337585237 8.085729095965862 >1k 0.00757093746855048 5.736167913053677 >5k 1.1655331714230185E-4 0.4755774166742593 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8827 0.17857240395841226 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7575 0.15324413277273966 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 7075 0.1431290084973113 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0015779593869668241 0.0 0.0 2 0.0016993408782719646 0.0 0.0 3 0.0017600316239245347 0.0 0.0 4 0.001881413115229675 0.0 0.0 5 0.0020837156007382424 0.0 0.0 6 0.0023467088318993795 0.0 0.0 7 0.0028322347971199407 0.0 0.0 8 0.0031963792710353617 0.0 0.0 9 0.0034391422536456423 0.0 0.0 10-11 0.005168828504743893 0.0 2.023024855085672E-5 12-13 0.007242428981206707 0.0 2.023024855085672E-5 14-15 0.00949810169462723 0.0 2.023024855085672E-5 16-17 0.011915616396454609 1.011512427542836E-5 3.0345372826285078E-5 18-19 0.01499061417618483 2.023024855085672E-5 5.05756213771418E-5 20-21 0.018500562299758473 2.023024855085672E-5 6.069074565257016E-5 22-23 0.022556727134205243 2.023024855085672E-5 6.069074565257016E-5 24-25 0.026946691069741152 4.046049710171344E-5 6.069074565257016E-5 26-27 0.031721029727743336 4.046049710171344E-5 6.069074565257016E-5 28-29 0.03694043385386438 4.046049710171344E-5 6.069074565257016E-5 30-31 0.05652331445109368 4.046049710171344E-5 6.069074565257016E-5 32-33 0.06368482243809696 4.046049710171344E-5 6.069074565257016E-5 34-35 0.0698752784946591 4.046049710171344E-5 6.069074565257016E-5 36-37 0.0770367864816624 4.046049710171344E-5 7.080586992799852E-5 38-39 0.08483554729801765 6.069074565257016E-5 9.103611847885524E-5 40-41 0.09296810721546206 6.069074565257016E-5 1.011512427542836E-4 42-43 0.10166711409233045 6.069074565257016E-5 1.011512427542836E-4 44-45 0.11204523159891995 6.069074565257016E-5 1.011512427542836E-4 46-47 0.12255484572109002 6.069074565257016E-5 1.1126636702971196E-4 48-49 0.13418723863783263 6.069074565257016E-5 1.4161173985599703E-4 50-51 0.14811576476509747 6.069074565257016E-5 1.4161173985599703E-4 52-53 0.16240843536627775 6.069074565257016E-5 1.4161173985599703E-4 54-55 0.17749008566094143 6.069074565257016E-5 1.4161173985599703E-4 56-57 0.19399796847844053 6.069074565257016E-5 1.4161173985599703E-4 58-59 0.2115477090963087 6.069074565257016E-5 1.517268641314254E-4 60-61 0.23005838652034263 6.069074565257016E-5 1.719571126822821E-4 62-63 0.2507438156635936 6.069074565257016E-5 1.8207223695771048E-4 64-65 0.272997089069536 6.069074565257016E-5 1.8207223695771048E-4 66-67 0.2966462496254875 6.069074565257016E-5 1.8207223695771048E-4 68-69 0.32280396100174524 6.069074565257016E-5 2.023024855085672E-4 70-71 0.3517129861809195 6.069074565257016E-5 2.023024855085672E-4 72-73 0.38400046286808687 6.069074565257016E-5 2.023024855085672E-4 74-75 0.4181187770491067 6.069074565257016E-5 2.023024855085672E-4 76-77 0.4566675156627642 6.069074565257016E-5 2.326478583348523E-4 78-79 0.4995758728391313 6.069074565257016E-5 2.4276298261028065E-4 80-81 0.5467022368383521 6.069074565257016E-5 2.4276298261028065E-4 82-83 0.598319716015863 6.069074565257016E-5 2.4276298261028065E-4 84-85 0.6546609582299989 6.069074565257016E-5 2.6299323116113737E-4 86-87 0.7147346813017679 6.069074565257016E-5 2.6299323116113737E-4 88-89 0.7822531358402522 6.069074565257016E-5 2.731083554365657E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1460 0.0 72.10273 9 CGGGAGA 2050 0.0 68.759514 4 AAGCGGC 665 0.0 66.31518 9 AGAGCGG 1765 0.0 62.870193 8 CGGAAAG 1230 0.0 62.1228 5 GAGAGCG 2065 0.0 59.938694 7 ATTCGGA 540 0.0 58.02171 2 GTTCGGA 460 0.0 55.72836 2 TCGGAGA 750 0.0 52.522705 4 TCGGGAG 2495 0.0 52.313572 3 GATCCGG 500 0.0 51.27685 1 TTGTGTT 67420 0.0 50.84474 1 CGGAGAG 1210 0.0 49.42144 5 GGATCGG 940 0.0 48.993843 1 TCGGGAA 855 0.0 48.29536 3 TCGGAAA 1070 0.0 47.903828 4 GGTTCGG 690 0.0 46.790474 1 AAAGCGG 1030 0.0 46.50052 8 GGTCCGG 445 0.0 44.81123 1 TTCGGAG 605 0.0 43.932396 3 >>END_MODULE