FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_34344-1.34200000035854.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_34344-1.34200000035854.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4943093
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC88270.17857240395841226No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG75750.15324413277273966No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA70750.1431290084973113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC14600.072.102739
CGGGAGA20500.068.7595144
AAGCGGC6650.066.315189
AGAGCGG17650.062.8701938
CGGAAAG12300.062.12285
GAGAGCG20650.059.9386947
ATTCGGA5400.058.021712
GTTCGGA4600.055.728362
TCGGAGA7500.052.5227054
TCGGGAG24950.052.3135723
GATCCGG5000.051.276851
TTGTGTT674200.050.844741
CGGAGAG12100.049.421445
GGATCGG9400.048.9938431
TCGGGAA8550.048.295363
TCGGAAA10700.047.9038284
GGTTCGG6900.046.7904741
AAAGCGG10300.046.500528
GGTCCGG4450.044.811231
TTCGGAG6050.043.9323963
Produced by FastQC (version 0.11.2)