##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_34311-1.34200000035761.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2694502 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.899807830909015 33.0 33.0 33.0 27.0 33.0 2 31.867115333371437 33.0 33.0 33.0 33.0 33.0 3 31.840020159569374 33.0 33.0 33.0 33.0 33.0 4 35.562198135314055 37.0 37.0 37.0 33.0 37.0 5 35.59179061659631 37.0 37.0 37.0 33.0 37.0 6 35.604990087222056 37.0 37.0 37.0 33.0 37.0 7 35.54577395006573 37.0 37.0 37.0 33.0 37.0 8 35.46068624183616 37.0 37.0 37.0 33.0 37.0 9 35.4063422480295 37.0 37.0 37.0 33.0 37.0 10-11 35.360675738967714 37.0 37.0 37.0 33.0 37.0 12-13 35.34457313447902 37.0 37.0 37.0 33.0 37.0 14-15 36.973872351922545 40.0 37.0 40.0 33.0 40.0 16-17 36.96168123089164 40.0 37.0 40.0 33.0 40.0 18-19 36.958522391150574 40.0 37.0 40.0 33.0 40.0 20-21 36.95692859014393 40.0 37.0 40.0 33.0 40.0 22-23 36.936359111999174 40.0 37.0 40.0 33.0 40.0 24-25 36.67762837065996 40.0 37.0 40.0 33.0 40.0 26-27 36.59439759183701 40.0 37.0 40.0 33.0 40.0 28-29 36.58512444971279 40.0 37.0 40.0 33.0 40.0 30-31 36.59380044995328 40.0 37.0 40.0 33.0 40.0 32-33 36.47770422883338 40.0 37.0 40.0 33.0 40.0 34-35 36.4069655543028 40.0 37.0 40.0 33.0 40.0 36-37 36.32594019228785 37.0 37.0 40.0 33.0 40.0 38-39 36.2821513956939 37.0 37.0 40.0 33.0 40.0 40-41 36.173584395186936 37.0 37.0 40.0 33.0 40.0 42-43 36.05538166236285 37.0 37.0 40.0 33.0 40.0 44-45 35.96133979488603 37.0 37.0 40.0 30.0 40.0 46-47 35.872721193007095 37.0 37.0 40.0 27.0 40.0 48-49 35.754464461336454 37.0 37.0 40.0 27.0 40.0 50-51 34.59634377706901 37.0 35.0 38.5 27.0 38.5 52-53 35.07997414735635 37.0 35.0 38.5 27.0 40.0 54-55 35.858159689619825 37.0 37.0 40.0 27.0 40.0 56-57 35.92837284960264 37.0 37.0 40.0 27.0 40.0 58-59 35.68776809221147 37.0 37.0 40.0 27.0 40.0 60-61 35.43833869857956 37.0 37.0 40.0 27.0 40.0 62-63 35.19683989843021 37.0 35.0 40.0 27.0 40.0 64-65 34.95644148714679 37.0 33.0 40.0 27.0 40.0 66-67 34.68052686544675 37.0 33.0 37.0 27.0 40.0 68-69 34.46689648031436 37.0 33.0 37.0 27.0 40.0 70-71 34.134389582935924 37.0 33.0 37.0 27.0 40.0 72-73 33.8917454876634 37.0 33.0 37.0 27.0 38.5 74-75 33.64724594748863 37.0 33.0 37.0 27.0 37.0 76-77 33.40315000694005 37.0 33.0 37.0 27.0 37.0 78-79 33.15999561328958 37.0 33.0 37.0 27.0 37.0 80-81 32.94373412972045 37.0 33.0 37.0 27.0 37.0 82-83 32.78788919065564 37.0 33.0 37.0 27.0 37.0 84-85 32.55105247648731 35.0 33.0 37.0 27.0 37.0 86-87 32.45073727909647 33.0 33.0 37.0 22.0 37.0 88-89 32.371754780660766 33.0 33.0 37.0 22.0 37.0 90-91 32.25042549606569 33.0 33.0 37.0 22.0 37.0 92-93 32.09124153554163 33.0 33.0 37.0 22.0 37.0 94-95 31.956014135450634 33.0 33.0 37.0 22.0 37.0 96-97 31.815583918661034 33.0 33.0 37.0 22.0 37.0 98-99 31.670599613583512 33.0 33.0 37.0 22.0 37.0 100-101 30.404789827582242 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.18061614854974906 2306 2 0.2199756582369261 2306 3 0.23549450682875062 2306 4 0.3962435562361435 2306 5 0.38103856825664195 2306 6 0.4273985253404362 2306 7 0.41249229428621703 2306 8 0.5604970177346686 2306 9 0.5373243442265192 2306 10-11 0.4415947660361823 2306 12-13 0.5108393313103363 2306 14-15 0.6399642057960975 2306 16-17 0.6132012800461126 2306 18-19 0.5387105371725553 2306 20-21 0.6661948703619345 2306 22-23 0.5995719069060499 2306 24-25 0.6332937384108277 2306 26-27 0.43636313901499335 2306 28-29 0.4685127601523149 2306 30-31 0.4563486850062546 2306 32-33 0.4963453745706019 2306 34-35 0.4007816697403328 2306 36-37 0.390787819887052 2306 38-39 0.4566206098903294 2306 40-41 0.45466896668288825 2306 42-43 0.5226561407321952 2306 44-45 0.7182066514685275 2306 46-47 0.5289570919694597 2306 48-49 0.632280903835472 2306 50-51 0.4565902011995391 2306 52-53 0.45745020792112 2306 54-55 0.6578850897536839 2306 56-57 0.5978116478243507 2306 58-59 0.6013482049248253 2306 60-61 0.6383744375415787 2306 62-63 0.6447133611160965 2306 64-65 0.5648060039249998 2306 66-67 0.649695511269158 2306 68-69 0.516051570853783 2306 70-71 0.5385621474746642 2306 72-73 0.36027374796203304 2306 74-75 0.45834394143962953 2306 76-77 0.6069054727073251 2306 78-79 0.5628603206647043 2306 80-81 0.5822213231654771 2306 82-83 0.46420275734089245 2306 84-85 0.6767858714137134 2306 86-87 0.7280783403659825 2306 88-89 0.713927169995273 2306 90-91 0.4897941561450878 2306 92-93 0.496942089442598 2306 94-95 0.28658599409196484 2306 96-97 0.3974148866307168 2306 98-99 0.4841687146460778 2306 100-101 0.46921945435958534 2307 1 0.06691124083489441 2307 2 0.04309450243904678 2307 3 -0.041677319083873954 2307 4 -0.026869514749790824 2307 5 -0.064371003226384 2307 6 -0.0372751931399975 2307 7 0.11917017910433003 2307 8 0.012313161175320886 2307 9 0.06858418734785943 2307 10-11 0.10149559123819074 2307 12-13 0.06568247953568118 2307 14-15 0.19684467673206285 2307 16-17 0.16355419618533773 2307 18-19 0.2851680039928297 2307 20-21 0.19376310963571086 2307 22-23 0.25766124403045865 2307 24-25 0.06237246611850367 2307 26-27 -0.021121085813284424 2307 28-29 -0.01699802373441628 2307 30-31 -0.03301020216670025 2307 32-33 0.04454451317278796 2307 34-35 -0.07550725029751248 2307 36-37 0.04357839165902533 2307 38-39 0.0427903894242192 2307 40-41 0.07458687381278395 2307 42-43 0.02828262100322121 2307 44-45 0.14816650214599036 2307 46-47 0.05644198750962914 2307 48-49 0.006940418506090396 2307 50-51 0.06560790827617069 2307 52-53 -0.18605114581317395 2307 54-55 0.0073016411682758076 2307 56-57 -0.06500125554021707 2307 58-59 0.14252637684724334 2307 60-61 0.08955892321169046 2307 62-63 0.0894607107429195 2307 64-65 0.10481467506929931 2307 66-67 0.17223652445161264 2307 68-69 0.15922917168060025 2307 70-71 0.04113547368769588 2307 72-73 0.024084087622412653 2307 74-75 0.2669288554031439 2307 76-77 0.2473107977634541 2307 78-79 0.29330786660917596 2307 80-81 0.14912072968450474 2307 82-83 0.2612753405353416 2307 84-85 0.3745744183150137 2307 86-87 0.2987811360459176 2307 88-89 0.14843102290956978 2307 90-91 0.053872610757004225 2307 92-93 0.22616862846458474 2307 94-95 0.11603174552841011 2307 96-97 0.23842461648098734 2307 98-99 0.18493867490427718 2307 100-101 0.11853505939122755 2201 1 0.25860340215939814 2201 2 0.23100722963352638 2201 3 0.2646389775932505 2201 4 0.33632349998293165 2201 5 0.24311262985649762 2201 6 0.2868531662182505 2201 7 0.31993206015282993 2201 8 0.18618711189819948 2201 9 0.32736706815581584 2201 10-11 0.2951347221374121 2201 12-13 0.32740598756986117 2201 14-15 0.5009446480673816 2201 16-17 0.431699591431304 2201 18-19 0.435601086567857 2201 20-21 0.37765509682036225 2201 22-23 0.4583489657878701 2201 24-25 0.4793643962043532 2201 26-27 0.5311266323475934 2201 28-29 0.4712194866190629 2201 30-31 0.37282863163425617 2201 32-33 0.42739358249290405 2201 34-35 0.3086736123018383 2201 36-37 0.29038012813916936 2201 38-39 0.3836733691368508 2201 40-41 0.36513798109169215 2201 42-43 0.3658084518754592 2201 44-45 0.3199125038843107 2201 46-47 0.17457351150995493 2201 48-49 0.3117093604412915 2201 50-51 0.2891439923428365 2201 52-53 0.33000952801427985 2201 54-55 0.3736516130799643 2201 56-57 0.3877704191456459 2201 58-59 0.48093520280191626 2201 60-61 0.45720918031003777 2201 62-63 0.3580399186294727 2201 64-65 0.37870530202654606 2201 66-67 0.3552866307722127 2201 68-69 0.3767933194673887 2201 70-71 0.35128884124957693 2201 72-73 0.346011491440116 2201 74-75 0.18134442255266237 2201 76-77 0.2784849814156658 2201 78-79 0.3366403315487432 2201 80-81 0.34838636058027106 2201 82-83 0.21982913445550167 2201 84-85 0.24994262111646748 2201 86-87 0.32122450409194414 2201 88-89 0.4377517775975548 2201 90-91 0.38302804786261646 2201 92-93 0.1916849423558915 2201 94-95 0.23613867323577864 2201 96-97 0.22795393937208885 2201 98-99 0.33966454297144466 2201 100-101 0.21729717559768602 1101 1 0.2473618884796842 1101 2 0.18845784607187 1101 3 0.21908493187105194 1101 4 0.28300135081413913 1101 5 0.31501438580063024 1101 6 0.36901955273977904 1101 7 0.37493161759059035 1101 8 0.33863411317654624 1101 9 0.27310807207742016 1101 10-11 0.2909207505449274 1101 12-13 0.3070972500928022 1101 14-15 0.3773256532528322 1101 16-17 0.24972124516843763 1101 18-19 0.3096436534807765 1101 20-21 0.392519453117373 1101 22-23 0.3582650845538353 1101 24-25 0.38589157244734906 1101 26-27 0.38857776817437895 1101 28-29 0.30889339247860903 1101 30-31 0.30495765464944213 1101 32-33 0.2517783939300031 1101 34-35 0.38595017301543066 1101 36-37 0.35868998836092203 1101 38-39 0.22107178270812966 1101 40-41 0.24137485598335218 1101 42-43 0.39988963964758995 1101 44-45 0.3568199222445614 1101 46-47 0.3846864806610881 1101 48-49 0.22276452187479379 1101 50-51 0.14898950890603402 1101 52-53 0.18653095852259582 1101 54-55 0.3896549449568525 1101 56-57 0.2945129251819125 1101 58-59 0.48234047316632456 1101 60-61 0.33276543992548824 1101 62-63 0.2894267571964022 1101 64-65 0.40929674047067977 1101 66-67 0.3052770978301069 1101 68-69 0.4488398549408643 1101 70-71 0.3747475614461493 1101 72-73 0.06446291747520405 1101 74-75 0.370171554712563 1101 76-77 0.4161954175959224 1101 78-79 0.3855403209229209 1101 80-81 0.4195158508286454 1101 82-83 0.306342694064746 1101 84-85 0.4665601731132014 1101 86-87 0.457712213305399 1101 88-89 0.4012594371704523 1101 90-91 0.31476936904399366 1101 92-93 0.39920583854334524 1101 94-95 0.3913031581463571 1101 96-97 0.25738324616812847 1101 98-99 0.31150070448306977 1101 100-101 0.31701326488835235 2304 1 0.23964666744099006 2304 2 0.24230709974084874 2304 3 0.20500604963193325 2304 4 0.2915520560037379 2304 5 0.31131025205245066 2304 6 0.30577024860827606 2304 7 0.32121575211016307 2304 8 0.2661631451961384 2304 9 0.3577438821454919 2304 10-11 0.39646937777154534 2304 12-13 0.3849414117078638 2304 14-15 0.5473128287016849 2304 16-17 0.5082976637437966 2304 18-19 0.4862277185742201 2304 20-21 0.5451450710421781 2304 22-23 0.46644993647687016 2304 24-25 0.2765172755477394 2304 26-27 0.3258202325452757 2304 28-29 0.3056379372231177 2304 30-31 0.38937972219850536 2304 32-33 0.2847504876745788 2304 34-35 0.16976628855852738 2304 36-37 0.291927090687345 2304 38-39 0.17469829417308347 2304 40-41 0.259622822215249 2304 42-43 0.25283458568161876 2304 44-45 0.22509627364369322 2304 46-47 0.13928857405210948 2304 48-49 0.23199482381421888 2304 50-51 0.20851077387603567 2304 52-53 0.32857943548662405 2304 54-55 0.32278216273636673 2304 56-57 0.3777420322524492 2304 58-59 0.2691811257077745 2304 60-61 0.37408715214770183 2304 62-63 0.48609930446081506 2304 64-65 0.3766962855044653 2304 66-67 0.5071080917810349 2304 68-69 0.4413233099842202 2304 70-71 0.4110802741762072 2304 72-73 0.463769098008882 2304 74-75 0.5923576063257556 2304 76-77 0.5422149131635763 2304 78-79 0.5060237044509179 2304 80-81 0.3697503046763302 2304 82-83 0.4520804392258029 2304 84-85 0.5585230928038669 2304 86-87 0.4849261157295288 2304 88-89 0.3836030796965417 2304 90-91 0.47374307951769623 2304 92-93 0.38016216355411103 2304 94-95 0.3838883618606559 2304 96-97 0.3120513104644509 2304 98-99 0.30188630884405754 2304 100-101 0.4653750811533506 2202 1 0.12154092839901764 2202 2 0.09177472846879908 2202 3 0.07304711044262291 2202 4 0.16941562074628536 2202 5 0.03952154450622203 2202 6 0.10671550086068748 2202 7 0.0727148619623037 2202 8 0.16757506733085847 2202 9 0.03441868292192396 2202 10-11 0.15757210872764205 2202 12-13 0.17298034215647107 2202 14-15 0.22361691724290012 2202 16-17 0.12597934014508638 2202 18-19 0.19763765285821222 2202 20-21 0.23631693096886153 2202 22-23 0.18068524976799694 2202 24-25 0.13201002866252765 2202 26-27 0.17064728953227615 2202 28-29 0.0635817577845117 2202 30-31 0.11881880480539309 2202 32-33 0.018637477630086607 2202 34-35 0.08658916959735308 2202 36-37 0.13460286565679525 2202 38-39 -0.02402045564421229 2202 40-41 0.08436920681174342 2202 42-43 0.039580408271781664 2202 44-45 0.05558486104188631 2202 46-47 -0.044719714993078696 2202 48-49 0.18491126392437707 2202 50-51 0.16959969219291793 2202 52-53 0.2062335268049722 2202 54-55 0.3122557805954571 2202 56-57 0.37492149497732186 2202 58-59 0.2943214080410428 2202 60-61 0.35228318857799223 2202 62-63 0.24901567514235978 2202 64-65 0.2130144095872737 2202 66-67 0.31106088178799496 2202 68-69 0.35613495381983995 2202 70-71 0.3272095085905775 2202 72-73 0.26430125003268756 2202 74-75 0.2791285145689031 2202 76-77 0.16653725339007508 2202 78-79 0.18936721455680328 2202 80-81 0.12429486395813427 2202 82-83 0.23922976993400624 2202 84-85 0.2692387409893726 2202 86-87 0.2852029349052714 2202 88-89 0.25984466421819263 2202 90-91 0.20485255966550397 2202 92-93 0.19890626212227147 2202 94-95 0.3750112746173926 2202 96-97 0.37967605540566396 2202 98-99 0.28257126958400747 2202 100-101 0.4868573892819086 1102 1 0.06315497849230312 1102 2 0.08776266769107366 1102 3 0.1340321408831855 1102 4 0.05718583753208861 1102 5 0.2232822604429785 1102 6 -0.0181287074010541 1102 7 0.12002987323351988 1102 8 0.2091628656064728 1102 9 0.10092462622301923 1102 10-11 0.13747993121716462 1102 12-13 0.1275373414783374 1102 14-15 0.10353944677368077 1102 16-17 0.09711865397376584 1102 18-19 0.11958542730138078 1102 20-21 0.16769954845076995 1102 22-23 0.11999912864722972 1102 24-25 0.17392986474678196 1102 26-27 0.07897448656597561 1102 28-29 0.013928427342392524 1102 30-31 -0.015509939228337544 1102 32-33 0.08887690123453496 1102 34-35 0.09790305048438341 1102 36-37 0.2160989482129878 1102 38-39 0.043848022247296115 1102 40-41 -0.02061020584022799 1102 42-43 0.008930030782700271 1102 44-45 0.06118257176292019 1102 46-47 0.0993038805221289 1102 48-49 0.10135619559184761 1102 50-51 0.04872563215104009 1102 52-53 0.33311363129022453 1102 54-55 0.30458302180046104 1102 56-57 0.30793092204018535 1102 58-59 0.2517512998765241 1102 60-61 0.31418994392861777 1102 62-63 0.2660073272651218 1102 64-65 0.1941888948282866 1102 66-67 0.3662461679202593 1102 68-69 0.25713598632440693 1102 70-71 0.27365529858708015 1102 72-73 0.3432700304475276 1102 74-75 0.46513995995985624 1102 76-77 0.34257777655918886 1102 78-79 0.22094167610592308 1102 80-81 0.32279298893585917 1102 82-83 0.19303058640067405 1102 84-85 0.38430803725493234 1102 86-87 0.26466578155600473 1102 88-89 0.2744317401923624 1102 90-91 0.25016279541485886 1102 92-93 0.3322376378630807 1102 94-95 0.3189982782108274 1102 96-97 0.33318498980773725 1102 98-99 0.40156428399038546 1102 100-101 0.3179096253796132 2305 1 0.19264980742194737 2305 2 0.17351733398728442 2305 3 0.20707070406891148 2305 4 0.23252450755556708 2305 5 0.18755327584585757 2305 6 0.2458498626740706 2305 7 0.20394169451724764 2305 8 0.2927699086765898 2305 9 -0.0237736079061861 2305 10-11 0.04949873012639472 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58-59 -0.41046491351097103 1213 60-61 -0.41168170825226724 1213 62-63 -0.3650122335517878 1213 64-65 -0.1697667815720223 1213 66-67 -0.0983267965545025 1213 68-69 -0.09496812647986275 1213 70-71 -0.2762210545141741 1213 72-73 -0.2565858354593331 1213 74-75 -0.21199443826221653 1213 76-77 -0.29019744774218736 1213 78-79 -0.20508802809907678 1213 80-81 -0.13656559486456388 1213 82-83 -0.1335182009578375 1213 84-85 -0.12401377203155306 1213 86-87 -0.19110420292271613 1213 88-89 -0.241698696429836 1213 90-91 -0.17470086453334233 1213 92-93 -0.1639834768629811 1213 94-95 -0.18516000986139147 1213 96-97 -0.15691268066464303 1213 98-99 -0.2002769469337018 1213 100-101 -0.0744418591625795 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1262.0 3 284.0 4 271.0 5 294.0 6 843.0 7 2359.0 8 7216.0 9 11191.0 10 12625.0 11 9782.0 12 7259.0 13 5638.0 14 5439.0 15 5853.0 16 6299.0 17 6639.0 18 7048.0 19 7760.0 20 8744.0 21 9801.0 22 11299.0 23 12610.0 24 14263.0 25 16410.0 26 19530.0 27 23226.0 28 28758.0 29 35375.0 30 44297.0 31 56881.0 32 78803.0 33 114357.0 34 174178.0 35 297039.0 36 561823.0 37 879988.0 38 208938.0 39 120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.59571372150357 23.254814097355116 28.155289932784477 12.994182248356836 2 32.686155079395974 20.223281521610094 35.57034141999129 11.520221979002649 3 40.33010918068723 17.00385216668564 27.659070226702653 15.00696842592448 4 31.681859693436277 16.047593450599567 37.32644888651637 14.944097969447787 5 30.902510905740034 15.15843921617063 38.844597947793815 15.094451930295524 6 23.08703665764005 16.05895325183225 45.899333433229735 14.954676657297972 7 33.92068274025354 15.961729673070455 33.57801166025792 16.539575926418085 8 30.61170328426579 26.964290825101205 26.63221791179141 15.791787978841596 9 30.573483681854125 27.627767009340793 24.262911223816346 17.535838084988733 10-11 25.133833791954963 27.43639179340997 29.753328939086675 17.676445475548395 12-13 26.001324141765302 24.555359847510005 29.42320674580176 20.020109264922933 14-15 24.13764332813956 25.644264690989132 30.842924158845815 19.375167822025496 16-17 22.44604717579762 27.598222522824678 31.996818899305712 17.95891140207199 18-19 21.530751661029267 28.14560726917908 31.76369891645267 18.559942153338984 20-21 21.612199650439496 28.20509580956589 31.459200507154435 18.723504032840182 22-23 21.683873641640197 27.349816097551006 32.158841907084195 18.807468353724605 24-25 22.16842690694771 27.289914280435294 31.69089446583442 18.85076434678258 26-27 21.703821173335644 27.836727846826488 31.890318713488902 18.569132266348966 28-29 20.960448615924676 28.421681863481535 31.23884410001636 19.379025420577427 30-31 20.760065701004592 28.750015122398366 30.637923252665033 19.85199592393201 32-33 20.016272181086663 28.906375380971976 31.918925812631606 19.158426625309758 34-35 20.286043477953246 29.190190816057875 32.05531770216369 18.468448003825184 36-37 20.229043874383333 30.133221676058113 31.562697930703727 18.075036518854827 38-39 20.749154785502267 29.862687787688525 30.42433324036191 18.963824186447297 40-41 21.13689362110969 29.028654249055798 29.89983339080618 19.934618739028334 42-43 20.88752172454883 29.282205823119483 30.948178210480037 18.88209424185165 44-45 20.168319276007047 29.402066838425334 31.890569197903893 18.539044687663722 46-47 20.445633598552853 29.13841799755941 31.634518103015317 18.78143030087242 48-49 20.810680765073965 30.290999379544946 29.755270329958446 19.143049525422644 50-51 20.445998351947633 29.79637707810558 29.928575086476194 19.82904948347059 52-53 20.35127361869249 29.45827985742903 30.178805797796564 20.01164072608191 54-55 21.46189333145169 29.28109720008512 29.824960870003142 19.432048598460046 56-57 21.837658104759374 29.354871323751887 28.97505309802565 19.83241747346309 58-59 21.135392317581424 29.272764678338405 30.15286454624243 19.43897845783774 60-61 20.195681478814855 29.625438033826097 30.33189496792995 19.8469855194291 62-63 20.841388159066515 29.614021622263458 29.755728239687045 19.78886197898298 64-65 20.740792720505556 29.675524866536957 29.579777312483053 20.003905100474437 66-67 21.126467419603397 29.26647962083473 29.46081690166541 20.146236057896463 68-69 21.98007380417231 29.2312173965648 29.331527783594623 19.45718101566826 70-71 21.1580389001824 30.582244661604964 29.204988153442645 19.054728284769986 72-73 20.253226746544726 31.178031399878364 28.999312183215338 19.569429670361576 74-75 21.207769121395266 30.175005642972263 28.74650093851049 19.87072429712198 76-77 21.50525408662037 29.32695698420694 29.166326281672784 20.001462647499906 78-79 21.454721746105303 29.28208392188829 29.720322407147897 19.542871924858517 80-81 19.819334727886286 30.056344828189502 30.022470554974127 20.10184988895008 82-83 19.103767130619488 29.804201545351056 30.281973976072287 20.81005734795717 84-85 19.066838344395777 29.02701951068869 30.762417766969634 21.1437243779459 86-87 19.120383754292412 29.159514584848527 31.911554382472456 19.808547278386605 88-89 19.522003502420922 29.7785082710115 31.214474786720736 19.485013439846842 90-91 19.895098008920968 29.57171752671507 30.296504396263487 20.23668006810048 92-93 19.06716563052938 30.617358902408544 30.381606108020453 19.933869359041623 94-95 18.805608823564445 30.873058529961767 30.756071560441654 19.565261086032134 96-97 19.430120096196084 31.63799521997781 29.789906112025257 19.14197857180085 98-99 20.113449554096565 30.598653773160088 28.986692425384447 20.301204247358896 100-101 19.589836438311742 30.52772998238784 28.652512529652224 21.229921049648194 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1587.0 1 1118.5 2 549.5 3 582.5 4 654.0 5 625.0 6 767.0 7 1031.0 8 1286.0 9 1433.0 10 1546.0 11 1542.0 12 1369.0 13 1213.0 14 1119.0 15 994.0 16 906.5 17 916.5 18 992.5 19 1092.5 20 1192.5 21 1352.5 22 1617.0 23 2020.5 24 2590.5 25 3387.0 26 4772.0 27 7070.0 28 9418.0 29 11453.0 30 13985.5 31 17421.5 32 24784.5 33 36524.5 34 53101.0 35 80313.5 36 127346.5 37 188252.0 38 238395.5 39 245748.5 40 221682.5 41 192522.0 42 166012.5 43 155154.0 44 139458.0 45 119493.0 46 115104.0 47 103299.0 48 77934.5 49 59670.0 50 48621.5 51 41218.0 52 35380.5 53 29337.0 54 23813.5 55 17724.0 56 12601.0 57 9617.5 58 7825.5 59 6233.5 60 4877.0 61 3798.0 62 2937.0 63 2244.5 64 1686.0 65 1222.0 66 877.0 67 654.5 68 487.0 69 365.0 70 287.0 71 223.0 72 163.5 73 137.5 74 112.5 75 81.0 76 68.5 77 55.0 78 42.5 79 31.0 80 25.5 81 23.0 82 19.5 83 13.0 84 9.5 85 12.5 86 12.5 87 8.0 88 7.0 89 9.5 90 6.5 91 3.5 92 5.5 93 6.5 94 5.0 95 2.5 96 0.5 97 0.0 98 1.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29946164448940843 2 0.2746704214730588 3 0.2473184284146013 4 0.23444035298544963 5 0.24004435699064242 6 0.1873815643855525 7 0.15464824297773763 8 0.15323796382411295 9 0.13026525866375308 10-11 0.11519753928555258 12-13 0.13740943595514124 14-15 0.14026710687169652 16-17 0.13395796328969137 18-19 0.12057886763490991 20-21 0.12649832881920295 22-23 0.15650387344303326 24-25 0.5721650976692539 26-27 0.22347357693555248 28-29 0.19825555891218488 30-31 0.30022245298017963 32-33 0.3316753893669405 34-35 0.3392463616653467 36-37 0.3304135606505395 38-39 0.33731650598143925 40-41 0.3405453029910536 42-43 0.3512151781665035 44-45 0.3321949658972233 46-47 0.35232855644568084 48-49 0.34796782485223615 50-51 0.35310792124110507 52-53 0.33781752620706906 54-55 0.33039500434588653 56-57 0.3391535801420819 58-59 0.332324860029794 60-61 0.30992740031367577 62-63 0.29562048942624647 64-65 0.30697694787385577 66-67 0.3231580455312336 68-69 0.30950060530665774 70-71 0.29157521501190203 72-73 0.28725159602776323 74-75 0.27951361698748045 76-77 0.28181459876444703 78-79 0.2908515191304367 80-81 0.3005193538546269 82-83 0.3230467077033159 84-85 0.3140283436419791 86-87 0.31592108671658065 88-89 0.32085706375426704 90-91 0.31438091343038527 92-93 0.2969565433612593 94-95 0.2923731361119791 96-97 0.3088511346438043 98-99 0.31733136587020533 100-101 0.31818495588424134 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2694502.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.25896999580586 #Duplication Level Percentage of deduplicated Percentage of total 1 87.141854617501 36.82525019659851 2 5.672027291722496 4.793880622725859 3 2.0999834146033116 2.6622940832823385 4 1.0704565355859876 1.8094556247657017 5 0.7240212100650721 1.5298195296233468 6 0.4429819189136839 1.1231975772034724 7 0.35194531363508835 1.0410992514348778 8 0.3053923853386587 1.032445411917917 9 0.1970251024972116 0.7493470105365216 >10 1.621748179323278 13.84481431506697 >50 0.1672488732026577 4.960454041188858 >100 0.1723817364468276 16.317659790601443 >500 0.025834879279996174 7.252999196232049 >1k 0.006652853479171642 4.979733491111634 >5k 4.456884058138689E-4 1.0775498577103513 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6159 0.22857656071511545 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5912 0.21940974621655504 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5841 0.21677475095583523 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5395 0.20022252720539824 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 5299 0.19665971671203064 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 4951 0.18374452867357308 No Hit TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA 4298 0.1595099947968122 No Hit GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT 4141 0.1536833151357839 No Hit TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG 4135 0.15346063947994842 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 4019 0.14915557680046257 No Hit TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG 3509 0.13022814605444716 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 3016 0.11193162966663228 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 3007 0.11159761618287907 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 2955 0.1096677604989716 No Hit TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA 2949 0.10944508484313613 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2901 0.10766367959645234 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 3.7112609305912556E-4 0.0 0.0 2 4.453513116709507E-4 0.0 0.0 3 4.8246392097686327E-4 0.0 0.0 4 5.195765302827758E-4 0.0 0.0 5 5.93801748894601E-4 0.0 0.0 6 6.68026967506426E-4 0.0 0.0 7 7.051395768123386E-4 0.0 0.0 8 8.535900140359888E-4 0.0 0.0 9 9.278152326478139E-4 0.0 0.0 10-11 0.0017071800280719779 0.0 0.0 12-13 0.002523657432802054 0.0 0.0 14-15 0.0036927046259382992 0.0 0.0 16-17 0.004861751819074545 0.0 0.0 18-19 0.006253474668046266 0.0 0.0 20-21 0.007218402509999992 0.0 0.0 22-23 0.00842456231244215 0.0 0.0 24-25 0.01016885494982004 0.0 0.0 26-27 0.011709028236015412 0.0 0.0 28-29 0.013490433482699215 0.0 0.0 30-31 0.02031915359498713 0.0 0.0 32-33 0.022044889927712058 0.0 0.0 34-35 0.02378918256508995 0.0 0.0 36-37 0.026294283693239048 0.0 0.0 38-39 0.02866949068881745 0.0 0.0 40-41 0.031026141379742897 0.0 0.0 42-43 0.033178672719485824 0.0 0.0 44-45 0.03607345624534701 0.0 0.0 46-47 0.03941359108287914 0.0 0.0 48-49 0.04282795113902309 0.0 0.0 50-51 0.04624231119516704 0.0 0.0 52-53 0.050417479742082216 0.0 0.0 54-55 0.05446275415642668 0.0 0.0 56-57 0.05976985728717217 3.711260930591256E-5 0.0 58-59 0.06609755717383027 3.711260930591256E-5 0.0 60-61 0.07294483359077114 3.711260930591256E-5 0.0 62-63 0.08033024284264773 3.711260930591256E-5 0.0 64-65 0.0892558253807197 3.711260930591256E-5 3.711260930591256E-5 66-67 0.09949890554915156 3.711260930591256E-5 3.711260930591256E-5 68-69 0.11105948334794333 3.711260930591256E-5 3.711260930591256E-5 70-71 0.12354787637938291 3.711260930591256E-5 3.711260930591256E-5 72-73 0.13787334357146513 3.711260930591256E-5 7.422521861182512E-5 74-75 0.15488947493822605 3.711260930591256E-5 7.422521861182512E-5 76-77 0.17407669394938285 3.711260930591256E-5 7.422521861182512E-5 78-79 0.19627003431431855 3.711260930591256E-5 7.422521861182512E-5 80-81 0.2216736153842157 3.711260930591256E-5 7.422521861182512E-5 82-83 0.25013898672185064 3.711260930591256E-5 7.422521861182512E-5 84-85 0.28383723597161925 3.711260930591256E-5 7.422521861182512E-5 86-87 0.3238075161940871 3.711260930591256E-5 7.422521861182512E-5 88-89 0.37164566958940837 3.711260930591256E-5 7.422521861182512E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 355 0.0 49.421726 9 CGGAAAG 235 0.0 48.47117 5 TTGTGTT 37445 0.0 48.289562 1 AGAGCGG 360 0.0 46.105255 8 GAACCGG 95 3.8562575E-10 44.98514 1 AACCGGA 75 1.3696626E-7 44.3088 2 AACCGAG 105 1.031367E-9 40.70084 1 TCGGGAA 235 0.0 40.396397 3 CGAGGCG 130 5.456968E-12 40.179035 1 CGGCGAG 50 0.0016188256 37.98745 1 TGTGTTT 64005 0.0 37.969646 1 AAGCGGC 240 0.0 37.53936 9 TGACGTC 160 1.8189894E-12 35.56559 7 CGACGGG 95 8.7282024E-7 34.98844 1 CGGGAGA 435 0.0 34.9141 4 CCCGTGT 215 0.0 34.249447 38-39 TGAGACG 300 0.0 33.209362 6 TTGAGGC 3275 0.0 32.593697 6 GGACCGT 60 0.003958279 31.65621 1 TCGGAGA 180 5.456968E-12 31.640902 4 >>END_MODULE