FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_34311-1.34200000035761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_34311-1.34200000035761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694502
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG61590.22857656071511545No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG59120.21940974621655504No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC58410.21677475095583523No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC53950.20022252720539824No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT52990.19665971671203064No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT49510.18374452867357308No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA42980.1595099947968122No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT41410.1536833151357839No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAG41350.15346063947994842No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA40190.14915557680046257No Hit
TTGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGG35090.13022814605444716No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA30160.11193162966663228No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT30070.11159761618287907No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA29550.1096677604989716No Hit
TGTGTTTGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAA29490.10944508484313613No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29010.10766367959645234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC3550.049.4217269
CGGAAAG2350.048.471175
TTGTGTT374450.048.2895621
AGAGCGG3600.046.1052558
GAACCGG953.8562575E-1044.985141
AACCGGA751.3696626E-744.30882
AACCGAG1051.031367E-940.700841
TCGGGAA2350.040.3963973
CGAGGCG1305.456968E-1240.1790351
CGGCGAG500.001618825637.987451
TGTGTTT640050.037.9696461
AAGCGGC2400.037.539369
TGACGTC1601.8189894E-1235.565597
CGACGGG958.7282024E-734.988441
CGGGAGA4350.034.91414
CCCGTGT2150.034.24944738-39
TGAGACG3000.033.2093626
TTGAGGC32750.032.5936976
GGACCGT600.00395827931.656211
TCGGAGA1805.456968E-1231.6409024
Produced by FastQC (version 0.11.2)