FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_34310-1.34200000035810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_34310-1.34200000035810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5815882
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA283320.48714881079086547No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC246130.42320322179851655No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT144110.24778700805827905No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC114240.19642764416471997No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT108250.1861282605114753No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC87780.1509315354059797No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC76550.1316223403432188No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT74870.12873369851726701No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG74590.12825225821294173No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT71200.12242339167128906No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA67450.11597553045264673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC9200.055.137959
CGGGAGA12700.053.8029754
TTGTGTT715750.050.0120541
AGAGCGG10900.047.41168
CGGAAAG5850.045.4230165
CGGACGG1600.044.5077861
GAACCGG3650.037.7198371
AACCGGA3450.037.148882
GATCCGG5000.036.080981
AAGCGGC3400.033.4647069
TGTGTTT1074500.033.45072
TCGGGAG20950.032.8452573
ATTCGGA2350.032.3186452
CACCGAG2500.032.282981
CGGAGAG7750.031.8379765
CGAGTGG2250.031.649981
ATGCCGG2600.031.0413271
GTACCCC2750.029.3481641
ACCGGAA2750.029.3362733
TCGGGAA4600.028.88613