##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_34302-1.342000000358e7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8416479 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.966928688350556 33.0 33.0 33.0 27.0 33.0 2 30.59721791024489 33.0 33.0 33.0 27.0 33.0 3 30.435532245728886 33.0 33.0 33.0 22.0 33.0 4 33.91016659104122 37.0 37.0 37.0 27.0 37.0 5 33.979909532240264 37.0 37.0 37.0 27.0 37.0 6 34.35135939862738 37.0 37.0 37.0 27.0 37.0 7 33.981389842474506 37.0 37.0 37.0 27.0 37.0 8 34.097236029460774 37.0 37.0 37.0 27.0 37.0 9 33.68999007779857 37.0 37.0 37.0 22.0 37.0 10-11 33.713165446025585 37.0 37.0 37.0 22.0 37.0 12-13 33.67649506402856 37.0 37.0 37.0 22.0 37.0 14-15 35.2153491382798 40.0 37.0 40.0 22.0 40.0 16-17 35.14744223801901 40.0 37.0 40.0 22.0 40.0 18-19 35.18957624678919 40.0 37.0 40.0 22.0 40.0 20-21 35.20715895566305 40.0 37.0 40.0 22.0 40.0 22-23 35.7023988297244 40.0 37.0 40.0 27.0 40.0 24-25 35.153390449854385 38.5 37.0 40.0 22.0 40.0 26-27 34.971440194884345 37.0 37.0 40.0 18.5 40.0 28-29 34.80988938486034 37.0 37.0 40.0 15.0 40.0 30-31 34.85412284638267 37.0 37.0 40.0 18.5 40.0 32-33 34.65868173615119 37.0 37.0 40.0 15.0 40.0 34-35 34.66119852494137 37.0 37.0 40.0 15.0 40.0 36-37 34.5260124215839 37.0 37.0 40.0 15.0 40.0 38-39 34.624618976652826 37.0 37.0 40.0 18.5 40.0 40-41 34.35593815418538 37.0 37.0 40.0 15.0 40.0 42-43 34.19888839501649 37.0 35.0 40.0 15.0 40.0 44-45 34.097652771426155 37.0 33.0 40.0 15.0 40.0 46-47 34.01720677969968 37.0 33.0 40.0 15.0 40.0 48-49 33.917766503070936 37.0 33.0 40.0 15.0 40.0 50-51 32.9048476803661 37.0 33.0 38.5 15.0 38.5 52-53 33.34533835348488 37.0 33.0 38.5 15.0 40.0 54-55 34.03203750642044 37.0 33.0 40.0 15.0 40.0 56-57 34.25170418651315 37.0 33.0 40.0 18.5 40.0 58-59 33.83449563647696 37.0 33.0 40.0 15.0 40.0 60-61 33.60933164569174 37.0 33.0 40.0 15.0 40.0 62-63 33.30124467725756 37.0 33.0 40.0 15.0 40.0 64-65 33.09878733137693 37.0 33.0 38.5 15.0 40.0 66-67 32.891444094377235 37.0 33.0 37.0 15.0 40.0 68-69 32.87970492173746 37.0 33.0 37.0 15.0 40.0 70-71 32.53327781130328 37.0 33.0 37.0 15.0 40.0 72-73 32.61846301761105 37.0 33.0 37.0 18.5 37.0 74-75 32.605920421116714 37.0 33.0 37.0 22.0 37.0 76-77 32.31963585960352 37.0 33.0 37.0 22.0 37.0 78-79 31.941462873013762 37.0 33.0 37.0 22.0 37.0 80-81 31.50596823208375 35.0 33.0 37.0 15.0 37.0 82-83 31.22028689194139 33.0 33.0 37.0 15.0 37.0 84-85 30.958248752239506 33.0 33.0 37.0 15.0 37.0 86-87 30.818696808962514 33.0 33.0 37.0 15.0 37.0 88-89 30.712894430081747 33.0 33.0 37.0 15.0 37.0 90-91 30.569641592404615 33.0 27.0 37.0 15.0 37.0 92-93 30.382032736017045 33.0 27.0 37.0 15.0 37.0 94-95 30.23094419887461 33.0 27.0 37.0 15.0 37.0 96-97 30.093455054067146 33.0 27.0 37.0 15.0 37.0 98-99 29.950541788317892 33.0 27.0 37.0 15.0 37.0 100-101 28.713417808088156 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.12969272584498626 2306 2 0.2744416402727907 2306 3 0.26306935024265954 2306 4 0.41983765409341345 2306 5 0.331097449391784 2306 6 0.08159869943519027 2306 7 0.4561670663058237 2306 8 0.24704662656245802 2306 9 0.4851225133988706 2306 10-11 0.42251699280485155 2306 12-13 0.5005425985323839 2306 14-15 0.742150720173548 2306 16-17 0.6514210198668593 2306 18-19 0.5688131765506554 2306 20-21 0.6465542149365149 2306 22-23 0.4737235836298055 2306 24-25 0.6494430521701133 2306 26-27 0.45345171061713074 2306 28-29 0.46909644261059924 2306 30-31 0.3193834595933467 2306 32-33 0.5061656019453764 2306 34-35 0.4031949187265056 2306 36-37 0.40806437151429975 2306 38-39 0.2828948509045972 2306 40-41 0.424972987553609 2306 42-43 0.4253600294494717 2306 44-45 0.5968622915374908 2306 46-47 0.5738560163977766 2306 48-49 0.5331986121471672 2306 50-51 0.4955773007463762 2306 52-53 0.4466278169433693 2306 54-55 0.5194618981980526 2306 56-57 0.50528767280219 2306 58-59 0.568482428988716 2306 60-61 0.4992249339050332 2306 62-63 0.5203376047511341 2306 64-65 0.46590505827455075 2306 66-67 0.5635791456005066 2306 68-69 0.35306126583325437 2306 70-71 0.5241647012909709 2306 72-73 0.23795435101198592 2306 74-75 0.23479918197325844 2306 76-77 0.3349466320532528 2306 78-79 0.47288058088290086 2306 80-81 0.5389641109574939 2306 82-83 0.574503239671742 2306 84-85 0.6416220097193595 2306 86-87 0.5915406073799616 2306 88-89 0.6262437845262134 2306 90-91 0.4544386099088982 2306 92-93 0.40294259176505776 2306 94-95 0.3541856621077848 2306 96-97 0.3068402844249576 2306 98-99 0.46122502141922084 2306 100-101 0.3387479892312193 2307 1 0.07296657418237729 2307 2 0.05018598293917975 2307 3 0.07031979227363294 2307 4 0.14227291126263708 2307 5 0.10848848171431769 2307 6 -0.03820453671208668 2307 7 0.28702533344497994 2307 8 0.046633972835522286 2307 9 0.20953828785619777 2307 10-11 0.1087131283066114 2307 12-13 0.06664229505554431 2307 14-15 0.3337104634351675 2307 16-17 0.3183028849144307 2307 18-19 0.3087854403499364 2307 20-21 0.2377686473927767 2307 22-23 0.24365737582056823 2307 24-25 0.35838816979924104 2307 26-27 0.14917342974890602 2307 28-29 0.19820285390323278 2307 30-31 0.1830756020060278 2307 32-33 0.1937717856976917 2307 34-35 0.09470167885904601 2307 36-37 0.19623737868787572 2307 38-39 0.03975126344004565 2307 40-41 0.16999354955435564 2307 42-43 0.08384640012661038 2307 44-45 0.20879638210951157 2307 46-47 0.15230703754318142 2307 48-49 0.1015669668722552 2307 50-51 -0.03335619233813247 2307 52-53 -0.003499298031911735 2307 54-55 0.10195244600575393 2307 56-57 -0.0615461571082605 2307 58-59 0.20687513632692145 2307 60-61 0.09776377970705852 2307 62-63 0.192455641520489 2307 64-65 0.1737430400448048 2307 66-67 0.103627703241429 2307 68-69 0.08690113959550416 2307 70-71 0.16384885783622138 2307 72-73 -0.09081563566493855 2307 74-75 0.059445634871480024 2307 76-77 0.23020245799661865 2307 78-79 0.20256503467015818 2307 80-81 0.3610388120169645 2307 82-83 0.3002977717030362 2307 84-85 0.24004138909047512 2307 86-87 0.31503161741452246 2307 88-89 0.15248538614487117 2307 90-91 0.15497579198422784 2307 92-93 0.20554435520764613 2307 94-95 0.13280866934350044 2307 96-97 0.1543662936185406 2307 98-99 0.11783542735444641 2307 100-101 0.08982143938860077 2201 1 0.20062220435355727 2201 2 0.1911343987508083 2201 3 0.16628600311746666 2201 4 0.2689757291741728 2201 5 0.23178044482455817 2201 6 0.07433091987154938 2201 7 0.38927170673612466 2201 8 0.1526354129848997 2201 9 0.39687307833466434 2201 10-11 0.36395557990839933 2201 12-13 0.3161668028481728 2201 14-15 0.6665721085629244 2201 16-17 0.49368597475157117 2201 18-19 0.561990830953178 2201 20-21 0.4951730066104787 2201 22-23 0.35757124392039685 2201 24-25 0.4536049281547747 2201 26-27 0.3117595489022662 2201 28-29 0.4306275478955328 2201 30-31 0.4261058970923486 2201 32-33 0.4502493914709902 2201 34-35 0.31872170145697254 2201 36-37 0.3463139557692969 2201 38-39 0.29106806936764684 2201 40-41 0.43331061426631834 2201 42-43 0.44441790950802584 2201 44-45 0.4721642536947428 2201 46-47 0.48132967925047154 2201 48-49 0.4526152960536649 2201 50-51 0.31037379456390823 2201 52-53 0.3553408574118677 2201 54-55 0.46476323375399886 2201 56-57 0.3660693927531824 2201 58-59 0.5360269176820438 2201 60-61 0.4662837568493856 2201 62-63 0.6021033075790001 2201 64-65 0.5725858218131137 2201 66-67 0.5533833973390756 2201 68-69 0.49454386421960095 2201 70-71 0.4405023359605238 2201 72-73 0.26890537267051684 2201 74-75 0.3827734694380496 2201 76-77 0.43366687346384225 2201 78-79 0.5424527713219618 2201 80-81 0.6503515952427392 2201 82-83 0.6236268929762154 2201 84-85 0.5980705826597337 2201 86-87 0.488081270821457 2201 88-89 0.48877302592088867 2201 90-91 0.5153981155502976 2201 92-93 0.5648218586216238 2201 94-95 0.5214856270976789 2201 96-97 0.5204445946463174 2201 98-99 0.5294574785716222 2201 100-101 0.40331930441764996 1101 1 -0.003252513286042813 1101 2 -0.0362046227059345 1101 3 -0.035395619746175555 1101 4 0.05476145493975082 1101 5 -0.0792479033648874 1101 6 -0.038464973517641 1101 7 0.18050109376459034 1101 8 -0.028447367401845725 1101 9 0.07077326360194292 1101 10-11 0.05741051439373024 1101 12-13 0.05287043172020134 1101 14-15 0.19570609442327225 1101 16-17 0.0983696692883882 1101 18-19 0.22386431759856862 1101 20-21 0.17331756100646345 1101 22-23 0.14767829802939048 1101 24-25 0.11055730792973151 1101 26-27 -0.04308124310210104 1101 28-29 0.033032401933532185 1101 30-31 0.07835458088225522 1101 32-33 0.033299334159991645 1101 34-35 -0.06595864625914771 1101 36-37 -0.0786294396906797 1101 38-39 -0.10553093382727496 1101 40-41 -0.00500363895113054 1101 42-43 0.029271073269107717 1101 44-45 0.04041338961452823 1101 46-47 -0.055062889571296125 1101 48-49 -0.059859956017575655 1101 50-51 -0.1156136941549164 1101 52-53 -0.03954097950700941 1101 54-55 0.1256768274976494 1101 56-57 0.09313554677051172 1101 58-59 0.24461915129189293 1101 60-61 0.14464784148950827 1101 62-63 0.10145787453219413 1101 64-65 0.183185397234908 1101 66-67 0.11231225576413806 1101 68-69 0.03593162916152437 1101 70-71 0.12638714174779153 1101 72-73 -0.20109117454085634 1101 74-75 -0.10908448407381854 1101 76-77 -0.02435825506643141 1101 78-79 0.0619152401276466 1101 80-81 0.171362564690412 1101 82-83 0.13511584846791536 1101 84-85 0.14850319676512314 1101 86-87 0.11676365665424981 1101 88-89 0.13057053355499804 1101 90-91 -0.015894039819894346 1101 92-93 0.06910574496041377 1101 94-95 0.10115735608821197 1101 96-97 -0.028576217456635078 1101 98-99 0.03584637383821132 1101 100-101 0.03187598578442419 2304 1 0.022581048210884802 2304 2 -0.16088106457899443 2304 3 -0.07303382145456894 2304 4 0.013135420840072243 2304 5 -0.06081547194422399 2304 6 -0.018071948995327602 2304 7 0.1406132084534022 2304 8 -0.032365555539534796 2304 9 0.06518815099094866 2304 10-11 0.028482047752753203 2304 12-13 0.019059098691911913 2304 14-15 0.17263017594764563 2304 16-17 0.09044849637382413 2304 18-19 0.14123715363331257 2304 20-21 0.07895717358832854 2304 22-23 0.004351588935506356 2304 24-25 0.055329971907923436 2304 26-27 -0.05218848520505048 2304 28-29 0.03346473109585446 2304 30-31 0.15626113863216773 2304 32-33 0.08591852529647781 2304 34-35 0.05602529974779458 2304 36-37 0.0674477312471069 2304 38-39 0.02861323715126929 2304 40-41 0.05021491557447888 2304 42-43 0.16025199131082957 2304 44-45 -0.012358895310939033 2304 46-47 -0.028992014976402913 2304 48-49 0.014965815856697873 2304 50-51 0.03580423691890644 2304 52-53 0.01868901037006765 2304 54-55 0.2269158206467452 2304 56-57 0.1849515184545112 2304 58-59 0.09402359163772189 2304 60-61 0.08969993328444303 2304 62-63 0.08439633899267562 2304 64-65 0.11409423610759717 2304 66-67 0.20651009940951326 2304 68-69 0.10941457150524059 2304 70-71 0.11191135675900199 2304 72-73 0.10777293659630516 2304 74-75 0.3565317448118037 2304 76-77 0.22211275837940292 2304 78-79 0.22996628721826085 2304 80-81 0.12330458599110017 2304 82-83 0.14414134264028533 2304 84-85 0.21101373557342384 2304 86-87 0.13337297046104624 2304 88-89 0.211791047681114 2304 90-91 0.14128143315128838 2304 92-93 0.12960960553707324 2304 94-95 0.15983387542479832 2304 96-97 0.17996644027666164 2304 98-99 0.15699596629170642 2304 100-101 0.14331953330307456 2202 1 0.028273449717190147 2202 2 -0.09106587842553893 2202 3 -0.045621020511593 2202 4 -0.014486061973329356 2202 5 -0.08365169958667451 2202 6 0.08265827724172681 2202 7 0.12114491420111761 2202 8 0.05080329441420872 2202 9 0.005191395579281277 2202 10-11 -0.0013142282255955706 2202 12-13 -0.01751450083138195 2202 14-15 0.0380784250713333 2202 16-17 0.017580692433526224 2202 18-19 0.09108260508899235 2202 20-21 0.048913424585251164 2202 22-23 0.12244218952565689 2202 24-25 0.10109243348234997 2202 26-27 0.1189960678404205 2202 28-29 0.009510484973418443 2202 30-31 0.12988640650037553 2202 32-33 -0.03390281133336259 2202 34-35 0.0575793505611486 2202 36-37 0.08349327001178608 2202 38-39 0.03709024144369977 2202 40-41 0.02465357672155477 2202 42-43 0.005458462495560923 2202 44-45 0.09133135137563642 2202 46-47 0.04877935622341312 2202 48-49 0.14204626179503776 2202 50-51 0.045296307483162934 2202 52-53 0.04883838651193173 2202 54-55 0.2612870109094203 2202 56-57 0.34872993420997744 2202 58-59 0.18289195449872153 2202 60-61 0.20583244737195372 2202 62-63 0.24811166553059394 2202 64-65 0.2727114897158742 2202 66-67 0.327722381326474 2202 68-69 0.23798885901695854 2202 70-71 0.22437610289755838 2202 72-73 0.2830901829030381 2202 74-75 0.3195857241103681 2202 76-77 0.3161188521137106 2202 78-79 0.30764351390834577 2202 80-81 0.28226657011931167 2202 82-83 0.28453499288809425 2202 84-85 0.30424177866631297 2202 86-87 0.3159187834014503 2202 88-89 0.18130211379456895 2202 90-91 0.21495669332930234 2202 92-93 0.19662824025956738 2202 94-95 0.27114864372054015 2202 96-97 0.25957921694580577 2202 98-99 0.18436219361671036 2202 100-101 0.21886771093807766 1102 1 0.025620424215432536 1102 2 -0.08990978600943933 1102 3 -0.06714731945164232 1102 4 -0.0372691685650679 1102 5 -0.048128031057743215 1102 6 0.04917801081793982 1102 7 0.05640797063227154 1102 8 0.11717067376839907 1102 9 -5.855964804837299E-4 1102 10-11 0.01750658019206952 1102 12-13 0.008185327446604163 1102 14-15 -0.015112117642786416 1102 16-17 -0.04003717354875391 1102 18-19 0.033254667437581986 1102 20-21 0.0029516713567758757 1102 22-23 0.0878219874407904 1102 24-25 0.037842055254330376 1102 26-27 0.1856321537504826 1102 28-29 0.003934347869211763 1102 30-31 0.047159436718992254 1102 32-33 -0.0408451548401203 1102 34-35 0.013488268577042106 1102 36-37 0.03144329771863141 1102 38-39 0.05032354632343328 1102 40-41 -0.08900859394363891 1102 42-43 -0.014896408229496672 1102 44-45 0.005969865690836684 1102 46-47 0.08981885966717584 1102 48-49 0.04096102043131111 1102 50-51 0.0857229037058147 1102 52-53 0.07335512391991017 1102 54-55 0.18824618080056865 1102 56-57 0.31547119898716147 1102 58-59 0.2190405991500981 1102 60-61 0.22071767677511644 1102 62-63 0.2928383622128834 1102 64-65 0.19917431122831175 1102 66-67 0.3246529110207348 1102 68-69 0.2965691225513254 1102 70-71 0.20998134822827552 1102 72-73 0.26225629697596275 1102 74-75 0.4109344174029701 1102 76-77 0.27258086604230414 1102 78-79 0.26218923005181693 1102 80-81 0.25129859023486745 1102 82-83 0.2348327181270129 1102 84-85 0.2935218775033377 1102 86-87 0.21672962060454992 1102 88-89 0.1599564675186933 1102 90-91 0.11109909160484222 1102 92-93 0.1489415735839863 1102 94-95 0.1482547283646376 1102 96-97 0.13830357537579374 1102 98-99 0.1792312855615421 1102 100-101 0.16217059379575005 2305 1 0.1816283042813005 2305 2 0.01413662016355488 2305 3 0.00797421708967505 2305 4 -0.015252837477163439 2305 5 0.04943343567018843 2305 6 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0.019879989897827244 1213 92-93 0.005186777506100526 1213 94-95 0.115460033738497 1213 96-97 0.08231546051888117 1213 98-99 0.07380977719525461 1213 100-101 0.1313475995667659 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4110.0 3 1225.0 4 1306.0 5 1112.0 6 2934.0 7 15553.0 8 77264.0 9 178617.0 10 186282.0 11 114788.0 12 64586.0 13 40950.0 14 29739.0 15 23737.0 16 21870.0 17 21975.0 18 23424.0 19 25544.0 20 27930.0 21 30957.0 22 34869.0 23 39126.0 24 43670.0 25 51232.0 26 60749.0 27 73188.0 28 89102.0 29 110052.0 30 135874.0 31 175609.0 32 240497.0 33 340893.0 34 516145.0 35 871484.0 36 1661293.0 37 2482536.0 38 596014.0 39 243.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.82317963756731 23.727359873850236 25.579135809295728 13.870324679286728 2 30.227430293483003 24.362273847809746 33.47586770729071 11.934428151416542 3 36.8648793767497 20.523020755317468 27.604372128301442 15.007727739631394 4 26.817816202970512 18.520351667050544 36.96474588075513 17.697086249223812 5 33.30713511728932 17.18198823617126 33.29761895971128 16.213257686828136 6 27.259400516740502 17.630207356856275 40.21876657221065 14.891625554192581 7 34.32022856979661 18.644919534102215 31.14390805801351 15.890943838087669 8 27.06228374278865 29.94788288964733 27.411674718215618 15.578158649348401 9 32.61009224628227 26.75399159268968 23.88722821205343 16.748687948974617 10-11 26.536524369920627 29.281303059738086 27.374905403936197 16.807267166405094 12-13 28.631263081548912 24.248158945994795 27.15094810592973 19.96962986652656 14-15 26.042539809981015 24.79475567296022 28.407104610862994 20.75559990619578 16-17 26.061658466849778 26.24015959513015 29.542707080522945 18.155474857497126 18-19 25.622959678170037 26.61891479636856 29.729563027264366 18.028562498197036 20-21 24.813557793633983 28.172444051277797 28.92361389522643 18.090384259861796 22-23 27.077225776590264 26.635533064270117 28.236566508834837 18.050674650304785 24-25 24.60448042812045 28.74542659209802 28.433811086019624 18.216281893761906 26-27 22.372522253005585 30.76973961585451 28.414297296713155 18.443440834426756 28-29 23.07775963126919 29.65649169171724 28.097325181353145 19.168423495660424 30-31 24.97072445859837 27.701040727397917 28.21698024898257 19.111254565021138 32-33 23.262622801985717 27.04328246972001 29.632215115843824 20.06187961245045 34-35 20.685288530036832 27.274852592264214 32.92748779855517 19.112371079143784 36-37 21.21957774402519 27.955279534795203 30.2344375786277 20.590705142551908 38-39 20.730150315036706 27.553979575590542 27.853771561892167 23.86209854748059 40-41 21.855060102912983 27.022353607825973 28.558960726945585 22.563625562315455 42-43 23.73117326090645 27.191850997878724 30.116659935964734 18.96031580525009 44-45 22.068116704019992 28.762920007340924 30.560743324100354 18.60821996453873 46-47 23.331286690016167 29.293367927745916 28.871972832404712 18.503372549833202 48-49 21.40688291691993 29.00838390806626 29.931301743668786 19.653431431345023 50-51 20.275153829538908 28.03884246904913 30.542819105190244 21.14318459622172 52-53 23.639926462760297 27.589405792541864 28.34814453899206 20.422523205705776 54-55 25.288488927466997 27.477056036990476 27.90071254328787 19.33374249225466 56-57 25.743350055555293 27.092062031177726 27.251247013605084 19.9133408996619 58-59 24.414138995664466 26.79339904911375 27.996547260210324 20.79591469501146 60-61 21.515720860198112 27.101614428269976 27.938483929937654 23.44418078159426 62-63 23.161184957270255 27.22249795780233 28.08387935929511 21.532437725632306 64-65 21.654309733369477 28.225728072464403 29.669892573010447 20.450069621155677 66-67 20.734736379871023 28.273244827019816 28.709345705050314 22.282673088058846 68-69 20.303613805061058 27.292777401462036 32.02888531701786 20.374723476459053 70-71 19.851743408221466 30.725347213305977 30.15349661430199 19.269412764170568 72-73 19.78366196928007 33.868386510380496 27.309290902270938 19.038660618068487 74-75 20.563561867990504 33.48194823671488 26.826792121068934 19.127697774225684 76-77 20.74430185852062 32.58245767380857 27.335994944305387 19.33724552336543 78-79 20.67771855459094 32.38231203820799 27.803103630752997 19.136865776448072 80-81 19.929580292968957 32.87721561631961 27.571450822097525 19.621753268613908 82-83 19.242685624875065 32.324575325396225 28.0866199950629 20.346119054665802 84-85 19.360184585723808 31.563649846822166 28.354936588286783 20.721228979167243 86-87 19.306867234008216 31.442194170928207 29.022541120502442 20.228397474561138 88-89 19.58944965583152 31.63367181027174 28.65697709830492 20.11990143559182 90-91 20.14073781095983 30.885866226375818 28.21090982033051 20.762486142333838 92-93 19.786927229292512 31.626102117488415 27.883505426262918 20.70346522695615 94-95 19.485930225242228 32.19121423750529 27.770419607551545 20.552435929700934 96-97 19.994570135530896 32.465816345441404 27.270445507508196 20.26916801151951 98-99 20.43454557525283 31.326194702057954 27.119017699417768 21.120242023271448 100-101 20.493805073143207 31.38259353595496 26.744049044742795 21.379552346159034 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2337.0 1 1856.0 2 1042.5 3 870.0 4 1021.0 5 1081.0 6 1370.5 7 1869.0 8 2376.5 9 2811.0 10 3147.0 11 3327.5 12 3243.0 13 2942.5 14 2651.0 15 2448.5 16 2334.5 17 2362.5 18 2588.5 19 2881.5 20 3398.0 21 4298.0 22 5483.0 23 7186.0 24 9490.0 25 12478.5 26 16475.0 27 21755.0 28 27543.0 29 33668.0 30 41001.5 31 49501.0 32 61614.5 33 85968.0 34 126692.5 35 203918.0 36 306922.0 37 411671.5 38 532650.5 39 596165.0 40 589866.5 41 537240.5 42 480792.0 43 483759.0 44 473083.5 45 446398.0 46 449627.0 47 407667.5 48 321780.0 49 272217.0 50 234332.0 51 200753.0 52 179849.0 53 157153.0 54 126012.0 55 94284.0 56 73465.0 57 59198.5 58 48591.0 59 39040.5 60 30922.0 61 24659.5 62 19199.5 63 14839.0 64 11652.0 65 9144.0 66 6996.0 67 5422.0 68 4239.0 69 3071.5 70 2303.5 71 1772.5 72 1317.0 73 1008.0 74 807.0 75 617.0 76 437.5 77 329.5 78 260.0 79 211.5 80 175.5 81 142.0 82 110.5 83 94.0 84 82.5 85 55.0 86 41.5 87 36.5 88 32.5 89 24.0 90 19.5 91 18.0 92 14.0 93 16.0 94 13.0 95 6.5 96 3.5 97 1.5 98 1.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2992106319043866 2 0.27543584437150026 3 0.24655203203144688 4 0.23374382565440965 5 0.24039743935676663 6 0.18706159666055128 7 0.15349649182276817 8 0.15104891249654398 9 0.12994745189764034 10-11 0.11502434687949675 12-13 0.13676740594255626 14-15 0.13948825868869869 16-17 0.13319108857753936 18-19 0.1209116068607787 20-21 0.12662064504646184 22-23 0.1559500118755123 24-25 0.5702265757450354 26-27 0.22462481044626856 28-29 0.1976895564047626 30-31 0.29808783459211385 32-33 0.3299598323717079 34-35 0.3378550579167369 36-37 0.32861128745167667 38-39 0.33659562389450504 40-41 0.33999966019044303 42-43 0.3509543598932523 44-45 0.33137966600997876 46-47 0.35139991438224943 48-49 0.3471879392795966 50-51 0.3526355854984014 52-53 0.3368154307757436 54-55 0.32941922625839143 56-57 0.33789664300237665 58-59 0.33004894326950734 60-61 0.3080088478804498 62-63 0.2944699321414572 64-65 0.3054780983829461 66-67 0.3210487425917655 68-69 0.30794944061525015 70-71 0.28918862626521136 72-73 0.28528556894159657 74-75 0.2769566703606104 76-77 0.2800102037918707 78-79 0.2888143604944538 80-81 0.29796307933519467 82-83 0.3200625819894519 84-85 0.31166239469022616 86-87 0.3138188784169722 88-89 0.31887443668545956 90-91 0.31209606772618337 92-93 0.2945649837657766 94-95 0.2904658824670031 96-97 0.30708803526985573 98-99 0.3153575265856423 100-101 0.31688429330127243 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8416479.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.58783309745932 #Duplication Level Percentage of deduplicated Percentage of total 1 91.25770277872381 39.77725517576563 2 4.068472753915017 3.5467182271841686 3 1.2756698265015705 1.66811050455046 4 0.6890063690065322 1.2012917846137279 5 0.4294991461068175 0.9360468548002626 6 0.30928352421248206 0.8088599179900612 7 0.2896804966227327 0.8838581596866562 8 0.18639049703354235 0.6499486300520424 9 0.15218579044062444 0.5970103950177773 >10 1.0488794777762889 9.307952268367508 >50 0.11720929841423215 3.5798616243531334 >100 0.13227376003223865 12.69398083595791 >500 0.024427692959515322 7.314210698051893 >1k 0.01865862591188246 14.667856754169916 >5k 4.6770296550776913E-4 1.235392399815325 >10k+ 1.9225937719717136E-4 1.1316457696236453 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 17474 0.20761651041961848 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 16633 0.19762420841304304 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 13538 0.16085111125448062 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 13289 0.15789262944753976 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 11890 0.14127047664468717 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 11186 0.1329059337045812 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10517 0.12495724162087257 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 9782 0.116224373636529 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0019366768455074859 0.0 0.0 2 0.002150543000226104 0.0 0.0 3 0.002471342232304031 0.0 0.0 4 0.002602038215743187 0.0 0.0 5 0.00298224471302073 0.0 0.0 6 0.00335056975725835 0.0 1.1881453039923227E-5 7 0.003932760956214588 0.0 1.1881453039923227E-5 8 0.004598122326450289 0.0 1.1881453039923227E-5 9 0.005239720790606143 0.0 1.1881453039923227E-5 10-11 0.008008099348908254 0.0 1.782217955988484E-5 12-13 0.010734892821570634 0.0 2.3762906079846454E-5 14-15 0.01369931535503148 0.0 2.3762906079846454E-5 16-17 0.01623600557905509 0.0 4.752581215969291E-5 18-19 0.01922419101859578 0.0 4.752581215969291E-5 20-21 0.022853974922292325 0.0 4.752581215969291E-5 22-23 0.02732140126530346 0.0 5.9407265199616135E-5 24-25 0.03218091555863206 0.0 5.9407265199616135E-5 26-27 0.03730776254535893 0.0 5.9407265199616135E-5 28-29 0.04308214872276162 0.0 5.9407265199616135E-5 30-31 0.06689258061476777 5.9407265199616135E-6 5.9407265199616135E-5 32-33 0.07399174880612189 1.1881453039923227E-5 5.9407265199616135E-5 34-35 0.08043743708028024 1.1881453039923227E-5 5.9407265199616135E-5 36-37 0.08697223625223802 1.1881453039923227E-5 5.9407265199616135E-5 38-39 0.09404170081099233 1.1881453039923227E-5 5.9407265199616135E-5 40-41 0.1019250449029814 1.1881453039923227E-5 6.534799171957775E-5 42-43 0.11001037369664915 1.1881453039923227E-5 7.128871823953936E-5 44-45 0.11933137360646892 2.3762906079846454E-5 7.128871823953936E-5 46-47 0.1291335723644056 2.3762906079846454E-5 7.128871823953936E-5 48-49 0.1388644824041027 2.3762906079846454E-5 7.722944475950097E-5 50-51 0.14992017445775127 2.3762906079846454E-5 8.317017127946258E-5 52-53 0.16134419155563745 2.3762906079846454E-5 8.317017127946258E-5 54-55 0.17412269430007488 2.3762906079846454E-5 1.0693307735930904E-4 56-57 0.1880002314507052 2.3762906079846454E-5 1.0693307735930904E-4 58-59 0.20248372270637163 2.3762906079846454E-5 1.0693307735930904E-4 60-61 0.21829199597598947 2.3762906079846454E-5 1.0693307735930904E-4 62-63 0.2350507854888012 2.3762906079846454E-5 1.0693307735930904E-4 64-65 0.25283732068956627 2.3762906079846454E-5 1.1287380387927065E-4 66-67 0.27327341991823423 2.3762906079846454E-5 1.1881453039923227E-4 68-69 0.2960976912079267 2.3762906079846454E-5 1.1881453039923227E-4 70-71 0.3201516928872513 2.3762906079846454E-5 1.1881453039923227E-4 72-73 0.3461601935916433 2.3762906079846454E-5 1.1881453039923227E-4 74-75 0.37505588738473655 2.3762906079846454E-5 1.1881453039923227E-4 76-77 0.40644074558969373 2.3762906079846454E-5 1.1881453039923227E-4 78-79 0.4425306591984606 2.3762906079846454E-5 1.1881453039923227E-4 80-81 0.4838543528713135 2.3762906079846454E-5 1.247552569191939E-4 82-83 0.5300137979314152 2.3762906079846454E-5 1.306959834391555E-4 84-85 0.5821377324175585 2.3762906079846454E-5 1.4257743647907872E-4 86-87 0.6391330626500702 2.3762906079846454E-5 1.4257743647907872E-4 88-89 0.7045345209083276 2.3762906079846454E-5 1.4257743647907872E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4940 0.0 83.87509 9 CGGGAGA 7195 0.0 81.31751 4 AGAGCGG 5445 0.0 79.23421 8 CGGAAAG 3810 0.0 76.84436 5 AAGCGGC 2045 0.0 75.8059 9 GAGAGCG 6260 0.0 74.14736 7 ATTCGGA 1000 0.0 72.626625 2 TCGGGAG 7765 0.0 70.03676 3 TCGGGAA 2770 0.0 67.67063 3 GAACCGG 1175 0.0 67.47455 1 ACCGGAA 1555 0.0 66.52869 3 GGATCGG 3225 0.0 65.94922 1 GTTCGGA 1045 0.0 65.41098 2 GATCCGG 1745 0.0 64.750465 1 TCGGAGA 1740 0.0 64.35979 4 CGGAGAG 3595 0.0 63.22494 5 AACCGGA 1200 0.0 62.50004 2 ATCCGGA 1835 0.0 60.014523 2 AAGCGTC 885 0.0 57.85342 9 CCGGAAA 2495 0.0 57.816902 4 >>END_MODULE