FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_34302-1.342000000358e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_34302-1.342000000358e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416479
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC174740.20761651041961848No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG166330.19762420841304304No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA135380.16085111125448062No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA132890.15789262944753976No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC118900.14127047664468717No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG111860.1329059337045812No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC105170.12495724162087257No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA97820.116224373636529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC49400.083.875099
CGGGAGA71950.081.317514
AGAGCGG54450.079.234218
CGGAAAG38100.076.844365
AAGCGGC20450.075.80599
GAGAGCG62600.074.147367
ATTCGGA10000.072.6266252
TCGGGAG77650.070.036763
TCGGGAA27700.067.670633
GAACCGG11750.067.474551
ACCGGAA15550.066.528693
GGATCGG32250.065.949221
GTTCGGA10450.065.410982
GATCCGG17450.064.7504651
TCGGAGA17400.064.359794
CGGAGAG35950.063.224945
AACCGGA12000.062.500042
ATCCGGA18350.060.0145232
AAGCGTC8850.057.853429
CCGGAAA24950.057.8169024
Produced by FastQC (version 0.11.2)