FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_34298-1.342000000357ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_34298-1.342000000357ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5401618
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC149160.27613948265130933No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG139060.2574413814527425No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA99780.184722429464653No Hit
TGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCA97380.18027931630855792No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80870.1497144003889205No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80850.14967737444595305No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA70980.13140507159151205No Hit
GTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT63090.11679833709084946No Hit
TGTGTTTGAGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAG60400.11181834776172622No Hit
TTGTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTC59510.11017069329967429No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT58950.10913396689658543No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT58100.10756036432046842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC13500.070.943529
CGGGAGA17850.069.391834
AGAGCGG14700.068.702658
CGGAAAG10350.068.3206565
GAGAGCG15950.066.292367
ATTCGGA3550.065.524362
ACCGGAA4250.062.5353133
AAGCGGC5950.060.5608189
TCGGGAA7900.054.6689223
GAACCGG3600.054.075571
TCGGGAG22300.053.8445633
AACCGGA3300.053.225882
TCGGAGA6150.051.701814
GGTCCGG3050.051.3728751
GGATCGG9650.051.1712231
CGGAGAG11900.049.8504565
GATCCGG5450.047.916591
AAAGCGG8550.047.6917848
TTCGGAG3750.046.8270383
TCGGAAA9800.045.5205844
Produced by FastQC (version 0.11.2)