##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_34274-2.3420000003583a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4679685 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23770702515233 33.0 33.0 33.0 33.0 33.0 2 32.233564011252895 33.0 33.0 33.0 33.0 33.0 3 32.21171147203284 33.0 33.0 33.0 33.0 33.0 4 36.00839607794114 37.0 37.0 37.0 37.0 37.0 5 36.03024477074846 37.0 37.0 37.0 37.0 37.0 6 36.03577655333639 37.0 37.0 37.0 33.0 37.0 7 35.991536823525514 37.0 37.0 37.0 33.0 37.0 8 35.9265848021822 37.0 37.0 37.0 33.0 37.0 9 35.8806985940293 37.0 37.0 37.0 33.0 37.0 10-11 35.844433332585425 37.0 37.0 37.0 33.0 37.0 12-13 35.83992726006131 37.0 37.0 37.0 33.0 37.0 14-15 37.688063192287515 40.0 37.0 40.0 33.0 40.0 16-17 37.67427412315145 40.0 37.0 40.0 33.0 40.0 18-19 37.63261448153027 40.0 37.0 40.0 33.0 40.0 20-21 37.6215390565818 40.0 37.0 40.0 33.0 40.0 22-23 37.600989275987594 40.0 37.0 40.0 33.0 40.0 24-25 37.36009881006948 40.0 37.0 40.0 33.0 40.0 26-27 37.31934414389003 40.0 37.0 40.0 33.0 40.0 28-29 37.29944921506469 40.0 37.0 40.0 33.0 40.0 30-31 37.29096392171695 40.0 37.0 40.0 33.0 40.0 32-33 37.206465178746 40.0 37.0 40.0 33.0 40.0 34-35 37.133696391958004 40.0 37.0 40.0 33.0 40.0 36-37 37.058351042858654 40.0 37.0 40.0 33.0 40.0 38-39 37.0097741621498 40.0 37.0 40.0 33.0 40.0 40-41 36.91046993547643 40.0 37.0 40.0 33.0 40.0 42-43 36.809062041569035 40.0 37.0 40.0 33.0 40.0 44-45 36.70531072070022 40.0 37.0 40.0 33.0 40.0 46-47 36.60362844935076 40.0 37.0 40.0 33.0 40.0 48-49 36.4808315089584 38.5 37.0 40.0 33.0 40.0 50-51 35.32914138451626 37.0 35.0 38.5 30.0 38.5 52-53 35.74607350708435 37.0 37.0 38.5 33.0 40.0 54-55 36.49189731787503 37.0 37.0 40.0 33.0 40.0 56-57 36.54531042153478 37.0 37.0 40.0 33.0 40.0 58-59 36.3834936539532 37.0 37.0 40.0 33.0 40.0 60-61 36.140765243814485 37.0 37.0 40.0 33.0 40.0 62-63 35.87717517311529 37.0 37.0 40.0 33.0 40.0 64-65 35.63952007453493 37.0 37.0 40.0 30.0 40.0 66-67 35.35483990909645 37.0 37.0 40.0 27.0 40.0 68-69 35.13352939353824 37.0 33.0 37.0 27.0 40.0 70-71 34.83169038086966 37.0 33.0 37.0 27.0 40.0 72-73 34.584855284062925 37.0 33.0 37.0 27.0 40.0 74-75 34.330835195103944 37.0 33.0 37.0 27.0 38.5 76-77 34.082529593338016 37.0 33.0 37.0 27.0 37.0 78-79 33.82922942463007 37.0 33.0 37.0 27.0 37.0 80-81 33.58711035037615 37.0 33.0 37.0 27.0 37.0 82-83 33.37567603375014 37.0 33.0 37.0 27.0 37.0 84-85 33.1126074725115 37.0 33.0 37.0 27.0 37.0 86-87 32.9783658515477 37.0 33.0 37.0 27.0 37.0 88-89 32.857759977434384 37.0 33.0 37.0 27.0 37.0 90-91 32.71198755899168 37.0 33.0 37.0 27.0 37.0 92-93 32.55024227058018 33.0 33.0 37.0 27.0 37.0 94-95 32.4065734125267 33.0 33.0 37.0 22.0 37.0 96-97 32.263624688413856 33.0 33.0 37.0 22.0 37.0 98-99 32.10977704695936 33.0 33.0 37.0 22.0 37.0 100-101 30.809718709699478 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14729901774394705 2306 2 0.18974158686593512 2306 3 0.18928040070458962 2306 4 0.2992108201334176 2306 5 0.3013300787226143 2306 6 0.2373224573345638 2306 7 0.33269464865938403 2306 8 0.23799277631181326 2306 9 0.30979225280985645 2306 10-11 0.2607264046237461 2306 12-13 0.26527534117990825 2306 14-15 0.4290080795949436 2306 16-17 0.3466930107461721 2306 18-19 0.35044132819071905 2306 20-21 0.37142660838868125 2306 22-23 0.36820121441508746 2306 24-25 0.3949820742267036 2306 26-27 0.3253530710496122 2306 28-29 0.20996181246655965 2306 30-31 0.2210244288714236 2306 32-33 0.2734071471605759 2306 34-35 0.2531825101763374 2306 36-37 0.2978580871372145 2306 38-39 0.2705776173409973 2306 40-41 0.17484595644516077 2306 42-43 0.19434672147480825 2306 44-45 0.34613175708087596 2306 46-47 0.18974191719487976 2306 48-49 0.2340177978013216 2306 50-51 0.11449939513669705 2306 52-53 0.10787179091711607 2306 54-55 0.27346317646687623 2306 56-57 0.23996165495360344 2306 58-59 0.0992037072822427 2306 60-61 0.21474394666340402 2306 62-63 0.15647251108487836 2306 64-65 0.11509201869898789 2306 66-67 0.19360963362257877 2306 68-69 0.05507998604615949 2306 70-71 0.18600399711156257 2306 72-73 0.1843007623001256 2306 74-75 0.22267479691908676 2306 76-77 0.1604139418428474 2306 78-79 0.19432228136995633 2306 80-81 0.254601216124108 2306 82-83 0.14605195912640312 2306 84-85 0.2743952944537398 2306 86-87 0.2102440885711232 2306 88-89 0.16749004091919062 2306 90-91 -0.0013006303420937115 2306 92-93 0.11890510859474546 2306 94-95 0.11326460208779565 2306 96-97 0.1187698915110289 2306 98-99 0.10893630880351424 2306 100-101 0.13725483147602446 2307 1 0.20966039143750237 2307 2 0.24105567044139775 2307 3 0.22522235222007936 2307 4 0.3087160081704283 2307 5 0.2838362336595921 2307 6 0.2484198118849008 2307 7 0.34456168262197906 2307 8 0.3519637215018392 2307 9 0.46634696554338007 2307 10-11 0.40123821008139515 2307 12-13 0.3394833703587494 2307 14-15 0.533717692661547 2307 16-17 0.43454756563467356 2307 18-19 0.4807839795501394 2307 20-21 0.4436493036049356 2307 22-23 0.4001769068105929 2307 24-25 0.44985827644664766 2307 26-27 0.4295779604792287 2307 28-29 0.32909056985852914 2307 30-31 0.2966562571921614 2307 32-33 0.33749251064255503 2307 34-35 0.2534562479917497 2307 36-37 0.2908457355265739 2307 38-39 0.2780471130628328 2307 40-41 0.2971789354738448 2307 42-43 0.25804175873754076 2307 44-45 0.34719653252033567 2307 46-47 0.26385741045151434 2307 48-49 0.2555981154057676 2307 50-51 0.1503301957747496 2307 52-53 0.23209178755270443 2307 54-55 0.26848156951224666 2307 56-57 0.24078110487775461 2307 58-59 0.28180093833703523 2307 60-61 0.29337116621514525 2307 62-63 0.3311480499657904 2307 64-65 0.2936235475834721 2307 66-67 0.20592739443548425 2307 68-69 0.30629742861192 2307 70-71 0.21339541387326477 2307 72-73 0.1860964196662067 2307 74-75 0.2117436053375954 2307 76-77 0.21709652952149128 2307 78-79 0.21442148536507943 2307 80-81 0.22367942411526798 2307 82-83 0.24748323364241998 2307 84-85 0.3192143752139316 2307 86-87 0.41711850336346856 2307 88-89 0.16954067418527075 2307 90-91 0.2644996211949362 2307 92-93 0.20710555874354952 2307 94-95 0.3056506543133253 2307 96-97 0.20035174533915523 2307 98-99 0.10005780793051855 2307 100-101 0.2930527972924999 2201 1 0.22649776835914537 2201 2 0.2808148050459849 2201 3 0.2541002241305961 2201 4 0.28654523179807256 2201 5 0.24924436962461982 2201 6 0.31102211518664546 2201 7 0.3545619072898063 2201 8 0.36114135758274557 2201 9 0.3688525847006119 2201 10-11 0.2983525621577243 2201 12-13 0.3606488253258746 2201 14-15 0.5248851588980514 2201 16-17 0.47108526789502037 2201 18-19 0.48867971112095887 2201 20-21 0.4766000183753434 2201 22-23 0.43920685556932426 2201 24-25 0.4051989216586378 2201 26-27 0.3739647958753096 2201 28-29 0.41043285541420715 2201 30-31 0.3823188782565694 2201 32-33 0.34804433511092014 2201 34-35 0.37147099175093246 2201 36-37 0.41172763463031004 2201 38-39 0.33034329436315346 2201 40-41 0.3243067703052205 2201 42-43 0.3819036394006119 2201 44-45 0.28943013711695187 2201 46-47 0.3190217316964379 2201 48-49 0.36737944575975945 2201 50-51 0.3115414919299937 2201 52-53 0.37912229198390435 2201 54-55 0.4088216718515767 2201 56-57 0.4251412880455874 2201 58-59 0.4561946865205684 2201 60-61 0.425628104937843 2201 62-63 0.3822928310171463 2201 64-65 0.4024381884787971 2201 66-67 0.4081619173954252 2201 68-69 0.364032495831907 2201 70-71 0.3606964595453732 2201 72-73 0.4096785933569933 2201 74-75 0.34810704234006096 2201 76-77 0.28095522724112953 2201 78-79 0.3732597163825062 2201 80-81 0.3685037978716039 2201 82-83 0.42964498391759065 2201 84-85 0.4744156726661757 2201 86-87 0.4694754613395702 2201 88-89 0.41792707134482754 2201 90-91 0.3191112685301718 2201 92-93 0.3955985111106557 2201 94-95 0.5127809220019088 2201 96-97 0.4325075477349358 2201 98-99 0.43605378324355115 2201 100-101 0.2958699618072629 1101 1 0.12575144975137675 1101 2 0.1338362403808162 1101 3 0.11803955870465899 1101 4 0.1805452970999326 1101 5 0.14620195606795505 1101 6 0.2078834630169908 1101 7 0.23231805382717852 1101 8 0.23500396247385424 1101 9 0.2601799265884921 1101 10-11 0.29843382215707237 1101 12-13 0.2806726196903284 1101 14-15 0.386991756018233 1101 16-17 0.30833479445654177 1101 18-19 0.4071937673458592 1101 20-21 0.3375211010801493 1101 22-23 0.3115845370268602 1101 24-25 0.30394896247230463 1101 26-27 0.2098424120309872 1101 28-29 0.23554443996378183 1101 30-31 0.3304887528770024 1101 32-33 0.20255799952467868 1101 34-35 0.21765768976248978 1101 36-37 0.20913270205447532 1101 38-39 0.1246907655487064 1101 40-41 0.1063402293446245 1101 42-43 0.15014159120783432 1101 44-45 0.2227878362059883 1101 46-47 0.1953089537554007 1101 48-49 0.1956692309166428 1101 50-51 0.17451386924413015 1101 52-53 0.12927073126950006 1101 54-55 0.38454297168505747 1101 56-57 0.39865085476776585 1101 58-59 0.3810669805748361 1101 60-61 0.38840196460414944 1101 62-63 0.37847324372489055 1101 64-65 0.42331429452901403 1101 66-67 0.41623918581870356 1101 68-69 0.33495659874763817 1101 70-71 0.3564409007247207 1101 72-73 0.15734756191414334 1101 74-75 0.23031118411039841 1101 76-77 0.2878838767987091 1101 78-79 0.35367009774535063 1101 80-81 0.4023239565551151 1101 82-83 0.37523131297365353 1101 84-85 0.4868533910192596 1101 86-87 0.4132634588581041 1101 88-89 0.420726031412741 1101 90-91 0.35611158687672884 1101 92-93 0.41120153295412365 1101 94-95 0.49938093995992006 1101 96-97 0.32015337494622287 1101 98-99 0.3972325634048488 1101 100-101 0.34141510987922885 2304 1 0.10570004210863004 2304 2 0.0480461512448187 2304 3 0.04583851731378985 2304 4 0.06514330257164858 2304 5 0.10169043899090724 2304 6 0.07595402166141696 2304 7 0.1456321920321031 2304 8 0.11613801948558944 2304 9 0.1741396388821954 2304 10-11 0.10731840868960063 2304 12-13 0.1637571885721556 2304 14-15 0.24231972795363532 2304 16-17 0.31016506098200836 2304 18-19 0.3528305160882468 2304 20-21 0.27196731059387247 2304 22-23 0.21411994872926954 2304 24-25 0.264969393149201 2304 26-27 0.14496872480126655 2304 28-29 0.20360087805397598 2304 30-31 0.23337828160350682 2304 32-33 0.12585956161098721 2304 34-35 0.17459176442491753 2304 36-37 0.1584200553330959 2304 38-39 0.11975386677543298 2304 40-41 0.18730583255705113 2304 42-43 0.2869535451395322 2304 44-45 0.1363412516150433 2304 46-47 0.15195808004611422 2304 48-49 0.20091755410083323 2304 50-51 0.13704894999604988 2304 52-53 0.1552987738076652 2304 54-55 0.27599683718678136 2304 56-57 0.3117072928579532 2304 58-59 0.19414581298150324 2304 60-61 0.10966443040593532 2304 62-63 0.09992964651369363 2304 64-65 0.24980864630732214 2304 66-67 0.39752746575869224 2304 68-69 0.23899397408961676 2304 70-71 0.18317772889523098 2304 72-73 0.24783405913416345 2304 74-75 0.28326447596460014 2304 76-77 0.23432075350862647 2304 78-79 0.2533569082651681 2304 80-81 0.2465627704285609 2304 82-83 0.19102051957501232 2304 84-85 0.37267194189738007 2304 86-87 0.3339734579755742 2304 88-89 0.29765801005540027 2304 90-91 0.34303341637103557 2304 92-93 0.23068491923953616 2304 94-95 0.2722289895583643 2304 96-97 0.2688914607285895 2304 98-99 0.23401285640634484 2304 100-101 0.2599861759810729 2202 1 0.09358924986305084 2202 2 0.11030888136350825 2202 3 0.0831372694807655 2202 4 0.1395793562847416 2202 5 0.12330413362239057 2202 6 0.10336503755635817 2202 7 0.12300063339166911 2202 8 0.1529715783309129 2202 9 0.20140131128977856 2202 10-11 0.17890353181648067 2202 12-13 0.17179838741785147 2202 14-15 0.271495524862452 2202 16-17 0.26177630757770487 2202 18-19 0.3287096802436764 2202 20-21 0.3563429882939957 2202 22-23 0.2900204666751023 2202 24-25 0.2631882142869273 2202 26-27 0.24661865435371766 2202 28-29 0.2530332730179481 2202 30-31 0.32741295723210584 2202 32-33 0.07652784877091534 2202 34-35 0.20357058758493451 2202 36-37 0.21858272997184258 2202 38-39 0.22127647185803312 2202 40-41 0.24217007300737947 2202 42-43 0.2244946062832156 2202 44-45 0.24509311258790234 2202 46-47 0.22611088005356095 2202 48-49 0.22509064353528885 2202 50-51 0.18476512552553714 2202 52-53 0.18442078710422294 2202 54-55 0.3442938092060004 2202 56-57 0.38006766102124345 2202 58-59 0.31272317822977413 2202 60-61 0.3533796307124675 2202 62-63 0.37047352289852853 2202 64-65 0.47634751619289517 2202 66-67 0.4361037866003912 2202 68-69 0.42857608506812284 2202 70-71 0.3993387231702812 2202 72-73 0.4060787176930418 2202 74-75 0.38749362408805865 2202 76-77 0.31987787456532146 2202 78-79 0.26929056117360517 2202 80-81 0.39581641886667285 2202 82-83 0.45947614826120997 2202 84-85 0.36592095104159483 2202 86-87 0.296702878176923 2202 88-89 0.3247970811056504 2202 90-91 0.36110111786500454 2202 92-93 0.41164332524893155 2202 94-95 0.4138791253312206 2202 96-97 0.31216970660450727 2202 98-99 0.35039141320942946 2202 100-101 0.5871179295330506 1102 1 0.12880109094686532 1102 2 0.050052429955776745 1102 3 0.04559968803200576 1102 4 0.078611695648938 1102 5 0.040897957960552844 1102 6 -0.0019988412176914494 1102 7 0.10101038881653324 1102 8 0.10738232380182211 1102 9 0.10088274970969735 1102 10-11 0.1513660633052467 1102 12-13 0.14277290752252014 1102 14-15 0.12611199495838576 1102 16-17 0.08518017336987782 1102 18-19 0.16770240231155498 1102 20-21 0.07762125091060312 1102 22-23 0.1583289775283987 1102 24-25 0.18120213651220496 1102 26-27 0.2561896046814027 1102 28-29 0.14871552268596844 1102 30-31 0.20709184017295712 1102 32-33 0.2137503345140388 1102 34-35 0.2073288513428011 1102 36-37 0.15831849181869018 1102 38-39 0.1387322449273043 1102 40-41 0.10255375745754947 1102 42-43 0.2374792420240226 1102 44-45 0.29838607372669657 1102 46-47 0.2497095333626902 1102 48-49 0.19108806618200447 1102 50-51 0.1256009286243014 1102 52-53 0.2573451142045968 1102 54-55 0.34895833719251357 1102 56-57 0.3583123562266337 1102 58-59 0.3152842150200641 1102 60-61 0.349948766906806 1102 62-63 0.2866388063776739 1102 64-65 0.32177518089444135 1102 66-67 0.4964262767421346 1102 68-69 0.3583003807837528 1102 70-71 0.2872038531732741 1102 72-73 0.32191877080934717 1102 74-75 0.2879329213550861 1102 76-77 0.33239052059651897 1102 78-79 0.3520861335469476 1102 80-81 0.4403390973168868 1102 82-83 0.4311482359292569 1102 84-85 0.41747479311688807 1102 86-87 0.3596253388360253 1102 88-89 0.39378247412230394 1102 90-91 0.40148448727889985 1102 92-93 0.363088509533263 1102 94-95 0.33856584257011946 1102 96-97 0.33341839742078605 1102 98-99 0.387567119039268 1102 100-101 0.33496973885170434 2305 1 0.09426553531367432 2305 2 0.08966994833016884 2305 3 0.11031722409763489 2305 4 0.05857481684219579 2305 5 0.104071140154268 2305 6 0.13365161316309582 2305 7 0.11431119835403791 2305 8 0.1756505497219223 2305 9 0.0729556439781689 2305 10-11 0.09170656134940458 2305 12-13 0.11321410841438251 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70-71 0.11543890172422522 1213 72-73 0.08574711213903896 1213 74-75 0.09543757924615903 1213 76-77 0.08736184614737397 1213 78-79 0.02727350437537268 1213 80-81 0.1599551409629143 1213 82-83 0.1651109564852291 1213 84-85 0.22757969453417104 1213 86-87 0.21123863793523157 1213 88-89 0.09031394578888552 1213 90-91 0.10080111711160811 1213 92-93 0.1782074050180924 1213 94-95 0.2709553610826916 1213 96-97 0.21066499413662854 1213 98-99 0.13177320974834572 1213 100-101 0.18916602608778987 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2341.0 3 405.0 4 446.0 5 455.0 6 1163.0 7 2842.0 8 8909.0 9 10586.0 10 10572.0 11 9077.0 12 7277.0 13 6687.0 14 6738.0 15 7178.0 16 7723.0 17 8436.0 18 9378.0 19 10716.0 20 12072.0 21 14191.0 22 16460.0 23 18559.0 24 21394.0 25 25011.0 26 30100.0 27 36496.0 28 44693.0 29 54823.0 30 67455.0 31 86888.0 32 119444.0 33 170886.0 34 260476.0 35 436466.0 36 874738.0 37 1659902.0 38 618063.0 39 639.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.03652383714425 32.7865282313052 16.133387006401755 18.043560925148793 2 30.762571100820608 25.46336744715508 30.01193415875967 13.762127293264648 3 31.936345784786006 23.842158866887956 25.2184870673052 19.003008281020836 4 28.100961378836725 23.355522953372237 29.567138541499986 18.976377126291048 5 29.1202638850597 21.75241581519477 30.667512408171817 18.459807891573714 6 27.308549818145693 22.00551675763843 32.07624370596962 18.60968971824626 7 31.54710905760909 22.664152750984723 25.359969880955717 20.428768310450465 8 27.56034469104982 27.018056855787865 25.17414551729127 20.247452935871042 9 25.92069998170444 24.8129305742345 28.53563835990469 20.730731084156364 10-11 22.731442802142492 29.008385759933418 30.961671974403053 17.298499463521036 12-13 22.825279462745023 28.527182943412814 30.018000447041953 18.62953714680021 14-15 22.25394139119355 28.531404973357393 30.714720298135223 18.499933337313834 16-17 22.279449252397097 28.400254434821402 30.980039491964924 18.340256820816577 18-19 21.881335921406116 28.360033531974764 31.07813044263405 18.680500103985068 20-21 21.96982567887836 28.93588753818383 30.505832529500726 18.58845425343708 22-23 22.155600829136514 28.985895925152978 30.264884694524795 18.593618551185717 24-25 21.61572470080824 29.30868052129942 30.32487607069908 18.75071870719326 26-27 21.34684289221046 29.36368225685439 30.51622412876924 18.773250722165905 28-29 21.118971724809025 29.655830882237222 30.368210968741206 18.85698642421255 30-31 21.105396121247143 29.621661050038643 30.51798871415729 18.754954114556917 32-33 20.645896876490863 29.666604128585444 30.970598167812902 18.716900827110795 34-35 20.641180267981696 30.179629072296905 30.665056544929353 18.514134114792043 36-37 20.629917275435822 29.896530774303336 30.897860040753876 18.57569190950696 38-39 20.8629852364749 29.27007508223729 30.920439032041802 18.94650064924601 40-41 20.93841960427169 29.62732164059707 30.17676559195308 19.257493163178154 42-43 21.351009719202775 29.244658448912258 30.372985853443968 19.031345978440996 44-45 20.767107332329555 29.46710574560725 30.566178971126373 19.19960795093683 46-47 21.064687229106248 29.65823583481706 30.18023910924023 19.096837826836456 48-49 20.783684911622817 29.90455815268144 30.137991739860837 19.17376519583491 50-51 20.892440790313913 29.41000544852538 30.048397617747284 19.649156143413425 52-53 21.2141908271478 29.261338476895247 29.851804517485505 19.67266617847145 54-55 21.401599172517958 29.45661969553927 29.705580464974062 19.436200666968713 56-57 21.351428751248687 29.215288747532508 29.925104920979706 19.508177580239096 58-59 21.37434083334468 29.479098197512148 29.916364486635587 19.23019648250758 60-61 20.841519131159444 29.681709169933573 29.61768764721172 19.859084051695262 62-63 20.952554670601394 29.678513254146505 29.482552058857163 19.886380016394938 64-65 20.72392702872486 29.64292187355806 29.79524994096912 19.83790115674796 66-67 20.94347852793817 29.18287312060432 29.861686940568465 20.011961410889043 68-69 20.743759965597654 29.301761834060123 30.434940591211024 19.5195376091312 70-71 20.73171372077624 30.16790135681425 29.846859413999326 19.253525508410178 72-73 20.64960299157975 30.38833270180047 29.533855876770502 19.428208429849274 74-75 20.79732345372534 30.018374253586128 29.499977712591875 19.68432458009666 76-77 20.931215883488395 29.823695147571495 29.538215314928983 19.70687365401113 78-79 20.650807575276637 29.680767772517942 29.982005063701344 19.686419588504073 80-81 20.606246244881625 29.966782407729927 29.702780036177746 19.724191311210706 82-83 20.29358666768005 30.012566879802293 29.645937300242302 20.04790915227536 84-85 20.372428200784302 29.965951412295546 29.72329694087937 19.938323446040787 86-87 20.449710877945957 29.85603656513674 30.11002663170797 19.58422592520933 88-89 20.64447432638078 30.25150494759965 29.70940650672494 19.39461421929463 90-91 20.93358511575299 29.924787916330402 29.363090307988266 19.77853665992834 92-93 20.56873375766362 30.206381158423607 29.33673663497318 19.888148448939596 94-95 20.147211424381556 30.56395645599062 29.338902093493314 19.949930026134506 96-97 20.372256821449977 30.707275527327386 29.157748897926705 19.76271875329593 98-99 20.595120578098268 30.32068083241674 29.033459667895567 20.050738921589424 100-101 20.59787238444365 30.195242777590842 28.97016104991324 20.236723788052263 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1425.0 1 1085.0 2 550.5 3 373.5 4 402.0 5 508.0 6 695.0 7 937.5 8 1187.0 9 1404.5 10 1620.0 11 1697.5 12 1537.5 13 1287.5 14 1102.5 15 942.5 16 797.0 17 771.5 18 892.5 19 1126.0 20 1456.5 21 1978.5 22 2985.0 23 4604.0 24 6742.0 25 9744.5 26 14438.0 27 20502.5 28 27289.0 29 34548.0 30 42570.5 31 51904.5 32 66441.0 33 89734.0 34 118243.0 35 155813.0 36 203699.0 37 259910.5 38 312856.5 39 329682.5 40 334907.0 41 321328.5 42 285797.5 43 271428.0 44 254610.0 45 238297.0 46 240226.0 47 223435.0 48 177325.0 49 132339.5 50 100665.0 51 81000.5 52 64412.5 53 48497.0 54 37697.0 55 29117.0 56 20192.5 57 13249.5 58 8700.0 59 6051.0 60 4469.5 61 3288.0 62 2278.0 63 1514.0 64 1026.0 65 760.5 66 557.5 67 387.5 68 292.0 69 227.0 70 185.5 71 138.0 72 89.5 73 78.0 74 72.5 75 49.0 76 38.5 77 34.5 78 27.5 79 21.0 80 20.0 81 16.5 82 11.0 83 9.5 84 8.0 85 5.0 86 6.5 87 6.5 88 4.5 89 5.0 90 2.5 91 1.5 92 2.5 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3077771260245081 2 0.2830959776138779 3 0.2543547268672998 4 0.24117007875530083 5 0.24770898041214315 6 0.19633800138257168 7 0.16150659713207194 8 0.15904916677084033 9 0.13718872103571075 10-11 0.12158938048180594 12-13 0.14414431740597924 14-15 0.147574035431872 16-17 0.14066117698092928 18-19 0.12686751351853812 20-21 0.13336367725605464 22-23 0.16230793311943004 24-25 0.5819622474589636 26-27 0.2295881026180181 28-29 0.2029089564789083 30-31 0.3070933193152958 32-33 0.338911700253329 34-35 0.3476622892352797 36-37 0.3383667917819255 38-39 0.34614509309921504 40-41 0.3490405871335357 42-43 0.360152446158235 44-45 0.34154008229186367 46-47 0.3606866701498071 48-49 0.35661588333402783 50-51 0.36232139556401766 52-53 0.3472455945218535 54-55 0.3397237207205186 56-57 0.3481965132268518 58-59 0.34054642566753957 60-61 0.3172756285946597 62-63 0.3038772908860318 64-65 0.31526694638634867 66-67 0.33163556948811723 68-69 0.3181197025013435 70-71 0.29787261322076164 72-73 0.29465658479149776 74-75 0.2857670975717383 76-77 0.28860916920690177 78-79 0.2976268701846385 80-81 0.30741385371023905 82-83 0.32994742167474944 84-85 0.3211220413339787 86-87 0.3233657820985814 88-89 0.32914608568739134 90-91 0.32214775139779706 92-93 0.30377044608771747 94-95 0.2994752851954779 96-97 0.3171367303568509 98-99 0.32520351262958935 100-101 0.3261223778950934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4679685.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.11733951414285 #Duplication Level Percentage of deduplicated Percentage of total 1 90.96886935101637 46.500865798336065 2 4.233406524798559 4.32800957259031 3 1.3650466936031294 2.093326658687079 4 0.7124075809017241 1.4566552074161045 5 0.46954457369969865 1.200093469541548 6 0.2635494084047796 0.8083166752907166 7 0.2198357970085759 0.7866194751134694 8 0.18428954860017446 0.7536313139762597 9 0.14683918840323493 0.6755425782830411 >10 1.156873220263169 12.25348668740775 >50 0.15021443878803684 5.306412745350455 >100 0.10246616100421897 10.13492026788134 >500 0.016633540191361887 6.085133242728655 >1k 0.01002397331730649 7.616986307396995 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 7.479135882009153E-4 0.0 0.0 2 0.0010043411041555147 0.0 0.0 3 0.0014958271764018306 0.0 0.0 4 0.002265109724265629 0.0 0.0 5 0.0027993337158377114 0.0 0.0 6 0.004936229682126041 0.0 0.0 7 0.0061115224635846215 0.0 0.0 8 0.007906515075266818 0.0 0.0 9 0.008974963058410983 0.0 0.0 10-11 0.01277863787840421 0.0 0.0 12-13 0.018804684503337298 0.0 0.0 14-15 0.02739500628781638 0.0 0.0 16-17 0.03825043779656109 0.0 2.136895966288329E-5 18-19 0.051873149581649194 0.0 2.136895966288329E-5 20-21 0.0663933576725784 0.0 3.205343949432494E-5 22-23 0.08439670618855757 0.0 4.273791932576658E-5 24-25 0.10426983867503903 0.0 4.273791932576658E-5 26-27 0.12495499162871006 0.0 4.273791932576658E-5 28-29 0.14612094617479593 0.0 4.273791932576658E-5 30-31 0.16992596723924794 0.0 4.273791932576658E-5 32-33 0.19361345902555407 0.0 4.273791932576658E-5 34-35 0.21959811397562015 0.0 4.273791932576658E-5 36-37 0.24832868024236673 0.0 4.273791932576658E-5 38-39 0.2805958093333205 0.0 4.273791932576658E-5 40-41 0.3159828065350552 0.0 4.273791932576658E-5 42-43 0.35409434609380763 0.0 4.273791932576658E-5 44-45 0.3961377742305304 0.0 4.273791932576658E-5 46-47 0.4429892182914021 0.0 4.273791932576658E-5 48-49 0.49354817685378394 0.0 4.273791932576658E-5 50-51 0.546842362253015 0.0 4.273791932576658E-5 52-53 0.6021665988202198 0.0 4.273791932576658E-5 54-55 0.658975978083995 0.0 4.273791932576658E-5 56-57 0.7207002180702333 0.0 4.273791932576658E-5 58-59 0.7863990845537681 0.0 4.273791932576658E-5 60-61 0.8535510402943788 0.0 5.342239915720823E-5 62-63 0.9250302103667234 0.0 8.547583865153317E-5 64-65 1.001755460036306 0.0 8.547583865153317E-5 66-67 1.0793888904915607 0.0 8.547583865153317E-5 68-69 1.1601315045777654 0.0 8.547583865153317E-5 70-71 1.2458210328259274 0.0 8.547583865153317E-5 72-73 1.3346517981445332 0.0 8.547583865153317E-5 74-75 1.4275106123595926 0.0 9.616031848297482E-5 76-77 1.5239700962778477 0.0 1.0684479831441647E-4 78-79 1.6259641407487897 0.0 1.0684479831441647E-4 80-81 1.7335247992119127 0.0 1.2821375797729975E-4 82-83 1.8479235247671584 0.0 1.2821375797729975E-4 84-85 1.9665319353759922 0.0 1.2821375797729975E-4 86-87 2.0910381788517816 0.0 1.7095167730306633E-4 88-89 2.223493675322164 0.0 1.7095167730306633E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 885 0.0 58.99901 2 GAGCGGC 530 0.0 49.193783 9 CGGGAGA 860 0.0 48.00441 4 CGGTGCG 50 2.8366081E-5 47.476532 1 AGAGCGG 570 0.0 47.40593 8 GGTCCGG 175 0.0 43.40712 1 CGAACCG 45 9.651548E-4 42.201366 1 GAGAGCG 995 0.0 41.451305 7 CGACGGG 105 1.033186E-9 40.69417 1 CGAGGCG 155 0.0 39.81903 1 GATCGGG 1660 0.0 36.32241 1 GGTACGG 145 1.6370905E-11 36.01668 1 CGGAAAG 500 0.0 35.114952 5 AAGCGGC 280 0.0 33.860653 9 CGGGGCC 255 0.0 33.51285 1 AACGCGG 85 1.6388014E-5 33.51285 1 ATTCGGA 220 0.0 32.364124 2 GGACCGG 225 0.0 31.651024 1 CGGACCC 90 2.4227025E-5 31.651024 1 GAACCGG 240 0.0 31.651022 1 >>END_MODULE