FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_34271-1.342000000358da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_34271-1.342000000358da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5261404
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC46200.076.244439
ATTCGGA10800.071.639832
ATCCGGA14250.070.95052
ATCGGGA75300.069.4668052
CGGGAGA69200.068.568934
TCGGGAG70650.067.838433
CGGAAAG29750.065.552365
TCGGAGA17100.065.486144
GTTCGGA12500.065.314432
AGAGCGG55850.065.281378
GATCGGG84150.064.3143161
AAGCGGC13750.062.407559
GATCCGG17200.062.1027681
TCGGGAA20450.058.9395683
CGGAGAG37750.057.9451945
TCGGAAA20700.057.5356254
ATCGGAG13950.057.4882473
GAGAGCG66800.057.2779247
GGATCGG28250.056.9689451
ACCGGAA10250.056.018063