##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_34271-1.342000000358da.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5261404 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.004762226964512 33.0 33.0 33.0 27.0 33.0 2 30.54936191936601 33.0 33.0 33.0 27.0 33.0 3 30.39442931962647 33.0 33.0 33.0 22.0 33.0 4 33.83015008921573 37.0 37.0 37.0 22.0 37.0 5 33.88916684595975 37.0 37.0 37.0 27.0 37.0 6 34.33818748759837 37.0 37.0 37.0 27.0 37.0 7 33.96913561475226 37.0 37.0 37.0 22.0 37.0 8 34.02524592295136 37.0 37.0 37.0 27.0 37.0 9 33.62663254903064 37.0 37.0 37.0 22.0 37.0 10-11 33.65390701797467 37.0 37.0 37.0 22.0 37.0 12-13 33.630192245263814 37.0 37.0 37.0 18.5 37.0 14-15 35.21781438946715 40.0 37.0 40.0 22.0 40.0 16-17 35.13751405518374 40.0 37.0 40.0 18.5 40.0 18-19 35.15633013545434 40.0 37.0 40.0 18.5 40.0 20-21 35.12405738088161 40.0 37.0 40.0 18.5 40.0 22-23 35.739463743898014 40.0 37.0 40.0 24.5 40.0 24-25 35.14249723077718 40.0 37.0 40.0 22.0 40.0 26-27 34.91677801210476 38.5 37.0 40.0 18.5 40.0 28-29 34.725071387789264 37.0 37.0 40.0 15.0 40.0 30-31 34.73679477949232 37.0 37.0 40.0 15.0 40.0 32-33 34.57112531179891 37.0 37.0 40.0 15.0 40.0 34-35 34.522316191647704 37.0 37.0 40.0 15.0 40.0 36-37 34.38758884510675 37.0 37.0 40.0 15.0 40.0 38-39 34.31807222938973 37.0 37.0 40.0 15.0 40.0 40-41 34.28080879172175 37.0 37.0 40.0 15.0 40.0 42-43 34.03004749302657 37.0 33.0 40.0 15.0 40.0 44-45 33.88298503593337 37.0 33.0 40.0 15.0 40.0 46-47 33.792244047406356 37.0 33.0 40.0 15.0 40.0 48-49 33.681039604637846 37.0 33.0 40.0 15.0 40.0 50-51 32.675237636189884 37.0 33.0 38.5 15.0 38.5 52-53 33.093646201660235 37.0 33.0 38.5 15.0 40.0 54-55 33.75543410085977 37.0 33.0 40.0 15.0 40.0 56-57 33.95588088274536 37.0 33.0 40.0 15.0 40.0 58-59 33.5671393035015 37.0 33.0 40.0 15.0 40.0 60-61 33.286354744855174 37.0 33.0 40.0 15.0 40.0 62-63 33.004323465751725 37.0 33.0 40.0 15.0 40.0 64-65 32.788852082067834 37.0 33.0 38.5 15.0 40.0 66-67 32.57145060519967 37.0 33.0 37.0 15.0 40.0 68-69 32.51234204786403 37.0 33.0 37.0 15.0 40.0 70-71 32.19947061658827 37.0 33.0 37.0 15.0 40.0 72-73 32.19683244244312 37.0 33.0 37.0 15.0 38.5 74-75 32.20589722819232 37.0 33.0 37.0 15.0 37.0 76-77 31.984421648670203 37.0 33.0 37.0 15.0 37.0 78-79 31.662883329240636 37.0 33.0 37.0 15.0 37.0 80-81 31.280805940771703 35.0 33.0 37.0 15.0 37.0 82-83 30.998930988762694 33.0 33.0 37.0 15.0 37.0 84-85 30.722486336346726 33.0 27.0 37.0 15.0 37.0 86-87 30.568141412444284 33.0 27.0 37.0 15.0 37.0 88-89 30.44651674724085 33.0 27.0 37.0 15.0 37.0 90-91 30.3116980752666 33.0 27.0 37.0 15.0 37.0 92-93 30.156244797016157 33.0 27.0 37.0 15.0 37.0 94-95 30.011627409718017 33.0 27.0 37.0 15.0 37.0 96-97 29.8563467089773 33.0 27.0 37.0 15.0 37.0 98-99 29.698574848082373 33.0 27.0 37.0 10.5 37.0 100-101 28.451018777497413 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.09925440825167442 2306 2 0.26677147056340544 2306 3 0.17862562380567581 2306 4 0.3541414799935083 2306 5 0.29564313944758425 2306 6 0.10697567277231457 2306 7 0.4638921318451992 2306 8 0.1689054586027865 2306 9 0.3248011503719255 2306 10-11 0.28854959317790474 2306 12-13 0.3186642728509739 2306 14-15 0.5497650559170992 2306 16-17 0.4643595274014132 2306 18-19 0.490271412768557 2306 20-21 0.47856520015653103 2306 22-23 0.26577844716776866 2306 24-25 0.5448438809431551 2306 26-27 0.21467913624506707 2306 28-29 0.2786044680580204 2306 30-31 0.19079111272861127 2306 32-33 0.3731337228758491 2306 34-35 0.3440676519795005 2306 36-37 0.3912412782746699 2306 38-39 0.2796156708512072 2306 40-41 0.3148256429946201 2306 42-43 0.44474041373201345 2306 44-45 0.4856490182038016 2306 46-47 0.49857417052465536 2306 48-49 0.45768255498628463 2306 50-51 0.3617289906250889 2306 52-53 0.37904784404828007 2306 54-55 0.43303668665961226 2306 56-57 0.43513523597611226 2306 58-59 0.5544083124406072 2306 60-61 0.46352839328450557 2306 62-63 0.5058769845832813 2306 64-65 0.4700977458814677 2306 66-67 0.503518177022471 2306 68-69 0.1972826749669494 2306 70-71 0.429385869778919 2306 72-73 0.2822665652728844 2306 74-75 0.22405059621933532 2306 76-77 0.2780241728213717 2306 78-79 0.33776702004341175 2306 80-81 0.2332887188594448 2306 82-83 0.36820269158824104 2306 84-85 0.44243644272803095 2306 86-87 0.4177823836031891 2306 88-89 0.41501568822835466 2306 90-91 0.4065411213350707 2306 92-93 0.3716942939661685 2306 94-95 0.2728371173841069 2306 96-97 0.3406248438170927 2306 98-99 0.3673209862166509 2306 100-101 0.3060763961034425 2307 1 -0.05662149510316539 2307 2 -0.05715604737693525 2307 3 -0.0700620105318599 2307 4 -0.011225828475346589 2307 5 -0.030189323200474405 2307 6 -0.2929812305698505 2307 7 0.13020448845374943 2307 8 -0.14822980876851233 2307 9 0.10410461422053174 2307 10-11 0.017198187013633515 2307 12-13 -0.023359314420659416 2307 14-15 0.22017702737190348 2307 16-17 0.20555051621619214 2307 18-19 0.2319712318712419 2307 20-21 0.11181445275546054 2307 22-23 0.01669877810663678 2307 24-25 0.19978994674342232 2307 26-27 -0.21286506046401854 2307 28-29 -0.1338042910660704 2307 30-31 -0.044356384677442406 2307 32-33 0.011245835752873745 2307 34-35 -0.18405382265051173 2307 36-37 -0.07516143645261053 2307 38-39 -0.16996123948057829 2307 40-41 -0.20765523965390287 2307 42-43 -0.08551688055839435 2307 44-45 0.08885283823360624 2307 46-47 0.04338926232234286 2307 48-49 -0.009635511024029597 2307 50-51 -0.21782961268104373 2307 52-53 -0.22934387197734196 2307 54-55 -0.08635771475859855 2307 56-57 -0.2749319984316898 2307 58-59 0.052515137828521574 2307 60-61 -0.010619949757639802 2307 62-63 -0.03751120946757425 2307 64-65 0.06792353760147307 2307 66-67 0.05213410431587562 2307 68-69 -0.005163875218023861 2307 70-71 -0.09963103751075408 2307 72-73 -0.17870560841026872 2307 74-75 -0.22794642685186517 2307 76-77 -0.21342983236109703 2307 78-79 -0.17105964628524006 2307 80-81 -0.024212000927175126 2307 82-83 -0.028812593239020856 2307 84-85 -0.028374625507741058 2307 86-87 0.039563036101075255 2307 88-89 -0.14267045583398996 2307 90-91 -0.11389629163412351 2307 92-93 -0.036840292912525996 2307 94-95 0.014644678131382705 2307 96-97 -0.03937450430068523 2307 98-99 0.009138868432398084 2307 100-101 -0.04897125467601171 2201 1 0.11987783384925166 2201 2 0.08412665212439663 2201 3 0.030920259258447658 2201 4 0.14668806091738418 2201 5 0.06639770105334719 2201 6 -0.0449548631459038 2201 7 0.265341823649905 2201 8 -0.046611148393623125 2201 9 0.16603166469695907 2201 10-11 0.17771483469834948 2201 12-13 0.14607650778918213 2201 14-15 0.3925900463500014 2201 16-17 0.3320631422601821 2201 18-19 0.30233106796151077 2201 20-21 0.2950895817872734 2201 22-23 0.3131560351328133 2201 24-25 0.49088450996581656 2201 26-27 0.10952941523458293 2201 28-29 0.25126697550899735 2201 30-31 0.28169839386136886 2201 32-33 0.3170263011880081 2201 34-35 0.23391473428337406 2201 36-37 0.2519077440332822 2201 38-39 0.2599462060927493 2201 40-41 0.2416067092388161 2201 42-43 0.32698849514665795 2201 44-45 0.29858384992135 2201 46-47 0.26636341499153815 2201 48-49 0.3029053315316119 2201 50-51 0.16470198681794557 2201 52-53 0.21119928175931335 2201 54-55 0.3414164497725807 2201 56-57 0.3518098878116618 2201 58-59 0.560556781723001 2201 60-61 0.49036158163617927 2201 62-63 0.4856795017904645 2201 64-65 0.5230378145809027 2201 66-67 0.4195683242053434 2201 68-69 0.36079652910407134 2201 70-71 0.41662178372953207 2201 72-73 0.36061421290254003 2201 74-75 0.28457338331354265 2201 76-77 0.1656660797449483 2201 78-79 0.37142828301638886 2201 80-81 0.45285688777301303 2201 82-83 0.4845565813039734 2201 84-85 0.4852667136433624 2201 86-87 0.5059148739282442 2201 88-89 0.5132748327974532 2201 90-91 0.32968140369467136 2201 92-93 0.3533728928717217 2201 94-95 0.5079202680655115 2201 96-97 0.5456526847679939 2201 98-99 0.5506912970243967 2201 100-101 0.47636693984692613 1101 1 0.18228073202319095 1101 2 0.12439799826351461 1101 3 0.08994399465601788 1101 4 0.1509975991503012 1101 5 0.1450265691522219 1101 6 0.1071508653934643 1101 7 0.4046125024723608 1101 8 0.12435404993831867 1101 9 0.20082981651660248 1101 10-11 0.1510813126344317 1101 12-13 0.20532245315097697 1101 14-15 0.3779216614134455 1101 16-17 0.276604051364572 1101 18-19 0.33452923408786717 1101 20-21 0.2697846220301017 1101 22-23 0.25787836030886524 1101 24-25 0.2628598639716486 1101 26-27 0.12620098632523735 1101 28-29 0.2500837603305257 1101 30-31 0.21319698730993508 1101 32-33 0.19101223644278775 1101 34-35 0.19445701340325883 1101 36-37 0.1992165118797189 1101 38-39 0.19011033104809627 1101 40-41 0.15135537271002875 1101 42-43 0.27658075950383676 1101 44-45 0.2604802390382801 1101 46-47 0.23521498422957166 1101 48-49 0.18955281197444407 1101 50-51 0.1703197316172833 1101 52-53 0.1585164556199672 1101 54-55 0.279325123537582 1101 56-57 0.2905101553866345 1101 58-59 0.5085522055547855 1101 60-61 0.4307440409985901 1101 62-63 0.45213813150905224 1101 64-65 0.46133681859458875 1101 66-67 0.5347982693508797 1101 68-69 0.38959935225021525 1101 70-71 0.37953230626825274 1101 72-73 0.07307004149545548 1101 74-75 0.11044060103381526 1101 76-77 0.24271571599528485 1101 78-79 0.22194001149400222 1101 80-81 0.29778221544549055 1101 82-83 0.41654102039811747 1101 84-85 0.42492510201594413 1101 86-87 0.34014500444562756 1101 88-89 0.44991672571318375 1101 90-91 0.32018524480364263 1101 92-93 0.3190192708630377 1101 94-95 0.4337774644982417 1101 96-97 0.37888879036819034 1101 98-99 0.23720157812920206 1101 100-101 0.2763763787570106 2304 1 0.11441159597510264 2304 2 0.006211834366499858 2304 3 -0.034551986446174965 2304 4 0.010522126415416722 2304 5 -0.04806859241754324 2304 6 0.05270916468375475 2304 7 0.2752505160420071 2304 8 0.040203164617729215 2304 9 0.11398264993253093 2304 10-11 0.0823432740597454 2304 12-13 0.14180105489241868 2304 14-15 0.2799122028355967 2304 16-17 0.1822305032519438 2304 18-19 0.23259642296785188 2304 20-21 0.17360377681936967 2304 22-23 0.15479003607098463 2304 24-25 0.24200690883629505 2304 26-27 0.09155988224933509 2304 28-29 0.057262989032324185 2304 30-31 0.19557037295530932 2304 32-33 0.1993052729569058 2304 34-35 0.09498434161605473 2304 36-37 0.11980549803059404 2304 38-39 0.1633307476193906 2304 40-41 0.19517597781188556 2304 42-43 0.21244562403365563 2304 44-45 0.21444579073809678 2304 46-47 0.11430870314649155 2304 48-49 0.14778587569477253 2304 50-51 0.15932796977574526 2304 52-53 0.165312943908269 2304 54-55 0.3844053094960813 2304 56-57 0.2928410868854243 2304 58-59 0.13981516980221187 2304 60-61 0.15676268401598747 2304 62-63 0.1608905210225018 2304 64-65 0.2784460775251745 2304 66-67 0.31630149768218985 2304 68-69 0.2926728155714713 2304 70-71 0.3622203714454386 2304 72-73 0.2804966649006744 2304 74-75 0.4508343311682381 2304 76-77 0.368808754303398 2304 78-79 0.3840489020512372 2304 80-81 0.2702400336734492 2304 82-83 0.240276968164288 2304 84-85 0.3484605710277826 2304 86-87 0.26311428210832233 2304 88-89 0.15747613486047385 2304 90-91 0.2335558518758667 2304 92-93 0.20524832107761526 2304 94-95 0.23719943621986772 2304 96-97 0.2186641975215302 2304 98-99 0.11932346707516928 2304 100-101 0.08787106848637194 2202 1 0.04900342750471509 2202 2 -0.0908203513211987 2202 3 3.6872706099444486E-4 2202 4 0.014056618421506073 2202 5 -0.07690563633923375 2202 6 0.08324087815567083 2202 7 0.181151112931758 2202 8 0.07462071889253963 2202 9 0.015624396808370022 2202 10-11 0.058503972203965304 2202 12-13 0.06983001533506439 2202 14-15 0.14123088510226012 2202 16-17 0.10837847707490766 2202 18-19 0.12081940258568835 2202 20-21 0.16417751149486293 2202 22-23 0.094846011832324 2202 24-25 0.05731718348659598 2202 26-27 0.10020032462437456 2202 28-29 -0.0028968423202542226 2202 30-31 0.05123939094901431 2202 32-33 -0.08085596304071174 2202 34-35 -0.11253367872335929 2202 36-37 0.013151212494200593 2202 38-39 -0.05281064931416779 2202 40-41 -0.06177724957107955 2202 42-43 -0.09248771815138213 2202 44-45 -0.039263766174229886 2202 46-47 0.00332027187057804 2202 48-49 0.04847939339855145 2202 50-51 -0.07846085450071882 2202 52-53 0.08324501009798979 2202 54-55 0.08975555370552968 2202 56-57 0.1650239202851438 2202 58-59 0.10981132343774647 2202 60-61 0.11065619504348945 2202 62-63 0.1638576695818088 2202 64-65 0.07982340794194442 2202 66-67 0.1695761959929385 2202 68-69 0.16926044891897618 2202 70-71 0.18374118382161697 2202 72-73 0.16769438491234467 2202 74-75 0.20239974149337314 2202 76-77 0.15416989623292565 2202 78-79 0.13567994999520394 2202 80-81 0.10599239456483289 2202 82-83 0.23040380442355968 2202 84-85 0.3302944223356974 2202 86-87 0.28803923017958866 2202 88-89 0.2162469335465218 2202 90-91 0.16760129711271432 2202 92-93 0.13480335615811256 2202 94-95 0.10853587252474739 2202 96-97 0.11127139500524308 2202 98-99 0.051044402925086274 2202 100-101 0.042141245677932204 1102 1 0.07066552729247633 1102 2 -0.08947244324656367 1102 3 -0.06520869412188901 1102 4 -0.04213093731708284 1102 5 -0.10541827298289519 1102 6 -8.51586633970669E-4 1102 7 0.013771475944444944 1102 8 0.13433411723426758 1102 9 0.03202334054137168 1102 10-11 0.0324170457018198 1102 12-13 0.008378276605746748 1102 14-15 0.015875259363518524 1102 16-17 0.0067528287068654436 1102 18-19 -0.03521110623967871 1102 20-21 -0.03691077405870402 1102 22-23 0.06068942253923382 1102 24-25 -0.08072193167391362 1102 26-27 0.08543215519934932 1102 28-29 -0.00977816540532217 1102 30-31 -0.025469513520938847 1102 32-33 -0.045121213520445735 1102 34-35 -0.05587679140035107 1102 36-37 -0.09400964255977584 1102 38-39 -0.1025219098811263 1102 40-41 -0.19384066806698286 1102 42-43 -0.07029117466925783 1102 44-45 -0.062306504889207304 1102 46-47 -0.10388104883025306 1102 48-49 -0.042820610516919544 1102 50-51 0.0064190953743192836 1102 52-53 0.05719942323074889 1102 54-55 0.11665242678602539 1102 56-57 0.2207876355174605 1102 58-59 0.1507157341408174 1102 60-61 0.04319052267224066 1102 62-63 0.14696747901395923 1102 64-65 0.1289151202278731 1102 66-67 0.18163447806104926 1102 68-69 0.21494566167039153 1102 70-71 0.21968201972477175 1102 72-73 0.23814608116584424 1102 74-75 0.2363810306327423 1102 76-77 0.21029351974544497 1102 78-79 0.2478335273506609 1102 80-81 0.1183101199864538 1102 82-83 0.1744123060549434 1102 84-85 0.29597680074385835 1102 86-87 0.20198396302066968 1102 88-89 0.09690972063848591 1102 90-91 0.07144382054695342 1102 92-93 0.10635598457229989 1102 94-95 0.10645108327298303 1102 96-97 0.053120031811118906 1102 98-99 0.11173282233866999 1102 100-101 0.021381800751242963 2305 1 0.0892164312468644 2305 2 -0.018750265124367615 2305 3 0.047542954038998886 2305 4 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Count 2 2449.0 3 805.0 4 836.0 5 755.0 6 1827.0 7 15719.0 8 89255.0 9 126139.0 10 98170.0 11 62003.0 12 37951.0 13 25563.0 14 19426.0 15 16682.0 16 15484.0 17 14940.0 18 15362.0 19 16643.0 20 18173.0 21 19986.0 22 22394.0 23 25248.0 24 28754.0 25 33468.0 26 39792.0 27 47048.0 28 56063.0 29 68467.0 30 84725.0 31 109441.0 32 147223.0 33 210572.0 34 318561.0 35 527885.0 36 968681.0 37 1521754.0 38 452037.0 39 1123.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.60694269701475 23.750600480384307 21.56229745701323 15.080159365587708 2 32.907789388623236 25.18924111065159 29.117935738639723 12.785033762085455 3 35.669617220595725 21.05503928165463 27.46177674180043 15.813566755949216 4 29.101757210805285 19.02576616383806 32.661347858547565 19.211128766809082 5 33.3897898863642 17.343738866659784 31.101089490812694 18.16538175616332 6 31.104351967357058 17.458064734807493 34.901466955930765 16.536116341904684 7 36.17771329280476 18.466213664384394 27.28135050924685 18.074722533563996 8 29.279190190397316 29.134007552847375 24.06902371291033 17.517778543844987 9 31.68575108648736 25.93294418473292 24.062431250033303 18.31887347874641 10-11 27.109080121811154 28.722341508117065 27.77954000382087 16.38903836625091 12-13 28.947613840855475 24.314756606913956 27.471334591385293 19.266294960845272 14-15 26.47504504886023 24.55229736101945 28.51492215242907 20.45773543769125 16-17 27.63393776873554 24.83016699012866 29.199934720786242 18.33596052034956 18-19 27.36135878259741 24.900947791812726 29.433096539200243 18.304596886389625 20-21 26.202301352970224 26.610295286061046 28.664604399650983 18.522798961317744 22-23 28.504947143913384 25.097979643382274 27.620589012421544 18.776484200282802 24-25 25.608905630414885 27.605340251159205 27.893372739251276 18.892381379174633 26-27 23.149443186445037 30.15875555682546 27.84635422247303 18.845447034256473 28-29 24.453413755610594 29.07719321732687 27.34538975575761 19.124003271304925 30-31 26.834378414256932 26.60000604278501 27.610504571595612 18.95511097136244 32-33 24.26193415682981 26.27418964089245 30.508920745702284 18.954955456575465 34-35 21.668096446185032 28.350158391346913 29.278385175832366 20.703359986635686 36-37 23.727351115440047 29.60148336345494 27.811196083822846 18.859969437282167 38-39 25.337401838741453 27.1415599791642 27.51698010157978 20.004058080514568 40-41 23.454962889817114 26.35748856391535 27.21166449167946 22.97588405458808 42-43 26.168930253600127 26.18652475107124 27.838528859696325 19.80601613563231 44-45 24.375151120830303 27.35527296331072 28.64792336143068 19.621652554428287 46-47 24.725852986059152 28.13324837056685 27.676980386150813 19.463918257223188 48-49 22.30721947046015 28.56926935615252 28.712623540830247 20.41088763255709 50-51 21.001962999032234 27.251622068137355 30.004379173900126 21.742035758930292 52-53 25.06940768832279 26.519338755000994 27.628069187143645 20.783184369532567 54-55 26.411152902002893 26.385810771320834 27.583278884618274 19.619757442058003 56-57 27.18205798962867 26.15239095061628 27.014793233701056 19.650757826054 58-59 26.114200836413975 26.387113241009285 27.034994170196697 20.463691752380043 60-61 23.509537147328015 26.48292288991616 26.75652535638954 23.251014606366283 62-63 24.919952189094968 26.456860627551965 27.03772252195376 21.58546466139931 64-65 23.734290724014787 27.205670757309946 28.191161675014285 20.86887684366098 66-67 22.167917395819074 27.432067274343456 27.746395676833295 22.653619653004174 68-69 21.604512617863218 26.79691904172452 30.88832822630043 20.710240114111826 70-71 21.179319259958433 29.241892738803386 29.34432472655577 20.23446327468241 72-73 20.704774571400907 32.42434950600652 26.710917945773932 20.159957976818635 74-75 20.8576129462978 32.81495108946415 26.18032985904976 20.14710610518829 76-77 20.722408295748302 32.6288639506846 26.21249902680269 20.43622872676441 78-79 20.699429910672123 32.35572148335873 26.59160460337197 20.353244002597183 80-81 20.522882258324625 32.180216632208285 26.705604065008725 20.591297044458365 82-83 20.193872447750955 32.15361418503922 26.841241038397158 20.811272328812674 84-85 20.332278149185143 31.789646714128345 26.82662709281261 21.0514480438739 86-87 20.19502545157261 31.642322803232524 27.35753782750585 20.80511391768901 88-89 20.31137239095398 31.636888175121136 27.38750197577363 20.664237458151256 90-91 20.6397621422828 31.18246800259859 27.093111210779856 21.08465864433875 92-93 20.444421146936325 31.47827454418955 26.844914842312935 21.23238946656119 94-95 20.41455254033014 31.42521508502426 26.794038261417857 21.366194113227742 96-97 20.65485697552896 31.622767166920916 26.634802322615382 21.08757353493474 98-99 20.88487268923116 31.258219167831037 26.630566119359262 21.226342023578542 100-101 20.940068155089005 31.357260923103265 26.202282786516818 21.500388135290912 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3189.0 1 2212.0 2 1005.5 3 988.5 4 1149.5 5 1087.5 6 1242.5 7 1519.0 8 1791.5 9 2041.0 10 2252.0 11 2373.5 12 2276.5 13 2102.5 14 1986.5 15 1881.0 16 1800.0 17 1776.5 18 1777.0 19 1875.5 20 2089.5 21 2394.5 22 2871.5 23 3722.5 24 4948.0 25 6885.0 26 10181.5 27 14973.0 28 20196.0 29 25292.5 30 30901.0 31 36348.5 32 47024.5 33 67268.0 34 96701.5 35 129347.0 36 168720.0 37 218889.0 38 258005.5 39 267827.0 40 267431.5 41 265682.0 42 265020.5 43 271835.5 44 269982.0 45 259318.0 46 247777.5 47 226407.5 48 199267.0 49 180378.0 50 165268.0 51 154232.5 52 144901.5 53 130322.5 54 113190.5 55 97370.5 56 84253.0 57 73588.0 58 64552.5 59 55981.5 60 47285.5 61 39515.0 62 32939.5 63 27715.5 64 23294.5 65 19098.5 66 15533.0 67 13046.0 68 11558.5 69 9539.5 70 7336.5 71 5931.0 72 4710.0 73 3735.5 74 3000.5 75 2391.0 76 1958.0 77 1547.0 78 1199.5 79 939.0 80 717.5 81 535.5 82 380.0 83 292.0 84 237.0 85 180.5 86 143.0 87 127.0 88 103.5 89 76.0 90 62.5 91 49.5 92 42.5 93 31.0 94 19.0 95 17.5 96 13.0 97 8.5 98 8.0 99 6.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.296574830596548 2 0.27357716685508277 3 0.2451436916838167 4 0.23250447979284616 5 0.23814936089302396 6 0.18848581101166154 7 0.15501565741767787 8 0.1524498023721425 9 0.13030742364585574 10-11 0.11510235670934982 12-13 0.13777691277841428 14-15 0.14039028365812625 16-17 0.1340231618784644 18-19 0.12063319980750385 20-21 0.12675323924944748 22-23 0.15722039212347122 24-25 0.5645356258519589 26-27 0.2217373917684329 28-29 0.1955466639703015 30-31 0.29418953572088363 32-33 0.3257590559478041 34-35 0.334292899765918 36-37 0.32512234376983784 38-39 0.33347562741808084 40-41 0.3363170743018403 42-43 0.34757832700169006 44-45 0.3279732938204327 46-47 0.3482530518469975 48-49 0.34395762043743455 50-51 0.34983057754166 52-53 0.33391277309250533 54-55 0.3263862649589349 56-57 0.33492010877704886 58-59 0.32748863231183156 60-61 0.30448146540353105 62-63 0.2911865350009237 64-65 0.30213418319520796 66-67 0.31797596230968006 68-69 0.304538484404543 70-71 0.2850189797248035 72-73 0.28083758631726435 74-75 0.27321604651534076 76-77 0.27603848706542966 78-79 0.2840591598744366 80-81 0.2938759312153182 82-83 0.31677856328843024 84-85 0.30771254212753857 86-87 0.3106585238464866 88-89 0.3159422846069224 90-91 0.30888143164828247 92-93 0.28992261381182666 94-95 0.2860168122425117 96-97 0.3029229460425392 98-99 0.31215052103963126 100-101 0.3127397173834208 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5261404.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.54439389595512 #Duplication Level Percentage of deduplicated Percentage of total 1 92.2412235350981 56.769301946889236 2 3.9668382756014706 4.882733147103365 3 1.1820177496099218 2.182396979220105 4 0.612380313015404 1.5075430079339325 5 0.4285292117505771 1.318678530195034 6 0.21572520108354035 0.7966006049261721 7 0.1878183328494451 0.80914158204375 8 0.13302997065544822 0.6549799131189005 9 0.11404066548410006 0.6316707273039277 >10 0.7164119828901764 8.985123098366136 >50 0.10308520603497609 4.3859333846771325 >100 0.08605798014649844 10.749723261342146 >500 0.010752192418025015 4.409239029544141 >1k 0.002089383362286648 1.9169347873362015 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0030600197209718167 0.0 0.0 2 0.003364121059701935 0.0 0.0 3 0.0038962984024796424 0.0 0.0 4 0.004238412408551026 0.0 0.0 5 0.004694564416646203 0.0 0.0 6 0.005416805096130234 0.0 0.0 7 0.006690229452062605 0.0 0.0 8 0.007583527134582328 0.0 0.0 9 0.008400799482419521 0.0 0.0 10-11 0.01207852504768689 0.0 0.0 12-13 0.01572774111244831 0.0 0.0 14-15 0.01990913451998744 0.0 0.0 16-17 0.02427108809739758 0.0 0.0 18-19 0.02954534569099807 0.0 1.90063336706324E-5 20-21 0.034477489278527174 0.0 1.90063336706324E-5 22-23 0.04015087987921095 0.0 1.90063336706324E-5 24-25 0.045947811648753834 0.0 1.90063336706324E-5 26-27 0.05203934159019152 0.0 1.90063336706324E-5 28-29 0.05874857737592475 0.0 1.90063336706324E-5 30-31 0.08524340651278632 0.0 1.90063336706324E-5 32-33 0.09382476616507684 0.0 1.90063336706324E-5 34-35 0.10137978379915323 0.0 3.80126673412648E-5 36-37 0.10999915611878502 0.0 3.80126673412648E-5 38-39 0.1203100921351031 0.0 3.80126673412648E-5 40-41 0.1305830154840799 0.0 3.80126673412648E-5 42-43 0.14184426818392962 0.0 3.80126673412648E-5 44-45 0.15401782489996968 0.0 3.80126673412648E-5 46-47 0.16732225846941234 0.0 3.80126673412648E-5 48-49 0.1817290593917517 0.0 3.80126673412648E-5 50-51 0.196915119994587 0.0 3.80126673412648E-5 52-53 0.21399231079765021 0.0 3.80126673412648E-5 54-55 0.2328656001325882 0.0 3.80126673412648E-5 56-57 0.2515203166303139 0.0 3.80126673412648E-5 58-59 0.27282641667509283 0.0 4.7515834176581E-5 60-61 0.2959381184185818 0.0 5.70190010118972E-5 62-63 0.3195249785038366 0.0 7.60253346825296E-5 64-65 0.34530707012804945 0.0 7.60253346825296E-5 66-67 0.374595830314494 0.0 7.60253346825296E-5 68-69 0.4050249705211765 0.0 7.60253346825296E-5 70-71 0.4375258010979579 0.0 7.60253346825296E-5 72-73 0.47344777173545316 0.0 7.60253346825296E-5 74-75 0.5122492019240492 0.0 9.5031668353162E-5 76-77 0.5549754400156308 0.0 1.140380020237944E-4 78-79 0.6027478596967654 0.0 1.140380020237944E-4 80-81 0.654644653784427 0.0 1.140380020237944E-4 82-83 0.7118632212998659 0.0 1.140380020237944E-4 84-85 0.7751543124230718 0.0 1.140380020237944E-4 86-87 0.8472548392026159 0.0 1.140380020237944E-4 88-89 0.9254373927567623 0.0 1.140380020237944E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4620 0.0 76.24443 9 ATTCGGA 1080 0.0 71.63983 2 ATCCGGA 1425 0.0 70.9505 2 ATCGGGA 7530 0.0 69.466805 2 CGGGAGA 6920 0.0 68.56893 4 TCGGGAG 7065 0.0 67.83843 3 CGGAAAG 2975 0.0 65.55236 5 TCGGAGA 1710 0.0 65.48614 4 GTTCGGA 1250 0.0 65.31443 2 AGAGCGG 5585 0.0 65.28137 8 GATCGGG 8415 0.0 64.314316 1 AAGCGGC 1375 0.0 62.40755 9 GATCCGG 1720 0.0 62.102768 1 TCGGGAA 2045 0.0 58.939568 3 CGGAGAG 3775 0.0 57.945194 5 TCGGAAA 2070 0.0 57.535625 4 ATCGGAG 1395 0.0 57.488247 3 GAGAGCG 6680 0.0 57.277924 7 GGATCGG 2825 0.0 56.968945 1 ACCGGAA 1025 0.0 56.01806 3 >>END_MODULE