##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_34211-2.34200000035860.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3569610 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.948689632761003 33.0 33.0 33.0 33.0 33.0 2 31.83552292827508 33.0 33.0 33.0 33.0 33.0 3 31.78958401618104 33.0 33.0 33.0 33.0 33.0 4 35.48430864996456 37.0 37.0 37.0 33.0 37.0 5 35.52286524298172 37.0 37.0 37.0 33.0 37.0 6 35.62565042119447 37.0 37.0 37.0 33.0 37.0 7 35.50067038135819 37.0 37.0 37.0 33.0 37.0 8 35.47487120441729 37.0 37.0 37.0 33.0 37.0 9 35.328866458800825 37.0 37.0 37.0 33.0 37.0 10-11 35.308293342970245 37.0 37.0 37.0 33.0 37.0 12-13 35.28733475085514 37.0 37.0 37.0 33.0 37.0 14-15 37.0231012631632 40.0 37.0 40.0 33.0 40.0 16-17 36.993987298332314 40.0 37.0 40.0 33.0 40.0 18-19 36.957549003952806 40.0 37.0 40.0 33.0 40.0 20-21 36.9402294648435 40.0 37.0 40.0 33.0 40.0 22-23 37.066929860685065 40.0 37.0 40.0 33.0 40.0 24-25 36.72337608310151 40.0 37.0 40.0 33.0 40.0 26-27 36.64462070086088 40.0 37.0 40.0 33.0 40.0 28-29 36.566459501178 40.0 37.0 40.0 33.0 40.0 30-31 36.53804561282605 40.0 37.0 40.0 33.0 40.0 32-33 36.39432907236365 40.0 37.0 40.0 33.0 40.0 34-35 36.37922896338816 40.0 37.0 40.0 33.0 40.0 36-37 36.24900115138628 40.0 37.0 40.0 33.0 40.0 38-39 36.15777984149529 40.0 37.0 40.0 33.0 40.0 40-41 36.20535660758458 40.0 37.0 40.0 33.0 40.0 42-43 36.05352545516177 37.0 37.0 40.0 27.0 40.0 44-45 35.851619924865744 37.0 37.0 40.0 27.0 40.0 46-47 35.71640263222032 37.0 37.0 40.0 27.0 40.0 48-49 35.58946103355829 37.0 37.0 40.0 27.0 40.0 50-51 34.514103221360315 37.0 35.0 38.5 24.5 38.5 52-53 34.92642053333557 37.0 35.0 38.5 27.0 40.0 54-55 35.62571219264849 37.0 37.0 40.0 27.0 40.0 56-57 35.73078879765577 37.0 37.0 40.0 27.0 40.0 58-59 35.51667339009023 37.0 37.0 40.0 27.0 40.0 60-61 35.3143680962346 37.0 37.0 40.0 27.0 40.0 62-63 35.049962460885084 37.0 35.0 40.0 27.0 40.0 64-65 34.857807295474856 37.0 33.0 40.0 27.0 40.0 66-67 34.618554407904504 37.0 33.0 40.0 27.0 40.0 68-69 34.458379766977345 37.0 33.0 40.0 27.0 40.0 70-71 34.15342866587666 37.0 33.0 38.5 27.0 40.0 72-73 34.0049362535403 37.0 33.0 37.0 27.0 40.0 74-75 33.8214423984693 37.0 33.0 37.0 27.0 40.0 76-77 33.53262905471466 37.0 33.0 37.0 24.5 40.0 78-79 33.177491658752636 37.0 33.0 37.0 22.0 37.0 80-81 32.790962878297634 37.0 33.0 37.0 22.0 37.0 82-83 32.4770312443096 37.0 33.0 37.0 22.0 37.0 84-85 32.15662817506674 37.0 33.0 37.0 22.0 37.0 86-87 31.974393561201364 37.0 33.0 37.0 22.0 37.0 88-89 31.79389611190018 33.0 33.0 37.0 18.5 37.0 90-91 31.610654105070303 33.0 33.0 37.0 15.0 37.0 92-93 31.412719176604725 33.0 33.0 37.0 15.0 37.0 94-95 31.23608587492751 33.0 33.0 37.0 15.0 37.0 96-97 31.065169864495 33.0 33.0 37.0 15.0 37.0 98-99 30.887974316521973 33.0 30.0 37.0 15.0 37.0 100-101 29.500806530685423 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1509356238869337 2306 2 0.2166667257691124 2306 3 0.16209726488293796 2306 4 0.28981788763275773 2306 5 0.2611816846079549 2306 6 0.19652116521076124 2306 7 0.3275728876041626 2306 8 0.25486658360077286 2306 9 0.37590796824643036 2306 10-11 0.2754009420694459 2306 12-13 0.33740581044295936 2306 14-15 0.5327830807384615 2306 16-17 0.5240260849798588 2306 18-19 0.4856666845877342 2306 20-21 0.4073360732885263 2306 22-23 0.3439563266049461 2306 24-25 0.37328367890243186 2306 26-27 0.26030126672062437 2306 28-29 0.17231608313750257 2306 30-31 0.08531158041272136 2306 32-33 0.20749773344866895 2306 34-35 0.21980821761517433 2306 36-37 0.1268688900833581 2306 38-39 0.15616849374602282 2306 40-41 0.03775825060852611 2306 42-43 0.20865096189523769 2306 44-45 0.31392459574757936 2306 46-47 0.2839494534064002 2306 48-49 0.2310944263988759 2306 50-51 0.0612618588294751 2306 52-53 0.1085425142102423 2306 54-55 0.18869712006435435 2306 56-57 0.14964261503182286 2306 58-59 0.22844662847642638 2306 60-61 0.16550822719971592 2306 62-63 0.19227831963131337 2306 64-65 0.07668957831972278 2306 66-67 0.11874466424656305 2306 68-69 -0.0790684271010349 2306 70-71 0.04525617118378733 2306 72-73 0.07375387833651104 2306 74-75 0.029730602285994223 2306 76-77 0.022497022172991876 2306 78-79 0.07074478015422159 2306 80-81 0.08709236894360117 2306 82-83 0.18936304378132007 2306 84-85 0.25058889810829754 2306 86-87 0.22662380852170116 2306 88-89 0.27563348930371845 2306 90-91 -0.00624092204311566 2306 92-93 0.15236378945788331 2306 94-95 0.044943060871052154 2306 96-97 -0.01621821628640774 2306 98-99 0.14079510894095293 2306 100-101 -0.13749839555114107 2307 1 0.13152426511228654 2307 2 0.08072325459313134 2307 3 0.10958374890783773 2307 4 0.2204474801562526 2307 5 0.16473651653707577 2307 6 0.2261031481405027 2307 7 0.2916576951671743 2307 8 0.1582981011252329 2307 9 0.297153215183954 2307 10-11 0.23801322554862736 2307 12-13 0.23685655819217288 2307 14-15 0.347281290035923 2307 16-17 0.4162205560599901 2307 18-19 0.37005013473715564 2307 20-21 0.26690510053060024 2307 22-23 0.28308301501451894 2307 24-25 0.49186386788900194 2307 26-27 0.38027321994233887 2307 28-29 0.3342776997808272 2307 30-31 0.19798009730713062 2307 32-33 0.24509216789265054 2307 34-35 0.062415800478866856 2307 36-37 0.2719682426059862 2307 38-39 0.219746108992112 2307 40-41 0.1930211490735232 2307 42-43 0.1891331740908484 2307 44-45 0.3652208296949624 2307 46-47 0.4013107400515068 2307 48-49 0.17041640525565782 2307 50-51 0.005640410514587302 2307 52-53 0.11268275697077001 2307 54-55 0.20215570194528 2307 56-57 0.043486565513667585 2307 58-59 0.08263425262231294 2307 60-61 0.20521810916924466 2307 62-63 0.31876393414469106 2307 64-65 0.32780810648811354 2307 66-67 0.3463584094193095 2307 68-69 0.3042886822046569 2307 70-71 0.3341663003961415 2307 72-73 0.1738220679616589 2307 74-75 0.2992043005003424 2307 76-77 0.19420354685241392 2307 78-79 0.3408344534341623 2307 80-81 0.4578746496017274 2307 82-83 0.3807628618286216 2307 84-85 0.4757842823631506 2307 86-87 0.40567478905030896 2307 88-89 0.23564303721571278 2307 90-91 0.4328792851114436 2307 92-93 0.4672509593360097 2307 94-95 0.5124544909053945 2307 96-97 0.37247194254186056 2307 98-99 0.29837539719306605 2307 100-101 0.25929196757311246 2201 1 0.17621049072021222 2201 2 0.1307377366853224 2201 3 0.11184096880751682 2201 4 0.1774818039963364 2201 5 0.10193678208482027 2201 6 0.21498141398045334 2201 7 0.31436574387826965 2201 8 0.16440525066500555 2201 9 0.2532407562272283 2201 10-11 0.24445038736614322 2201 12-13 0.29399257707784443 2201 14-15 0.5461156097748869 2201 16-17 0.41708067058386433 2201 18-19 0.4170514124934357 2201 20-21 0.32606252471749286 2201 22-23 0.42055972191491975 2201 24-25 0.49485945021008604 2201 26-27 0.34595610826105627 2201 28-29 0.3096474096519728 2201 30-31 0.2643109631311873 2201 32-33 0.2480755750034902 2201 34-35 0.28067534776609904 2201 36-37 0.36147494153482285 2201 38-39 0.2257592210527477 2201 40-41 0.3273656229378332 2201 42-43 0.38819978402531063 2201 44-45 0.4551978762196427 2201 46-47 0.375499774928997 2201 48-49 0.3636326664664651 2201 50-51 0.3059498210549876 2201 52-53 0.44076446288433146 2201 54-55 0.49919430633297424 2201 56-57 0.5003763888321444 2201 58-59 0.4567521906799712 2201 60-61 0.41479016334508145 2201 62-63 0.359506032433238 2201 64-65 0.30232747404361504 2201 66-67 0.3928689941626118 2201 68-69 0.43811042302903047 2201 70-71 0.4457623551153773 2201 72-73 0.32001013779353826 2201 74-75 0.4656237249589239 2201 76-77 0.47152135444308385 2201 78-79 0.5390337398386791 2201 80-81 0.45691184679143504 2201 82-83 0.4045117308156634 2201 84-85 0.4769285919262032 2201 86-87 0.34254408111950596 2201 88-89 0.47195746009792927 2201 90-91 0.31953913152436897 2201 92-93 0.46549692805981735 2201 94-95 0.49099664404647214 2201 96-97 0.44826022332528126 2201 98-99 0.41982327960360166 2201 100-101 0.30488877074737886 1101 1 0.25468399244446616 1101 2 0.24246665055685312 1101 3 0.23460107612570624 1101 4 0.3890384094263979 1101 5 0.2945734055885296 1101 6 0.24336899559870062 1101 7 0.397603480363415 1101 8 0.4437752362201266 1101 9 0.4838914664142635 1101 10-11 0.44741059254429416 1101 12-13 0.40675305897960357 1101 14-15 0.576146969443009 1101 16-17 0.5192552159915849 1101 18-19 0.5779205692824121 1101 20-21 0.5623416456635013 1101 22-23 0.5442290723167744 1101 24-25 0.49054486205301373 1101 26-27 0.49641901161147217 1101 28-29 0.4338989741236503 1101 30-31 0.3879209069202503 1101 32-33 0.3799660104802953 1101 34-35 0.4405303488079184 1101 36-37 0.4574160359275439 1101 38-39 0.41376286036623355 1101 40-41 0.3205364962560111 1101 42-43 0.4538835980351976 1101 44-45 0.5093623675702759 1101 46-47 0.447991791616694 1101 48-49 0.373691844817408 1101 50-51 0.34848071249364665 1101 52-53 0.3065100539186716 1101 54-55 0.570766691095848 1101 56-57 0.49399178749441575 1101 58-59 0.6206968834160875 1101 60-61 0.5348680474629717 1101 62-63 0.5422337499785002 1101 64-65 0.6665776572383848 1101 66-67 0.5918501268565066 1101 68-69 0.44180694829820766 1101 70-71 0.5813586804505704 1101 72-73 0.2251687973462353 1101 74-75 0.23573096121112513 1101 76-77 0.37944066563072454 1101 78-79 0.43076943133164747 1101 80-81 0.5237325804601696 1101 82-83 0.431007519091672 1101 84-85 0.41909069413600974 1101 86-87 0.45723600954594446 1101 88-89 0.4159531196108297 1101 90-91 0.3911675575007969 1101 92-93 0.41466842529375114 1101 94-95 0.5763841390422648 1101 96-97 0.28991072632077675 1101 98-99 0.4706523676564913 1101 100-101 0.3046060772105861 2304 1 0.11743474361246697 2304 2 0.06972753597985104 2304 3 0.09894274033189987 2304 4 0.1655168643650029 2304 5 0.195976743727023 2304 6 0.14344020458840134 2304 7 0.23163095661261224 2304 8 0.19499881507974237 2304 9 0.18782880250332568 2304 10-11 0.1557394391323399 2304 12-13 0.22715790813812475 2304 14-15 0.22103121141462623 2304 16-17 0.1606363230308503 2304 18-19 0.3121269364081769 2304 20-21 0.1971307107415754 2304 22-23 0.08974030075868455 2304 24-25 0.10041859635663997 2304 26-27 0.12999318185305953 2304 28-29 0.044547323290856866 2304 30-31 0.08624402205303028 2304 32-33 0.060686610719976386 2304 34-35 0.06514955439347858 2304 36-37 0.046425536248847266 2304 38-39 0.1344812027598934 2304 40-41 0.052965243846195165 2304 42-43 0.19743397405340346 2304 44-45 -0.053212097928096114 2304 46-47 -0.02048520440602175 2304 48-49 0.040181067944701 2304 50-51 0.10237810207629394 2304 52-53 0.09140593248129392 2304 54-55 0.2830458998076679 2304 56-57 0.15058990827180452 2304 58-59 0.06832093468656097 2304 60-61 0.14023998417381023 2304 62-63 0.22547358532942496 2304 64-65 0.21621640187600377 2304 66-67 0.3152822264796171 2304 68-69 0.24977106591461506 2304 70-71 0.17672141209688874 2304 72-73 0.15675693149702852 2304 74-75 0.19030744140287226 2304 76-77 0.20676320761797484 2304 78-79 0.23688298361815185 2304 80-81 0.23372094386917297 2304 82-83 0.13761432389219408 2304 84-85 0.3698105996538388 2304 86-87 0.26853218683896785 2304 88-89 0.13294775288453664 2304 90-91 0.039259677618208144 2304 92-93 0.12157396049009961 2304 94-95 0.22336383579617802 2304 96-97 0.0523844571507972 2304 98-99 0.10393388631845824 2304 100-101 0.12941112089121987 2202 1 0.1307704533263454 2202 2 0.034262826134106916 2202 3 0.053536370227089236 2202 4 0.07152864551239446 2202 5 0.020464261406871742 2202 6 0.13239256397902466 2202 7 0.06784398548021642 2202 8 0.11364748146662151 2202 9 0.12385246354635626 2202 10-11 0.16586932318142544 2202 12-13 0.17844708454764913 2202 14-15 0.15845191928298874 2202 16-17 0.07356349198489198 2202 18-19 0.12191505518785561 2202 20-21 0.14953345425685427 2202 22-23 0.16789815223771143 2202 24-25 0.12251989880987679 2202 26-27 0.19399432829610674 2202 28-29 0.129495742385636 2202 30-31 0.22738254115449053 2202 32-33 -0.005807507437118886 2202 34-35 0.030765957199612615 2202 36-37 0.16258881767713973 2202 38-39 0.07228088712678016 2202 40-41 0.10716201417643845 2202 42-43 0.2654032946594498 2202 44-45 0.17842852589588176 2202 46-47 0.1901324598487335 2202 48-49 0.1593142630706339 2202 50-51 0.158867035950486 2202 52-53 0.06427732071905723 2202 54-55 0.2947419671587852 2202 56-57 0.38463174019627644 2202 58-59 0.14322376701505135 2202 60-61 0.2344936792548893 2202 62-63 0.3692496459849295 2202 64-65 0.36501700207265486 2202 66-67 0.27549535409546877 2202 68-69 0.23411146075104483 2202 70-71 0.35877650759668 2202 72-73 0.19314018066354066 2202 74-75 0.250814345049271 2202 76-77 0.20944807607507698 2202 78-79 0.15515243265712542 2202 80-81 0.21999824941490687 2202 82-83 0.20900262186382434 2202 84-85 0.21985795666306984 2202 86-87 0.2926243502624928 2202 88-89 0.19990309760321523 2202 90-91 0.15261459507009434 2202 92-93 0.12286671953414441 2202 94-95 0.13792293993282811 2202 96-97 0.15168400694309625 2202 98-99 0.009727819202165477 2202 100-101 0.1920701341674409 1102 1 0.16466544713285103 1102 2 0.16025028480414605 1102 3 0.16956147164550828 1102 4 0.1530903495422109 1102 5 0.15089737577035578 1102 6 0.23031987866485792 1102 7 0.20946174329061762 1102 8 0.31269734260050086 1102 9 0.20507800689776445 1102 10-11 0.2625943358593972 1102 12-13 0.2386415354876945 1102 14-15 0.2816221404349406 1102 16-17 0.3195802437433386 1102 18-19 0.2721898031259684 1102 20-21 0.2451916310722524 1102 22-23 0.2535965830686564 1102 24-25 0.2894400893193989 1102 26-27 0.36465246780769434 1102 28-29 0.4211226449602208 1102 30-31 0.40927568426536 1102 32-33 0.38437282773873704 1102 34-35 0.30128454846254726 1102 36-37 0.33198001299587077 1102 38-39 0.382931457161078 1102 40-41 0.29640678029110035 1102 42-43 0.32345155045022267 1102 44-45 0.3647292748073028 1102 46-47 0.291125935091479 1102 48-49 0.4004897255153921 1102 50-51 0.3130997721172193 1102 52-53 0.40940247928692486 1102 54-55 0.5455581213740359 1102 56-57 0.5316590843201467 1102 58-59 0.6115583157422506 1102 60-61 0.46656232083534377 1102 62-63 0.6308475624282153 1102 64-65 0.6081744695826288 1102 66-67 0.6130809850063912 1102 68-69 0.5328257154346403 1102 70-71 0.40967596656343375 1102 72-73 0.43687138087226884 1102 74-75 0.354855382231797 1102 76-77 0.32745337065905034 1102 78-79 0.42095699391202146 1102 80-81 0.45902346658174764 1102 82-83 0.4250118638310809 1102 84-85 0.47331149070940626 1102 86-87 0.5898999033235484 1102 88-89 0.40527206538956406 1102 90-91 0.39840027633050923 1102 92-93 0.4213235245922853 1102 94-95 0.40086182199926057 1102 96-97 0.2880087795123849 1102 98-99 0.3971921700763197 1102 100-101 0.3779916631379052 2305 1 0.11977746014925472 2305 2 0.0384086098734322 2305 3 0.10498527660699608 2305 4 0.05200957507583581 2305 5 0.149936674912297 2305 6 0.28028966894387963 2305 7 0.14416908230229808 2305 8 0.25969836750984854 2305 9 0.09268627239688243 2305 10-11 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0.07786757608662498 1213 76-77 0.04290992503764102 1213 78-79 0.13674085728263918 1213 80-81 0.05654051515875835 1213 82-83 0.01191366795898574 1213 84-85 0.05801904563462301 1213 86-87 0.09936597972906469 1213 88-89 -0.007316593132564719 1213 90-91 -0.04267189550063577 1213 92-93 0.0379592887481337 1213 94-95 0.15211819037672925 1213 96-97 0.13912815001443946 1213 98-99 0.02009683393795214 1213 100-101 0.16604833109024142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1793.0 3 355.0 4 375.0 5 406.0 6 1003.0 7 2628.0 8 9846.0 9 20986.0 10 27046.0 11 18754.0 12 11516.0 13 8802.0 14 7943.0 15 7597.0 16 8000.0 17 8689.0 18 9745.0 19 11256.0 20 12626.0 21 14522.0 22 16440.0 23 18665.0 24 21279.0 25 25533.0 26 30705.0 27 38002.0 28 46318.0 29 54766.0 30 64081.0 31 79814.0 32 104735.0 33 145884.0 34 214188.0 35 337645.0 36 582087.0 37 1018257.0 38 583742.0 39 3581.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.39209945504279 29.11279729249409 16.214184060387833 18.28091919207529 2 32.25640259249937 26.281529790370715 27.152335182122183 14.30973243500773 3 35.339340186058166 23.60530798787471 23.211971842456602 17.84337998361053 4 30.365238399972146 23.832499874342332 27.189707696398724 18.6125540292868 5 33.07866911502265 21.51161925387297 27.083239142393957 18.326472488710426 6 31.325232896221088 21.89761968849122 28.489106903933447 18.288040511354243 7 34.1077075755905 22.457975347632278 23.159005835794726 20.27531124098249 8 29.551671129164987 28.0926965133593 22.786546121694755 19.56908623578096 9 29.64513593528831 24.36559885414791 25.193317658094657 20.79594755246913 10-11 25.9175972921547 29.699911144618863 27.929142321189765 16.453349242036673 12-13 26.49441589775765 28.227766231460024 27.416335250643932 17.861482620138396 14-15 25.17151214454249 28.2057015154956 28.08668206742322 18.536104272538687 16-17 25.524601858587037 28.028731880571744 28.185180395489052 18.261485865352167 18-19 24.991462528177163 28.107334708585057 28.51925106983112 18.38195169340666 20-21 24.45080572150276 28.875496234543185 28.114859231031936 18.55883881292212 22-23 25.279353337726462 28.676880850068624 27.506070946705723 18.53769486549919 24-25 23.621345308106893 29.775171522684158 27.84139078827884 18.76209238093011 26-27 23.131528263926647 30.084214016782717 27.84359359559611 18.94066412369452 28-29 23.432898977770886 29.809848683144065 27.783032273865054 18.97422006522 30-31 23.992807458461584 28.619490303991512 28.29141734906319 19.096284888483712 32-33 23.07967325930644 29.34932371242277 28.411203421434443 19.159799606836348 34-35 24.092222446786472 28.678538088610928 28.036993321446737 19.192246143155867 36-37 23.080581315260137 28.942401242780534 28.558121899355182 19.418895542604147 38-39 22.7397788576787 29.839610538553735 28.05441025341192 19.36620035035564 40-41 24.008466223022488 28.483732851562383 27.984032295219823 19.523768630195303 42-43 23.790497154937814 28.443987135370985 28.16667791197175 19.59883779771945 44-45 22.826891693542358 28.961969656000523 28.485430911438677 19.725707739018443 46-47 23.173107983079053 29.071220190210262 27.907420022035357 19.84825180467533 48-49 22.00276936782875 29.092418479563364 28.583145482895688 20.321666669712194 50-51 21.570282321321134 28.46754513711224 29.26057228966471 20.701600251901915 52-53 23.252150535518624 28.424496962180594 28.132155685222653 20.19119681707813 54-55 23.24765652448879 28.38823694750896 28.505459911460896 19.858646616541353 56-57 23.48173699210977 28.281836052191505 28.301878571567112 19.93454838413161 58-59 23.054421636772528 28.566686370291546 28.037077668541183 20.341814324394747 60-61 22.036927311167542 28.359296524423065 28.04810074043183 21.55567542397756 62-63 22.712908581529177 28.461632047419382 28.1778204358409 20.647638935210537 64-65 21.933498704917103 28.937076604135974 28.790560471976402 20.338864218970524 66-67 21.57843089999737 28.79202961491921 28.308944887701102 21.320594597382318 68-69 21.311714960301558 28.497576841086524 29.7912115457354 20.399496652876515 70-71 21.221245534349006 29.518299001910936 28.939876825225753 20.320578638514306 72-73 21.182867763476455 30.56887519987661 27.967989400411614 20.28026763623532 74-75 20.98042382963661 30.78556108194967 28.058732724946168 20.175282363467552 76-77 21.1455986982829 30.69356552382483 27.886091887697685 20.27474389019459 78-79 21.077869597454086 30.82851797460772 27.911979728433156 20.18163269950504 80-81 21.123959046766064 30.659206574996052 27.909411165551518 20.30742321268637 82-83 21.249859827937563 30.639280160601352 27.738849681307048 20.37201033015404 84-85 21.1712850364762 30.42228193355106 27.88993362637566 20.516499403597077 86-87 21.444438589650364 30.07515447874183 27.894768273105065 20.58563865850274 88-89 21.47252178060284 30.212502014738256 27.760528667318972 20.554447537339932 90-91 21.513934594383446 29.962651858554807 27.81755053521095 20.7058630118508 92-93 21.60472077849463 29.903115248516887 27.72676263544956 20.765401337538922 94-95 21.52369449070921 29.82301760158956 27.886233066533606 20.767054841167624 96-97 21.648436831503044 29.665573958446178 27.81054122707888 20.875447982971906 98-99 21.62482198298484 29.755915574793846 27.782710692402755 20.836551749818558 100-101 21.85792979366734 29.578802472990485 27.710710474686806 20.85255725865537 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1041.0 1 808.5 2 414.5 3 244.0 4 253.5 5 306.0 6 424.0 7 564.5 8 727.0 9 923.5 10 1082.0 11 1136.5 12 1156.0 13 1126.5 14 989.5 15 856.5 16 798.5 17 845.0 18 1017.5 19 1380.0 20 1997.0 21 2944.5 22 4324.0 23 6269.5 24 8971.5 25 12388.0 26 16660.5 27 21863.0 28 27930.0 29 34928.0 30 42697.0 31 51488.0 32 61878.0 33 73202.5 34 85301.0 35 98640.0 36 112181.0 37 125587.0 38 138495.0 39 148866.0 40 157659.0 41 164624.5 42 168128.5 43 169189.5 44 168346.0 45 165894.0 46 162120.5 47 157773.0 48 151476.5 49 142282.5 50 132335.0 51 121352.5 52 107904.0 53 92844.0 54 78267.5 55 65193.5 56 53703.0 57 44124.0 58 36140.0 59 29308.0 60 23620.5 61 18842.5 62 14656.5 63 11341.0 64 8965.5 65 7024.0 66 5362.5 67 4126.0 68 3152.0 69 2478.5 70 1987.5 71 1552.0 72 1207.0 73 957.5 74 736.0 75 560.0 76 452.0 77 356.5 78 267.5 79 210.0 80 180.5 81 143.0 82 114.5 83 96.5 84 73.5 85 60.0 86 52.5 87 44.0 88 31.0 89 26.0 90 21.5 91 14.0 92 13.0 93 13.5 94 10.0 95 4.0 96 2.0 97 2.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29737142152784196 2 0.2745958241936794 3 0.24618935962191948 4 0.23383506881704164 5 0.24039040679514007 6 0.1892923876838086 7 0.15606746955549766 8 0.15304192895022145 9 0.13312378663215307 10-11 0.11950885390841015 12-13 0.13955306041836502 14-15 0.14193427293177685 16-17 0.13612131297256563 18-19 0.124509400186575 20-21 0.12991615330526304 22-23 0.15994744523911575 24-25 0.5706785895377926 26-27 0.22494053972282688 28-29 0.19757060295102266 30-31 0.2982258566061839 32-33 0.3306803824507439 34-35 0.33905664764498084 36-37 0.3300500614913114 38-39 0.33846834808284376 40-41 0.34119973890705146 42-43 0.35213930933631404 44-45 0.33258535246147336 46-47 0.35295172301736044 48-49 0.3496320326310157 50-51 0.3549407358226809 52-53 0.33964494720711785 54-55 0.3322491812831094 56-57 0.3410176461854376 58-59 0.33306159496415577 60-61 0.3103560332921524 62-63 0.2958026226954765 64-65 0.3069663072436485 66-67 0.3232425951294399 68-69 0.3090533699759918 70-71 0.290185762590311 72-73 0.28648787962830674 74-75 0.27804157877191066 76-77 0.28025470569614047 78-79 0.28963948442546944 80-81 0.29898224175750293 82-83 0.3216878034295063 84-85 0.3135776737514743 86-87 0.3157908006757041 88-89 0.3209174111457554 90-91 0.31375976647308806 92-93 0.29515829460361215 94-95 0.29095615487406185 96-97 0.3087312059300596 98-99 0.3169113712702508 100-101 0.317317578110774 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3569610.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.1291588170364 #Duplication Level Percentage of deduplicated Percentage of total 1 97.52094537573295 92.7708550063563 2 1.8465354003609542 3.5131871872443416 3 0.32776805303390005 0.9354089751663806 4 0.11812913569305217 0.4495010124105441 5 0.04204866543203937 0.20000270859644506 6 0.02630993479903783 0.15017051795781253 7 0.022576693504060944 0.15033913033379573 8 0.014832584303651369 0.11288090143113058 9 0.011732721698126027 0.10045115511994078 >10 0.06664563671969857 1.0427867778997508 >50 0.0023054856365369333 0.14776220305348986 >100 1.4091180332718406E-4 0.013941014170622957 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 2.9401282673032458E-5 0.41271341025938363 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 14756 0.4133784923282936 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0032216404593218865 0.0 0.0 2 0.004258168259277624 0.0 0.0 3 0.006891509156462471 0.0 0.0 4 0.009328750199601638 0.0 0.0 5 0.012382305069741513 0.0 2.801426486366858E-5 6 0.021823112328797824 0.0 2.801426486366858E-5 7 0.03137597664730881 0.0 5.602852972733716E-5 8 0.040480612728001096 2.801426486366858E-5 5.602852972733716E-5 9 0.044822823781869726 2.801426486366858E-5 8.404279459100574E-5 10-11 0.05493597339765409 2.801426486366858E-5 8.404279459100574E-5 12-13 0.06822874207546484 2.801426486366858E-5 9.804992702284004E-5 14-15 0.08059704001277451 2.801426486366858E-5 1.260641918865086E-4 16-17 0.09435204406083578 2.801426486366858E-5 1.400713243183429E-4 18-19 0.11177691680603763 2.801426486366858E-5 1.540784567501772E-4 20-21 0.13196119464031086 2.801426486366858E-5 1.6808558918201148E-4 22-23 0.15361622137992667 2.801426486366858E-5 1.6808558918201148E-4 24-25 0.17503312686820127 2.801426486366858E-5 1.8209272161384576E-4 26-27 0.19605783264838456 2.801426486366858E-5 2.1010698647751436E-4 28-29 0.21953378660413883 2.801426486366858E-5 2.521283837730172E-4 30-31 0.2508957561190158 2.801426486366858E-5 2.661355162048515E-4 32-33 0.28539532329862366 2.801426486366858E-5 2.941497810685201E-4 34-35 0.3254837363185334 2.801426486366858E-5 3.3617117836402295E-4 36-37 0.3740044430624074 2.801426486366858E-5 3.781925756595258E-4 38-39 0.4274836746871507 2.801426486366858E-5 3.921997080913601E-4 40-41 0.4903756993060867 2.801426486366858E-5 4.34221105386863E-4 42-43 0.5581982345410283 2.801426486366858E-5 4.902496351142002E-4 44-45 0.6313014587027714 2.801426486366858E-5 5.882995621370402E-4 46-47 0.7108199495182947 5.602852972733716E-5 5.882995621370402E-4 48-49 0.7948767512417323 5.602852972733716E-5 5.882995621370402E-4 50-51 0.8854748838108365 5.602852972733716E-5 5.882995621370402E-4 52-53 0.979000507058194 5.602852972733716E-5 6.163138270007088E-4 54-55 1.0786892685755587 5.602852972733716E-5 6.163138270007088E-4 56-57 1.1806331784144486 5.602852972733716E-5 6.443280918643773E-4 58-59 1.2858127358450924 5.602852972733716E-5 6.583352242962116E-4 60-61 1.3950683688133998 5.602852972733716E-5 6.863494891598802E-4 62-63 1.5085401486436893 5.602852972733716E-5 7.003566215917145E-4 64-65 1.6250654833441187 5.602852972733716E-5 7.423780188872173E-4 66-67 1.7480761203604875 5.602852972733716E-5 7.563851513190516E-4 68-69 1.87600326086043 5.602852972733716E-5 7.563851513190516E-4 70-71 2.003552208784713 5.602852972733716E-5 7.703922837508859E-4 72-73 2.1417465773571904 5.602852972733716E-5 7.984065486145545E-4 74-75 2.280487224094509 5.602852972733716E-5 8.824493432055603E-4 76-77 2.4273800219071555 5.602852972733716E-5 9.244707405010632E-4 78-79 2.5798336512952393 5.602852972733716E-5 9.524850053647318E-4 80-81 2.7355229282750777 5.602852972733716E-5 9.524850053647318E-4 82-83 2.900737055308563 5.602852972733716E-5 9.524850053647318E-4 84-85 3.072506520320147 5.602852972733716E-5 9.945064026602346E-4 86-87 3.254767327523175 5.602852972733716E-5 0.001008513535092069 88-89 3.4459086566879855 5.602852972733716E-5 0.001008513535092069 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 1415 0.0 73.12463 2 GAGCGGC 1095 0.0 65.363464 9 GATCGGG 1655 0.0 65.11347 1 TCGGGAG 1670 0.0 52.280155 3 GATCCGG 510 0.0 51.19593 1 AGAGCGG 1480 0.0 49.64259 8 TCGGGAA 725 0.0 43.850273 3 AAGCGGC 490 0.0 42.56267 9 CGGGAGA 1995 0.0 42.33449 4 CGGAAAG 1020 0.0 42.330994 5 GAGAGCG 2005 0.0 40.42991 7 AGCGGCG 1060 0.0 40.02114 10-11 GGATCGG 875 0.0 39.605705 1 TCCGGAG 510 0.0 39.07641 3 GTTCGGA 335 0.0 38.254505 2 CCGGAAA 485 0.0 38.154003 4 GCGTCGG 320 0.0 37.03073 10-11 TCGGAAA 810 0.0 36.903946 4 ATCCGGA 680 0.0 36.295944 2 GTCCGGA 230 0.0 35.08203 2 >>END_MODULE