FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_34211-2.34200000035860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_34211-2.34200000035860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569610
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT147560.4133784923282936Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA14150.073.124632
GAGCGGC10950.065.3634649
GATCGGG16550.065.113471
TCGGGAG16700.052.2801553
GATCCGG5100.051.195931
AGAGCGG14800.049.642598
TCGGGAA7250.043.8502733
AAGCGGC4900.042.562679
CGGGAGA19950.042.334494
CGGAAAG10200.042.3309945
GAGAGCG20050.040.429917
AGCGGCG10600.040.0211410-11
GGATCGG8750.039.6057051
TCCGGAG5100.039.076413
GTTCGGA3350.038.2545052
CCGGAAA4850.038.1540034
GCGTCGG3200.037.0307310-11
TCGGAAA8100.036.9039464
ATCCGGA6800.036.2959442
GTCCGGA2300.035.082032
Produced by FastQC (version 0.11.2)