##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_34211-1.34200000035897.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3899824 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.039526399140065 33.0 33.0 33.0 33.0 33.0 2 31.98011935923262 33.0 33.0 33.0 33.0 33.0 3 31.94526445295993 33.0 33.0 33.0 33.0 33.0 4 35.68154537230398 37.0 37.0 37.0 33.0 37.0 5 35.69629039669483 37.0 37.0 37.0 33.0 37.0 6 35.704488202544525 37.0 37.0 37.0 33.0 37.0 7 35.63705746720878 37.0 37.0 37.0 33.0 37.0 8 35.54945043673766 37.0 37.0 37.0 33.0 37.0 9 35.466323608450026 37.0 37.0 37.0 33.0 37.0 10-11 35.39980688872113 37.0 37.0 37.0 33.0 37.0 12-13 35.37812180754824 37.0 37.0 37.0 33.0 37.0 14-15 37.04790549522235 40.0 37.0 40.0 33.0 40.0 16-17 37.017692593306776 40.0 37.0 40.0 33.0 40.0 18-19 36.96059976039945 40.0 37.0 40.0 33.0 40.0 20-21 36.93565158325094 40.0 37.0 40.0 33.0 40.0 22-23 36.92979401121691 40.0 37.0 40.0 33.0 40.0 24-25 36.64377200099287 40.0 37.0 40.0 33.0 40.0 26-27 36.5734310061172 40.0 37.0 40.0 33.0 40.0 28-29 36.517922219053986 40.0 37.0 40.0 33.0 40.0 30-31 36.4773914156126 40.0 37.0 40.0 33.0 40.0 32-33 36.364005016636646 40.0 37.0 40.0 33.0 40.0 34-35 36.26404627490882 40.0 37.0 40.0 33.0 40.0 36-37 36.16049134525045 38.5 37.0 40.0 33.0 40.0 38-39 36.07758555257878 37.0 37.0 40.0 33.0 40.0 40-41 35.94413286343178 37.0 37.0 40.0 27.0 40.0 42-43 35.827384133232684 37.0 37.0 40.0 27.0 40.0 44-45 35.6847087201884 37.0 37.0 40.0 27.0 40.0 46-47 35.537093981677124 37.0 37.0 40.0 27.0 40.0 48-49 35.39189563426452 37.0 37.0 40.0 27.0 40.0 50-51 34.258110750639005 37.0 35.0 38.5 24.5 38.5 52-53 34.682639396034276 37.0 35.0 38.5 27.0 40.0 54-55 35.38763698054066 37.0 37.0 40.0 27.0 40.0 56-57 35.428783581002634 37.0 37.0 40.0 27.0 40.0 58-59 35.23788958168369 37.0 37.0 40.0 27.0 40.0 60-61 35.01626483656699 37.0 33.0 40.0 27.0 40.0 62-63 34.766402791510586 37.0 33.0 40.0 27.0 40.0 64-65 34.55396602513344 37.0 33.0 40.0 27.0 40.0 66-67 34.26483361813251 37.0 33.0 40.0 27.0 40.0 68-69 34.042535381083866 37.0 33.0 37.0 27.0 40.0 70-71 33.74453039419215 37.0 33.0 37.0 22.0 40.0 72-73 33.50422070329328 37.0 33.0 37.0 22.0 40.0 74-75 33.26164603838532 37.0 33.0 37.0 22.0 40.0 76-77 32.995854300091494 37.0 33.0 37.0 22.0 37.0 78-79 32.71645143473141 37.0 33.0 37.0 22.0 37.0 80-81 32.414039197666355 37.0 33.0 37.0 22.0 37.0 82-83 32.14131458240166 37.0 33.0 37.0 22.0 37.0 84-85 31.83903850532742 35.0 33.0 37.0 22.0 37.0 86-87 31.668456576501917 33.0 33.0 37.0 15.0 37.0 88-89 31.495720960740794 33.0 33.0 37.0 15.0 37.0 90-91 31.308032490697016 33.0 33.0 37.0 15.0 37.0 92-93 31.11615947283775 33.0 33.0 37.0 15.0 37.0 94-95 30.934104974993744 33.0 30.0 37.0 15.0 37.0 96-97 30.76852057939025 33.0 27.0 37.0 15.0 37.0 98-99 30.5984090564087 33.0 27.0 37.0 15.0 37.0 100-101 29.165291946508354 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.02556414694655018 2306 2 0.09128948915788015 2306 3 0.12875055126526647 2306 4 0.2382981287611159 2306 5 0.23152357013719183 2306 6 0.2103338793939713 2306 7 0.16518297352524058 2306 8 0.18869849495774815 2306 9 0.0999959189713806 2306 10-11 0.06479028535716225 2306 12-13 0.1385563952345663 2306 14-15 0.1447385320322283 2306 16-17 0.1316138980145567 2306 18-19 0.1352447983131171 2306 20-21 -0.027482123580895745 2306 22-23 -0.1579900942412209 2306 24-25 -0.16726342381736004 2306 26-27 -0.19639409326547508 2306 28-29 -0.23859910094927983 2306 30-31 -0.12541865939987673 2306 32-33 -0.2893392700553079 2306 34-35 -0.36697651321198776 2306 36-37 -0.278555508930765 2306 38-39 -0.24776572718076295 2306 40-41 -0.3825679573509788 2306 42-43 -0.34998947776679046 2306 44-45 -0.2138860053331797 2306 46-47 -0.21029720383953077 2306 48-49 -0.32375307578535484 2306 50-51 -0.38510319562085016 2306 52-53 -0.3614267484628684 2306 54-55 -0.20061331096480473 2306 56-57 -0.2801197393413801 2306 58-59 -0.301099259990373 2306 60-61 -0.37054187864105614 2306 62-63 -0.3630354201080124 2306 64-65 -0.25975204974124466 2306 66-67 -0.23904715717200276 2306 68-69 -0.4047859211875533 2306 70-71 -0.4107819083843083 2306 72-73 -0.3735878463139173 2306 74-75 -0.2533293431511652 2306 76-77 -0.44677167466356593 2306 78-79 -0.2870529585079282 2306 80-81 -0.3306094518805409 2306 82-83 -0.22670961044472904 2306 84-85 -0.08279783442204192 2306 86-87 -0.12356985898944473 2306 88-89 -0.21386371744444332 2306 90-91 -0.30962574494165906 2306 92-93 -0.44727377948385083 2306 94-95 -0.29791129302321195 2306 96-97 -0.02369780390293741 2306 98-99 -0.11203383523386279 2306 100-101 -0.15087369979969978 2307 1 0.148323750340964 2307 2 0.2113132704205043 2307 3 0.18285306330069417 2307 4 0.21571821066969932 2307 5 0.194422148774116 2307 6 0.18584280748565618 2307 7 0.21802105927316262 2307 8 0.16568747170053655 2307 9 0.21929774910980626 2307 10-11 0.12833293985686112 2307 12-13 0.09996473723814603 2307 14-15 0.25740282196824893 2307 16-17 0.2295737894637 2307 18-19 0.24855093112950755 2307 20-21 0.23833147402590527 2307 22-23 0.13195052936098506 2307 24-25 0.16160889913330578 2307 26-27 0.11829941943216227 2307 28-29 -0.11335593265427946 2307 30-31 -0.18167048678196096 2307 32-33 -0.07925135335113964 2307 34-35 -0.15568836846448875 2307 36-37 -0.07487632151306656 2307 38-39 -0.03432652256909563 2307 40-41 -0.08119376602207495 2307 42-43 -0.16685889225787065 2307 44-45 -0.06479814375575899 2307 46-47 0.003826689002430328 2307 48-49 -0.14827645615786622 2307 50-51 -0.2700622448169483 2307 52-53 -0.17920959231948785 2307 54-55 -0.10920268713231707 2307 56-57 -0.2936414945213741 2307 58-59 -0.11992141759566977 2307 60-61 -0.03380045591027425 2307 62-63 0.019847723038019183 2307 64-65 -0.051587847380012875 2307 66-67 0.01881632526175281 2307 68-69 -0.11677982793680997 2307 70-71 -0.14831555140438013 2307 72-73 -0.2245048693855054 2307 74-75 -0.1660873485675438 2307 76-77 -0.19363415993111488 2307 78-79 -0.12454933863410389 2307 80-81 -0.13584823794757028 2307 82-83 -0.06986960154070943 2307 84-85 -0.1023973490274912 2307 86-87 0.005573841104268951 2307 88-89 -0.14907010433237033 2307 90-91 -0.19072103676803565 2307 92-93 -0.1202833722367096 2307 94-95 0.09275588722940853 2307 96-97 0.05291882302362083 2307 98-99 -0.15135016580179084 2307 100-101 -0.24573818916895718 2201 1 0.20976974281260397 2201 2 0.1489968158945416 2201 3 0.17347651633895467 2201 4 0.2796906150031262 2201 5 0.18804933845235894 2201 6 0.24326642076866278 2201 7 0.2674671855006494 2201 8 0.25972832099769505 2201 9 0.30351541183133435 2201 10-11 0.33194336415793657 2201 12-13 0.2881422286210622 2201 14-15 0.42054495656727653 2201 16-17 0.3615010472656337 2201 18-19 0.42877762105920425 2201 20-21 0.40462342762836556 2201 22-23 0.4292454485403283 2201 24-25 0.4660545762296451 2201 26-27 0.35498136967105864 2201 28-29 0.32225367599841803 2201 30-31 0.24132948633573648 2201 32-33 0.23668086915301956 2201 34-35 0.21886123823432513 2201 36-37 0.23019990948327518 2201 38-39 0.2122540158244064 2201 40-41 0.3212278704282312 2201 42-43 0.2304508449709104 2201 44-45 0.41868189482362084 2201 46-47 0.3566903426220094 2201 48-49 0.23600074102775892 2201 50-51 0.16812065580968039 2201 52-53 0.2161568823026414 2201 54-55 0.31217162794268205 2201 56-57 0.21275229710931143 2201 58-59 0.3561433674409855 2201 60-61 0.31947548042980856 2201 62-63 0.35919023438267317 2201 64-65 0.4358621826822713 2201 66-67 0.3765216126171751 2201 68-69 0.33795634193835156 2201 70-71 0.3507779904831594 2201 72-73 0.266993766501578 2201 74-75 0.31768839533459925 2201 76-77 0.20528438472661747 2201 78-79 0.34712661554009827 2201 80-81 0.3169392928605319 2201 82-83 0.39216544117920193 2201 84-85 0.3406859329999854 2201 86-87 0.28530307731250204 2201 88-89 0.39872177681900567 2201 90-91 0.24027537214980654 2201 92-93 0.2733794856667231 2201 94-95 0.1769052951303145 2201 96-97 0.10530561083111678 2201 98-99 0.05053638170415553 2201 100-101 0.14274340006028652 1101 1 0.2179216677034006 1101 2 0.09137611212412367 1101 3 0.14760645592673782 1101 4 0.20135172148985703 1101 5 0.20437976269636948 1101 6 0.2848665370766241 1101 7 0.3017903083217348 1101 8 0.3366292143405545 1101 9 0.3785066380878561 1101 10-11 0.33033692002955206 1101 12-13 0.38222958948498587 1101 14-15 0.5091597656929281 1101 16-17 0.4444569259120925 1101 18-19 0.5397124760883614 1101 20-21 0.4775807541659418 1101 22-23 0.543237022171553 1101 24-25 0.48123687644599755 1101 26-27 0.37401845653796784 1101 28-29 0.30982916147392103 1101 30-31 0.41260463748544396 1101 32-33 0.3364315697118556 1101 34-35 0.4293981317511637 1101 36-37 0.35743956763797513 1101 38-39 0.35466155486830786 1101 40-41 0.4118698462812205 1101 42-43 0.3103271843446649 1101 44-45 0.46520317256667454 1101 46-47 0.478757990716538 1101 48-49 0.42525643561925364 1101 50-51 0.10995422221574103 1101 52-53 0.3326903092225777 1101 54-55 0.5197203840373845 1101 56-57 0.5025254191260089 1101 58-59 0.5721417896567615 1101 60-61 0.5893610731982264 1101 62-63 0.537613618002915 1101 64-65 0.47609609753555304 1101 66-67 0.569162810303169 1101 68-69 0.44265442089072593 1101 70-71 0.5875364899912938 1101 72-73 0.20895001635634003 1101 74-75 0.2892410887397787 1101 76-77 0.3609472179575661 1101 78-79 0.3575854540215886 1101 80-81 0.4698157996653194 1101 82-83 0.42810180070426895 1101 84-85 0.5446345048609693 1101 86-87 0.4031708282490136 1101 88-89 0.4121044020064737 1101 90-91 0.19053107687058812 1101 92-93 0.327982617520739 1101 94-95 0.2962823682809912 1101 96-97 0.30145678258020325 1101 98-99 0.3083647952862094 1101 100-101 0.34445119656664147 2304 1 0.09675799077975711 2304 2 0.04622582043257495 2304 3 0.09702750371332414 2304 4 0.12787355154965496 2304 5 0.15294975926875054 2304 6 0.13613870450094367 2304 7 0.23745994534296955 2304 8 0.2122384603465619 2304 9 0.1602353644660255 2304 10-11 0.17199144608490258 2304 12-13 0.18595465450613347 2304 14-15 0.2664424944017938 2304 16-17 0.2564811098164981 2304 18-19 0.36720494278667815 2304 20-21 0.23983675634346469 2304 22-23 0.17021342292848374 2304 24-25 0.21191139173188134 2304 26-27 0.07773764594364252 2304 28-29 0.08272969503780558 2304 30-31 0.19899094384260962 2304 32-33 0.12137990779885399 2304 34-35 0.26741670449649746 2304 36-37 0.23883355579845045 2304 38-39 0.21645563914396604 2304 40-41 0.19198997522681083 2304 42-43 0.25136119384640665 2304 44-45 0.07352879079796537 2304 46-47 0.11028525437796333 2304 48-49 0.17710760359386768 2304 50-51 0.22405545230323298 2304 52-53 0.22667143399582557 2304 54-55 0.31944852672095436 2304 56-57 0.42508985566899327 2304 58-59 0.3832614832482548 2304 60-61 0.37940379547506353 2304 62-63 0.2225651033574323 2304 64-65 0.35792447306321407 2304 66-67 0.4695510413272146 2304 68-69 0.29825735732354985 2304 70-71 0.14112736648166901 2304 72-73 0.24513736277264542 2304 74-75 0.43153313518656944 2304 76-77 0.21076551965119705 2304 78-79 0.3592920141838576 2304 80-81 0.27281516709677334 2304 82-83 0.21940310774405702 2304 84-85 0.40811409590523695 2304 86-87 0.44079351126127975 2304 88-89 0.1646254116284105 2304 90-91 0.13085088221692232 2304 92-93 0.21315155435551247 2304 94-95 0.289157996539501 2304 96-97 0.23739941551976074 2304 98-99 0.15386736610966167 2304 100-101 0.3373076511987776 2202 1 0.08188147952129299 2202 2 0.05076697920137008 2202 3 0.04122956001964795 2202 4 0.05680437795816573 2202 5 0.01712759036220035 2202 6 0.07385476510982869 2202 7 0.07784264463945334 2202 8 0.15931488051760567 2202 9 0.13798617352751563 2202 10-11 0.0915828000854475 2202 12-13 0.06724047354682483 2202 14-15 0.20411032025694453 2202 16-17 0.14708031660981646 2202 18-19 0.15594721356813324 2202 20-21 0.1211329703864763 2202 22-23 0.17578757290874591 2202 24-25 0.18737214292332993 2202 26-27 0.1030577828701098 2202 28-29 -0.00881671019552499 2202 30-31 0.05278543056654428 2202 32-33 0.04973857190699249 2202 34-35 0.08282584790167391 2202 36-37 0.1582607270521308 2202 38-39 0.026096673483614552 2202 40-41 0.010717310235783373 2202 42-43 0.11737670591004701 2202 44-45 0.12478795588693004 2202 46-47 0.12880283378412116 2202 48-49 0.19903435516147283 2202 50-51 0.14766855498020703 2202 52-53 0.06924777022965856 2202 54-55 0.12402748638135819 2202 56-57 0.35831551815122253 2202 58-59 0.23773718264732224 2202 60-61 0.16009358256987838 2202 62-63 0.27792859424403105 2202 64-65 0.290070901146116 2202 66-67 0.22763577866590623 2202 68-69 0.21550779566234013 2202 70-71 0.2190269795155615 2202 72-73 0.16451527920226283 2202 74-75 0.23929383990166997 2202 76-77 0.12524005519860282 2202 78-79 0.19245212986177762 2202 80-81 0.24222690128626567 2202 82-83 0.3497614399893223 2202 84-85 0.26500347598227236 2202 86-87 0.2503044137728665 2202 88-89 0.3064893598951066 2202 90-91 0.344974377564629 2202 92-93 0.2485927804295187 2202 94-95 0.21668083445673858 2202 96-97 0.3433543140334514 2202 98-99 0.155590896353484 2202 100-101 0.30319883126275826 1102 1 0.13905498293265595 1102 2 0.12735743297629654 1102 3 0.08070869604004471 1102 4 0.14717899794443667 1102 5 0.1726294711674825 1102 6 0.11735057620273182 1102 7 0.19285914108798607 1102 8 0.19356760190624556 1102 9 0.12762353185419784 1102 10-11 0.15305860179182673 1102 12-13 0.1209755094117142 1102 14-15 0.1669165640401289 1102 16-17 0.17816937991975124 1102 18-19 0.21754989082614173 1102 20-21 0.1486136982616202 1102 22-23 0.25571978336279955 1102 24-25 0.22817489128068758 1102 26-27 0.25729401656629847 1102 28-29 0.19501683153243476 1102 30-31 0.2536998276581244 1102 32-33 0.20242252832274232 1102 34-35 0.19553818276207124 1102 36-37 0.2099855866046596 1102 38-39 0.15121326064511464 1102 40-41 0.06924752395409683 1102 42-43 0.14564823885391576 1102 44-45 0.2552193064942472 1102 46-47 0.3563989229700155 1102 48-49 0.205802539781601 1102 50-51 0.25970049889928504 1102 52-53 0.3234918406145084 1102 54-55 0.4310503414927993 1102 56-57 0.5536151248282621 1102 58-59 0.5293788944953448 1102 60-61 0.42485251902177623 1102 62-63 0.5318011442937589 1102 64-65 0.43626073814299815 1102 66-67 0.4668816965829379 1102 68-69 0.345994090651061 1102 70-71 0.45977867994566424 1102 72-73 0.38051543330920623 1102 74-75 0.40153908184957743 1102 76-77 0.47182776832151063 1102 78-79 0.39854539608853656 1102 80-81 0.4981092774052982 1102 82-83 0.37361172333596926 1102 84-85 0.48293101769622027 1102 86-87 0.3443469542959896 1102 88-89 0.3360065760931512 1102 90-91 0.2592168806117421 1102 92-93 0.12470471461184474 1102 94-95 0.3381198256166016 1102 96-97 0.3609190914890057 1102 98-99 0.41934012581873503 1102 100-101 0.384524571326363 2305 1 0.09695312660760891 2305 2 0.060791729047352305 2305 3 0.10969329034192654 2305 4 0.1055631678712814 2305 5 0.11182164296359076 2305 6 0.1637575183974178 2305 7 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94-95 0.3115200253373054 1213 96-97 0.3367320878033446 1213 98-99 0.19755842597078654 1213 100-101 0.4428694990535611 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1862.0 3 353.0 4 349.0 5 415.0 6 1248.0 7 4060.0 8 11937.0 9 15768.0 10 16161.0 11 14674.0 12 11942.0 13 10665.0 14 10371.0 15 10332.0 16 11079.0 17 11917.0 18 13615.0 19 15014.0 20 16935.0 21 19279.0 22 22130.0 23 24884.0 24 28188.0 25 33046.0 26 38724.0 27 45848.0 28 54851.0 29 66082.0 30 79915.0 31 99777.0 32 129670.0 33 176904.0 34 256591.0 35 398130.0 36 662188.0 37 1063088.0 38 519106.0 39 2726.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16241338690014 24.493953158195257 16.098308118868925 16.24532533603568 2 37.6248169146205 21.45050934764005 28.37087955039168 12.553794187347773 3 43.108642730630905 19.30798516229702 22.09172538424561 15.491646722826463 4 36.06793889303711 19.713048652441618 28.619284395156992 15.599728059364281 5 37.15880733841191 17.458828621986243 28.950390764368212 16.43197327523363 6 33.65828042152687 17.918822832414346 31.67316920525157 16.749727540807218 7 40.35407944122296 18.06045028233178 22.229884278129035 19.35558599831623 8 34.51999447316015 26.243834311916142 20.48657250150627 18.74959871341743 9 33.75410764639757 22.712055720714453 23.161682200275973 20.372154432612003 10-11 29.107988874346557 28.342021933988544 27.83174528682138 14.71824390484352 12-13 29.46686605099245 27.36018328224827 26.801947399566266 16.371003267193014 14-15 27.913169977607495 27.87562657928796 27.369586765823694 16.84161667728085 16-17 27.324222862454484 27.894025297733684 27.658842651794107 17.12290918801773 18-19 26.600880816131717 28.25150924520602 27.703191125957837 17.444418812704427 20-21 25.801676735019175 28.619446059169455 27.800476646283474 17.778400559527892 22-23 26.06327991988882 28.618390384809487 27.301713149487526 18.016616545814156 24-25 24.892416352198662 29.21492435230595 27.654400523230745 18.238258772264647 26-27 24.64943260192317 29.104235103190447 27.686353470723567 18.559978824162812 28-29 24.489588020831796 29.261192220847544 27.486384068129695 18.762835690190965 30-31 24.534316716454338 28.584316902908963 27.980049612360837 18.901316768275862 32-33 24.052950689018058 28.61395540046811 28.27704838999302 19.05604552052082 34-35 23.366960581880342 29.342770626111058 28.23551689400361 19.054751898004994 36-37 23.596655331860784 29.088517990852917 27.905142132703155 19.409684544583143 38-39 23.146178421173484 29.009137879911524 28.41433631540254 19.43034738351245 40-41 23.122761915396477 28.914059094953192 28.342763798745153 19.620415190905174 42-43 23.436072700998437 28.472271898481683 28.25184225301664 19.83981314750324 44-45 22.91854953919629 28.757994892221866 28.414556370148492 19.908899198433346 46-47 23.227622525804353 28.652152466263864 27.950527280320454 20.169697727611332 48-49 22.723381557638362 28.835828944978182 28.07699472676925 20.363794770614206 50-51 22.451556491522773 28.384689730984906 28.599553905947644 20.564199871544673 52-53 23.24771822397746 28.358446269722325 27.942947559351904 20.450887946948306 54-55 22.946004445229377 28.469212882598377 28.334973592703122 20.24980907946912 56-57 23.157307452637905 28.255065018197683 28.19453899625343 20.393088532910976 58-59 22.881599244257064 28.654198211696517 27.969820706574367 20.494381837472048 60-61 22.34537430158689 28.38391006401622 28.144347583194207 21.126368051202686 62-63 22.67392803219189 28.484212020626305 27.951413847171857 20.890446100009953 64-65 22.21489057658199 28.72683651507607 28.30804965823186 20.750223250110082 66-67 22.17316648724152 28.318162583619195 28.144435312411975 21.364235616727314 68-69 21.940882038128947 28.447877969263146 28.593237778838322 21.018002213769584 70-71 21.80460689086941 28.67033906085218 28.489132355085424 21.03592169319299 72-73 21.815334491355337 29.17737151291589 27.88620698078978 21.121087014938997 74-75 21.458225447387054 29.44570236386572 28.16439015535062 20.931682033396605 76-77 21.638546180688852 29.213862344913487 28.014632920883543 21.13295855351412 78-79 21.47593293554039 29.561565008827483 27.922742433763 21.039759621869123 80-81 21.367711064113177 29.43365059296995 28.080428963386915 21.118209379529958 82-83 21.58098007424345 29.50221493043953 27.74583335584148 21.170971639475535 84-85 21.373349359497855 29.483212781286323 27.942743586426182 21.200694272789637 86-87 21.623967535773808 29.078660517569975 27.918933576971934 21.37843836968428 88-89 21.560274164823266 29.415072428650124 27.706886313379332 21.317767093147282 90-91 21.503696835087332 29.11828972023916 27.936035557521826 21.44197788715168 92-93 21.59496945080368 29.104313714194895 27.70482157304573 21.595895261955693 94-95 21.330838382638312 29.17032474475744 27.982227269384214 21.516609603220036 96-97 21.475186068158674 28.865622742226833 27.928395298610553 21.730795891003936 98-99 21.382048636592447 29.159113901486478 27.77004817050099 21.688789291420086 100-101 21.447879859338936 28.93278780999181 27.920902202182656 21.698430128486592 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1077.0 1 819.5 2 420.0 3 311.5 4 316.5 5 321.5 6 376.5 7 521.5 8 783.5 9 1002.5 10 1206.0 11 1393.0 12 1430.5 13 1326.0 14 1160.5 15 1010.5 16 898.0 17 835.5 18 864.5 19 1014.5 20 1300.0 21 1736.0 22 2508.5 23 3688.5 24 5267.0 25 7567.0 26 10562.0 27 14505.5 28 19328.5 29 25310.0 30 32564.5 31 41149.0 32 51461.5 33 63396.0 34 76567.5 35 91248.0 36 107973.0 37 125401.0 38 141303.0 39 155088.5 40 167372.5 41 178364.0 42 186557.0 43 191470.5 44 193843.5 45 194098.0 46 193420.0 47 191106.0 48 185921.0 49 176969.0 50 165316.0 51 151378.5 52 133808.0 53 114302.5 54 95064.0 55 77438.0 56 63064.5 57 51160.5 58 41086.0 59 33089.0 60 26548.0 61 20987.5 62 16227.5 63 12506.0 64 9694.0 65 7519.5 66 5729.0 67 4335.5 68 3356.0 69 2601.0 70 2050.0 71 1646.5 72 1337.5 73 1076.5 74 859.0 75 691.0 76 567.0 77 498.0 78 410.0 79 309.5 80 261.5 81 235.0 82 190.0 83 159.0 84 136.5 85 116.0 86 96.0 87 77.5 88 60.0 89 49.0 90 46.5 91 40.5 92 32.0 93 19.5 94 14.5 95 14.5 96 11.0 97 6.5 98 2.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30370601339957903 2 0.28021777393031067 3 0.24960100763521637 4 0.23767739262079518 5 0.2436007368537657 6 0.19226508683468793 7 0.15841740550342784 8 0.15636603087729087 9 0.13482659730285265 10-11 0.12005669999466642 12-13 0.14109611100398378 14-15 0.14523732353049779 16-17 0.13857035599555262 18-19 0.1259928653190503 20-21 0.13108283861015266 22-23 0.15987900992455045 24-25 0.5737694829305118 26-27 0.22043302466983125 28-29 0.19489341057442594 30-31 0.29433379557641576 32-33 0.32560443753359125 34-35 0.3345150960658737 36-37 0.32519416260836387 38-39 0.3328739963649642 40-41 0.3366818605147309 42-43 0.34745157730195003 44-45 0.32850200419300973 46-47 0.3477336413130439 48-49 0.3436565342435966 50-51 0.3499645112189678 52-53 0.33460484370576726 54-55 0.32656601938959295 56-57 0.33504076081382134 58-59 0.32791223398799535 60-61 0.30521890218635506 62-63 0.29209010457907847 64-65 0.3027828948178174 66-67 0.31819384669667145 68-69 0.3047701639868876 70-71 0.28644882435720176 72-73 0.2822947907392744 74-75 0.2740123656862463 76-77 0.27655094178609085 78-79 0.2850769675759727 80-81 0.2942055846622822 82-83 0.3165014626301084 84-85 0.30809082666294685 86-87 0.3102319489289773 88-89 0.31561680732258685 90-91 0.3087062390507879 92-93 0.2908336376205696 94-95 0.286423182174375 96-97 0.3042957836045935 98-99 0.3121935759152208 100-101 0.31234742901218104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3899824.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.54247247607661 #Duplication Level Percentage of deduplicated Percentage of total 1 97.23764545092102 91.93087418682197 2 2.0141108120563724 3.8083803202521573 3 0.365777319119222 1.0374447637560642 4 0.09925844400853212 0.3753655484277935 5 0.08478758879073053 0.40080141397802715 6 0.046415850594090036 0.2632961566347265 7 0.034136464500937096 0.22591420288572886 8 0.02325561210818741 0.17589144541220977 9 0.010346435525372916 0.08803598362947632 >10 0.0808437184737081 1.2335910116795084 >50 0.002570078008201446 0.17476979672905285 >100 7.553234206211355E-4 0.1009652369656579 >500 6.977924004215583E-5 0.04832623423696794 >1k 0.0 0.0 >5k 2.7123233003533366E-5 0.13634369859068338 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT 5317 0.13633948608962865 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.718342161082142E-4 0.0 0.0 2 0.0011026138615486238 0.0 0.0 3 0.0015385309696027308 0.0 0.0 4 0.0022052277230972476 0.0 0.0 5 0.0027437135624582033 0.0 0.0 6 0.004846372554248602 0.0 0.0 7 0.00741059083691982 0.0 0.0 8 0.009897882571110902 0.0 0.0 9 0.011744119734634179 0.0 2.564218282671218E-5 10-11 0.016346891552029015 0.0 3.846327424006827E-5 12-13 0.021398401568891313 0.0 5.128436565342436E-5 14-15 0.02518062353583136 0.0 8.974763989349262E-5 16-17 0.029398762610825514 0.0 1.0256873130684872E-4 18-19 0.03409128206811384 0.0 1.153898227202048E-4 20-21 0.0398864153869508 0.0 1.282109141335609E-4 22-23 0.04588668616840145 0.0 1.4103200554691698E-4 24-25 0.05215619986953257 0.0 1.5385309696027308E-4 26-27 0.05760516372020891 0.0 1.5385309696027308E-4 28-29 0.06319515957643217 0.0 1.5385309696027308E-4 30-31 0.0913887395944022 0.0 1.5385309696027308E-4 32-33 0.10053017777212511 0.0 1.5385309696027308E-4 34-35 0.10638941654802883 0.0 1.5385309696027308E-4 36-37 0.11413335576169592 0.0 1.5385309696027308E-4 38-39 0.12219782226069689 0.0 2.0513746261369744E-4 40-41 0.1320572415575677 0.0 2.1795855402705354E-4 42-43 0.14332698090990773 0.0 2.6924291968047787E-4 44-45 0.15593011376923677 1.282109141335609E-5 3.4616946816061445E-4 46-47 0.17041794706632915 2.564218282671218E-5 3.589905595739705E-4 48-49 0.1874571775546794 2.564218282671218E-5 3.974538338140388E-4 50-51 0.2075478277994084 2.564218282671218E-5 4.23096016640751E-4 52-53 0.22886930281981954 2.564218282671218E-5 4.359171080541071E-4 54-55 0.25211394155223416 2.564218282671218E-5 4.615592908808192E-4 56-57 0.277384312727959 2.564218282671218E-5 4.615592908808192E-4 58-59 0.3050522279979815 2.564218282671218E-5 4.872014737075314E-4 60-61 0.33579720520720935 2.564218282671218E-5 5.128436565342436E-4 62-63 0.36919614833900194 2.564218282671218E-5 5.641280221876679E-4 64-65 0.40608242833522745 2.564218282671218E-5 5.76949113601024E-4 66-67 0.44589191717369814 2.564218282671218E-5 6.025912964277363E-4 68-69 0.4859193645661958 2.564218282671218E-5 6.154123878410923E-4 70-71 0.5296264651943268 2.564218282671218E-5 6.154123878410923E-4 72-73 0.5783337914736666 2.564218282671218E-5 6.282334792544484E-4 74-75 0.6305925600745059 2.564218282671218E-5 6.795178449078728E-4 76-77 0.6866335506422854 5.128436565342436E-5 7.05160027734585E-4 78-79 0.7474824504900734 5.128436565342436E-5 7.692654848013654E-4 80-81 0.8117417606538142 5.128436565342436E-5 7.949076676280776E-4 82-83 0.8803602418980959 5.128436565342436E-5 7.949076676280776E-4 84-85 0.954248191713267 5.128436565342436E-5 8.077287590414336E-4 86-87 1.037021157877894 5.128436565342436E-5 8.205498504547898E-4 88-89 1.1253456566245041 5.128436565342436E-5 8.46192033281502E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGGA 815 0.0 53.016144 2 GATCGGG 1050 0.0 45.228607 1 GAGCGGC 825 0.0 41.949715 9 CGGACGC 65 1.3304071E-4 36.5308 1 TCGGGAG 1080 0.0 36.48243 3 CGATCGC 55 0.0025830325 34.538216 1 AGCGGCG 595 0.0 32.270973 10-11 TTGTGTT 33340 0.0 31.351404 1 GTGTTTG 73925 0.0 31.073294 1 CGCCGGA 200 0.0 30.87131 58-59 GGTTCGG 370 0.0 30.804354 1 TGTTTGA 75110 0.0 30.558584 2 GTTTGAT 17820 0.0 29.915857 3 GGCGCCG 415 0.0 29.188326 56-57 GGATCGG 760 0.0 28.119104 1 TGACGCG 85 6.461866E-4 27.902655 6 GATCCGG 410 0.0 27.79905 1 GTTTGAC 8385 0.0 27.514563 3 GTTTGAG 51005 0.0 27.186182 3 ATCCGGA 390 0.0 26.78443 2 >>END_MODULE