FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_34211-1.34200000035897.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_34211-1.34200000035897.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3899824
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT53170.13633948608962865Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA8150.053.0161442
GATCGGG10500.045.2286071
GAGCGGC8250.041.9497159
CGGACGC651.3304071E-436.53081
TCGGGAG10800.036.482433
CGATCGC550.002583032534.5382161
AGCGGCG5950.032.27097310-11
TTGTGTT333400.031.3514041
GTGTTTG739250.031.0732941
CGCCGGA2000.030.8713158-59
GGTTCGG3700.030.8043541
TGTTTGA751100.030.5585842
GTTTGAT178200.029.9158573
GGCGCCG4150.029.18832656-57
GGATCGG7600.028.1191041
TGACGCG856.461866E-427.9026556
GATCCGG4100.027.799051
GTTTGAC83850.027.5145633
GTTTGAG510050.027.1861823
ATCCGGA3900.026.784432
Produced by FastQC (version 0.11.2)